助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   mismatch repair genes 在 生物学 分类中 的翻译结果: 查询用时:0.089秒
图标索引 在分类学科中查询
所有学科
生物学
肿瘤学
妇产科学
皮肤病与性病
基础医学
内分泌腺及全身性疾病
神经病学
感染性疾病及传染病
心血管系统疾病
更多类别查询

图标索引 历史查询
 

mismatch repair genes
相关语句
  错配修复基因
    Identification and Analysis of Mismatch Repair Genes in the Extremely Radioresistant Bacterium Deinococcus Radiodurans
    极端耐辐射异常球菌Deinococcus radiodurans错配修复基因的功能鉴定和分析
短句来源
    The microsatellite instability (MI) is highly polymorphic, which is associated with the defects in DNA mismatch repair genes.
    微卫星的高度多态性是微卫星不稳定性的表现,它与错配修复基因的缺陷有关。
短句来源
  错配修复基因
    Identification and Analysis of Mismatch Repair Genes in the Extremely Radioresistant Bacterium Deinococcus Radiodurans
    极端耐辐射异常球菌Deinococcus radiodurans错配修复基因的功能鉴定和分析
短句来源
    The microsatellite instability (MI) is highly polymorphic, which is associated with the defects in DNA mismatch repair genes.
    微卫星的高度多态性是微卫星不稳定性的表现,它与错配修复基因的缺陷有关。
短句来源
  “mismatch repair genes”译为未确定词的双语例句
    Defects in human mismatch repair genes cause genome instability and hereditary nonpolyposis colon cancer (HNPCC) and others tumors.
    人体内MMR基因缺陷会造成基因组的不稳定并诱发遗传性非息肉型直肠癌以及其他自发性肿瘤 .
短句来源
查询“mismatch repair genes”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  mismatch repair genes
Sequence divergence of 15% decreased spontaneous recombination by six-fold in mismatch repair proficient cells but only three- and two- fold in human cells with defects in mismatch repair genes MLH1 and MSH2, respectively.
      
The mismatch repair genes, hMLH1 (3p22) and hMSH2 (2p21), are commonly associated with accumulation of mutations and microsatellite instability.
      
Most patients with HNPCC have a mutation in one of two DNA mismatch repair genes, hMSH2 or hMLH1.
      
The cellular DNA mismatch repair (MMR) pathway, involving the DNA mismatch repair genes MLH1 and MSH2, detects and repairs DNA replication errors.
      
Germline mutations in mismatch repair genes, predominantly MSH2 and MLH1, have been found to underlie the Lynch syndrome (also called hereditary non-polyposis colorectal cancer, HNPCC), a hereditary predisposition for cancer.
      
更多          


Microsatellites are simply repeated nucleotide sequences scattered throughout the human genome. They are highly polymorphic among human population and inherited in a stable manner. The microsatellite instability (MI) is highly polymorphic, which is associated with the defects in DNA mismatch repair genes. MI has been widely used by scientists to study the tumorigenesis. On the basis of their findings, a “mutator that mutates the other mutator” model for tumorigenesis has been proposed. MI is also a potential...

Microsatellites are simply repeated nucleotide sequences scattered throughout the human genome. They are highly polymorphic among human population and inherited in a stable manner. The microsatellite instability (MI) is highly polymorphic, which is associated with the defects in DNA mismatch repair genes. MI has been widely used by scientists to study the tumorigenesis. On the basis of their findings, a “mutator that mutates the other mutator” model for tumorigenesis has been proposed. MI is also a potential tool for the study of genetics, aging and other life sciences.

微卫星为遍布于人类基因组中的简单重复序列。在人群中,它们呈现高度多态性,并且稳定遗传。微卫星的高度多态性是微卫星不稳定性的表现,它与错配修复基因的缺陷有关。微卫星不稳定性已广泛应用于肿瘤学的研究,并依此提出了肿瘤发生的“增变基因”途径。在遗传学、老年病学及其它一些生命科学,微卫星不稳定性同样具有广泛的应用前景。

DNA mismatch repair gene mutS (2.56kb) was PCR modified and cloned into a secretive prokaryotic expression vector pET32a (+) which carries a N-terminal His .tag +and thioredoxin sequence. MutS protein was expressed with high level after IPTG induction using the strain E.coli AD494(DE3) . SDS-PAGE revealed that the expected protein with a molecular weight of 108kD which is about 35% of the total bacterial proteins is almost soluble. The expected protein was purified directly by immobilized metal (Ni...

DNA mismatch repair gene mutS (2.56kb) was PCR modified and cloned into a secretive prokaryotic expression vector pET32a (+) which carries a N-terminal His .tag +and thioredoxin sequence. MutS protein was expressed with high level after IPTG induction using the strain E.coli AD494(DE3) . SDS-PAGE revealed that the expected protein with a molecular weight of 108kD which is about 35% of the total bacterial proteins is almost soluble. The expected protein was purified directly by immobilized metal (Ni 2+) chelation affinity chromatography and the purity is over 90% . MutS protein activity verified using mismatch DNA showed that the expression product can recognize and bind to base-pair mismatch specifically.

将DNA错配修复基因mutS(2 5 6kb)克隆于分泌型原核表达载体pET32a(+)上 ,以N端融合 6个组氨酸的形式在E .coliAD494(DE3)中进行了IPTG诱导表达。SDS PAGE分析证实有一与预期分子量相应的诱导表达条带 ,其表达量占全菌蛋白质的 35 %左右 ,且表达蛋白以可溶形式存在。利用固定化金属离子 (Ni2 +)配体亲和层析柱纯化目的蛋白 ,其纯度为 90 %以上。与含有错配碱基DNA双链的结合反应证明该蛋白具有特异性识别、结合含有错配碱基DNA双链的生物活性

Accurate and fast genotyping of single nucleotide polymorphisms (SNPs) is of significant scientific importance in human genome project. Here a new automated fluorescent method that can rapidly and accurately genotype multiplex known SNPs was developed, which was achieved by substantially improving a commercially available protocol. By this method, mismatch repair gene hMLH1 and cancer repression gene P53 were investigated, eight known SNP spots (including five hot spots) of 20 coloerctal cancer patients′...

Accurate and fast genotyping of single nucleotide polymorphisms (SNPs) is of significant scientific importance in human genome project. Here a new automated fluorescent method that can rapidly and accurately genotype multiplex known SNPs was developed, which was achieved by substantially improving a commercially available protocol. By this method, mismatch repair gene hMLH1 and cancer repression gene P53 were investigated, eight known SNP spots (including five hot spots) of 20 coloerctal cancer patients′ hMLH1 and P53 gene were detected, and a heterozygosity mutation (GTT→GAT) at codon 384 of hMLH1 was found. Sequencing result of hMLH1 proves the reliability of this new method.

对人类基因组中的单核苷酸多态性进行快速而准确的定位和分型是人类基因组计划的重要内容。本文利用SNaPshot试剂盒 ,建立了一种以多重引物延伸为基础、可同时对多个已知单核苷酸多态性位点进行快速而准确的遗传分型的方法。利用这一方法检测了 2 0例大肠癌病人肿瘤组织中错配修复基因hMLH1和抑癌基因P5 3的 8个单核苷酸多态性位点 (包括 5个突变热点 ) ,其中一例发现错配修复基因hMLH1的 384位密码子处发生GTT→GAT的杂合性突变。对该基因外显子的直接测序结果与SNaPshot检测结果完全吻合 ,充分证明了该方法的可靠性。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关mismatch repair genes的内容
在知识搜索中查有关mismatch repair genes的内容
在数字搜索中查有关mismatch repair genes的内容
在概念知识元中查有关mismatch repair genes的内容
在学术趋势中查有关mismatch repair genes的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社