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joining gene
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  “joining gene”译为未确定词的双语例句
     Because of variable V (variable gene), D (diversity gene), J (joining gene) region of Ig, we first analyzed the Ig Ca mRNA.
     考虑到Ig V(variable gene),D(diversity gene),J(joining gene)区基因片段众多,重组后的VDJ基因序列变异性较大,因此,我们首先分析了Ig保守的恒定区C区(constant gene)的表达。
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  相似匹配句对
     The gene of PNGase F from F.
     为了获得大量廉价的N-糖酰胺酶F,本论文研究的主要内容为根据目的基因N-糖酰胺酶F的cDNA序列设计引物,以F.
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     As to the RYR gene.
     长白猪群中,未发现有该基因存在。
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     Joining Bach
     走在巴赫的小路上
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     Joining Hollywood
     走进好莱坞
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     The Developing Strategy of Gene Industry in Our Country after Joining WTO
     入世后我国基因产业的发展战略
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  joining gene
The fourth framework of the variable region, a segment specified by the joining gene, is also recognized and cross-reacts antigenically with the homologous region of T cell receptor β chains.
      
The chromosomal t(8;14) was seen, and identical rearrangement of immunoglobulin heavy chain joining gene and c-myc gene were detected by Southern blot analysis in the bone marrow lymphoblasts throughout the clinical course.
      
The cosmid contains the TCRAC gene, six putative joining gene segments (TCRAJ), and surprisingly, a chicken homologue for the human apoptotic suppressor gene, defender against cell death (DAD1).
      
The human T cell antigen receptor is encoded by variable, diversity joining gene segments that rearrange to generate a complete V gene.
      


A synthetic gene for a-hANF was reconstructed as a double joined gene,linked in tandem.The two copies in the gene were seperated by codons specifying a Glu-Lys-Phe-Glu linker with Glu residues flanking the authentic N and C termini of the 28-a.a.hormone.This linker is provided for enzymatic cleavage of expressed product by endoproteinaese Glu-c or Lys-c.The double joined gene fragment was cloned and expressed in) secretive expression vector PIN-*******III-OmpA2 in E.coli.The product was...

A synthetic gene for a-hANF was reconstructed as a double joined gene,linked in tandem.The two copies in the gene were seperated by codons specifying a Glu-Lys-Phe-Glu linker with Glu residues flanking the authentic N and C termini of the 28-a.a.hormone.This linker is provided for enzymatic cleavage of expressed product by endoproteinaese Glu-c or Lys-c.The double joined gene fragment was cloned and expressed in) secretive expression vector PIN-*******III-OmpA2 in E.coli.The product was located in periplasmic space of the bacteria and purified by gel filtration and HPLC.The expressed protein after gently oxidized exhibited Vaso-dilatation activity similar to natural *********a-ANF.

将单拷贝人α心钠素基因3′端用Ban Ⅱ酶解除去包括终止密码在内的36个碱基对,代之以人工合成的含Glu-Lys-Phe-Glu连接片段与另一单拷贝人α心钠素基因的5′端串连成编码60肽的双拷贝心钠素基因,克隆于大肠杆菌分泌型表达载体pIN-Ⅲ-OmpA_2质粒中,表达生成60肽的双拷贝人α型心钠素衍生物,在信号肽的作用下分泌至胞膜间质并自动切割为60肽的外源基因产物。分子量约8K的表达产物用分子筛或超滤膜分离后再经HPLC纯化,表达产物具有明显的心钠素放免活性和舒张血管活性。

Objective: To demonstrate the possibility of high-level expression of bioactive human beta-defensin-2(hBD2) in E.coli,and to purify the recombinant hBD2.Methods: DNA fragment containing mature hBD2 coding region(smhBD2-cDNA) was amplified by PCR,multiple copies of smhBD2-cDNA were linked using Bgl Ⅱ and BamH Ⅰ enzymes,pET32-nsmHBD2-cDNA with 1,2,4,or 8 copies of smhBD2-cDNA was constructed.The soluble and insoluble hBD2 proteins were separated and analyzed by SDS-PAGE analysis.The soluble protein underwent a...

Objective: To demonstrate the possibility of high-level expression of bioactive human beta-defensin-2(hBD2) in E.coli,and to purify the recombinant hBD2.Methods: DNA fragment containing mature hBD2 coding region(smhBD2-cDNA) was amplified by PCR,multiple copies of smhBD2-cDNA were linked using Bgl Ⅱ and BamH Ⅰ enzymes,pET32-nsmHBD2-cDNA with 1,2,4,or 8 copies of smhBD2-cDNA was constructed.The soluble and insoluble hBD2 proteins were separated and analyzed by SDS-PAGE analysis.The soluble protein underwent a separation process containing affinity chromatography,enterokinase digestion and ion exchange chromatography to get the recombinant hBD2 peptide.The bioactivity of recombinant hBD2 was examined by bacteria-inhibition tests in liquid culture.Results: The plasmids pET32-nsmHBD2-cDNA with 1,2,4 copies of smhBD2-cDNA were constructed and the expressed soluble protein accounted for 52%,48%,and 31% respectively.The plasmids with 8 copies expressed mainly insoluble protein with few in soluble form.The growth of E.coli K12D31 was dramatically suppressed with a inhibition rate of 90%,when the final concentration of recombinant hBD2 reached between 0.4 to 0.5 μg/ml.Conclusion: Fusion expression of human β-defensin-2 with multiple joined genes in E.coli could increase the expression of hBD2.

目的:研究具有生物学活性的人防御素2(HBD 2)多肽在大肠杆菌中的原核高效表达的可行性及融合表达蛋白的分离纯化技术。方法:PCR扩增不含前导序列的HBD 2成熟肽的编码框全长的cDNA片段(smHBD 2-cDNA),利用B g lⅡ和B amHⅠ同尾酶构建多拷贝串联的nsmHBD 2-cDNA的克隆载体,利用N colⅠ和H indⅢ限制性内切酶构建融合表达载体pET 32-nsmHBD 2-cDNA,在大肠杆菌中BL 21(DE 3)中诱导表达含HBD 2的融合蛋白,SDS-PAGE分析产量。可溶蛋白经亲和层析、肠激酶酶切、离子交换层析等方法分离、纯化HBD 2多肽。采用液体培养法,测定重组人β防御素2对大肠杆菌的抑菌活性。结果:构建了n为1、2、4或8倍的多拷贝串联nsmHBD 2-cDNA表达载体,1、2和4倍smHBD 2-cDNA的可溶蛋白比例分别为52%、48%和31%;而8倍HBD 2-cDNA几乎不含可溶蛋白,目标蛋白以包含体形式表达,集中在不可溶部分。重组HBD 2对E.coli K 12 D 31有较强的抑制作用,在终浓度约为0.4至0.5μg/m l时,90%细胞的生长被抑制。结论:...

目的:研究具有生物学活性的人防御素2(HBD 2)多肽在大肠杆菌中的原核高效表达的可行性及融合表达蛋白的分离纯化技术。方法:PCR扩增不含前导序列的HBD 2成熟肽的编码框全长的cDNA片段(smHBD 2-cDNA),利用B g lⅡ和B amHⅠ同尾酶构建多拷贝串联的nsmHBD 2-cDNA的克隆载体,利用N colⅠ和H indⅢ限制性内切酶构建融合表达载体pET 32-nsmHBD 2-cDNA,在大肠杆菌中BL 21(DE 3)中诱导表达含HBD 2的融合蛋白,SDS-PAGE分析产量。可溶蛋白经亲和层析、肠激酶酶切、离子交换层析等方法分离、纯化HBD 2多肽。采用液体培养法,测定重组人β防御素2对大肠杆菌的抑菌活性。结果:构建了n为1、2、4或8倍的多拷贝串联nsmHBD 2-cDNA表达载体,1、2和4倍smHBD 2-cDNA的可溶蛋白比例分别为52%、48%和31%;而8倍HBD 2-cDNA几乎不含可溶蛋白,目标蛋白以包含体形式表达,集中在不可溶部分。重组HBD 2对E.coli K 12 D 31有较强的抑制作用,在终浓度约为0.4至0.5μg/m l时,90%细胞的生长被抑制。结论:采用融合表达、多拷贝串联表达方式,可增加产物长度以弱化蛋白酶的降解作用,也减弱了目标蛋白产物对宿主的毒性;适当的多拷贝串联基因可以提高HBD 2的产量,但拷贝数过高会影响重组蛋白表达产量,且易形成不溶蛋白;在大肠杆菌中可达到具有生物学活性的HBD 2多肽的原核高效表达。

 
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