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former plate
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  “former plate”译为未确定词的双语例句
     Methodological characteristics of former plate attachment to isolate epidermal stem cells
     应用原瓶黏附分选表皮干细胞的方法学特点
短句来源
     Two rhesus monkeys of 3 months old were adopted to isolate and culture their epidermal cells. The 2nd-4th passage cells after digestion were relayed in former plate for 20 minutes to harvest rapid attaching cells.
     健康3个月龄恒河猴2只,分离与培养猴表皮细胞,获取第2~4代的细胞,消化后,将细胞重新放回原瓶中,培养箱中静置20min,获得的贴壁细胞即为快吸附的表皮细胞。
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  相似匹配句对
     The former infectsG.
     前者只侵染海岛棉;
短句来源
     Error Analysis of Contouring Turn Plate Cam Former Face
     仿形车削盘形凸轮型面的误差分析
短句来源
     plate structure ?
     第二部分:绘画中“板块结构”原理的探究
短句来源
     The former has its limitation.
     “企业价值最大化”有其局限性 ;
短句来源
     Gold plate
     “镏”金岁月
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  former plate
Pole calculations using both right- and left-stepping fracture zones show an offset of 1-2 km between the deepest, most linear part of a fracture zone trough and the former plate boundary location.
      
The architecture of the European Variscides has been subdivided by Kossmat (1927) into paleogeographically coherent units which are presently interpreted as former plate fragments.
      
Alpine-type peridotites appear at present and former plate boundaries of all types.
      
A strong linear feature striking NW-SE at 660 km depth appears to be a former plate boundary.
      


This paper introduced a method of machiningcomplex curved surface through former plate and fixtures.And a new train of thought to machine special curved sur-face was opened up.

本文介绍用靠模夹具修正砂轮磨削复杂曲面的方法,提出了解决特殊型面加工的新思路。

CAE analysis of the process of injection molding of the refracting plate for JETTA Car was made,the cause for the sink mark and trapped gas of the molded plate was analyzed and the way to avoid the defects was proposed.In order to pack validly and compensate the shrink,the packing curve was determined according to the time for the last filled part to reach non-flow temperature and the time for the gate to reach frozen temperature from the pre-analysis results.Observing the part in the middle phase,we found that...

CAE analysis of the process of injection molding of the refracting plate for JETTA Car was made,the cause for the sink mark and trapped gas of the molded plate was analyzed and the way to avoid the defects was proposed.In order to pack validly and compensate the shrink,the packing curve was determined according to the time for the last filled part to reach non-flow temperature and the time for the gate to reach frozen temperature from the pre-analysis results.Observing the part in the middle phase,we found that the trapped gas was the results of the gathering of small air bubbles.The results from CAE analysis and validating test showed that the defects of the refracting plate were due to the temperature difference and volume shrinkage difference between the surface and the inside.Because the gate thickness of the former plate was thin,the gate froze quickly during the molding,and the material could not be compensated in the packing phase.By enlarging the dimension of the gate,the quick freeze of the gate could be avoided,making the temperature and volume shrinkage between the surface and inside uniform,resulted in elimination of the defects in the plate by packing.

对捷达轿车车灯上的折光板生产过程进行了CAE分析。分析了产生缩痕、气泡等缺陷的原因,提出了抑制缺陷的方法。为最大限度地补料,保压曲线的确定是经过一次预分析,通过确定最后充填部分树脂冷却到不流动的温度所需要的时间及浇口处树脂冷却到凝固温度所需要的时间确定的。观察实际生产过程发现,产品内部的气泡是由于生产过程中小气泡集中的结果。分析及实际验证结果表明折光板的缺陷是由于注射成型结束时产品中心部和表面温差、体积收缩差大造成。原有产品的浇口厚度薄,过早凝固,无法通过保压及时补料,采用扩大尺寸的直浇口,可以避免浇口过早凝固,使产品中心部和表面的温差及体积收缩均匀分布,进而通过保压消除了由于收缩产生的缺陷。

AIM: To look for an easy and feasible approach to isolate epidermal stem cells (ESCs), which may provide a practical source of further researches on tissue engineering. METHODS: From July to December 2005, the research was completed in National Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center of Sun Yat-Sen University. Two rhesus monkeys of 3 months old were adopted to isolate and culture their epidermal cells. The 2nd-4th passage cells after digestion were relayed in former plate for 20 minutes...

AIM: To look for an easy and feasible approach to isolate epidermal stem cells (ESCs), which may provide a practical source of further researches on tissue engineering. METHODS: From July to December 2005, the research was completed in National Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center of Sun Yat-Sen University. Two rhesus monkeys of 3 months old were adopted to isolate and culture their epidermal cells. The 2nd-4th passage cells after digestion were relayed in former plate for 20 minutes to harvest rapid attaching cells.①ESCs before isolation and rapid attaching cells were observed on a microscope.②The isolated rapid attaching cells with collagen type IV adhesion were measured by flow cytometry for integrin α6 and CD71, and the cells without attachment were taken as controls.③Rapid attaching cells and non-attaching cells were respectively put upon three slides. Immunohistochemistry staining was conducted to detect integrin β1, K10 and K15.④Reverse transcription polymerase chain reaction (RT-PCR) was used to identify the expression of specific markers (β1, K15 and K1) on the isolated rapid attaching cells and non-attaching cells. RESULTS: ①The rapid attaching cells exhibited small cell size with a high nuclear-to-cytoplasmic ratio.②The results of flow cytometry showed that putative ESCs (α6bri CD71dim=82.5±1.641) were predominant in rapid attaching cells, transient amplifying cells (α6bri CD71bri=4.9±2.125) were relatively scarce, and postmitotic differentiating cells and terminally differentiated cells (α6dim) were not existing.③The immunohistologic experiments showed that rapid attaching cells isolated by type IV collagen attachment method expressed K15 and integrin β1 respectively. However, non-attaching cells expressed K10 only.④RT-PCR showed that the rapid attaching cells were positive for K15 and integrin β1, while the cell after isolation were positive for K10 only. CONCLUSION: Former plate attachment method is an easy and feasible approach to isolate ESCs.

目的:探索一种简单易行的方法分离表皮干细胞,为组织工程学的进一步研究奠定基础。方法:实验于2005-07/12在中山大学眼科中心国家眼科学重点实验室完成。健康3个月龄恒河猴2只,分离与培养猴表皮细胞,获取第2~4代的细胞,消化后,将细胞重新放回原瓶中,培养箱中静置20min,获得的贴壁细胞即为快吸附的表皮细胞。①于光学显微镜下观察分选前细胞和快吸附细胞。②对20min快吸附的表皮细胞进行整合素α6和CD71流式细胞仪检测,以未经吸附分选的细胞作为对照。③20min快吸附的表皮细胞和分选后未黏附细胞分别制作3组细胞爬片,进行整合素β1、K15、和K10的免疫组织化学染色。④20min快吸附的表皮细胞和分选后未黏附细胞进行K15、整合素β1和K1(K10对应角蛋白)的mRNA反转录聚合酶链反应的检测。结果:①分选后的细胞体积较小,核浆比例较大。②流式细胞仪的结果显示快吸附细胞以干细胞为主(α6briCD71dim为82.5±1.641),也含有少量的短暂扩增细胞(α6briCD71bri为4.9±2.125),有丝分裂后细胞及终末细胞则检测不到(α6dim)。③免疫组化可见快吸附细胞呈K15和整合素β1阳性,K1...

目的:探索一种简单易行的方法分离表皮干细胞,为组织工程学的进一步研究奠定基础。方法:实验于2005-07/12在中山大学眼科中心国家眼科学重点实验室完成。健康3个月龄恒河猴2只,分离与培养猴表皮细胞,获取第2~4代的细胞,消化后,将细胞重新放回原瓶中,培养箱中静置20min,获得的贴壁细胞即为快吸附的表皮细胞。①于光学显微镜下观察分选前细胞和快吸附细胞。②对20min快吸附的表皮细胞进行整合素α6和CD71流式细胞仪检测,以未经吸附分选的细胞作为对照。③20min快吸附的表皮细胞和分选后未黏附细胞分别制作3组细胞爬片,进行整合素β1、K15、和K10的免疫组织化学染色。④20min快吸附的表皮细胞和分选后未黏附细胞进行K15、整合素β1和K1(K10对应角蛋白)的mRNA反转录聚合酶链反应的检测。结果:①分选后的细胞体积较小,核浆比例较大。②流式细胞仪的结果显示快吸附细胞以干细胞为主(α6briCD71dim为82.5±1.641),也含有少量的短暂扩增细胞(α6briCD71bri为4.9±2.125),有丝分裂后细胞及终末细胞则检测不到(α6dim)。③免疫组化可见快吸附细胞呈K15和整合素β1阳性,K10阴性;分选后未黏附细胞K10为阳性,而K15和整合素β1均为阴性。④反转录聚合酶链反应示吸附分选后的快吸附细胞高表达K15和β1整合素,K10没有表达;而分选后剩余的细胞只表达K10。结论:原瓶黏附分选方法是一种简单可行的分选表皮干细胞的方法。

 
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