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   tat gene 的翻译结果: 查询用时:0.011秒
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感染性疾病及传染病
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tat gene
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  tat基因
     Results The genetic distance respectively is 7.56% in tat gene between SC9 712 and UG274A, 9.59% and 9.94% in gag gene between SC9 712 and K31 and VI203, 13.86% in env gene between SC9 712 and MAL.
     结果发现SC9712在gag区、env区和tat区与国际标准D亚型毒株的基因距离最近,其中在gag区与V1203和K31的基因离散率分别为994%和959%,在tat基因区与UG274A的基因离散率只有756%,在env区与MAL的基因离散率是1386%。
短句来源
     Analysis of the First Exon Sequence Characteristics and Function from Tat Gene of HIV-1 Recombinant Viruses Prevalent in China
     中国HIV-1主要流行重组株B'/C Tat基因第一外显子序列特征和功能分析
短句来源
     Cloning and Characterization of TAT Gene in Tobacco and Its Homologous Gene AT103 in Arabidopsis
     烟草TAT基因和拟南芥AT103基因的克隆与功能分析
短句来源
     The recombnant HIV1 strains of subtype B and C were found in 10 of the 14 samples tested when the first exon of the tat gene was sequenced.
     对tat基因的第一外显子进行序列分析时,14个样品中的10个样品发现了B′亚型和C亚型重组的HIV1毒株。
短句来源
     Ribozymes targeted against two portions of the HIV-1 genome were designed to cleave in the tat gene (TAT) or in a common exon for tat and rev (TR).
     实验设计了两个锤头型核酶,一个针对HIV—1基因组中的tat基因(TAT—RZ),另一个针对tat基因和rev基因的共有外显子序列(TR—RZ)。
短句来源
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  “tat gene”译为未确定词的双语例句
     The fusion construction of HIV-1 Tat gene and efficient expression in E.coli
     HIV-1Tat基因的融合构建及在大肠杆菌中的高效表达
短句来源
     Conclusion:There is abnormality in the regulative domain of TAT gene or in the signal pathway of glucocorticoid receptor in SMMC-772 1cells. [
     结论 :SMMC- 772 1细胞酪氨酸转氨酶基因的调控序列或GR的信号转导通路存在异常
短句来源
     Methods 93 DNA fragments of HIV-1 env, gag, tat gene were amplified by nested PCR from those infected with HIV-1, in 2002-2003. Their C2-V3, P17/P24, 1st exon of tat and adjacent region were sequenced.
     方法采集93份HIV-1感染者的外周静脉防凝血,提取前病毒DNA进行体外扩增,获得包膜蛋白(env)、核心蛋白(gag)、tat区基因的核酸片段,并对各基因区核苷酸进行测定和分析。
短句来源
     This study is to explore whether the variation of tat gene influences the AIDS progress of HIV infectect individual.
     本研究是探讨tat基因变异是否影响HIV-1感染者的病程进展。
短句来源
     INHIBITING OF SYNTHESIS OF HIV PROTEIN USING ANTI-SENSE TAT GENE CARRIED BY
     MS2噬菌体壳蛋白载动的HIV反链基因阻断病毒蛋白质合成
短句来源
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  相似匹配句对
     This result confirm that mutation was occur independly in tat gene.
     这初步确定了这些突变菌株为 Tat 转运系统的相关基因发生突变,而非 tat 基因。
短句来源
     -casein gene.
     -酪蛋白基因5'侧序列。
短句来源
     On Gene Diagnosis
     略论基因诊断
短句来源
     Cloning and Characterization of TAT Gene in Tobacco and Its Homologous Gene AT103 in Arabidopsis
     烟草TAT基因和拟南芥AT103基因的克隆与功能分析
短句来源
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  tat gene
Promoter elements in the U3 region of the 3' LTR of the vector pLXSN were deleted and replaced with DNA encoding the HIV anti-tat gene under control of the tRNAmet promoter to produce a double copy self inactivating vector (DC-SIN).
      
The human immunodeficiency virus (HIV-1) encodes a transactivator protein, the product of the tat gene (tat), which is essential for virus replication.
      
Although the Tat protein of the primate lentiviruses is essential for virus replication, ORF A (putative Tat gene) of FIV is not essential for virus replication in established feline T lymphoblastoid cell lines.
      
The activities of the novel tat gene translational products were assayed by measuring the cotransfected chloramphenicol acetyl-transferase (CAT) gene expression controlled by HIV long-terminal repeat (LTR) in the COS 7 cells.
      
The role of this region was investigated by creation of several tat gene mutants.
      
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The Tat trans-activators encoded by the primate immunodeficiency virus(HIV-1,HIV-2,and SIV) share three highly conserved domains:Cys-rich domain,core region and basic domain.Site-directed mutagenesis by PCR method of these regions in the Tat gene of human immunodeficiency virus type 1 is shown to greatly reduce or abolish the activity of Tat protein when PM1-6 was cotransfected transiently with an expression vector that the reporter gene for luciferase(Luc) was driven by HIV-1 LTR(from-158...

The Tat trans-activators encoded by the primate immunodeficiency virus(HIV-1,HIV-2,and SIV) share three highly conserved domains:Cys-rich domain,core region and basic domain.Site-directed mutagenesis by PCR method of these regions in the Tat gene of human immunodeficiency virus type 1 is shown to greatly reduce or abolish the activity of Tat protein when PM1-6 was cotransfected transiently with an expression vector that the reporter gene for luciferase(Luc) was driven by HIV-1 LTR(from-158 to+80) promoter.The wild-type and mutant Tat gene were also directed by HIV-1 LTR(-158 to +80 fragment).The results demonstrated that the precise sequence of these conserved regions are crucial for the function of Tat protein.

不同免疫缺陷病毒(HIV-1,HIV-2和SIV)的Tat均有3个高度保守的结构域:Cys富集域、核心域和碱性氨基酸富集域,用PCR定点突变法在HIV-1Tat蛋白的这些区域引入单氨基酸或多氨基酸突变;构建了以HIV-1LTR-158到+8O区域为启动子,含有不同突变点的突变Tat基因表达质粒;以荧光酶基因为报告基因,瞬时共转染Jurkat细胞:分析不同氨基酸突变对Tat的反式激活作用的影响。结果发现,突变后的Tat蛋白的活性均极大地降低或丧失,表明这些序列内的氨基酸的确定性对Tat的活性至关重要。

Objective HIV 1 tat gene is one of its regulatory genes necessary for its riplication. This study is to explore whether the variation of tat gene influences the AIDS progress of HIV infectect individual. Methods We carried out investigation on tat gene of HIV among the long term HIV infected non progressors and the commonly infected individuals. Mononuclear cells of peripheral blood were isolated and cellular DNA was extracted. Nested PCR method was used to amplify tat gene...

Objective HIV 1 tat gene is one of its regulatory genes necessary for its riplication. This study is to explore whether the variation of tat gene influences the AIDS progress of HIV infectect individual. Methods We carried out investigation on tat gene of HIV among the long term HIV infected non progressors and the commonly infected individuals. Mononuclear cells of peripheral blood were isolated and cellular DNA was extracted. Nested PCR method was used to amplify tat gene and their sequences were analyzed. Results Blood were collected from 22 HIV infected individuals, of which 11 cases were infected with B subtype strains based on previous env gene analysis and belonged to long term non progressors according to clinical features and immunology. The other 11 cases were common HIV infection of which 5 subtype B and 6 subtype C were found. Both long term non progressor group (subtype B) and the commonly HIV infectedly group (subtype B) had constantly four amino acids different from that of international B subtype consensus sequence The both groups clusteed randomly on the phylogenetic tree. In commonly HIV infected subtype C group there were 6 amino acids different from that of international subtype C. The variation inside the tatgene of common subtype C group was small.Conclusions There was no regular variation and significant difference between long term HIV infected non progressve group and commonly HIV infected subtype B group. There is no evidence showing that progressive speed of AIDS correlated with the tat sequence profile of HIV 1.

目的人类免疫缺陷病毒1型(HIV-1)tat基因是该病毒的调控基因之一。本研究是探讨tat基因变异是否影响HIV-1感染者的病程进展。方法从云南HIV流行区的22例HIV感染后临床进程不同的人抽取外周血,提取核酸,用套式聚合酶链反应(PCR)扩增HIV-1的tat基因,并进行了核酸序列测定和分析。结果22个HIV毒株中,有11株来自长期不进展者,均为B亚型。另外11株来自一般感染者的HIV毒株,5株为B亚型,6株为C亚型。长期不进展者和HIV-1B亚型一般感染者的tat基因序列与国际B亚型共享序列比较,两组均在相同的固定的4个位点上氨基酸与共享序列不一致。在系统树分析中两组亦聚集在一起,未显示出分离。C亚型一般感染者的tat基因序例组内变异小,在固定的6个位点上与国际C亚型共享序列不一致。结论长期不进展者与一般B亚型感染者的Tat蛋白序例均未发现规律性差异。HIV感染后的进展速率可能与HIV-1tat基因序例无明显关系。

Objective To search for possible recombination of HIV1 strains in China. Methods The blood samples were collected from the HIV infected individuals in areas where epidemic of more than two subtypes of HIV1 were found. The HIV env and tat genes were amplified by nested PCR from PBMCs and directly sequenced and analyzed. Results The sequence analysis were performed on 14 HIV1 strains collected in the subtype Band C epidemic areas of China. No evidence of recombination after sequencing of env was found....

Objective To search for possible recombination of HIV1 strains in China. Methods The blood samples were collected from the HIV infected individuals in areas where epidemic of more than two subtypes of HIV1 were found. The HIV env and tat genes were amplified by nested PCR from PBMCs and directly sequenced and analyzed. Results The sequence analysis were performed on 14 HIV1 strains collected in the subtype Band C epidemic areas of China. No evidence of recombination after sequencing of env was found. The recombnant HIV1 strains of subtype B and C were found in 10 of the 14 samples tested when the first exon of the tat gene was sequenced. In addition, three subtype B and one subtype C nonrecombinant HIV1 strains were found among IDUs in Sichuan Province. Conclusions The recombinant HIV1 strains of subtype B and C were firstly identified in Sichuan Province of southwest China and Xinjiang Autonomous Region of western China. Their identical sequences and recombinant patterns indicated the same origin of the recombinant strains, it strongly indicates a close correlation between these two HIV epidemic regions. Since no other subtype of HIV1 except the recombinant was found in Xinjiang, whereas both subtype B and C as well as B/C recombinant were found in Sichuan, the recombination was most probably happened in Sichuan Province rather than in Xinjiang.

目的寻找人类免疫缺陷病毒1型(HIV1型)在中国可能的重组。方法从流行2种以上HIV1亚型的地区收集HIV感染者的血样。从PMCs中应用套式聚合酶链反应(PCR)方法,对HIV病毒的tat和env基因进行扩增,PCR产物直接测序并进行序列分析。结果对中国B′亚型和C亚型流行区域收集的14个HIV1毒株进行序列分析,对env基因进行测序后没有发现重组毒株的证据。对tat基因的第一外显子进行序列分析时,14个样品中的10个样品发现了B′亚型和C亚型重组的HIV1毒株。此外,四川省静脉吸毒者中发现了3个非重组的B′亚型毒株和1个非重组的C亚型毒株。结论首次在中国西南部的四川省和西部的新疆维吾尔自治区发现了B′亚型和C型的重组HIV1毒株。相同的序列和重组方式,表明重组毒株具有相同的起源,同时表明这两个艾滋病流行区域密切相关。由于在新疆只发现了重组毒株,而在四川省则发现了B亚型、C亚型和B′/C重组毒株,重组很有可能发生在四川而不是新疆。

 
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