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constant region
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  恒定区
     Cloning for cDNA of constant region CH_2-CH_4 on IgE heavy chain of Han nationality in China
     中国汉族人IgE重链恒定区CH_2-CH_4cDNA的克隆
短句来源
     The relative atomic percent content(AC%) of the film obtained from the elemental composition constant region of the profile curves by AES was Fe 17. 1%,Mo 13. 5%,Si 8. 8%, O 57.4% and C 3.2% respectively.
     从AES深度分布曲线的组成恒定区求得膜层的组成(A.C.%)为:O 57.4%,Fe 17.1%,Mo 13.5%,Si 8.8%,C 3.2%
短句来源
     The relative atomic percent contents(A. C. %) of the film, as obtained from the element composition constant region of the profile curves, were 48.4%O; 28.6%P;
     在元素组成近似恒定区测得膜的相对原子浓度(A.C.%)为:O48.4%P28.6%;
短句来源
     A mouse-human 6C6 chimeric antibody gene, which is a linkage of variable region gene of mouse 6C6 monoclone antibody and constant region gene of human antibody, has been cloned and effectively expressed in CHO cells.
     采用基因工程技术 ,将小鼠 6C6单克隆抗体可变区基因与人抗体恒定区基因连接 ,构建了鼠 人 6C6嵌合抗体基因 ,并在CHO细胞中高效表达 .
短句来源
     Using these cDNA fragments as probes, the L and H chain V region exons encoding the murine McAb anti-CD3 were isolated from the gene library of HIT3a DNA and inserted into mammalian expression vectors containing the human κ and yl constant region exons for construction of human/murine chimeric antibody genes.
     以此轻重链可变区cDNA为特异探针从HIT3a基因文库中分离带有调控序列的功能性轻重链可变区基因; 并将其插入到含有人κ轻链及人γ1重链恒定区基因的哺乳动物表达载体中成功地构建了抗人CD3人/鼠轻重链嵌合抗体基因,为研制人抗CD3人/鼠嵌合抗体完成了关键性的第一步.
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  恒区
     The heavy and light chain variable region (VH and VL) genes encoding the murine monoclonal antibody OH3 were combined with human γ3 and κ constant region genes to construct the murine human chimeric genes,respectively.
     鼠源单克隆抗体OH3重、轻链可变区(VH、VL)cDNA分别与人免疫球蛋白恒区γ3、κcDNA拼接成人-鼠嵌合抗体基因。
短句来源
     Methods Six primers for I gH variable region gene families VH1~VH6 and one primer of com mon join region (J) were used to perform reverse transcriptase polymerase chain reaction(RT-PCR). Five oligonucleotides of constant region gene (Cμ, Cδ,Cγ, Cα and Cε) were also used as reverse primers.
     方法 患者血清蛋白电泳发现 3个单克隆免疫球蛋白峰 ; RT PCR采用IgHV 6个家族 (VH1~VH6)正向引物 ,通用J区反向引物 ,IgH恒区Cμ、Cδ、Cγ、Cα和Cε 5种特异性反向引物。
短句来源
     Methods Clone the gene encoding variable region of immunoglobulin from murine hybridoma cells by RT-PCR and RACE methods. Construct human-mouse chimeric antibody by fusing the cloned gene with the gene encoding the constant region of human immunoglobulin.
     方法采用RT-PCR及RACE技术从鼠杂交瘤细胞克隆免疫球蛋白可变区基因,与人免疫球蛋白恒区基因拼接,构建人-鼠嵌合抗体基因,转染CHO细胞并测定其表达量。
短句来源
     In this paper, human immunoglobulin kappa chain constant region (human C_kgene) was amplified by PCR with a set of primers and cloned into secretion expressionvector pINIII-ompA2 in frame, then expressed in three kinds of E. coli strains.
     本文应用聚合酶链反应(PCR)技术体外大量扩增人免疫球蛋白轻链恒区基因(人C_k基因),并将该基因克隆至分泌型表达载体pINⅢ-ompA2,转入大肠杆菌中表达。
短句来源
     PCR-AMPLIFICATION AND SECRETION EXPRESSION OF HUMAN IMMUNOGLOBULIN KAPPA CHAIN CONSTANT REGION
     人免疫球蛋白轻链恒区基因的体外扩增及其在大肠杆菌中的分泌表达
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  “constant region”译为未确定词的双语例句
     According to the sequence of porcine IgG H chain gene (CH2)(CH3) sequence(SSU03780) in GenBank,a pair of primers containing KpnI and Hind III restriction sites respectively were designed. The constant region of IgG heavy chain of porcine CH2 and CH3 domains were cloned from Beijing Changbai crossbred swine mesentery lymph node by RT-PCR.
     根据GenBank中注册的猪IgG H链基因cDNA CH2 CH3序列(SSU03780),设计了含KpnⅠ和HindⅢ酶切位点的一对引物,采用反转录-聚合酶链式反应(RT-PCR)法从北京长白杂交猪肠系膜淋巴结细胞扩增猪IgG H链基因CH2 CH3序列.
短句来源
     The 2 8 kb Eco RⅠ/ Eco RⅠ fragment of Tx gene showed that it was highly homologous with Ig kappa constant region. Based on the restriction map of Tx gene,another Xho Ⅰ/ Eco RⅠ Tx 3 0 fragment was sequenced and analyzed by bioinformatics.
     在对其中 2 .8kb片段测序的基础上 ,对其中 Xho /Eco R 长度约 3.0 kb的片段 ( Tx3.0 )进一步进行了测序 ,并利用生物信息学技术分析认为 ,Tx3.0与人类免疫球蛋白 kappa( Igκ)轻链基因高度同源 ,并直接映射于 J区 .
短句来源
     Comparison of NPC Transforming Gene Tx to Ig Kappa Constant Region Gene and Their Expressions in Different Cell Lines
     Tx基因与IgK基因的同源性研究及其在不同细胞株的表达
短句来源
     We have compared the nucleotide sequence and amino acid sequence of coding product of human immunoglobulin light chain kappa constant region gene(IGKC) to those of human nasopharyngeal carcinoma(NPO) transforming gene(Tx gene)2.8kb EcoR I fragment.
     本文对以前报道的Tx基因2.8kb片段的核苷酸序列与人免疫球蛋白kappa链C区基因的核苷酸序列及其编码产物的氨基酸序列进行了同源性比较。
短句来源
     The result indicated that there were at least seven C_λ segments in the bovine immunoglobulin lambda light chain constant region.
     这一结果表明牛免疫球蛋白lambda轻链至少含有7个C_λ区,跨越了轻链大小约40kb的范围。
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  constant region
Immunoglobulin heavy chain constant region (IgCH) allotypes have also shown some associations with MG.
      
To reduce mouse components of 4C10, the constant region was replaced by a human constant domain to form the murine/human chimeric anti-id antibody TVE-1.
      
Omalizumab is a humanized mouse monoclonal antibody that binds specificially to the constant region of the immunoglobulin (Ig)E heavy chain.
      
Lagomorph-constant region IgG allotypes: Shared e determinants inOchotona sp
      
Linkage of murine (T,G)-A- -L-specific idiotypic determinants to the heavy chain constant region allotypic markers
      
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With the guanidinium isothiocyanate method,total RNA was isolated from hybridoma cells that secrete monoclonal antibody against Brucella melitenses.Poly (A)+RNA was obtained by oligo (dT)-cellulose affinity chromatography.Reverse transcriptase reaction was performed with a primer 3'A-T-A-G-G-T-G-A-C-C 5' that is complement to the codons of No.122-125 amino acid residues in 5' terminus of constant region.The size of synthesized ds-cDNA is about SOObp,that is consistent with the length of variable region...

With the guanidinium isothiocyanate method,total RNA was isolated from hybridoma cells that secrete monoclonal antibody against Brucella melitenses.Poly (A)+RNA was obtained by oligo (dT)-cellulose affinity chromatography.Reverse transcriptase reaction was performed with a primer 3'A-T-A-G-G-T-G-A-C-C 5' that is complement to the codons of No.122-125 amino acid residues in 5' terminus of constant region.The size of synthesized ds-cDNA is about SOObp,that is consistent with the length of variable region genes of heavy chain.The ds-cDNA was inserted into plasmid pUC19 with dC:dG tailing method,and the inserted plasmid was used to transform E.colt HB101.It has been proved that the insert was a variable region gene of heavy chain by clone hybridization in situ,size of insert and Southern blot.

用异硫氰酸胍法从分泌单克隆抗体的杂交瘤细胞中提取总RNA,经oligo(dT)-纤维素柱亲和层析获得poly(A)~+ RNA后,用恒定区5′端第122—125号氨基酸密码的互补序列3′A-T-A-G-G-T-G-A-C-C 5′做为引物,进行逆转录酶反应,合成双链cDNA,大小为300bp左右,与重链可变区基因的长度相符。用dC:dG接尾的方法,将ds-cDNA插入pUC19质粒,转化E.coli HB101。分离出重组体之后,经菌落原位杂交,酶切重组质粒DNA及Southern印迹,证明插入片段是重链可变区基因。

A murine/human chimeric antibody with specificity for hepatitis B surface antigen has been produced by genetic engineering. The light and heavy chain variable region exons encoding the murine monoclonal antibody 2H1 were isolated and inserted into mammalian expression vectors containing the human kappa and gamma 1 constant region exons. The chimeric genes were transfected into murine SP2/0 hybridoma cells by electroporation, and transfectomas secreting chimeric antibody were isolated. Secretion levels...

A murine/human chimeric antibody with specificity for hepatitis B surface antigen has been produced by genetic engineering. The light and heavy chain variable region exons encoding the murine monoclonal antibody 2H1 were isolated and inserted into mammalian expression vectors containing the human kappa and gamma 1 constant region exons. The chimeric genes were transfected into murine SP2/0 hybridoma cells by electroporation, and transfectomas secreting chimeric antibody were isolated. Secretion levels ranged from 1~ 7pg/(cell·24hr). The chimeric antibody bound specifically to hepatitis B surface antigen and competed effectively with the parental murine monoclonal antibody for binding to these sites. Chimeric 2H1 is the first clinically relevant, genetically engineered anti-viral antibody and may represent an improved agent for the prevention of hepatitis B virus transmission.

用抗体工程法构建抗乙肝表面抗原(HBsAg)鼠/人嵌合抗体的嵌合基因,并在哺乳动物细胞中成功地表达。分离鼠McAb 2H1(抗HBsAg)轻、重链可变区基因,并连接到含有人轻链(Kappa)及重链(Gamma 1)恒定区基因的哺乳动物细胞表达载体中;用电穿孔法将嵌合基因导入小鼠SP2/0细胞,得到高效表达。2H1嵌合抗体能特异性地与HBsAg结合,并能有效地与亲代McAb竞争;与血源性多克隆乙肝免疫球蛋白(HBIG)比较,其具有特异性好、节约血源、价廉、质纯、无其他病毒,如HIV等污染制品的可能等优点,可用于阻断乙肝母婴传播及在意外感染时被动免疫。

On the basis of considering biosystem consisting some characteristics of fuzzy control system, this paper tried to make the research on 29 kinds of animal cytochrome c molecular evolution with Fuzzy Cluster Analysis (FCA) method and Construct their phylogenetic tree.In order to enhance the precision of constructing biological macromolecular phylogenetic trees by FCA method, according to the principle of three nucleotides determining one amino acid, we did the operation of the datum standardization of maximal...

On the basis of considering biosystem consisting some characteristics of fuzzy control system, this paper tried to make the research on 29 kinds of animal cytochrome c molecular evolution with Fuzzy Cluster Analysis (FCA) method and Construct their phylogenetic tree.In order to enhance the precision of constructing biological macromolecular phylogenetic trees by FCA method, according to the principle of three nucleotides determining one amino acid, we did the operation of the datum standardization of maximal similarity values (Am) between 20 kinds of common amino acids during the evolution. After Am values being determined, the maximal similarity values (S_(AB)) between two amino acid sequences can be obtained. Then we can construct the phylogenetic tree by FCA method, through the S_(AB) values of the sequences. To get the ideal phylogenetic tree, we also used Arbitrary Division method to improved repeatedly the obtained phylogenetic tree and finally we got the phylogenetic tree after the improvement. Comparing our result with the phylogenetic trees obtained by other methods, we can see that, they are agreement, and our getting the phylogenetic tree of cytochrome c is also very similar to that obtained by the fossil records.In view of biosystem being a fuzzy control system and the biological macromolecular evolution having some fuzzinesses at different degrees, on the one hand, using fuzzy mathematical principle and method to explore and discuss the evolutionary relationship among biological macromolecules is significant, and this method not only considered the direct relationship among these molecular sequences, but also involved the indirect relationship among them. In addition, our result also indicated that the precision of the phylogenetic tree is not lower than those obtained by other theoretical computing methods. However, on the other hand, because of biosystem non-linearity, when some linear mathematical models and methods are used in the research on molecular evolution, it will be unavoidable that we would obtained some results which are different from or are not all the same as the real conditions, Therefore, when different persons researched the evolutionary relationship among the same group of biological macromolecules with different methods or indexes, they are often to get different results, and these results are not also all the same as those from the fossil records. One of the reasons causing the condition is the nonlinear relationship to be treated as the linear relationship when the molecular evolutionary problems are studied, another reason of leading to the error is resulted from the limitations of the research based on the primary structural comparison and analysis. The results of comparing and analyzing sequences have shown that the difference among primary structures of some homologous macromolecules, such as, 5S rRNA, which is existed in the closer species in evolution, is greater than that of those far- ther species in evolution. In addition to it, theprimary structures of some species are not lone,sometimes the nuclectide or amino acid residue at a site may be varied. For example, in the cytochrome c sequence of Rattlesnake, which total number of the residues is 104, but there may be two types of amino acids occurring at 13 sites of this sequence. The same condition could be also seen in some other cytochrome c sequences. On the contrary, the sequences in some different species are all same with each other, such as, seal and elephant; pig, bovine and sheep etc.The studies indicated, in cytochrome c molecules of more than 60 species, that over 1/3 amino acid residues in the sequences are same, which is named the constant region, and other residue sites are in the variable region. However the variable region of cytochrome c belongs in the tertiary structure, so it is very difficult to describe precisely their differences at the level of the primary structure. It is well known that biological macromolecular primary structures are the fundament of their spatial structures and their specific functio

本文在强调生物系统自身特点的基础上,针对分子进化本身所具有的模糊性,运用模糊聚类分析的原理和方法,就29种动物细胞色素c一级结构间的进化关系进行了研究,作出了相应的分子系统树并运用硬划分法对该树进行了修改。同时,与其它一些研究的结果进行了比较,讨论了在生物大分子一级结构间进行比较、分析的局限性和分子进化研究中的一些有待解决的问题。

 
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