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notch protein
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     protein.
     protein。
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     Conclusion: Notch1 protein is expressed in Raji cells.
     可上调Raji细胞内Notch1蛋白的表达;
短句来源
     The change of expressions of Notch1 protein was tested by flow cytometry.
     可促进细胞内Notch1mRNA的表达,但此表达可部分被雷公藤内酯醇抑制;
短句来源
     RADIOIMMUNOASSAY OF F PROTEIN
     F蛋白的放射免疫分析
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     Progress in Notch Signaling
     Notch信号通路研究进展
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  notch protein
This down-regulation of VEGF in preeclamptic placentas, in turn, probably decreases Notch protein expression in placental compartments and in endothelial cells and could offer an ethiopathogenetic explanation for the onset of this pathology.
      
Distribution of Notch protein members in normal and preeclampsia-complicated placentas
      
The deltex (dx) gene encodes an E3-ubiquitin ligase that binds to the intracellular domain of the Notch protein and regulates Notch signaling in a positive manner.
      
We find that Notch protein specifically accumulates within Dps-expressing cells and appears to be primarily localized within the cytoplasm.
      
Shaggy and Delta are required for the activation of Notch protein.
      
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Notch proteins are involved in cell fate selection throughout development. Signalling through the transmembrane receptor Notch is triggered by ligand binding, which induces the proteolytic cleavage of the Notch protein. This cleavage generates an intracellular fragment of the Notch protein (Notch ICD), which translocates into the nucleus and modifies transcription of target genes through its association with the CSL familily of DNA binding protein (where CSL stands for CBF1,...

Notch proteins are involved in cell fate selection throughout development. Signalling through the transmembrane receptor Notch is triggered by ligand binding, which induces the proteolytic cleavage of the Notch protein. This cleavage generates an intracellular fragment of the Notch protein (Notch ICD), which translocates into the nucleus and modifies transcription of target genes through its association with the CSL familily of DNA binding protein (where CSL stands for CBF1, Su (H), Lag 1). Notch activity affects the implementation of differentiation, proliferation, and apoptotic programs, providing a general developmental tool to influence organ formation and morphogenesis. To obtain recombinant rat NICD, a long template and high fidelity PCR was used to clone NICD (1744V 2530K) DNA fragment from rat brain cDNA library. The cloned NICD fragment was confirmed by sequencing and then subcloned into glutathione S transferase (GST) fusion protein expression vector pGEX KG. The GST NICD fusion proteins were expressed in E.coli JM109 after inducing by IPTG. The fusion proteins were purified by affinity chromatography on glutathione Sepharose 4B.

Notch 1信号途径参与决定细胞命运 ,其水解后产生的活性片段———胞质段 (Notch 1intracellularcytoplasmicdomain ,NICD)能被转运进核 ,激活下游靶基因的表达 .Notch 1参与细胞的增殖、分化、程序性死亡、发育过程中的形态发生和器官形成等许多重要过程 .为了获得重组的NICD ,以大鼠脑cDNA文库为模板 ,用PCR方法扩增出编码NICD的基因片段 ,克隆至谷胱甘肽 S 转移酶 (GST)融合表达载体pGEX KG中 ,并在大肠杆菌中获得较高水平的表达 .表达的融合蛋白GST NICD分子质量约为 12 0ku左右 ,以包涵体和可溶性两种形式存在 ,易于亲合层析纯化 .为制备抗体和进一步的功能研究奠定了基础 .

 
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