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   轻链基因 的翻译结果: 查询用时:0.047秒
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轻链基因
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  light chain genes
     The chimeric Fab expression vector was constructed by cloning the chimeric heavy chain FD and light chain genes into pComb3.Fab antibodies were expressed on phage by superinfection with the helper phage VCSM13,and the binding activity of antibody was detected by indirect ELISA and competitive inhibition ELISA.
     将VH基因与人重链恒定区CH1基因连接,VL基因与人CK基因连接,分别构建了鼠/人嵌合重链FD基因和轻链基因,将其克隆入pComb3,构建了鼠/人嵌合Fab噬菌体抗体表达载体,用辅助噬菌体VCSM13超感染,间接ELISA及竞争抑制ELISA检测鼠/人嵌合Fab噬菌体抗体活性。
短句来源
     Cloning and Identification of Fd and Light Chain Genes of MAb HAb18 against Human Hepatoma
     抗人肝癌单克隆抗体HAb18 Fd及轻链基因的克隆与鉴定
短句来源
     Aim:To clone Fd and light chain genes and V_H and V_L genes of monoclonal antibody OX34 against human CD2 and verify their accuracy and liability.
     目的:本实验设计引物分别克隆抗人CD2单抗OX34 Fd和轻链基因以及轻、重链可变区基因V_H和V_L,然后将OX34 Fd和轻链基因克隆入噬菌体展示载体pComb3;
短句来源
     METHODS Mouse/human chimeric heavy chain Fd (Fd fragment) and light chain genes were generated by ligation of V H gene of mouse mAb 1A8 against HFRS virus with human heavy chain CH1 gene and V L gene with human C κ gene, respectively.
     方法 将抗 HFRS病毒m Ab1 A8的 VH 基因和人重链 CH1基因连接 ,VL 基因和人Cκ 基因连接 ,分别构建了鼠 /人嵌合重链 Fd基因和轻链基因 ,将它们克隆入 p Comb3,构建了鼠 /人嵌合 Fab噬菌体抗体表达载体 .
短句来源
     Methods The chimeric Fd and light chain genes were inserted into prokaryotic expression vector pET32a to construct recombinant vectors pET32a/cFd and pET32a/cL. Then, recombinant vectors were transformed into E. coli and induced by IPTG.
     方法 分别将嵌合Fd及嵌合轻链基因插入到原核表达载体pET32a中 ,构建成重组载体pET32a/cFd和pET32a/cL。
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  light chain gene
     Expression of light chain gene of human anti-HFRS virus antibody in Sp2/0cells
     人源性抗HFRS病毒抗体轻链基因在Sp2/0细胞中的初步表达
短句来源
     Cloning and sequence analysis of light chain gene of human antibody against HBV pre-S2
     人抗HBV-前S2抗体轻链基因的克隆及序列分析
短句来源
     Sequence and analysis of human anti-HBsκ light chain gene
     人抗HBs轻链基因的序列测定及序列分析
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     Expression of Protein Encoded by λ Light Chain Gene of Bursal B Lymphocytes in E.coli
     法氏囊B细胞λ轻链基因编码蛋白在大肠杆菌中的表达
短句来源
     Nuclear transcription factor κB (NF-κB) was firstly found by Ranjan Sen etc. in the B lymphocyte in 1986, which was one of the most important transcription factors and named from the special binding with the enhancer κB sequence of immunoglobulin kappa light chain gene.
     核转录因子NF-κB (nuclear factor κB)是由Ranjan Sen等人于1986年首先在B 淋巴细胞中发现,得名于它能够与B细胞免疫球蛋白κ轻链基因的增强子κB序列特异地结合,是近年来发现的最重要的转录因子之一。
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  chain gene
     Expression of light chain gene of human anti-HFRS virus antibody in Sp2/0cells
     人源性抗HFRS病毒抗体轻链基因在Sp2/0细胞中的初步表达
短句来源
     Cloning and sequence analysis of light chain gene of human antibody against HBV pre-S2
     人抗HBV-前S2抗体轻链基因的克隆及序列分析
短句来源
     Sequence and analysis of human anti-HBsκ light chain gene
     人抗HBs轻链基因的序列测定及序列分析
短句来源
     Expression of Protein Encoded by λ Light Chain Gene of Bursal B Lymphocytes in E.coli
     法氏囊B细胞λ轻链基因编码蛋白在大肠杆菌中的表达
短句来源
     Nuclear transcription factor κB (NF-κB) was firstly found by Ranjan Sen etc. in the B lymphocyte in 1986, which was one of the most important transcription factors and named from the special binding with the enhancer κB sequence of immunoglobulin kappa light chain gene.
     核转录因子NF-κB (nuclear factor κB)是由Ranjan Sen等人于1986年首先在B 淋巴细胞中发现,得名于它能够与B细胞免疫球蛋白κ轻链基因的增强子κB序列特异地结合,是近年来发现的最重要的转录因子之一。
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  “轻链基因”译为未确定词的双语例句
     Sequences comparison of the corresponding exon region from 4 different Bombyx mori varieties showed that nucleic acid homology was between 98.0%~99.4%, the deduced amino acid homology was between 98.0%~99.6%, and the base variation was focused on 8 different sites.
     比较 4种不同品种来源的家蚕丝心蛋白轻链基因外显子区域序列 ,同源性在98.0 %~ 99.4 %之间 ,推测的氨基酸同源性在 98.0 %~ 99.6 %之间 ,4个品种间发生的变异比较一致地集中在不同位置的 8个碱基上 .
短句来源
     2. VH and VL genes of mAb E10 were ligated with human heavy chain CH1 and human CK genes.
     2. 将VH基因与人重链恒定区CH1基因连接,VL基因与人CK连接,分别构建了鼠/人嵌合重链FD基因和轻链基因
短句来源
     Methods We constructed a recombinant eukaryotic expression plasmid,pcDNA3.0-Igκ-hCT,which contains human calcitonin gene and Murine Igκ-chain leader sequence.
     方法采用自行构建的5'端融合小鼠抗体轻链基因Igκ的信号肽序列的人降钙素基因分泌性真核表达载体pcDNA3.0-Igκ-hCT,采用脂质体介导方法将人降钙素基因导入大鼠成肌细胞;
短句来源
     Based on the sequences alignment of other fishes, degenerate primers of grass carp MHC I light chain were designated to amplify Ctid-β_2m. 5' from the library, and then Ctid-β_2m full cDNA was cloned by anchored-PCR.
     根据动物MHC Ⅰ轻链基因(β_2m)序列设计了兼并引物,从文库中克隆了Ctid-β_2m 5′-端cDNA,再用Anchored-PCR克隆了Ctid-β_2m全基因。
短句来源
     Methods:The genes of light chain and Fd fregment encoding 140 Fab antibody were inserted into p3MH vector to construct a recombinant prokaryotic expression vector p3MH/P140к-Fd,and it was transformed into E.
     方法:从含有抗血小板抗体轻链基因和重链Fd段基因的克隆载体pGEM T-Easy上经双酶切获得抗体轻链基因和重链Fd段基因片段,将其以特定位点分别插入噬菌体抗体表达载体p3MH上,构建原核表达重组质粒p3MH/P140к-Fd。 转化E.
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  light chain genes
When applied to the heavy and light chain genes of our model antibody, we found that greater numbers of high-producing transfectants as well as increased levels of protein production were observed (≈1.5-fold).
      
Linkage of a 7S RNA sequence and kappa light chain genes in the mouse
      
The human immunoglobulin lambda-like (IGLL) genes, which are homologous to the human immunoglobulin lambda (IGL) light chain genes, are expressed only in pre-B cells and are involved in B cell development.
      
We have developed a simple, rapid, and reproducible PCR-based technique to clone any rearranged mouse Ig heavy or light chain genes.
      
Transcriptional enhancers of immunoglobulin light chain genes in Atlantic cod (Gadus morhua?)
      
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  light chain gene
Based on these results, we propose a novel scheme for interactions between NF-κB p50 homodimer and the κB region of the immunoglobulin light chain gene enhancer (Ig-κB).
      
We have also demonstrated that activation of NF-κB signaling in precursor B cells is required for global regulation of Ig light chain gene assembly.
      
Identification of two novel mutations in the ventricular regulatory myosin light chain gene (MYL2) associated with familial and
      
The lambda light chain locus classically contains two V and four J-C gene segments in inbred mouse strains, and was physically mapped in the BALB/c cell line Wehi-3 which contains unrearranged lambda light chain gene segments.
      
Mapping and nucleotide sequence of a newHLA class II light chain gene,DQB3
      
更多          
  chain gene
Based on these results, we propose a novel scheme for interactions between NF-κB p50 homodimer and the κB region of the immunoglobulin light chain gene enhancer (Ig-κB).
      
Decreased expression of the human immunoglobulin J-chain gene in squamous cell cancer and adenocarcinoma of the lungs
      
PDGF-B chain gene is 92% homologous to v-sis oncogene of the simian sarcoma virus.
      
Cloning, sequencing and expression of a swine IgG H chain gene inE.
      
The DNA fragment encodes immunoglobulin IgG H chain gene.
      
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Using a newly developed method,the

我采用点杂交的方法,对人β型血珠蛋白基因簇的染色质结构与基因转录活性之间的关系进行了研究。以对DNase Ⅰ消化的敏感性作为染色质的结构参数,我将β型血珠蛋白基因簇中11个区域之间以及其与不表达基因区(乳糖白蛋白和免疫球蛋白不变区λ轻链基因)的染色结构进行比较。实验的细胞系统为K 562红白血病细胞与人胚皮肤细胞株(HES)。所获得的结果提示,在细胞核内,表达基因的染色质结构疏松,对DNase Ⅰ消化的敏感性远较不表达基因区的为高。此外,本文还对有关点杂交的方法学问题进行了较为详尽的讨论。

A monoclenal antibody CL-3has been characterized as displaylng a

小鼠抗结肠癌单抗CL-3经体内、外研究证实它对结肠癌细胞具有很高的特异性。为了使该单抗能够应用于肿瘤导向治疗,我们采用基因工程技术,从CL-3杂交瘤细胞系中克隆出了CL-3单抗κ轻链基因,并对该基因的全部核苷酸序列进行了测定。

The B-cell clonality of 22 cases of non-Hodgkin's lymphoma was analysed by using the immunoglobulin JH, CK and C_λ gene probes and Southern blot hybridization method.All the 13 cases of B-cell lymphoma, which were confirmed by histopathology and immunohistochemistry, showed the non-germlime rearrangement of Ig heavy chain gene, 10 of them being with k or λ light gene rearrangement, whereas one of the three T-cell lymphomas with Ig heavy chain gene and another one with k light chain gene rearrangement. One case...

The B-cell clonality of 22 cases of non-Hodgkin's lymphoma was analysed by using the immunoglobulin JH, CK and C_λ gene probes and Southern blot hybridization method.All the 13 cases of B-cell lymphoma, which were confirmed by histopathology and immunohistochemistry, showed the non-germlime rearrangement of Ig heavy chain gene, 10 of them being with k or λ light gene rearrangement, whereas one of the three T-cell lymphomas with Ig heavy chain gene and another one with k light chain gene rearrangement. One case of undetermined lymphoma was with heavy chain gene rearrangement. In the other 5 cases of lymphoma, which were without immunophenotyping, there were 4 heavy chain, 3 with k light chain and one with λ chain gene rearrangements. The results suggest that Ig gene rearrangement, especially when the heavy and light chain gene rearrangements are found in the same cae, is the specific molecular biological marker of B-cell lymphoma.

用免疫球蛋白重链J区(JH),K轻链及λ轻链C区(Ck、Cλ_2)基因探针及Southern印迹杂交技术,对22例非何杰金氏淋巴瘤进行分析。结果显示13例B细胞淋巴瘤均出现重链基因重排,其中10例行K或λ轻链基因重排;3例T细胞淋巴瘤中,1例出现重链,每1例出现K轻链基因重排。1例分类未明淋巴瘤出现重链基因重排。5例未作免疫表型分析的淋巴瘤中有4例出现重链,3例出现K轻涟,1例出现λ轻链基因重排。对免疫球蛋白基因重排分析在淋巴瘤诊断中的意义进行了讨论。

 
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