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major trna
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     These results confirm that these eluted proteins contain two major tRNA specific binding proteins.
     凝胶阻滞电泳实验鉴定出两种主要的与tRNA分子特异性结合的蛋白质
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     the major patterns;
     校本管理的主要模式;
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     var. major N.
     var. major N.
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     The discriminator base G73 was one of the major identity elements of tRNA Trp .
     识别位碱基G73 , 为tRNATrp的主要个性元件之一。
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  major trna
The results show that deletion of the major tRNA3bValloci resulted in a reduction of approximately 50% in the level of tRNA3bValbut did not produce the Minute phenotype; furthermore the effects of deficiencies at two loci were approximately additive.
      
The level of tRNA3bValin the tRNA isolated from flies deficient in the major tRNA3bValloci has been examined.
      
chloramphenicol inhibition, Su-7 and Su+7 tRNA can be elevated similarly in the plasmid-containing cell; Su+7 reaches levels of molecules/cell which ordinarily characterize a major tRNA.
      
Chromosomal locations of major tRNA gene clusters of Xenopus laevis
      
Chromosomal location of a major tRNA gene cluster of Xenopus laevis
      
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Crude extract of total membrane proteins of yeast was obtained by a method of phase separation in Triton X 114. The membrane extract was applied to the affinity column on which yeast total tRNAs were covalently coupled to hydrazinyl Sepharose 4B. By eluting with increasing concentration gradient of (NH 4) 2SO 4, eluted proteins were found between concentrations of (NH 4) 2SO 4 0.1 mol/L to 0.3 mol/L. By gel mobility shift assays, it was also observed that there were more than two mobility shift...

Crude extract of total membrane proteins of yeast was obtained by a method of phase separation in Triton X 114. The membrane extract was applied to the affinity column on which yeast total tRNAs were covalently coupled to hydrazinyl Sepharose 4B. By eluting with increasing concentration gradient of (NH 4) 2SO 4, eluted proteins were found between concentrations of (NH 4) 2SO 4 0.1 mol/L to 0.3 mol/L. By gel mobility shift assays, it was also observed that there were more than two mobility shift bands on the gel electropherogram after incubation of sample of 32 P labeled tRNA with the eluent proteins and the reaction mixtures were analyzed by non denaturing polyacrylamide gel electrophoresis. These results confirm that these eluted proteins contain two major tRNA specific binding proteins.

用Triton X114 分相法分离啤酒酵母的膜总蛋白。经过酵母tRNA 分子交联的Sepharose4B亲和柱层析,用0~0 .8 mol/L(NH4)2SO4 梯度缓冲液洗脱tRNA 结合的蛋白质。凝胶阻滞电泳实验鉴定出两种主要的与tRNA分子特异性结合的蛋白质

 
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