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median tissue culture infective dose
相关语句
  半数组织培养感染剂量
     Methods HIV was exposed to different concentrations of ascorbic acid at different temperature. The inactivition result of ascorbic acid on HIV was explored by detecting median tissue culture infective dose (TCID50) at different intervals. The cyto-toxicity of the effective dose on MT4 cell was also explored.
     方法通过测半数组织培养感染剂量(TCID50),检测不同浓度维生素C在不同温度和作用时间下对HIV的灭活作用,并对有效剂量组进行MT4细胞毒性试验。
短句来源
  半数组织培养感染量
     Methods RT-PCR assay was used to detect the mRNA expression of CMV immediate early gene(IE),glyceraldehyde phosphate dehydrogenase(GAPDH) and IL-6 of KM3 cells infected by 100-,10-,1-folds of median tissue culture infective dose(TCID_(50)) of CMV. Flow cytometry was used to detect the expression of pp65 antigen. CMV particles were detected with electron microscope.
     方法 以 10 0 ,10 ,1半数组织培养感染量 (TCID50 )滴度的CMV与KM3细胞共培养 ,RT PCR法检测细胞CMV即刻早期抗原基因 (IE)、甘油醛 3 磷酸脱氢酶基因 (GAPDH)及IL 6mRNA的表达 ,流式细胞术检测细胞CMVpp6 5抗原的表达 ,透射电镜检测细胞内CMV病毒颗粒。
短句来源
  “median tissue culture infective dose”译为未确定词的双语例句
     After ten times continuous dilution,chicken embryo fibroblast (CEF) and chicken embryo were inoculated with pigeon Paramyxovirus Ⅰstrain JS for determining its median tissue culture infective dose(TCID 50 )and 50% egg infectious dose(EID 50 ).
     鸽I型副粘病毒 (PPMV Ⅰ )JS株的尿囊液毒 ,经 1 0倍连续稀释后分别接种鸡成纤维细胞和非免疫鸡胚 ,测定其半数细胞培养感染量 (TCID50 )和鸡胚半数感染量 (EID50 )。
短句来源
     Results (1) The preventive and direct deactivated effects were as follows: The median tissue culture infective dose(TCID_(50)) was lower over 2Log in the Qingfeiyin group than that of the virus control group with a correlation with the decoction concentration.
     结果(1)对流感病毒的预防作用和直接灭活作用:清肺饮组半数组织培养感染(TC ID50)比病毒对照组低2 Log以上,且与药物浓度呈正相关;
短句来源
     The recombinant adenovirus titers were tested with median tissue culture infective dose (TCID50) method.
     B.B DNA。 转染并荧光显微镜观察eGFP的表达,进行重组腺病毒的PCR鉴定,重组腺病毒扩增后氯化铯密度梯度离心法纯化Ad-VEGF_(165),TCID_(50) 50%组织培养感染剂量法,测定腺病毒滴度。
短句来源
     ②The isolated and identified wild-type VZV4 and the standard strain VZV-Ellen were titrated, and the median tissue culture infective dose (TCID50) were calculated.
     ④确定更昔洛韦和阿昔洛韦Vero细胞毒性,分别计算更昔洛韦和阿昔洛韦的半数中毒浓度(median toxic concentration,TC50)和最大无毒浓度(maximal nontoxic concentration,TC0);
短句来源
     The antirotaviral effects of quercetin and 2α-hydroxyursolic acid were studied in vitro by the method of cytopathic effect (CPE), trace median tissue culture infective dose (trace TCID_(50)), and transmission electron microscope (TEM).
     观察细胞病理变化,采用半数组织培养感染(TCID50)微量法、电镜法观察槲皮素和2α-羟基熊果酸体外对HRV的治疗及预防作用。
短句来源
  相似匹配句对
     The median nerve usually degenerates andadheres to the adylcent tissue.
     正中神经受压后常出现神经变性,与周围组织粘连;
短句来源
     Tissue chip
     组织芯片
短句来源
     TISSUE SENSORS
     组织传感器
短句来源
     Methods: Approach to sternum median or thymectomy and peripheral adipose tissue's sweeping.
     方法 :胸骨正中入路或胸腺瘤切除及周围脂肪组织清扫 .
短句来源
     Tissue Culture of Bryophytes
     苔藓植物的组织培养
短句来源
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After ten times continuous dilution,chicken embryo fibroblast (CEF) and chicken embryo were inoculated with pigeon Paramyxovirus Ⅰstrain JS for determining its median tissue culture infective dose(TCID 50 )and 50% egg infectious dose(EID 50 ).Results showed that TCID 50 and EID 50 of strain JS were TCID 50 =10 -9.33 /0.1mL and EID 50 =10 -9.375 /0.1mL,respectively. It suggested that determination data susceptivity of chicken embryo fibroblast (CEF) and chicken...

After ten times continuous dilution,chicken embryo fibroblast (CEF) and chicken embryo were inoculated with pigeon Paramyxovirus Ⅰstrain JS for determining its median tissue culture infective dose(TCID 50 )and 50% egg infectious dose(EID 50 ).Results showed that TCID 50 and EID 50 of strain JS were TCID 50 =10 -9.33 /0.1mL and EID 50 =10 -9.375 /0.1mL,respectively. It suggested that determination data susceptivity of chicken embryo fibroblast (CEF) and chicken embryo were similar.

鸽I型副粘病毒 (PPMV Ⅰ )JS株的尿囊液毒 ,经 1 0倍连续稀释后分别接种鸡成纤维细胞和非免疫鸡胚 ,测定其半数细胞培养感染量 (TCID50 )和鸡胚半数感染量 (EID50 )。结果显示 ,JS株TCID50 为 1 0 - 9.33/0 .1mL ,EID50 为 1 0 - 9.375/0 .1mL ,表明JS株病毒对鸡胚和鸡胚成纤维细胞的易感性相近。

Objective To study the inhibition of respiratory syncytial virus (RSV) by Ara-AMP,virazole,bupleu-rum root injection (bupleurum) and their joint application.Methods Ara-AMP,virazole and bupleurum were diluted to dif-ferent concentrations,and the cell poison reaction by drug cytotoxicity was observed by cell culture;cytopathic effect (CPE) and RSV inhibition by drugs were observed by the way of drug administration two hours after Vero cells were infected by virus. Re-sults The toxic dosages (TD) to Vero cells...

Objective To study the inhibition of respiratory syncytial virus (RSV) by Ara-AMP,virazole,bupleu-rum root injection (bupleurum) and their joint application.Methods Ara-AMP,virazole and bupleurum were diluted to dif-ferent concentrations,and the cell poison reaction by drug cytotoxicity was observed by cell culture;cytopathic effect (CPE) and RSV inhibition by drugs were observed by the way of drug administration two hours after Vero cells were infected by virus. Re-sults The toxic dosages (TD) to Vero cells of Ara-AMP,virazole and bupleurum were 0.25,0.2 and 2 mg/ml,respectively. The drug cytotoxicity was not enhanced when Ara-AMP or virazole combined with bupleurum,but it was done when Ara-AMP combined with virazole.Each of the three drugs inhibited RSV in Vero cells,and the effective dosage of Ara-AMP and virazole was both 25 μg/ml,and bupleurum was 125 μg/ml.The combinations of 3 drugs showed synergism without an-tagonism.After Vero cells were infected by RSV 100 TCID 50 /0.1 ml(ie. median tissue culture infective dose),the results showed RSV was inhibited significantly when Ara-AMP 125 μg/ml,virazole 100 μg/ml and bupleurum 1 000 μg/ml were given to Vero cells tubes in the time of Vero cells just being infected and having been infected for 2 h and 12 h;the inhibition effects were reduced when drugs were given after Vero cells had been infected for 24 h;and no inhibition effects were showed after 36 h.Conclusion RSV could be inhibited by Ara-AMP,virazole and bupleurum,respectively.Significant synergism against RSV was showed by their joint application,this not only can decrease the dosage,toxicity,but also enhance the inhibition ef-fect on RSV obviously.

目的研究单磷酸阿糖腺苷(Ara-AMP)、病毒唑、柴胡及其联合应用对呼吸道合胞病毒(RSV)的抑制作用。方法将Ara-AMP、病毒唑及柴胡分别稀释成不同的浓度,用细胞培养法观察药物的细胞毒性反应;采用Vero细胞先感染病毒2h后给药法观察细胞病变(CPE)和药物对RSV的抑制作用。结果Ara-AMP、病毒唑及柴胡对Vero细胞的毒性剂量(TD)分别为0.25、0.2、2mg/ml;当Ara-AMP、病毒唑分别与柴胡联用时并未增加对细胞的毒性,而Ara-AMP与病毒唑联用时则对细胞的毒性增加;3种药物各自在Vero细胞上对RSV都有抑制作用,Ara-AMP和病毒唑的有效剂量均为25滋g/ml,柴胡为125滋g/ml。当3种药物配伍联合应用时,均可产生协同作用。结论Ara-AMP、病毒唑及柴胡各自对RSV具有显著的抑制作用,它们的联合应用可产生显著协同作用,既可降低用药量,减少毒副反应,又可有效抑制RSV的感染。

Objective In order to explore the inactivating effect of ascorbic acid on human immunodeficiency virus (HIV) and provide a safe and practical approach to inactivating HIV in biological products. Methods HIV was exposed to different concentrations of ascorbic acid at different temperature. The inactivition result of ascorbic acid on HIV was explored by detecting median tissue culture infective dose (TCID50) at different intervals. The cyto-toxicity of the effective dose on MT4 cell was also explored....

Objective In order to explore the inactivating effect of ascorbic acid on human immunodeficiency virus (HIV) and provide a safe and practical approach to inactivating HIV in biological products. Methods HIV was exposed to different concentrations of ascorbic acid at different temperature. The inactivition result of ascorbic acid on HIV was explored by detecting median tissue culture infective dose (TCID50) at different intervals. The cyto-toxicity of the effective dose on MT4 cell was also explored. Results 500 μg/ml vitamin C can inactivate HIV in serum-free liquid, whereas this dose was toxic to MT4 cell irreversibly. Conclusion ascorbic acid does not suit inactivating HIV in blood or blood products.

目的验证维生素C对人类免疫缺陷病毒(HIV)的灭活作用,为生物制品提供一种安全可行的HIV灭活的方法。方法通过测半数组织培养感染剂量(TCID50),检测不同浓度维生素C在不同温度和作用时间下对HIV的灭活作用,并对有效剂量组进行MT4细胞毒性试验。结果500μg/ml维生素C在无血清状态下有灭活HIV作用,而此剂量对MT4细胞有不可逆转的毒性作用。结论维生素C不适宜用于血及血制品的HIV灭活。

 
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