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restriction fragment analysis
相关语句
  限制性酶切片段分析
     The recombinant plasmid was identified by restriction fragment analysis and partial DNA sequencing. The results show that the structure of the final constructed vector accords with the designed plasmid map.
     限制性酶切片段分析及部分DNA序列鉴定结果表明,所构建载体结构正确.
短句来源
  “restriction fragment analysis”译为未确定词的双语例句
     Restriction Fragment Analysis of Chloroplast DNA from Varieties of indica and japonica Rice
     籼稻与粳稻品种间叶绿体DNA的RFLP差异
短句来源
     INTERFERING EFFECT OF DNA FROM DEGENERATING CELLS ON THE MEASUREMENT OF DNA METHYLATION ON LEVEL BY HPAⅡ RESTRICTION FRAGMENT ANALYSIS METHOD
     坏变细胞的DNA对HpaⅡ限制性片段分析法测量DNA甲基化水平的干扰作用
短句来源
     Restriction Fragment Analysis of Chloroplast DNA from Individuals of Cytoplasmic Male Sterile Lines of Rice
     水稻雄性不育品系个体间叶绿体DNA的RFLP差异
短句来源
     ResuIts: The result of restriction fragment analysis tallied with the structural map of hTR plasmid vector.
     结果:酶切鉴定的结果与hTR质粒图谱吻合。
短句来源
     Methods A total of 76 unrelated asthmatic children and 100 healthy individuals (50 children and 50 adults) were subjected from the Han nationality of Chongqing. The polymorphisms of locus 16 and 27 of the β 2 AR from all subjects were studied with PCR restriction fragment analysis.
     方法 采用PCR 限制性酶切分析法检测 76例汉族哮喘儿童 β2 AR16、2 7位多态性 ,并与 10 0例健康人进行对照 ,统计分析 β2 AR单倍型与哮喘病情严重度的关系。
短句来源
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  相似匹配句对
     Restriction Fragment Length Polymorphism in Rice
     水稻DNA限制性片段长度多态性的初步研究
短句来源
     he restriction fragment length polymorphism( RFLP ) of
     采用限制性酶切片段长度多态性分析法,检测在胃癌高发区庄河检出的19例胃癌高系家族成员胃蛋白酶原C基因多态性,发现三种常见片段及一种稀有片段。
短句来源
     The fragment was indicated specific by restriction enyme analysis.
     将此质粒进行序列分析,结果在两引物间的核酸长度为574bp。
短句来源
     Restriction Fragment Length Polymorphism Analysis of CAV Isolates from China
     鸡贫血病毒国内分离株的酶切图谱多态性分析
短句来源
     Restriction fragment Length polymorpbism analysis of bipolar mood disordcr.
     双相情感性障碍患者的基因限制性片段多态性分析
短句来源
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  restriction fragment analysis
5% (sequence analysis) to 11% (restriction fragment analysis).
      
Restriction fragment analysis of chloroplast DNA revealed no variation that could be used to discriminate between the parent species.
      
The mitochondrial genome of safflower: Isolation and restriction fragment analysis of DNA from CMS and restorer lines
      
Restriction fragment analysis of the HLA Class II region: Application to tissue transplantation
      
Restriction fragment analysis of mitochondrial DNA (mtDNA) was used to examine genetic variation and population structure in 13 species of banded-winged grasshoppers (subfamily Oedipodinae).
      
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The Hg-resistance gene from the plasmid pBH33 of Pseudomonas sp.B-33 has been cloned in E.coli C_(600) using PstI digested plasmid pBR322 as vector.The molecular weight of the recombinant plasmid pBH337 was estimated to be about 6.1 kb according to EM and gel electrophoresis.The cloned gene located on a 1.8 kb PstI fragment of the plasmid pBH33 by restriction fragment analysis of the recombinant plasmid pBH337.Re-transformation suggested that the Hg-resistance gene was located on pBH337.Determination of...

The Hg-resistance gene from the plasmid pBH33 of Pseudomonas sp.B-33 has been cloned in E.coli C_(600) using PstI digested plasmid pBR322 as vector.The molecular weight of the recombinant plasmid pBH337 was estimated to be about 6.1 kb according to EM and gel electrophoresis.The cloned gene located on a 1.8 kb PstI fragment of the plasmid pBH33 by restriction fragment analysis of the recombinant plasmid pBH337.Re-transformation suggested that the Hg-resistance gene was located on pBH337.Determination of growth showed that the recombin- ant C_(600)(pBH337)with Hg-resistance gene could grow very well in L-broth conta- ining 10ug/ml HgCl_2 and control C_(600) had strongly been repressed.The mercuric volatile of C_(600)(pBH337)was also markedly higher than that of the control C_(600)

采用pBR322为载体,将假单胞菌B-33抗汞质粒pBH33的抗汞基因,克隆至大肠杆菌C_(600)。在含25μg/ml四环素和30μg/ml HgCl_2的L—肉汤平板上生长的重组体中,筛选到一株能抗60μg/ml HgCl_2的抗汞基因克隆株——大肠杆菌C_(600)(pBH337)。重组质粒pBH337,经PstI酶解电泳分析和电镜观察表明,其分子量为6.1 kb,抗汞基因位于1.8kb长的PstI片段上。生长测定表明,克隆菌株可在含10μg/ml HgCl_2的L-肉汤中良好地生长,而受体大肠杆菌C_(600)则受到强烈抑制。汞挥发实验证明,抗汞基因克隆株C_(600)(pBH337),具有较强的去汞能力。

For the purpose of crystallography and kinetic study of N-acetyltranferase (3)-V , the encoding gene , aac (3)-Va was cloned from K.pneumonia and expressed in E. coli. The aac (3) -Va amplified from a conjugate plasmid of 85Md by PCR was ligated in pUC 18. From the transformants ,we isolated the clone encoding N-acetyltransferase (3)-V. It was proved by restriction fragments analysis and sequencing, more than 95% sequence of this gene was identical to that of S. marcescens, Most of the amino acids substitution...

For the purpose of crystallography and kinetic study of N-acetyltranferase (3)-V , the encoding gene , aac (3)-Va was cloned from K.pneumonia and expressed in E. coli. The aac (3) -Va amplified from a conjugate plasmid of 85Md by PCR was ligated in pUC 18. From the transformants ,we isolated the clone encoding N-acetyltransferase (3)-V. It was proved by restriction fragments analysis and sequencing, more than 95% sequence of this gene was identical to that of S. marcescens, Most of the amino acids substitution located in C terminal. This gene ligated with pLY4 was successfully expressed in E. coli JF1125 and DH5a.

近年的细菌耐药性监测结果发现,肺炎克雷伯菌对庆大霉素等氨基糖甙类抗生素的耐药率已达40%左右。耐药的主要机制为细菌产生N-乙酚转移酶AAC(3)-V,使其抗菌活性不能发挥。不同菌株产生的钝化酶及其编码基因有一定的差异。作者从华山医院分离的肺炎克雷伯菌耐药株,用PCR方法从该菌株的一分子量约85X10~3的接合型质粒上扩增出全长基因,连接pUC18,从转化子中筛选出编码乙酞转移酶AAC(3)-V的克隆。经限制性内切酶和DNA序列分析,并与来自粘质沙雷菌的基因比较,其核苷酸序列约有95%的同源性,20个氨基酸发生替换。大部分氨基酸的变异发生在C末端。该基因连接表达载体pLY4,转入大肠杆菌DH5α和JF1125,后两者对庆大霉素的抗性均获得大幅度提高。

Objective: To establish a method for affinity purification of human telomerase- Methods: A fragment of human telomwrase RNA (hTRcDNA) was purified and collected with electroelution after the fragment was identified with testriction enzyme digestion- The affinity chromatography beads were prepared with binding of biotinylated hTRcDNA fragment with streptavidin agarose beads- The purification was based on the ribonucleoprotein property of teIomerase with hTRcDNA cornplementary to hTR template component.After binding...

Objective: To establish a method for affinity purification of human telomerase- Methods: A fragment of human telomwrase RNA (hTRcDNA) was purified and collected with electroelution after the fragment was identified with testriction enzyme digestion- The affinity chromatography beads were prepared with binding of biotinylated hTRcDNA fragment with streptavidin agarose beads- The purification was based on the ribonucleoprotein property of teIomerase with hTRcDNA cornplementary to hTR template component.After binding reaction between the cancer cell extract with high telomerase activity and the affinity chromatography beads was set up, a complex of the affinity chromatography bead-telomerase was formed. Bound telomerase was eluted with nucleic acid denaturation reagent. TeIomerase activity was detected with telomerase PCR-ELISA. The quantity of protein and nucleic acid was assayed with Beckman DU-64o spectrophotometer. ResuIts: The result of restriction fragment analysis tallied with the structural map of hTR plasmid vector. The binding quantity and the rate of biotinylated hTRcDNA with streptavidin agarose beads were 31.71 pg and 98. 38% respectively. The affinity-purified telomerase kept active and its relative purity was lO8.46. Conclusion: Our purification method provides a feasible tool for further studies of human telomerase.

目的:建立一种亲和纯化人端粒酶的手段。方法:酶切鉴定人端粒酶RNA(hTR)质粒,所切下的hTRcDNA片段用生物素标记后与链霉亲和秦琼脂糖珠耦连,用高端粒酶活性的胃肿瘤细胞提取液与之反应,利用端粒酶核糖核蛋白的特性,形成链霉亲和秦琼脂糖珠-生物素标记hTRcDNA-hTR-端粒酶蛋白组份复合物,核酸解离液洗脱后获得亲和纯人端粒酶。其活性用宝灵曼公司端粒酶试剂盒检测,定量利用BeckmanDU-640检测仪进行。结果:酶切鉴定的结果与hTR质粒图谱吻合。生物素hTRcDNA与链霉亲和素琼脂精珠耦连量为31.71μg,耦连率为98.38%。纯化后的端粒酶活性保持良好,相对纯化程度为108.46。结论:本研究中建立的方法是一种可行的端粒酶纯化手段,可用于人端粒酶的深入研究。

 
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