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viral capsid protein
相关语句
  病毒衣壳蛋白
     Porcine circovirus type 2(PCV2) genome contains two major open reading frames(ORFs),ORF1 encoding the viral replication-associated protein(Rep) and ORF2 encoding the viral capsid protein(Cap).
     猪圆环病毒2型(PCV2)基因组包含2个开放阅读框架(ORFs),其中ORF1编码病毒复制相关蛋白(Rep),ORF2编码病毒衣壳蛋白(Cap)。
短句来源
     IR-BG-infected WB cells. This indicated that selective activation of viral capsid protein synthesis in tumor cells could be one of the determinants for Ad IR-BG-mediated selective oncolysis.
     说明肿瘤细胞和正常细胞对病毒衣壳蛋白合成的激活存在差别,可能是Ad.IR-BG在肿瘤细胞中选择性地复制的原因之一。
短句来源
  “viral capsid protein”译为未确定词的双语例句
     They commonly shared the replication initiator protein (Rep) RNA and the viral capsid protein (CR) RNA. CHN-2A had more abundant Rep but shorter CR expression than CHN-2H. Moreover, CHN-2A had the other RNA (designated AD and Reps), but CHN-2H had none.
     两者都具有病毒复制酶蛋白RNA(Rep)和病毒核衣壳蛋白RNA(CR),但CHN-2A(BF)的Rep表达量大,CHN-2H的CR表达时间长。
短句来源
     Five synthetic peptides (EIA-1, EIA-2, EIA-3, EIA-4 and EIA-5) were synthesized according to sequences selected from the viral capsid protein (C) and nonstructural protein regions.
     选择丙型肝炎病毒的核衣壳(C)区及非结构蛋白(NS)区,分别合成了5段肽(EIA-1、EIA-2、EIA-3、EIA-4和EIA-5)。
短句来源
     The other is the C-terminal PTK motif,which contributes to binding of HC-Pro to the viral capsid protein’sN-terminal DAG motif.
     本研究缺失HC-Pro的蚜传必需的区域,将其导入烟草中表达从而与野生型HC-Pro竞争以抑制蚜虫传毒的效率。
短句来源
     RNA as template of RT-PCR was extracted from fecal supernatant containing the swCH25 strain of Hepatitis E virus, and then the C-terminal 916bp of viral capsid protein (ORF2) gene was amplified.
     以RT-nPCR扩增戊型肝炎病毒猪源株swCH25 ORF2基因片断,并将其插入到pMD18-T simple载体中,构建克隆载体pMD-ORF2。
短句来源
     The mouse-adapted Lansing strain of poliovirus type 2 PV-2(L) induces fatal poliomyelitis in mice after intracerebral inoculation, while mice inoculated with Mahoney strain of poliovirus type 1 PV-1(M) show no signs of disease. Previous work had indicated that both the 5' non-transslated region of the viral genome and the viral capsid protein, neutralization antigenic site Ⅰ(N-Agl), were involved in mouse neurovirulence.
     为探讨脊髓灰质炎病毒Ⅱ型Lansing株中和抗原位点1(N-Ag1)在对小鼠适应能力和神经毒力中的意义和作用,本实验采用DNA重组技术构建的2株抗原嵌合性(Ⅰ/Ⅱ型)PVXF414和XF324,对小鼠适应性和神经毒力进行研究。
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  相似匹配句对
     Study of Dengue Virus Capsid Targeted Viral Inactivation
     登革病毒衣壳蛋白靶向抗病毒作用的研究
短句来源
     Capsid-targeted viral inactivation for dengue virus infection
     衣壳蛋白靶向灭活策略应用于抗登革病毒感染的研究
短句来源
     Viral Hepatitis and Typhoid
     肝炎和伤寒流行过程的混沌分维研究
短句来源
     Viral infections in pigeons
     鸽的病毒性感染
短句来源
     Display of Viral Structural Protein VP1 of FMDV on the SOC Site of Bacteriophage T4 Capsid Surface
     O型FMDV结构蛋白VP1在T4噬菌体表面的展示(英文)
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  viral capsid protein
Full length HAV RNA and viral capsid protein VP1 were detected in 18f infected cells at earlier times post-infection than in HM175/clone 1 infected cells.
      
The viral capsid protein (CP) cistron is located at the 5' terminal end of RNA2 and the Mr of CP (20?K) is close to that determined by SDS-PAGE analysis.
      
A 75-kDa major protein and a 160-kDa minor protein were detected by anti-CAP serum in a TVV-T1 sample, indicating that the 75-kDa protein is the viral capsid protein.
      
The gene for the viral capsid protein was shown to reside in the 3' terminal half of the genomic RNA.
      
In the early stage of infection, both 155K and 110K viral proteins were associated with the nuclear matrix, while in the late stage, 155K protein, presumably a viral capsid protein, was predominantly associated with the matrix.
      
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Five synthetic peptides (EIA-1, EIA-2, EIA-3, EIA-4 and EIA-5) were synthesized according to sequences selected from the viral capsid protein (C) and nonstructural protein regions. Out of 158 anti-HCV positive sera (screened by Abbott 2nd generation kits), 105 were positive for anti-peptide antibodies; among which, antibodies versus EIA-2 and EIA-5 positive rates were the highest, being 89% and 85% respectively. Ameng 62 anti-HCV negative sera, 12 were positive for anti-peptide antibodies and among...

Five synthetic peptides (EIA-1, EIA-2, EIA-3, EIA-4 and EIA-5) were synthesized according to sequences selected from the viral capsid protein (C) and nonstructural protein regions. Out of 158 anti-HCV positive sera (screened by Abbott 2nd generation kits), 105 were positive for anti-peptide antibodies; among which, antibodies versus EIA-2 and EIA-5 positive rates were the highest, being 89% and 85% respectively. Ameng 62 anti-HCV negative sera, 12 were positive for anti-peptide antibodies and among them 6 sera showed elevated ALT. It is highly suggestive that these 6 sera were from HCV infected individuals. The principles for selection of peptide sequences for mimicking antigenic epitopes and their prospects for future use are discussed.

选择丙型肝炎病毒的核衣壳(C)区及非结构蛋白(NS)区,分别合成了5段肽(EIA-1、EIA-2、EIA-3、EIA-4和EIA-5)。158份抗-HCV阳性血清中,105份(66.5%)有对合成肽的抗体。以对EIA-2及EIA-5抗体的阳性率较高,分别占阳性总数的89%和85%。62份抗-HCV阴性血清中,12份有合成肽抗体,其中6份血清转氨酶升高,提示为丙型肝炎感染者血清。对选择合成肽段及作为抗原决定簇研究和应用的前景进行了讨论。

The mouse-adapted Lansing strain of poliovirus type 2 PV-2(L) induces fatal poliomyelitis in mice after intracerebral inoculation, while mice inoculated with Mahoney strain of poliovirus type 1 PV-1(M) show no signs of disease. Previous work had indicated that both the 5' non-transslated region of the viral genome and the viral capsid protein, neutralization antigenic site Ⅰ(N-Agl), were involved in mouse neurovirulence. In order to further explore the role of N-Agl in mouse neurovirulence, antigenic chimeras...

The mouse-adapted Lansing strain of poliovirus type 2 PV-2(L) induces fatal poliomyelitis in mice after intracerebral inoculation, while mice inoculated with Mahoney strain of poliovirus type 1 PV-1(M) show no signs of disease. Previous work had indicated that both the 5' non-transslated region of the viral genome and the viral capsid protein, neutralization antigenic site Ⅰ(N-Agl), were involved in mouse neurovirulence. In order to further explore the role of N-Agl in mouse neurovirulence, antigenic chimeras of two poliovirus strains, XF414 and XF324, were constructed. In the two strains, ten amino acids (in XF414) or 16 amino acids(in XF324)in antigenic site I in Vpl of PV-I (M) were replaced with a PV-2(L)-specific sequences using a mutagenesis cartridge. Mouse neurovirulence tests indicated that mice cerebrally inoculated with XF414 and XF324 developed poliomyelitis leading to paralysis or death. The viruses possessing antigenicity of the inoculating viruses were isolated from cerebral tissues of the paralyzed mice. The results demonstrated that N-Agl is an important determinant of poliovirus host range, and may be involved in attachment and penetration of poliovirus (in)to cells of the mouse centrol nervous system.

为探讨脊髓灰质炎病毒Ⅱ型Lansing株中和抗原位点1(N-Ag1)在对小鼠适应能力和神经毒力中的意义和作用,本实验采用DNA重组技术构建的2株抗原嵌合性(Ⅰ/Ⅱ型)PVXF414和XF324,对小鼠适应性和神经毒力进行研究。证明Ⅰ型Mahoney株中的N-Ag1被LansingⅡ型毒株的N-Ag1肽段取代后,形成的抗原嵌合性PV Ⅰ/Ⅱ型毒株脑内接种能引起小鼠中枢神经系统脊髓灰质炎病变,导致四肢麻痹或死亡。从麻痹小鼠大脑组织中分离到与接种病毒抗原性相同的活病毒,表明N-Ag1上这一小段氨基酸序列在决定PV宿主适应性中起重要作用;N-Ag1可能与病毒吸附和穿入小鼠中枢神经组织细胞有关。

Soft-ionization mass spectrometry is a kind of newly-developed technology, which mainly refers to Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) and Electrospray Mass Spectrometry (ESI-MS). Bearing some valuable features, such as nondestructive to samples, high mass detection limits, ability to accurately measure molecule weights, and tolerant to complicated samples, they are ideal candidates for analyzing microorganisms. MALDI-TOF-MS, combined with high-resolution...

Soft-ionization mass spectrometry is a kind of newly-developed technology, which mainly refers to Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) and Electrospray Mass Spectrometry (ESI-MS). Bearing some valuable features, such as nondestructive to samples, high mass detection limits, ability to accurately measure molecule weights, and tolerant to complicated samples, they are ideal candidates for analyzing microorganisms. MALDI-TOF-MS, combined with high-resolution 2D-SDS-PAGE gel electrophoresis, offer microbiologists powerful tools for bacterial proteome research. In systematic bacteriological realm, MALDI-TOF-MS and HPLC-ESI-MS can generate "fingerprinting" mass spectrums from intact bacterial cells, which will facilitate the identification, differentiation, typing, and detection of bacterium. Some researchers have successfully utilized MALDI-TOF-MS and ESI-MS to the studying of viral capsid proteins, including measuring molecule weights, exploring dynamic properties, characterizing post-translational modifications and mutations. With the developments of MS technology and the improvements of instruments' performance, more and more microbiologists will recognize MALDI-TOF-MS and ESI-MS as their promising and versatile tools for daily research works.

包括基质辅助激光解吸电离 (MALDI)和电喷雾 (ESI)在内的软电离质谱是最近发展起来的质谱技术 ,由于这些电离方式对样品的破坏性小 ,质量测定范围大 ,分子量测定准确 ,样品纯度要求不高 ,很适合分析成分复杂的微生物样品。MALDI TOF MS结合高分辨率的二维SDS PAGE可以分析 10 - 12摩尔水平的蛋白 ,是细菌蛋白质组研究过程中必不可少的工具。最近的研究工作表明 ,通过MALDI TOF MS或HPLC ESI MS获得的细菌的全细胞质谱指纹图可以作为细菌分类鉴定以及检测的新指标。由于病毒的组成相对较为简单 ,最适合用软电离质谱进行研究 ,MALDI TOF MS和ESI MS已被成功地用于病毒衣壳蛋白相对分子质量测定、动力学特性、转录后修饰以及突变等方面的研究。相信随着技术的进一步发展 ,软电离质谱将在微生物学领域得到更为广泛的应用

 
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