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labeled fibrinogen
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     HMSCs were labeled with BrdU.
     用5μmol/L的5溴2脱氧尿苷(BrdU)对培养的hMSCs进行连续培养标记。
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     CHOLESTEROL LABELED WITH RADIOIODINE
     以放射性碘标记的胆固醇
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     Fibrinogen (Fg) were measured.
     1(PAI!1)、纤维蛋白原(Fg)。
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     the hydrolization of fibrinogen or plasminogen;
     二是水解血纤维蛋白或纤溶酶原;
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     Methods PTE was induced in Male Sprague Dawley(SD) rats through injecting heated()~(125)iodine-labeled fibrinogen autologous thromboembolic into external jugular veins.
     方法经颈外静脉注入加热125I-标记纤维蛋白原自体血栓,复制大鼠PTE模型,随机分组如下:1)正常对照组;
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  labeled fibrinogen
Preoperative hemostatic data were obtained on 42 brain tumor patients and correlated with the subsequent occurrence of venous thrombosis detected with 1211-labeled fibrinogen leg scans.
      
Forty-six patients who underwent surgery for brain tumors were studied prospectively with 125I labeled Fibrinogen leg scans to detect postoperative venous thrombosis.
      
Whole-body radioscanning of the rat after intraperitoneal administration of 131I-labeled fibrinogen revealed the accumulation of radiolabeled material in the inflammed rat paw.
      
Binding assays were performed on PBMC using125I-labeled fibrinogen complexed with rabbit IgG (or as a control F(ab')2) anti-human fibrinogen.
      
Effect of bacterial polysaccharide from Pasteurella pestis on distribution of I131-labeled fibrinogen in mice with Crocker's sar
      
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The present study is designed to investigate the mechanism of the intraabdominal adhesion formation af-ter intraperitoneal chemotherapy with carboplatin and to get an effective prevention method. 125I-labeled fibrinogen(125I-F) was used as a tracer, the inflow of fibrinogen into peritoneal cavities, and influx and accumulation of fibrinogen/fib-rin in the peritoneal lining tissues were measured using radioimmunoassay after intraperitoneal chemotherapy with the carboplatin liquid or the carboplatin...

The present study is designed to investigate the mechanism of the intraabdominal adhesion formation af-ter intraperitoneal chemotherapy with carboplatin and to get an effective prevention method. 125I-labeled fibrinogen(125I-F) was used as a tracer, the inflow of fibrinogen into peritoneal cavities, and influx and accumulation of fibrinogen/fib-rin in the peritoneal lining tissues were measured using radioimmunoassay after intraperitoneal chemotherapy with the carboplatin liquid or the carboplatin mixture liquid. The percentage of circulating125I-F extravasated into the peritonealcavity was increased significantly in rats treated with carboplatin liqiud as compared with the control group. Accumula-tion of the125I-F in peritoneal lining tissues was also increased above control levels. However, in rats treated with carbo-platin mixture liqiud, the percentage of circulating125I-F that extravasated into the Peritoneal cavity and the accumulationof the FN in peritoneal1 lining tissues were decreased significantly. It is suggested that intraperitoneal chemotherapy withcarboplatin mixture liquid may inhibite the over increased fibrinogen influx and fibrin accumulation in peritoneal cavity,and therefore inhibite the intraabdominal adhesion formation. The liquid is a more suitable nuid for the intraperitonealchemotherapy of malignant tumour.

为探讨腹腔内化疗后腹腔粘连形成机理及其针对性预防措施。采用~(125)I-纤维蛋白原作为示踪标记物,放射免疫分析法比较了卡铂液或合有透明质酸钠、人体白蛋白的卡铂复合液腹腔内化疗后,腹腔内纤维蛋白原渗出量和腹膜表面纤维蛋白沉积量变化。结果:卡铂液腹腔内灌注可明显地增加腹腔内纤维蛋白原的渗出和腹腔表面纤维蛋白的沉积,卡铂液中卡铂浓度与腹腔内纤维蛋白原渗出和腹膜表面纤维蛋白的沉积量呈显著正相关。而卡铂复合液组腹腔内纤维蛋白原渗出量和纤维蛋白沉积量明显低于卡铂液组,与对照组接近。本文结果表明:卡铂复合液对腹腔内化疗中腹腔内纤维蛋白原渗出和纤维蛋白沉积量有明显的抑制作用,对抑制由于反复腹腔内化疗而引起的腹腔粘连有重要作用。

Objective To observe the dynamic changes of urokinase-type plasminogen activator(uPA) and its receptor(uPAR) in rats after pulmonary thromboembolism(PTE) and their relationship to spontaneous thrombolysis rate.Methods PTE was induced in Male Sprague Dawley(SD) rats through injecting heated()~(125)iodine-labeled fibrinogen autologous thromboembolic into external jugular veins.Thirty-five rats were randomly assigned into following groups(each 7): 1) Healthy control group;2) PTE group including PTE 4 h group,PTE...

Objective To observe the dynamic changes of urokinase-type plasminogen activator(uPA) and its receptor(uPAR) in rats after pulmonary thromboembolism(PTE) and their relationship to spontaneous thrombolysis rate.Methods PTE was induced in Male Sprague Dawley(SD) rats through injecting heated()~(125)iodine-labeled fibrinogen autologous thromboembolic into external jugular veins.Thirty-five rats were randomly assigned into following groups(each 7): 1) Healthy control group;2) PTE group including PTE 4 h group,PTE 24 h subgroup,PTE 3 d subgroup,PTE 5 d subgroup.The rats were separately sacrificed at 4 h,24 h,3 d,5 d after the PTE model was successfully made.Plasma samples were extrated and reserved for analysis of urokinase-type plasminogen activator(uPA) and its receptor(uPAR),so were hearts,lungs and whole blood for spontaneous thrombolysis rate.Results 1) Plasma concentrations of uPA and uPAR elevated after PTE(24 h P<0.05;3 d P<0.01;PTE 5 d uPA P<0.05,uPAR P<0.01)and reached a peak valuein PTE 3 d group,then began to decrease in PTE 5 d subgroup.Plasma concentration of uPA was positively correlated with spontaneous thrombolysis rate after PTE in 4 h,24 h,3 d subgroups(4 h r=0.758,P<0.05;24 h r=0.764,P<0.05;3 d r=0.778,P<0.05),so was plasma uPAR(4 h r=0.701,P<0.05;24 h r=0.764,P<0.05;3 d r=0.777,(P<)(0.05)).Histopathological results of the lung: Secondary thrombosis and infiltration of leukocyte in thrombus and vascular wall was observed in PTE 4 h subgroup.The injected clot was obviously dissolved in PTE 3 d and 5 d subgroup replaced by secondary thrombus.Conclusion 1) Plasma levels of endogenous uPA and uPAR are increased at different time points after PTE which promote endogenous fibrinolysis.

目的观察大鼠肺血栓栓塞症(PTE)后不同时间血浆尿激酶型纤溶酶原激活物(uPA)和尿激酶型纤溶酶原激活物受体(uPAR)的动态变化,并探讨两者与血栓自溶率的相关性。方法经颈外静脉注入加热125I-标记纤维蛋白原自体血栓,复制大鼠PTE模型,随机分组如下:1)正常对照组;2)PTE组:又分为PTE 4 h组(PTE 4 h)、PTE 24 h组(PTE 24 h)、PTE 3 d组(PTE 3d)、PTE 5 d组(PTE 5 d),即分别在造模成功后观察4 h2、4 h3、d、5 d后处死小鼠,留取血浆标本测定uPA、uPAR的水平;留取左肺观察肺组织病理改变;留取心脏、肺脏、全血以计算血栓自溶率。结果血浆uPA、uPAR水平在PTE后4 h略有上升(P>0.05),PTE后24 h明显增加(P<0.05),3 d时最高(P<0.01),5 d时开始下降,但仍明显高于正常对照组(uPAP<0.05,uPARP<0.01);PTE后4 h组、24 h组、3 d组血浆uPA质量浓度与血栓自溶率正相关(4 hr=0.758,P<0.05;24 hr=0.764,P<0.05;3 dr=0.778,P<0.05),血...

目的观察大鼠肺血栓栓塞症(PTE)后不同时间血浆尿激酶型纤溶酶原激活物(uPA)和尿激酶型纤溶酶原激活物受体(uPAR)的动态变化,并探讨两者与血栓自溶率的相关性。方法经颈外静脉注入加热125I-标记纤维蛋白原自体血栓,复制大鼠PTE模型,随机分组如下:1)正常对照组;2)PTE组:又分为PTE 4 h组(PTE 4 h)、PTE 24 h组(PTE 24 h)、PTE 3 d组(PTE 3d)、PTE 5 d组(PTE 5 d),即分别在造模成功后观察4 h2、4 h3、d、5 d后处死小鼠,留取血浆标本测定uPA、uPAR的水平;留取左肺观察肺组织病理改变;留取心脏、肺脏、全血以计算血栓自溶率。结果血浆uPA、uPAR水平在PTE后4 h略有上升(P>0.05),PTE后24 h明显增加(P<0.05),3 d时最高(P<0.01),5 d时开始下降,但仍明显高于正常对照组(uPAP<0.05,uPARP<0.01);PTE后4 h组、24 h组、3 d组血浆uPA质量浓度与血栓自溶率正相关(4 hr=0.758,P<0.05;24 hr=0.764,P<0.05;3 dr=0.778,P<0.05),血浆uPAR质量浓度与血栓自溶率正相关(4 hr=0.701,P<0.05;24 hr=0.764,P<0.05;3 dr=0.777,P<0.05)。肺组织病理改变:PTE后4 h有少量继发血栓形成,血栓及血管壁内可见白细胞浸润;PTE后3 d、5 d见注入的血凝块明显溶解,代之以新形成的血栓。结论PTE后血浆uPA、uPAR水平增加,促进内源性纤维蛋白溶解。

 
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