Object: To observe the changes of Bcl-2 and Bax expression, learning and memory abilities, TUNEL' S apoptosis cells in Alzheimer' s disease rats injected amyloid-beta protein at hippocampus and to investigate the protection and the mechanism of PMT on apoptosis of hippocampal neurons.
The expression of Aβ_(1-40) and ERα in the cortex, hippocampus CA_1, amygdale and Meynert nucleus were observed. Statistical analysis was performed with the analysis of variance (ANOVA) and screening method.
The test of between-subjects effects shows that ovariectomized and fornix-hippocampal fimbria transected group exists reciprocating effect on the expression of Aβ_(1-40) in the cortex , hippocampus CA_1, amygdale and Meynert nucleus (p<0.01) .
Results: Comparing to CCMD-3: the diagnostics results show that sensitivity is 76.5％, specificity is 83.9％, accuracy is 80.7％, false positive rate is 23.5％, false negative rate is 16.1％, positive predictive value is 78.7％, negative predictive rate is 82.2％.
Comparing to DSM-IV: the diagnostics results show that sensitivity is 78％, specificity is 84.2％, accuracy is 81.5％, false positive rate is 22.％, false negative rate is 15.8％, positive predictive value is 79.8％, negative predictive rate is 82.8％.
MAIN OUTCOME MEASURES:Positive expression percentage of Fas,Apo2.7 and Bcl- 2 on platelet membrane and the integral of mini- mental state examination(MMSE),Hasegawa's dementia scale(HDS) and activities of daily living(ADL) of two groups after therapy.
blue granules(the secondary antibody was labeled by FITC fluorescence) and red granules(the secondary antibody was labeled by Cy3 fluorescence) were found in the neuronal cells at the 3rd day after induced with betaamyloid 25-35 in the experimental group,which indicated the positive expression of cyclin A and cyclin B1.②The cyclin A and cyclin B1 expressed negatively in control group.
The positive rate of FHIT gene expression in normal colorectal tissue, adenoma and adenocarcinoma were 93.75%, 68.75% and 46.25%, respectively.
The positive rate of IgM, IgG and recovery phase neutralization antibodies of the cases were 3.7%, 44.4% and 59.5% respectively while those of the controls were 0%, 8.3% and 33.3% respectively.
The result detected by FCM indicated that after incubation for 0.5 h at 37°C, the positive rate in group 1 was 63%, while the other 2 groups were negative.
Results from Annexin-V-FLUOS staining on anti-Fas antibody-treated cells revealed that the staining-positive rate in the rAAV-antisense 6A8 cDNA-transduced SKW6 cells was decreased in comparison to that in the wild-type and the mock-transduced cells.
The testing results show that our method outperforms other methods mentioned in this paper on six aspects: detection accuracy, number of support vectors, false positive rate, false negative rate, training time and testing time.
The positive expression and the average absorbency of Fos and Jun in the operation group were significantly higher than that in the false operation group (P >amp;lt; 0.05).
VEGF positive expression ratio was 84.8% in iNOS positive expression cases, and the ratio was 35.3% in negative cases.
PI (Proliferating Index) and SPF (S-phase fraction) in the combined positive expression of iNOS and VEGF group was significantly higher than that in the combined negative group.
In the cells with only integrin-positive expression, YC25-PEI-CP9 mediated a higher transgene expression than PEI but lower than CP9-PEI.
In vivo gene transfer in tumor-bearing nude mice also demonstrated that the dual-targeting vectors showed a significantly enhanced transfection efficiency in tumors with positive expression of FGF receptors and integrins.