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feathery mottle
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  羽状斑驳
     The result showed that there were sweet potato feathery mottle virus (SPFMV) and sweet potato latent virus (SPLV) commonly in the samples, but it was not sure if there were sweet potato mild mottle virus (SPMMV) and sweet potato caulimo-like virus (SPCLV) in the samples.
     结果表明:上述地区甘薯中普遍存在甘薯羽状斑驳病毒(SPFMV)和甘薯潜隐病毒(SPLV),尚难确定是否存在甘薯轻斑驳病毒(SPMMV)和甘薯花叶菜花叶状病毒(Sweet Potato Caulimo-like Virus,SPCLV)。
短句来源
     Determination and analysis of 3′-terminal genomic sequence of Sweet potato feathery mottle virus
     甘薯羽状斑驳病毒分离物的基因组3′-末端序列测定与分析
短句来源
     Sweet Potato Virus G usually infects Sweet Potato with Sweet Potato feathery mottle virus and some other potyvirus. As a result it severly destroies the production of sweet potato ,for example the reduction of production and quality of sweet potato and degradation of variety.
     甘薯病毒G(Sweet Potato Virus G,SPVG)是侵染甘薯的主要病毒之一,其常常与甘薯羽状斑驳病毒(SPFMV)等其他potyvirus属病毒混合侵染,导致甘薯产量降低、品质变劣和种性退化,对甘薯生产造成严重危害。
短句来源
     According to published nucleotide sequence, coat protein gene of sweet potato feathery mottle virus (SPFMV) isolated from China was synthesized by reverse transcripation-polymerase chain reaction (RT- PCR ) and constructed into expression vector pET30a (+) for overexpression in prokaryotic cells.
     根据已报道的甘薯羽状斑驳病毒(SPFMV)外壳蛋白(CP)基因的核苷酸序列合成引物,利用RT-PCR的方法获得CP基因后,再将其克隆到原核表达载体pET30a(+)中。
短句来源
     weet potato feathery mottle virus(SPFMV)was inoculated to I. selosa to propagare by graftingand was purified wirh 0.2mol/L pH7.2 PBK. Further purification of SPFMV was conducted by sucrose cushion and sucrose density gradient centrifugation.
     用嫁接方法将甘薯羽状斑驳病毒(SPFMV)接种到I.setosa上扩繁,以0.2mol/LpH7.2PBK缓冲液、垫层差速离心、蔗糖密度梯度离心提取纯化SPFMV。
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  “feathery mottle”译为未确定词的双语例句
     Preparation of Monoclonal Antibody to Sweet Potato Feathery Mottle Virus
     甘薯羽状斑驳病毒单克隆抗体研制成功
短句来源
     IDENTIFICATION OF LANGFANG ISOLATE OF SWEET POTATO FEATHERY MOTTLE VIRUS
     甘薯病毒病的研究Ⅰ甘薯羽状斑驳病毒的分离、鉴定
短句来源
     Purification and Serology of Sweet Potato Feathery Mottle Virus
     甘薯羽状斑驳病毒分离提纯及血清学特性
短句来源
     ISOLATION, PURIFICATION OF SWEET POTATO FEATHERY MOTTLE VIRUS
     甘薯羽状斑驳病毒的分离与提纯
短句来源
     Isolation and Purification of Sweet Potato Feathery Mottle Virus
     甘薯羽状斑驳病毒的分离和提纯
短句来源
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  相似匹配句对
     ISOLATION, PURIFICATION OF SWEET POTATO FEATHERY MOTTLE VIRUS
     甘薯羽状斑驳病毒的分离与提纯
短句来源
     Purification and Serology of Sweet Potato Feathery Mottle Virus
     甘薯羽状斑驳病毒分离提纯及血清学特性
短句来源
     IDENTIFICATION OF PEANUT MOTTLE VIRUS IN CHINA
     花生斑驳病毒的鉴定
短句来源
     The Cause of print mottle and the methed of prevention
     印刷“墨斑”成因分析与控制
短句来源
     Observation and analysis of feathery austenite of hypoeutectoid steel
     亚共析钢羽毛状奥氏体观察与分析
短句来源
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  feathery mottle
Several potyviruses are found infecting sweet potato (Ipomoea batatas) in Peru, of which sweet potato feathery mottle virus (SPFMV, genus Potyvirus) is the most common.
      
Molecular variability of sweet potato feathery mottle virus and other potyviruses infecting sweet potato in Peru
      
Sweet potato feathery mottle virus (SPFMV, genus Potyvirus) infects sweet potatoes (Ipomoea batatas) worldwide, but no sequence data on isolates from Africa are available.
      
Comparisons of coat protein gene sequences show that East African isolates of Sweet potato feathery mottle virus form a genetica
      
The coat protein (CP) genes of the genomic RNA of two Korean isolates of sweet potato feathery mottle potyvirus (SPFMV), SPFMV-K1 and SPFMV-K2, were cloned and their complete nucleotide sequences were determined.
      
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253 entries of sweet potato diseased samples from four provinces (city) of Jiangsu, Shandong, Sichuan and Beijing were detected with four antibodies (IgG) to sweet potato viruses and by three serological methods. The result showed that there were sweet potato feathery mottle virus (SPFMV) and sweet potato latent virus (SPLV) commonly in the samples, but it was not sure if there were sweet potato mild mottle virus (SPMMV) and sweet potato caulimo-like virus (SPCLV) in the samples. It was believed...

253 entries of sweet potato diseased samples from four provinces (city) of Jiangsu, Shandong, Sichuan and Beijing were detected with four antibodies (IgG) to sweet potato viruses and by three serological methods. The result showed that there were sweet potato feathery mottle virus (SPFMV) and sweet potato latent virus (SPLV) commonly in the samples, but it was not sure if there were sweet potato mild mottle virus (SPMMV) and sweet potato caulimo-like virus (SPCLV) in the samples. It was believed that there were other viruses on sweet potato in China since 21% samples with symptoms had.no reaction with tested antisera. The accuracy and sensitivity of Dot blot-ELISA and ISEM were better than that of DAS-ELISA for detection of viruses on sweet potato.

作者用4种甘薯病毒抗体(IgG),3种血清学方法(DAS-ELISA、Dot-blot-ELISA和ISEM)对北京,江苏、四川、山东四省(市)的253份甘薯病毒病样品进行了检测。结果表明:上述地区甘薯中普遍存在甘薯羽状斑驳病毒(SPFMV)和甘薯潜隐病毒(SPLV),尚难确定是否存在甘薯轻斑驳病毒(SPMMV)和甘薯花叶菜花叶状病毒(Sweet Potato Caulimo-like Virus,SPCLV)。21%的显症样品同上述4种病毒的抗血清不产生反应,显示我国甘薯上尚存在其它病毒。用Dot blot-ELISA和ISEM检测甘薯病毒比用DAS-ELISA灵敏准确。

This paper describes the preliminary research results on the host range,aphid transmission,particle morphology and serology of Langfang isoloateof sweet potato feathery mottle virus(SPFMV-LF) obtained by grafting andthe results on the comparison with SPFMV-KC(from USA) SPFMV-LF,which was 800-830nm in length,readily both sap and grafttransmissible.It was also transmitted by Aphis gossypii Glover and Myzus persicae Sulzer.It infected Ipomoea setosa,I.nil systematically,but did not infect Chenopo-dium quinoa,C.amaranticolor,Nicotiana...

This paper describes the preliminary research results on the host range,aphid transmission,particle morphology and serology of Langfang isoloateof sweet potato feathery mottle virus(SPFMV-LF) obtained by grafting andthe results on the comparison with SPFMV-KC(from USA) SPFMV-LF,which was 800-830nm in length,readily both sap and grafttransmissible.It was also transmitted by Aphis gossypii Glover and Myzus persicae Sulzer.It infected Ipomoea setosa,I.nil systematically,but did not infect Chenopo-dium quinoa,C.amaranticolor,Nicotiana benthamiana and other species of toba-cco.The local lesion host of this virus has not been found.The symptoms onI.setosa infected with SPFMV-LF and SPFMV-RC were similar,but differenton I.nil.Ascites antibody to SPFMV-LF was produced in BALB/c miceimmunized with virus preparation purified from SPFMV-LF infected I.setosaafter twice high speed centrifugation in sucrose cushion.Immuno-electronmicroscopy revealed that the antibody reacted with SPFMV-RC strain SoSPFMV-LF obtained in this test was a strain of SPFMV,but different fromSPFMV-RC.

本文从寄主范旧,蚜传特性、病毒颗粒形态、免疫电镜等方面对嫁接获得的甘薯羽状斑驳病毒廊坊分离物(SPFMV—LF)进行了鉴定,并和SPFMV—RC(美国株)进行了实验比较。SPFMV—LF易汁液摩擦传播和嫁接传播,并以蚜虫非持久性传播,SPFMV—LF系统侵染巴西牵牛(Ipomoea setosa)、牵牛(I.nil);不侵染Chenopodium quinoa、C.ama-ranticolor、Nicotiana benthamiana及其它烟草。尚未发现其局部斑寄主。SPFMV—LF及SPFMV—RC感染I.setosa后引起相似的症状,但感染I.nil后症状差别较大。病毒颗粒长860—830nm,经两次蔗糖垫超速离心从SPFMV—LF感染的I.setosa叶片提取病毒并在BALB/C小鼠中制备抗SPFMV—LF的腹水多克隆抗体,免疫电镜表明,SPFMV—LF的抗体和SPFMV—RC株系有反应。上述实验结果表明SPFMV—LE是SPFMV的一个株系,但不同于SPFMV—RC。

Sweet potato feathery mottle virus (SPEMV) was purified from infected I.Nil leaves using ultracentrifugation and CsCl step gradient centrifugations. Virus yields of 13—15 mg Per kilogram of infected Ieaf tissue were obtained. The normal particle lengths of the SPFMV was 820—860nm. The virus obtained was highly infectious and a good immunogen. Purified virus had a OD260/OD280 ratio of 1.21.

从感染有甘薯羽状斑驳病毒的牵牛(Ⅰ.Nil)叶片,通过超速离心,Cscl密度梯度离心提纯SPFMV粒子,每千克叶片可得病毒13—15毫克。电镜观察病毒粒子长度范围在820—860nm,也可见到900nm以上的特长粒子。病毒提纯物的紫外吸收曲线呈典型核蛋白吸收曲线,OD260/OD280=1.21,免疫电镜检查,该病毒与SPFMV抗血清起阳性反应。

 
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