6 With the plant hormone is connected with organic accretion orthogonal design test, being fit for Virginia Creeper reproduces quickly optimum culture medium: 1/2 MS +NAA 0.04 mg/L + BA 1.0 mg/L + KT 2.0 mg/L + LH 100 mg/L + white sugar 30 g/L + agar 7.5 g/L;
The optimum explant of Virginia Creeper to adduce callus is the stalk passage, the culture medium constitutes: 1/2 MS + 2,4-D 0.8 mg/L + BA 0.5 mg/L + white sugar 30 g/L + agar 7.5 g/L, the callus is light green, the quality of the callus is loose , differentiatia frequency higher .
2 The optimum explant of Silvervein Creeper to adduction callus is the petiole, the main adduction hormone is NAA ,culture medium : 1/2 MS + NAA 0.1 mg/L + BA 0.5 mg/L + white sugar 30 g/L + agar 7.5g/L, adduced callus by it more early, quantity far more , the greener, differentiatia frequency higher;
3 In differentiatia and subculture stage, Silvervein Creeper easy brown, and attachs PVP 300 mg/L alleviate the brownization degree. The fundamental culture medium of more fit growth of Silvervein Creeper is 1/2MS and GS, that attachs BA 0.5 mg/L, and NAA 0.06 mg/L;
5 The Virginia Creeper is very vast as to the accommodation of pH, and is living in the environment of pH 4.6-6.4 wholly to grow, the most fit limit is pH 5.4-6.2 where the seeding grows robustly, and stalk extending amount is greatly;
The research covered two aspects: one was the interspecific hybridization technology of Boston ivy(Parthenocissus tricuspidata) and Virginia Creeper (Parthenocissus quinqeufolia) and the analysisof their hybrid obstacle.