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microbial material
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     Material
     一、实验材料
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     Material
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     Develop the microbial limit tests on insoluble antibiotic material
     非水溶性抗生素原料的微生物限度检查方法及验证
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     Microbial forensics
     微生物物证检验
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     Microbial Biomineralization
     微生物成矿(英文)
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  microbial material
A thermotolerant species of Phormidium produced extracellular anti-microbial material during batch culture.
      
When the pure vitamin was used, the results obtained using all three methods were similar, but when samples from microbial material were used, the results were different.
      
Comparison of methods for determination of vitamin B12 in microbial material
      
Some of these invalid objects are just the neighboring non-microbial material that absorbs some of the stain or autofluoresce in the same color range.
      
Fungal and other microbial material is present on nearly all indoor surfaces.
      


As one of the special bioherms, mud mounds (micrite mounds) developed during the Paleozoic. According to recent researches, many experts in the world have believed that many mud mounds be built by different microbes in the Paleozoic, the lime mud sediments and the mud mounds built by cynobacteria have also been found in modern lakes. But the body fossils of microbes such as bacteria have not been seen in the Paleozoic mud mounds, and the function of the microbial biosedimentation & buildup, and how...

As one of the special bioherms, mud mounds (micrite mounds) developed during the Paleozoic. According to recent researches, many experts in the world have believed that many mud mounds be built by different microbes in the Paleozoic, the lime mud sediments and the mud mounds built by cynobacteria have also been found in modern lakes. But the body fossils of microbes such as bacteria have not been seen in the Paleozoic mud mounds, and the function of the microbial biosedimentation & buildup, and how the bacteria made carbonate deposition are not clear. During Early Paleozoic, there were many mud mounds developed in the north Sichuan. According to the SEM studies, the authors found three kinds of microbial body fossils from the Silurian mud mounds and the microbial biosedimentation:1.Smooth ball-like body fossil with egg shape,5μm × 3μm in size; 2. Rough ellipsoid fossil, 60μm×50μm in size; 3. Foamed network-like fossil formed by foamed network biofilm material covered the micrite. The biomarks analyses also proved that the microbial materials are in mud mounds. The microbial body fossils of mud mounds shower different functiosn of biosedimentation & buildupmentation. The studies confirmed the microbes were very important in the mud mound formation in Paleozoic.

作为生物礁的特殊类型 ,灰泥丘在古生代十分发育 ,许多学者都认为它们是通过微生物造岩作用而形成的 ,而且 ,也在现代湖泊中发现了由兰菌藻的生命活动所形成的灰泥沉积物及灰泥丘。但是国际上一直没有在古生代灰泥丘中发现过细菌等微生物的实体化石 ,微生物促使碳酸钙沉淀、聚集的能力和机理也不明确。四川盆地北缘寒武纪和志留纪灰泥丘发育 ,笔者通过扫描电镜及能谱分析 ,在国际上首次发现了 (川西北志留纪 )灰泥丘中菌藻类微生物的实体化石及其特征的造岩成丘现象 ,发现的菌藻类微生物实体化石有三种类型 :1.表面光滑的卵形球体型 ;2 .表面粗糙的椭球体型 ;3.网状结构型。而且 ,对寒武纪和志留纪灰泥丘凝块石作生物标志化合物分析也证明了其有机质生源为菌藻类微生物。这些凝块石灰泥丘中的菌藻类实体化石都显示了不同的沉积、造岩和成丘功能 ,因而证实了微生物在形成古生代灰泥丘中具重要作用。

The natural anti microbial peptides are the anti microbial materials in vivo . The transgenic strain Chjch1 which was constructed in this work was fermented for the purpose of expression of the cationic anti microbial peptide analyzed by Tricine SDS PAGE. It proved the expressed peptide has the right molecular weight. The expressed quantity of peptide,which was up to 29 67?mg/L,was got by the gel analysis system. The max biomass was up to 20 8?g/L when it was fermented in the 250?mL...

The natural anti microbial peptides are the anti microbial materials in vivo . The transgenic strain Chjch1 which was constructed in this work was fermented for the purpose of expression of the cationic anti microbial peptide analyzed by Tricine SDS PAGE. It proved the expressed peptide has the right molecular weight. The expressed quantity of peptide,which was up to 29 67?mg/L,was got by the gel analysis system. The max biomass was up to 20 8?g/L when it was fermented in the 250?mL conical flask. The anti microbial experiments in vitro proved that the recombinant protein could inhibite the growth of Sarcina Subflava Ravenel and Escherichia coli , and their diameters are 2 6?cm and 2 36?cm, respectively.

天然抗菌肽是生物体自身产生的拮抗物质。本实验利用实验室构建的基因工程菌Chjch1诱导表达了阳离子抗菌肽。表达蛋白经Tricine SDSPAGE凝胶电泳分析确定了重组蛋白单体分子质量的正确性。经凝胶成像系统分析确定表达量为 2 9 6 7mg/L。摇瓶发酵菌体最大生物量达 2 0 8g/L。活性实验表明 ,重组蛋白发酵上清液对微黄色八叠球菌和大肠杆菌具有毒杀力 ,30 μL ,96h的发酵上清液对微黄色八叠球菌抑菌直径达 2 6cm ,对大肠杆菌抑菌直径为 2 36cm。

Mud containing oil mainly resulted from the processes of exploration, extraction and transportation of crude oil, and it is intractable for its treatment in the broad oil fields, especially that obtained from the treatment of heating and using solvent that decreased the activities of microbes and therefore more difficult for bioremediation. The influences of applying fertilizer, changing the amount of soil dressing, moisture and pH value on the bioremediation effect of mud containing oil were studied. The experiment...

Mud containing oil mainly resulted from the processes of exploration, extraction and transportation of crude oil, and it is intractable for its treatment in the broad oil fields, especially that obtained from the treatment of heating and using solvent that decreased the activities of microbes and therefore more difficult for bioremediation. The influences of applying fertilizer, changing the amount of soil dressing, moisture and pH value on the bioremediation effect of mud containing oil were studied. The experiment of orthogonal design L934 proved that application of soil dressing was an important factor affecting treatment efficiency. Under natural temperatures, the optimized parameters were as follows: amount of soil dressing 20%, fertilizer 5 %~10%, solid microbial materials 5%, moisture 30% and pH 7. When the original TPH concentrations were in the range of 20.6~22.3g·kg-1, its removal rate was more than 49%. During the composting processes, the contents of O2 in composting piles could reflect the activity of microbe and the treatment efficiency of total petroleum hydrocarbon.

含油污泥主要来源于原油的勘探、开采和运输过程,在回收石油过程中由于采用加热后助剂提取方法,使油泥中大量微生物失活,为生物修复处理带来新的困难。针对这种含油污泥,采取投加固体菌剂的方法增加微生物种群,通过投加客土、肥料、水分及改变pH,调控微生物的营养和生存环境。利用正交实验L934方法选择了较理想的含油污泥生物修复条件。实验结果表明,处理含油污泥时投加客土是影响处理效果的重要因素,较理想的处理条件为:在自然温度条件下,客土投加量10% ̄20%、肥料5%、菌剂5%(湿重)、水分30%(烘干基)和pH7.0。当TPH初始浓度为20.6 ̄22.3g·kg-1时,60d后其去除率>49%。在实验过程中,堆料的O2含量的变化能够反映出生物降解过程中微生物的活性及总石油烃(TPH)的处理效果变化趋势。

 
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