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fumaric     
相关语句
  富马酸
     In shake flask culture, L-Malic acid yields was 10.49 g/100ml at 32℃ for 96h, and the conversion rate of fumaric acid was 90.80%.
     在摇瓶培养条件下,32℃ 96小时,产L-苹果酸达10.49g/100ml,对富马酸的转化率达90.80%。
短句来源
     Synthesis of fumaric acid by isomerization of maleic anhydride with KBr-H_2O_2 as catalyst
     KBr-H_2O_2催化顺酐异构化合成富马酸
短句来源
     After the anaerobic hydrolytic treatment, at 18h , the CODcr removal rate of fumaric acid-containing organic wastewater reached: H1: 72.3%, H2: 70.2%, H12: 88.2%.
     厌氧水解酸化后的富马酸废水再经好氧处理的COD去除率(处理18h):H1:72.3%,H2:70.2%,H12:88.2%。
短句来源
     The optimal conditions were obtained as follows:mole ratio of fumaric acid to linoleic acid (1.1-1.2):1,reaction temperature 180-190℃, reaction time 2-3 h,amount of iodine 0.15%-0.2% of the fatty acid of cottonseed oil.
     最佳合成条件为:n(富马酸):n(亚油酸)=(1.1~1.2):1,反应温度180~190℃,碘的用量(与棉籽油脂肪酸质量比)0.15%~0.2%,反应时间2~3h;
短句来源
     molar ratio of methanol to acid was 7∶1,mass ratio of catalyst to fumaric acid was 6∶100,reaction time was 6 h, the yield of dimethyl fumarate can reached 88.1%.
     酯化反应中n(甲醇)∶n(富马酸)=7∶1,m(PW12/C)∶m(富马酸)=6∶100,回流反应6h,富马酸二甲酯产率达88.1%。
短句来源
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  延胡索
     Under the optimal condition, the average conversion rate of fumaric acid was 84.21%, and L-malic acid yield was 11.13g/100mL
     延胡索酸平均转化率84.21%,平均产L-苹果酸11.13/100mL。
短句来源
     Optimal medium of Rhizopus arrhizus R25 for the production of fumaric acid consist of 12% glucose, 0.1%urea, 0.075%corn steep liquor, 0.03% KH2PO4, 0.02-0.04% MgSO47H2O, 50ppm ZnSO4 7H2O, 5.0%CaCO. and 1.5% methanol.
     无根根霉(Rhizopus arrhizus)R_(25)产延胡索酸适宜的条件为:葡萄糖12%,尿素0.1%,玉米浆0.075%,KH_2PO_4 0.03%,MgSO_4·7H_2O 0.02-0.04%,ZnSO_4·7H_2O 50ppm,CaCO_2 5.0%,甲醇1.5%。
短句来源
     The optimal condition is: concentration of fumaric acid 11%; aeration velocity(v/v/m) at 1:1(0-48h) and 1:1.25 (48-102h); temperature 30℃(0-48h) to 25℃ (48-102h), original pH 6.0-6.1, speed of agitator, 240 r/min, fermentation time 102 hours.
     确定了最优工艺条件,其中延胡索酸浓度11%,通风量1:1(培菌阶段)-1:1.25v/v/m(产酸阶段),温度30(培菌阶段)-25℃(产酸阶段),初始pH6.0-6.1,搅拌转速240r/min,发酵周期102h左右。
短句来源
     Fermentation Conditions of Rhizopus arrhizus R_(25) for Fumaric Acid Production
     无根根霉R_(25)产延胡索酸的发酵条件
短句来源
     The small calli finally were subcultured in white agar medium supplemented with mixture of pyruvic acid,citric acid and fumaric acid(5 mmol/l); 1mg/l 2,4-D and 4% glucose. They could be differentiated thoroughly into normal tissue.
     最后移入 White 培养基添加丙酮酸、延胡索酸与柠檬酸三者混合物(5mmol/l)及1mg/l 2,4-D与4%葡萄糖后,始呈现彻底的脱分化状态,愈伤组织才能正常生长。
短句来源
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  富马
     Synthesis of Fumaric Acid Modified Rosin
     富马松香的合成
     2.the DM of maleic rosin and fumaric rosin with a dosage of 15 percent is 37%;
     ②加成量为w=15%的马来松香和富马松香的改性度均为37%;
短句来源
     The test results show that:1.With the increase of the dosang of modifier,the acid value,saporification value and softering temperature of fortified rosin increase linearly; 2.It is preferable to control the dosage of maleic rosin and fumaric rosin over a range from 3% to 5%. 3.The leading compositions of fumaric rosin are rosin acid and fumaric acid,besides rosin scid and maleic acid,maleic anhydride and maleic ester are also the leading compositions of maleic rosin.
     研究结果表明:①强化松香的酸值,皂化值和软化点都随着加成量的增加而提高,基本上呈线性关系:②强化松香的马来酸或富马酸的加成量应控制在3%~5%范围内为宜:③富马酸的主要成分为松香酸和富马海松酸,马来松香的主要成分除了松香酸和马来海松酸以外,还有马来海松酸酐和马来海松酸酯;
短句来源
     4.Fumaric acid has a lower softening temperature and saponification value but a higher acid value,which was beneficial to improving the quality of modified rosin size and dispersed rosin size.
     ④在加成量相同的情况下:富马松香与马来松香相比具有较低的软化点和皂化值,却有较高的酸值,有利于改善强化松香胶和分散松香胶的质量。
短句来源
     By the measurment of soften temperature, the infrared spectrometric analysis and other experiments, it is proved that under the optimal conditions a fumaric acid modified rosin with high soften temperature, high acidity and light color is obtained.
     由软化温度的测试和红外分析等实验证明,在此条件下可得到软化温度和酸值较高及色泽较浅的富马松香。
  反丁烯二酸的
     Content Determination of Fumaric Acid in Sukebing Oral Liquid
     速克痛口服液中反丁烯二酸的含量测定
短句来源
     The content of maleic acid and fumaric acid in the root exudates were very low, and Mn deficiency treatment and low Mn treatment didn't increase the content of these two organic acids in the wheat root exudates.
     根分泌物中顺丁烯二酸和反丁烯二酸的含量极低,缺锰和低锰胁迫不能促进这两种有机酸分泌量的增加。
短句来源

 

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      fumaric
    New Derivatives of Fumaric Acid as Plant Growth Regulators
          
    Voltammetric Determination of Maleic and Fumaric Acids at an In Situ Renewed Graphite Electrode
          
    It was shown that a graphite electrode renewed by cutting a thin surface layer immediately before each measurement can be used for the voltammetric determination of maleic and fumaric acids.
          
    The relative standard deviation in the determination of maleic and fumaric acids at the in situ renewed graphite electrode was 3-6%, and the detection limits for both acids were 0.4 mg/L.
          
    Decomposition of KF · H2O2 stabilized with carboxylic acids (maleic, fumaric, succinic, adipic) in storage for more than three years at 20°C was studied.
          
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    The study of the paper chromatography of organic acids was undertaken to provide a simplemethod for the identification of the organic acids in plant tissues.With the technique of ascend-ing development,the R_f values of seven organic acids were determined as follows:With the exception of the R_f values of oxalic acid,these values agree generally with thosefound in the literature.Temperature variations have no apparent influence on the R_f values ofthe organic acids examined.Different from other organic acids,oxalic...

    The study of the paper chromatography of organic acids was undertaken to provide a simplemethod for the identification of the organic acids in plant tissues.With the technique of ascend-ing development,the R_f values of seven organic acids were determined as follows:With the exception of the R_f values of oxalic acid,these values agree generally with thosefound in the literature.Temperature variations have no apparent influence on the R_f values ofthe organic acids examined.Different from other organic acids,oxalic acid left long trails on the chromatograms andthis trailing was not eliminated by an increased concentration of the swamping acid HCOOH.Experiments have shown that only when the concentration of the oxalic acid solution applied issufficiently high(above 0.5 M),can a well defined spot be seen at the end of the trail and onlythis spot appears to have a constant R_f value.On the other hand,when the concentration ofthe test solution is comparatively low(below 0.5 M),no spot can be seen on the chroma-togram,except a diffuse trail,the length of which is found to vary with the concentration of theacid applied,being less at the lower concentrations.The R_f values of oxalic acid reported inthis paper are obtained on the condition that only concentrated solutions(above 0.5 M)of theacid are used and only the excursions of the well defined spots at the ends of the trails are basedfor calculations.A modified method for the chromatographic detection of organic acids from plant tissuesis here described.In order to overcome the difficulty in differentiating the acids of similarmobilities and to avoid the possible loss of acids from detection,it is proposed to develop twochromatograms for each sample with two different solvents and also to compare the resultsobtained with both diluted and concentrated solutions of the unknown acids.By this method the leaves of castor-oil plant(Ricinus communis,L.)were found to containtartaric acid,citric acid,malic acid,and fumaric acid and those of lotus(Nelumbo nucifera,Gaertn)were found to contain gluconic acid,tartaric acid,citric acid,malic acid,oxalic acid andsuccinic acid.

    (1)本文分别用戊醇-蚁酸-水和丁醇-蚁酸-水为溶剂测定了酒石酸等七种有机酸的 R_f 值。除草酸外,所有结果与文献记录基本符合。在本文实验的温度范围内(30±4°)温度变化对有机酸的 R_f 值并无显著影响。(2)由于草酸的严重拖尾现象,在一般的操作用量下,草酸试液浓度在0.5M 以下不能生成有固定 R_f 值的斑点。因此草酸 R_f 值必须以0.5M 以上的浓试液进行试验,并须以最高处的深浓斑点作为计算根据。(3)对植物组织中非挥发性有机酸的纸上层析,本文建议用两种 R_f 值相互验证,并用浓试液与稀试液作比较试验,所得结果比较明确可靠。(4)用本方法在蓖麻叶中检出了酒石酸,柠檬酸,苹果酸和延胡索酸四种非挥发性有机酸。在荷叶中检出了葡萄糖酸,酒石酸,柠檬酸,苹果酸,草酸和琥珀酸六种非挥发性有机酸。

    (2-C~(14))-acetate was fed to etiolated rice seedlings and the respiratory path- ways of the acetate in them were studied.The following results were obtained from feeding experiments: 1.5-day old etiolated rice seedlings were extracted with 80% alcohol.The extract was passed through the anion exchange resin Amberlite IRA-400 and the eluant was col- lected for chromatography on Sin Hua no.1 filter paper.The paper chromatogram showed that citric,isocitric,α-ketoglutaric,succinic,fumaric and malic acids were...

    (2-C~(14))-acetate was fed to etiolated rice seedlings and the respiratory path- ways of the acetate in them were studied.The following results were obtained from feeding experiments: 1.5-day old etiolated rice seedlings were extracted with 80% alcohol.The extract was passed through the anion exchange resin Amberlite IRA-400 and the eluant was col- lected for chromatography on Sin Hua no.1 filter paper.The paper chromatogram showed that citric,isocitric,α-ketoglutaric,succinic,fumaric and malic acids were present in the rice tissues.It demonstrates that the intermediates of the tricarboxylic acid cycle exist in the intact rice seedling. 2.The rice seedlings were fed with (2-C~(14))-acetate,incubated in various time in- tervals from 2 to 40 or 60 minutes and then extracted with 80% hot alcohol.The extracts were passed through cation exchange resin Zerolite 225 and anion exchange resin Amberlite IRA-400 to separate them into organic acids, amino acids and sugars. The estimation of the three kinds of compounds indicates that radio activity appeared first in the organic acids,followed by amino acids and rarely appeared in the sugars. 3.The above organic acids were fractionated with ion exchange chromatography of Zerolite FF.The fractions were collected and concentrated for radioactivity determina- tion.The results showed that citric,isocitric,succinic,fumaric and malic acids are labelled with C~(14).When malonate was added to the substrate solution,the incorporation of acetate into the organic acids was reduced to below 50%,while the radioactivity of the labelled succinate increased correspondingly.The radiochromatogram indicates the ac- cumulation of succinate in the rice seedlings fed with labelled acetate and malonate. 4.The radioactivities of the organic acids isolated from the rice seedlings showed that succinate appeared in the initial 2 minutes in quite measurable quantity,and ma- late and citrate increased in amount until 10 minutes later.Radiochromatogram con- firmed this result. 5.When arsenite and α,α′-dipyridyl were added to the media,the incorporation of acetate into organic acid was inhibited to 68% and 44% respectively,but the amount in- corporated into succinate was depressed 46% and 29% respectively.It means that some labelled succinate accumulates even as the inhibitors are operative. 6.The radiochromatogram of the amino acids extracted from the seedlings fed with (2-C~(14))-acetate shows that the radioactivities of aspartate and glutamate approach 17% and 20% of the total radioactivity of amino acids respectively.If malonate was added, the incorporation of C~(14) into amino acids was reduced to 50%.It means that the labelled acetate incorporates into those amino acids which are assiociated with the tricarboxylic acid cycle. 7.The following conclusion may be drawn from the above experimental results. Tricarboxylic acid cycle is present in the intact rice seedlings.From the facts that large amounts of succinate was labelled during the first moment of feedings and that some amount of succinate was still accumulated even after inhibition by arsenite and α,α′- dipyridyl,it is suggested that besides the tricarboxylic acid cycle as the main route for the utilization of acetate,it is probable that glyoxylate cycle and dicarboxylic acid cycle may be operative in these rice seedlings.

    以[2-C~(14)]-乙酸饲喂水稻黄化幼苗,研究了乙酸在体内的呼吸途径。从饲喂试验获得了以下结果:1.以80%酒精提取萌发5天的水稻幼苗,将提取液通过阴离子交换树脂 AmberliteIRA-400,收集洗脱液进行纸上层析。从层析谱上得到异柠檬酸、柠檬酸、α-酮戊二酸、琥珀酸、延胡索酸及苹果酸。结果表明在水稻幼苗中存在着三羧酸循环的中间成分。2.以[2-C~(14)]乙酸饲喂水稻幼苗,经过不同时间2至40或60分钟保温,立即以80%热酒精提取。提取液通过阳离子交换树脂 Zerolite 225及阴离子交换树脂 AmberliteIRA-400,以分离出有机酸、氨基酸及糖三类化合物。这三类化合物放射性强度的测量结果表明,放射性最初只在有机酸中出现,随后为氨基酸,而糖类几乎始终不出现放射性。3.从饲喂乙酸的水稻幼苗提取液中分离出的有机酸,用阴离子交换树脂 Zerolite FF进行柱层析,分部收集各种有机酸,浓缩并测量其放射性强度。结果表明柠檬酸、异柠檬酸、琥珀酸、延胡索酸及苹果酸均被 C~(14)标记上。当饲喂乙酸时,同时加入丙二酸,则乙酸渗入到有机酸的总量降至50%以下,而标记琥珀酸的放射性强度却相对增加,...

    以[2-C~(14)]-乙酸饲喂水稻黄化幼苗,研究了乙酸在体内的呼吸途径。从饲喂试验获得了以下结果:1.以80%酒精提取萌发5天的水稻幼苗,将提取液通过阴离子交换树脂 AmberliteIRA-400,收集洗脱液进行纸上层析。从层析谱上得到异柠檬酸、柠檬酸、α-酮戊二酸、琥珀酸、延胡索酸及苹果酸。结果表明在水稻幼苗中存在着三羧酸循环的中间成分。2.以[2-C~(14)]乙酸饲喂水稻幼苗,经过不同时间2至40或60分钟保温,立即以80%热酒精提取。提取液通过阳离子交换树脂 Zerolite 225及阴离子交换树脂 AmberliteIRA-400,以分离出有机酸、氨基酸及糖三类化合物。这三类化合物放射性强度的测量结果表明,放射性最初只在有机酸中出现,随后为氨基酸,而糖类几乎始终不出现放射性。3.从饲喂乙酸的水稻幼苗提取液中分离出的有机酸,用阴离子交换树脂 Zerolite FF进行柱层析,分部收集各种有机酸,浓缩并测量其放射性强度。结果表明柠檬酸、异柠檬酸、琥珀酸、延胡索酸及苹果酸均被 C~(14)标记上。当饲喂乙酸时,同时加入丙二酸,则乙酸渗入到有机酸的总量降至50%以下,而标记琥珀酸的放射性强度却相对增加,纸层析谱的放射自显影也证明有琥珀酸的积累。4.从不同时间饲喂乙酸所分离出的有机酸,其放射性强度测量结果表明,2分钟标记琥珀酸出现最多,至10分钟后,苹果酸和柠檬酸同时随之增加,纸层析谱的放射自显影也证实了这点。5.当饲喂乙酸时,同时加入亚砷酸钠或α,α′-联呲啶,则乙酸的利用受到不同程度的抑制,分别为68%及44%,而 C~(14)渗入琥珀酸的量只分别受到46%及29%的抑制,即施用抑制剂后仍有相当数量标记琥珀酸的积累。6.氨基酸的纸层析及放射性强度结果表明,天门冬氨酸及谷氨酸的放射性分别占氨基酸总放射性的17%和20%。饲喂乙酸时加入丙二酸,C~(14)渗入氨基酸下降50%以上,由此亦可见乙酸最早渗入到与三羧酸循环有直接联系的氨基酸中。7.从上述实验结果可以得出结论,在水稻整体幼苗中存在着三羧酸循环。同时根据短时间饲喂乙酸首先出现大量标记琥珀酸以及亚砷酸钠和α,α′联呲啶抑制后仍有较大量琥珀酸积累的事实,可以认为乙酸的利用除了主要通过三羧酸循环以外,还可能通过乙醛酸循环及二羧酸循环等途径。

    The existence of malic dehydrogenase and fumarase in the homogenate of Schistosoma japonicum was reported.The activities of malic dehydrogenase and fumarase in homogenates of the worms were determined. Under the experimental conditions (malate, 0.02M; cytochrome c, 1×10~(-5)M;phosphate buffer, 0.01M, pH 7.4), the values of the paired worms were as follows: Q_(o_2)(oxygen uptake μl/mg. N/hr.)=4.5; Q_p (oxaloacetate production, μmole/ mg. N/hr., determined as pyruvate)=0.26; Q_F (fumarate production, μmole/mg.N/...

    The existence of malic dehydrogenase and fumarase in the homogenate of Schistosoma japonicum was reported.The activities of malic dehydrogenase and fumarase in homogenates of the worms were determined. Under the experimental conditions (malate, 0.02M; cytochrome c, 1×10~(-5)M;phosphate buffer, 0.01M, pH 7.4), the values of the paired worms were as follows: Q_(o_2)(oxygen uptake μl/mg. N/hr.)=4.5; Q_p (oxaloacetate production, μmole/ mg. N/hr., determined as pyruvate)=0.26; Q_F (fumarate production, μmole/mg.N/ hr.)=2.5. On the basis of mg. nitrogen content, the activity of the female worms was found to be higher than that of the male worms.When malate was used as substrate, the combined addition of glutamate and NAD increased the rate of respiration by 110%. Aspartic acid, formed by coupling malic dehydrogenase to glutamic-oxaloacetic transaminase, was confirmed by paper chromatography.Upon the addition of NAD, the Q_o, was found to have increased 30% and lactic acid was produced in significant amount (Q_L=0.44μmole/mg. N/hr.). The result clearly showed that the linkage between malic dehydrogenase and lactic dehydrogenase did occur in S. japonicum.In addition, the reversible reaction of malic dehydrogenase has been studied. With the same coupling reaction, NADH, formed enzymatically by lactic dehydrogenase, reduced the oxaloacetate. Both malic and fumaric acids were identified by means of paper chromatography.Sb-58 showed an inhibition of 65% and 30% on malic dehydrogenase of S. japonicum in a final concentration of 10~(-3) and 10~(-4) M respectively. Thyroxine and mesotartaric acid inhibited this enzyme as well. Tartar emetic failed to show any inhibition on malic dehydrogenase even at 1×10~(-3)M.

    本文报告了日本血吸虫存在苹果酸脱氢酶与延胡索酸酶。在采用的实验条件下(苹果酸钠,0.02M;细胞色素c,1×10~(-5)M;磷酸盐缓冲液,0.01M,pH 7.4)测定了血吸虫匀浆的酶活力。合抱成虫的酶活力为:氧耗量Q_o(?)=4.5微升氧/毫克氮量/小时;草酰乙酸产生量(以丙酮酸测定值表示)Q_p=O.26微克分子丙酮酸/毫克氮量/小时;延胡索酸产生量Q_F=2.5微克分子延胡索酸/毫克氮量/小时。当雌雄虫分别测定时,在等氮量基础上,雌虫酶活力较雄虫者为高。当苹果酸钠用作底物时,合抱成虫的氧耗量可因加入谷氨酸钠及NAD而增高110%。于此同时,用纸层析法证明血吸虫之苹果酸脱氢酶可与谷-草转氨酶相互联系而产生天门冬氨酸。加入NAD可使合抱成虫的氧耗量增高30%,同时发现有相当量乳酸产生(Q_L=0.44微克分子乳酸/毫克氮量/小时)。以上结果表明在血吸虫匀浆存在苹果酸脱氢酶与乳酸脱氢酶的交互作用。此外,研究了虫苹果酸脱氢酶的可逆作用。借助于虫自身的乳酸脱氢酶使加入的NAD还原为NADH,然后此NADH又可将底物草酰乙酸还原。在以上情况下,用纸层析法鉴定其产物为苹果酸及延胡索酸。二巯基丁二酸锑钠(Sb-58...

    本文报告了日本血吸虫存在苹果酸脱氢酶与延胡索酸酶。在采用的实验条件下(苹果酸钠,0.02M;细胞色素c,1×10~(-5)M;磷酸盐缓冲液,0.01M,pH 7.4)测定了血吸虫匀浆的酶活力。合抱成虫的酶活力为:氧耗量Q_o(?)=4.5微升氧/毫克氮量/小时;草酰乙酸产生量(以丙酮酸测定值表示)Q_p=O.26微克分子丙酮酸/毫克氮量/小时;延胡索酸产生量Q_F=2.5微克分子延胡索酸/毫克氮量/小时。当雌雄虫分别测定时,在等氮量基础上,雌虫酶活力较雄虫者为高。当苹果酸钠用作底物时,合抱成虫的氧耗量可因加入谷氨酸钠及NAD而增高110%。于此同时,用纸层析法证明血吸虫之苹果酸脱氢酶可与谷-草转氨酶相互联系而产生天门冬氨酸。加入NAD可使合抱成虫的氧耗量增高30%,同时发现有相当量乳酸产生(Q_L=0.44微克分子乳酸/毫克氮量/小时)。以上结果表明在血吸虫匀浆存在苹果酸脱氢酶与乳酸脱氢酶的交互作用。此外,研究了虫苹果酸脱氢酶的可逆作用。借助于虫自身的乳酸脱氢酶使加入的NAD还原为NADH,然后此NADH又可将底物草酰乙酸还原。在以上情况下,用纸层析法鉴定其产物为苹果酸及延胡索酸。二巯基丁二酸锑钠(Sb-58)在最后浓度为10~(-3)M及10~(-4)M时,分别抑制血吸虫苹果酸脱氢酶活力约65%及30%。甲状腺素及中酒石酸对该酶均呈抑制作用。酒石酸锑钾在10~(-3)M时对酶活力无显著影响。

     
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