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adaptive culture
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  “adaptive culture”译为未确定词的双语例句
     Selection of Adaptive Culture Medium for Swe Stem Apexetpotato
     甘薯茎尖培养的适宜培养基筛选
短句来源
     The French bread yeast(Saccharomyces ceyevlsiae) with comparatively high productivity and zinc content was subjected to adaptive culture under high zinc concentration. After ninety generations'culture of the yeast, its zinc content was 4000~15000μg/g.
     选择法国面包酵母为菌种,在高锌条件下进行90代的适应性培养,获得了具有较高产率的富锌酵母株,含锌量4000~15000μ/g。
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  相似匹配句对
     Culture
     文化艺术
短句来源
     t for C. t culture.
     建立C.
短句来源
     Selection of Adaptive Culture Medium for Swe Stem Apexetpotato
     甘薯茎尖培养的适宜培养基筛选
短句来源
     Adaptive Networking
     适应性网络
短句来源
     the system has to be adaptive.
     系统必须是自适应的。
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  adaptive culture
The lesson learned in these examples is the need for a flexible environment and an adaptive culture.
      


The French bread yeast(Saccharomyces ceyevlsiae) with comparatively high productivity and zinc content was subjected to adaptive culture under high zinc concentration. After ninety generations'culture of the yeast, its zinc content was 4000~15000μg/g.Its growth conditions was researched, including concentration of inorganic zinc, amount of inoculation, pH, carbon and nitrogen sources. Its acute toxicity in mice was also investigated.

选择法国面包酵母为菌种,在高锌条件下进行90代的适应性培养,获得了具有较高产率的富锌酵母株,含锌量4000~15000μ/g。对该株酵母的最适生长条件(无机锌浓度、菌种接种量、酸碱度、碳源、氮源)及小鼠急性毒性进行了研究。

Thiobacillus ferrooxidans oxidating ferrous sulphate effectively at low pH (pH<2.0) is prerequisite to the industrial application of two-step bioleaching. In this work, the strain was adapted by sequential adaptive culture and isolation via continuous culture. Compared with the original Thiobacillus ferrooxidans TF5, its optimum pH for oxidating ferrous iron was changed from 2.0~3.0 to 1.7~2.0, and the ratio of oxidized ferrous iron was improved from 13.1% to 85% at pH=1.7. Kinetic research showed...

Thiobacillus ferrooxidans oxidating ferrous sulphate effectively at low pH (pH<2.0) is prerequisite to the industrial application of two-step bioleaching. In this work, the strain was adapted by sequential adaptive culture and isolation via continuous culture. Compared with the original Thiobacillus ferrooxidans TF5, its optimum pH for oxidating ferrous iron was changed from 2.0~3.0 to 1.7~2.0, and the ratio of oxidized ferrous iron was improved from 13.1% to 85% at pH=1.7. Kinetic research showed that, under the optimized cultural condition of initial pH=1.7, culture temperature near 31oC, 98% of ferrous iron contained in 9K medium was oxidized after cultivation for 52 h in a 250 ml Erlenmeyer flask incubated on a rotary shaker at 120 r/min, and the growth rate of Thiobacillus ferrooxidans cells was 0.0635 h-1, which is similar to the original type. It was showed that the adapted Thiobacillus ferrooxidans is potentially useful in the industrial application of two-step bioleaching.

低pH条件下高效氧化硫酸亚铁的氧化亚铁硫杆菌的选育是两步法生物浸出工艺应用的前提条件. 本文经定向培育和连续培养筛选得到pH为1.7~2.0条件下氧化硫酸亚铁的氧化亚铁硫杆菌驯化菌,其氧化硫酸亚铁的最适pH值由原来的2.0~3.0下降到1.7~2.0,在初始pH为1.7条件下培养48 h后,9K培养基中Fe2+氧化率从13.1%提高到85%. 动力学研究结果表明,该菌在250 ml摇瓶中装量100 ml、接种量10%、起始pH=1.7、温度31oC、转速120 r/min的优化条件下培养52 h后,9K培养基中Fe2+氧化率在98%以上,对数期的细菌比生长速率为0.0635 h-1,与出发菌株相近. 该菌有望用于两步法生物浸出矿物的新工艺.

The characteristics of a cultured cell line do not always remain stable and may change upon continuous passage. Most continuous cell lines, even after cloning, possess several genotypes that are constantly changing. There are numerous selective and adaptive culture processes, in addition to genetic instability, that may improve phenotypic change in cell growth, virus susceptibility, gene expression, and production of virus. Similar detrimental effects of long term passaging of insect cells have also been...

The characteristics of a cultured cell line do not always remain stable and may change upon continuous passage. Most continuous cell lines, even after cloning, possess several genotypes that are constantly changing. There are numerous selective and adaptive culture processes, in addition to genetic instability, that may improve phenotypic change in cell growth, virus susceptibility, gene expression, and production of virus. Similar detrimental effects of long term passaging of insect cells have also been reported for continuous cell lines, for example, Tn5B1-4 cells, which are the most widely used for the baculovirus expression vector system (BEVS), provide superior production of recombinant proteins, however, this high productivity may be more evident in low passage cells. In this paper, we describe the isolation of a cell clone, Tn5B-40, from low passage Tn5B1-4 cells. The growth characteristics, productions of virus, and high level of recombinant protein productions were determined. The results showed the susceptibility of both clone and Tn5B1-4 cells to wild-type AcNPV was approximately the same rate with over 95% of infection; when the cloned cells were infected with recombinant baculoviruses expressing β-galactosidase and secreted alkaline phosphatase (SEAP), expression of the recombinant proteins from the cloned cells exceeded that from the parental Tn5B1-4 cells.

从Tn5B1-4细胞系中克隆并筛选出了新克隆株Tn5B-40,测定分析结果表明,该克隆株对病毒(AcNPV)的敏感性和生长特性与原始细胞无显著差异,但在重组蛋白表达方面,无论对β-半乳糖苷酶还是碱性磷酸酶都明显地高于野生型细胞系,其中β-半乳糖苷酶在第6天的表达为原始细胞株的2倍,碱性磷酸酶的表达在第9天最高为原始细胞株的1.4倍。因此,该细胞是一株高产病毒和高表达重组蛋白的新克隆株。

 
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