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upstream regulation
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  “upstream regulation”译为未确定词的双语例句
     Identification of 5-flank upstream regulation region of CD226
     CD226基因5′上游调控序列研究
短句来源
     Gel mobility shift assay also confirmed BB0462 protein only bound to oppAⅤ upstream regulation DNA fragment.
     用未标记的oppAⅤ上游调控区DNA与DIG-标记的oppAⅤ上游调控区DNA竞争BB0462蛋白,使凝胶上的DNA迁移阻滞带完全消失.
短句来源
     Preliminary study of relationship between upstream regulation sequence of Candida albicans CDR1 gene and fluconazole resistance
     白念珠菌CDR1基因上游调控序列与氟康唑耐药的关系初探
短句来源
     It's found that the role of the upstream regulation regions which were at -1.3kb to -1 and at -7.2 kb to -1 was possibly the same.
     -7.2 kb范围内与-1.3kb范围内的远端调控序列在细胞水平上对目的蛋白的表达差别不大。
短句来源
     Cloning of 5′Upstream Regulation Sequence for a High Molecular Weight Glutenin Gene in Wheat Store Protein
     小麦贮藏蛋白中高分子量谷蛋白基因的5′上游调控序列克隆
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     Study on the engineering of channel regulation in upstream Hanjiang River
     韩江上游(梅江、汀江)航道整治工程试验研究
短句来源
     transmission regulation;
     输电监管;
短句来源
     Regulation of Baculovirus
     昆虫杆状病毒基因表达的调控
短句来源
     METHODS: The upstream regulation sequences of amelogenin gene were retrieved and analyzed.
     方法:检索并分析釉原蛋白基因的上游调控序列。
短句来源
     support upstream interaction;
     支持上行交互;
短句来源
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  upstream regulation
However, almost nothing is known about the upstream regulation of the senescence specific expression of WRKY factors.
      
This review summarises recent progress that has been made in understanding the molecular links between the core fusion machinery and upstream regulation.
      
Our results indicate that upstream regulation via Cx43 controls the Shh and Bmp-2 pathways for the morphogenesis and pattern formation of fungiform papillae.
      
Analysis of DNA retardation and competitive repression also confirmed that the BB0462 protein bound to the 409 bp upstream regulation DNA fragment close to the initiation codon of the oppAV gene.
      
The co-transformation of BB0462 ORF and oppA upstream regulation DNA into E.
      
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Genes encoding high molecular weight(HMW)glutenin, a wheat seed storage protein, are expressed only in the developing endosperm. It was previously subclones of a wheat ( Triticum aestivum L .cv. asarce) 2.8kb DNA that contained 0.4kb of HMW glutenin gene sequence and 2.4 kb of upstream regulation sequence. With the site specfic mutagensis method,a novel restriction enzyme site BamHⅠ is obtained by changing the three bases between TATA box and initiation codon ATG, and a series of truncated HMW...

Genes encoding high molecular weight(HMW)glutenin, a wheat seed storage protein, are expressed only in the developing endosperm. It was previously subclones of a wheat ( Triticum aestivum L .cv. asarce) 2.8kb DNA that contained 0.4kb of HMW glutenin gene sequence and 2.4 kb of upstream regulation sequence. With the site specfic mutagensis method,a novel restriction enzyme site BamHⅠ is obtained by changing the three bases between TATA box and initiation codon ATG, and a series of truncated HMW glutenin gene promoters which are respectly 2400, 610, 440 and 110bp DNA fragments. Four fragments are inserted into plasmid pBI101.1 respectively and four plasmids of pWG2400, pWG600, pWG440 and pWG100 are constructed. The plasmid pWG2400 is transferred into the endosperm cells of Zea mays by means of particle gun,laser microbean and PEG, and the GUS gene is expressed in transgenic cells. These plasmids provide valuable materials for further analysis of HMW glutenin gene expression and regulation.

通过定点突变,在小麦高分子量(HMW)谷蛋白基因5′上游调控序列的TATAbox与基因翻译起始密码子ATG之间,改变三个碱基,产生新的BamHⅠ内切酶位点.在调控序列中,选择四个合适的内切酶,对上游序列进行缺失,分离到四个大小分别为2400、610、440和110bp的含有不同调控元件的HMW谷蛋白基因启动子,与质粒pBI101.1的报告基因GUS重组,构建了四个嵌合质粒pWG2400、pWG600、pWG440和pWG100.经基因枪、微束激光和PEG等方法将pWG2400转化玉米胚乳悬浮细胞,GUS基因获得表达.这为进一步研究HMW谷蛋白基因的调控机理奠定了基础.

Apoptosis was observed in P 75NGFR expressing R2 cells (R2L1)after depriving serum from the culture medium,but not in PXT 1 transfected control R2 cells (R2P).Differential display technique was used to reveal the differentially expressed genes between normal control and apoptotic neural cells.Several short cDNA fragments were cloned and sequenced.These differentially expressed fragments were further confirmed by Northern blotting hybridization with their original total RNAs.The result showed that there...

Apoptosis was observed in P 75NGFR expressing R2 cells (R2L1)after depriving serum from the culture medium,but not in PXT 1 transfected control R2 cells (R2P).Differential display technique was used to reveal the differentially expressed genes between normal control and apoptotic neural cells.Several short cDNA fragments were cloned and sequenced.These differentially expressed fragments were further confirmed by Northern blotting hybridization with their original total RNAs.The result showed that there were two of the short cDNA fragments LIAREST 1 and LIAREST 2 whose expression levels in apoptotic serum depriving cultured R2L1 cells were significantly higher than in normal serum containing cultured R2L1 cells and serum depriving cultured R2P cells.Sequence analysis showed that both of them contained a 3'poly A tail and a polyA tail adding signal in the 17 nucleotides upstream from 5' ending of polyA.Another short cDNA fragment LIARCD 3 was observed,whose expression level in apoptotic serum depriving cultured R2L1 cells was 63 4% and 62 3% lower than in serum containing cultured R2L1 cells and serum depriving cultured R2P cells,respectively.The full length of this cDNA fragment was cloned by Gu Jun of NIH in 1994 and is accessible with the accession number U08214.It encodes a protein(UreB 1)binding to an 8 nucleotide upstream regulation element(URE)for a translation initator element in the preprodynorphin promoter,and with a tyrosine kinase phosphorylation consensus.The role of these genes in apoptosis of neural cells in unknown at present.

转染了P75NGFR的R2神经细胞系R2L1在去血清的培养时可以诱导细胞凋亡的发生.此凋亡可以被RNA合成抑制剂放线菌素D和蛋白质合成抑制剂环己酰胺所抑制.利用DDRT-PCR技术比较了去血清培养的发生凋亡的R2L1细胞与有血清培养的不发生凋亡的R2L1细胞以及去血清培养的不发生凋亡的R2P细胞基因表达的差异.克隆了数个特异或差异表达的短cDNA片段,经Northern杂交证实其中两个片段LIAREST-1和LIAREST-2表达量在凋亡细胞中显著高于不发生凋亡的细胞中,GenBank检索表明此二片段为新的cDNA序列并给予登录号U47315和U47316.另有一个cDNA片段LIARCD-3在凋亡细胞中受到了明显的抑制,经检索为一已知的与前强啡肽原上游调控区结合的DNA结合蛋白cDNA编码区的一部分,首次被证实它与P75NGFR诱导的神经细胞凋亡调控关联

The analysis of special hydraulic condition and operation problem of batching in pipelines is presented. The general energy balance equation of pipeline and the synthetic dynamic pressure e-quation of the critical points in pipeline are proposed. The method of seeking the allowable maximum throughput, which is subjected to the operational condition, is also proposed. With the throughput,the surplus of pumping pressure for the flow dissipation are calculated,which must be suppressed by regulating. In accordance...

The analysis of special hydraulic condition and operation problem of batching in pipelines is presented. The general energy balance equation of pipeline and the synthetic dynamic pressure e-quation of the critical points in pipeline are proposed. The method of seeking the allowable maximum throughput, which is subjected to the operational condition, is also proposed. With the throughput,the surplus of pumping pressure for the flow dissipation are calculated,which must be suppressed by regulating. In accordance with the principle of upstream regulation priority,the regulating pressure is reasonably apportioned to pumping stations to acquire the benefit of making pressure gradient as near as possible to pipeline profile. In the end,the general computational scheme for the optimal operation of batching in pipelines is offered.

分析了管道顺序输送水力状态及工况调节的特殊性,给出了表述油品“界面”处于管道各特殊点时全线能量平衡方程和各校核点动压的通用计算式。以寻求输量最大为目标,阐述了满足工艺约束的最大可行输量的搜索解算方法,给出了解算最优工况的流程图。

 
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