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tissue culture infective dose
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  组织培养感染量
     Methods RT-PCR assay was used to detect the mRNA expression of CMV immediate early gene(IE),glyceraldehyde phosphate dehydrogenase(GAPDH) and IL-6 of KM3 cells infected by 100-,10-,1-folds of median tissue culture infective dose(TCID_(50)) of CMV. Flow cytometry was used to detect the expression of pp65 antigen. CMV particles were detected with electron microscope.
     方法 以 10 0 ,10 ,1半数组织培养感染量 (TCID50 )滴度的CMV与KM3细胞共培养 ,RT PCR法检测细胞CMV即刻早期抗原基因 (IE)、甘油醛 3 磷酸脱氢酶基因 (GAPDH)及IL 6mRNA的表达 ,流式细胞术检测细胞CMVpp6 5抗原的表达 ,透射电镜检测细胞内CMV病毒颗粒。
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     [WTHZ]METHODS: Test 50% percent tissue culture infective dose (TCID_ 50) by cytopathetic effect (CPE) and plaque methods.
     方法:通过测定细胞病变效应(CPE)的半定量法及噬斑定量法测定组织培养感染量(TCID50); 以PEG沉淀法部分纯化病毒,利用免疫学方法免疫动物制备抗MHVA59的多克隆抗体;
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  “tissue culture infective dose”译为未确定词的双语例句
     The Szg and Ssq strains had clear cytopathic effect(CPE) on Vero cell, and the 50% tissue culture infective dose were TCID_(50)=10~(-3.925)/0.5ml, TCID_(50)=10~(-3.223)/0.5ml, respectively. Spherical and capsular virus particles were observed in electron microscope in Vero-cultured virus.
     Ssq株和Szg株在Vero细胞上的细胞病变(CPE)明显,对细胞的感染毒力分别为TCID_(50)=10~(-3.925)/0.5ml和TCID_(50)=10~(-3.223)/0.5ml。
短句来源
     Examine the 50% embryo lethal dose (ELD50) of the strain GX8/99 and the cell-cloned virus separately, and examine the mean tissue culture infective dose (TCID50 ) of the cell-cloned virus .
     对超强毒IBDV GX8/99株原始毒囊毒及其克隆化细胞毒进行ELD50测定,并对克隆化细胞毒进行TCID50测定。
短句来源
     The rescued virus R- A/Chicken/Guangdong/04(R-CG) and the wild type A/Chicken/Guangdong/04(W-CG) shared similar biological properties such as in titers of 50% embryo infective dose(EID50), 50% tissue culture infective dose(TCID50) and intravenous pathogenecity index(IPVI).
     实验通过反向遗传操作技术对该病毒进行拯救,获得了拯救毒R-A/Chicken/Guangdong/04(R-CG)。 R-CG与其亲本毒A/Chicken/Guangdong/04(W-CG)在胚半数感染量(EID50)、细胞培养半数感染量(TCID50)、对SPF鸡和BALB/c小鼠致病力等生物学特性保持一致。
短句来源
     Compared with that in control cells,the anti-IBRS_(2) cells line was able to delay virus-induced cell death by 21 or 27h at an 50% tissue culture infective dose of 2×10~(3)/mL or 4×10~(2)/mL.
     用2×103和4×102TCID50/mL剂量的TGEV感染时,引起细胞死亡的时间分别为21 h和27 h,与对照组相比,抗性细胞系可明显延迟因病毒感染引起细胞死亡的时间。
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     After NDV were inoculated for 72 h,the 50% tissue culture infective dose(TCID 50 )of NDV Ⅰ strain and hemagglutination titer of NDV Ⅳ strain were determined to evaluate the effects of the three components on cellular infectivity of the virus.
     于病毒接种后 72h测定NDVⅠ系的半数组织培养感染剂量 (TCID50 )和NDVⅣ系的血凝效价 ,以评价 3种中药成分对NDV感染细胞的影响。
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  相似匹配句对
     TISSUE CULTURE OF CUCUMBER
     黄瓜的组织培养
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     Tissue Culture of Gypsophila
     满天星的组织培养
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     Culture
     文化艺术
短句来源
     Culture
     文化(英文)
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     Infective Massive Hemobilia
     感染性胆道大出血
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  tissue culture infective dose
Maximum virus yields in MARC-145, P, and L-1 cell clones were 108.5, 103.5, and 102.5 tissue culture infective dose 50 (TCID50)/0.1 ml, respectively.
      
Time-kill studies were carried out, and the virus titer was determined based on the 50% tissue culture infective dose (TCID50).
      
Only the Lepidopteran cell lines supported replication of the virus with TN-CL1 and BCIRL-HZ-AM1 producing the highest titers of 2.4?×?108 tissue culture infective dose (TCID)50/ml and 2.0?×?108?TCID50/ml, respectively.
      
punctigera cell line (HP1) replicated AcMNPV to the highest titer (1.0×108 50% tissue culture infective dose/ml), and only one of the H.
      
The data represent the mean SEM of two independent experiments and are presented as tissue culture infective dose/106 thymocytes.
      
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To establish a rapid diagnostic method for enteroviral infections in clinic, the authors developed a reverse transcription and polymerase chain reaction (RT PCR) assay. Primers homologous to the conserved 5′ noncoding region were designed by analysis of enteroviral genomes, and then were used to enzymatically amplify enterovirus (EV) specific RNA fragment from 31 prototype enteroviral strains and cerebrospinal fluid (CSF) of 34 patients with aseptic meningitis and 11 patients with encephalitis of unknown...

To establish a rapid diagnostic method for enteroviral infections in clinic, the authors developed a reverse transcription and polymerase chain reaction (RT PCR) assay. Primers homologous to the conserved 5′ noncoding region were designed by analysis of enteroviral genomes, and then were used to enzymatically amplify enterovirus (EV) specific RNA fragment from 31 prototype enteroviral strains and cerebrospinal fluid (CSF) of 34 patients with aseptic meningitis and 11 patients with encephalitis of unknown etiology. The amplified products were detected by agar gel electrophoresis and slot blot hybridization analysis. The study on sensitivity of the RT PCR showed that the amplification could detect EV RNA from 10 -2 ~10 -3 50% tissue culture infective doses of the cultured viral strains. The results also showed that 21 (61.8%) of 34 aseptic meningitis cases and 8 (72.7%) of 11 encephalitis cases were positive for EV RNA in CSF samples. Two meningitis and one encephalitis cases were positive during convalescence. These results suggested that this RT PCR method was rapid, sensitive and specific in detection of most common EV infections.

为建立一种能检测出临床上绝大多数肠道病毒(EV)感染的快速、敏感、特异的逆转录聚合酶链反应(RT-PCR)方法,通过对EV基因组的序列分析,在其5′端非编码区设计一对引物,用该引物建立的RT-PCR方法对31种EV标准毒株和34例无菌性脑膜炎及11例无菌性脑炎患儿的脑脊液(CSF)分别进行扩增,其产物做琼脂糖凝胶电泳及斑点杂交检测。研究表明,31种EV标准毒株斑点杂交的敏感度达10-2~10-350%组织培养感染量(TCID50)。34例无菌性脑膜炎中21例阳性(61.8%),11例无菌性脑炎中8例阳性(72.7%);阳性患儿恢复期CSF,2例无菌性脑膜炎和1例无菌性脑炎仍为阳性。提示用RT-PCR方法检测EV感染是值得推广的。

A multivalent fluorescent antibodies preparation was produced for the detection of exogenous viruses contamination in non-avian cells. This is a mixture of a hexavalent fluorescent antibodies preparation and a monovalent preparation. The former antiserum was produced by pigs hyperimmunized wiht the hog cholera virus (HCV) , porcine parvovirus (PPV) , bovine viral diarrhea-mucosal disease virus(BVD/MDV),pseudorabies virus(PrV), bluetonge virus (BtV) and orf virus (OV) , the later ...

A multivalent fluorescent antibodies preparation was produced for the detection of exogenous viruses contamination in non-avian cells. This is a mixture of a hexavalent fluorescent antibodies preparation and a monovalent preparation. The former antiserum was produced by pigs hyperimmunized wiht the hog cholera virus (HCV) , porcine parvovirus (PPV) , bovine viral diarrhea-mucosal disease virus(BVD/MDV),pseudorabies virus(PrV), bluetonge virus (BtV) and orf virus (OV) , the later one by rabbits hyperimmunized wiht rabies virus (RV) . And each of these two was conjugated with fluorescent isothiocyanate (FITC) separately and then mixtured in an adequate ratio. Under the fluorescent microscopic adamination, every given contaminating virus plaque would be stained in a characteristic feature, on the basis of this character, the exogenous contaminator would be identified. Each of these virus specific fliorescene feature would be inhibited by its specific antiserum in the staining inhibition test. The 50% tissue culture infective dose (TCID50) tested by the fluorescent antibody stain method was higher than that by the routine method.80 samples of cells which produced disruptions in producing live hog cholera vaccine obtained from biologics factories were assayed with this preparation.13 and 12 of them were demonstrated to be contaminated or suspiciously contaminated by BVDV(account for 16.25%) respectively, and anather 3 contaminated by OV (3.8%) . On these results basis, the authors suggest that this preparation is of both high specificity and sensitiveity and of high practicability and reliability.

用猪瘟病毒(HCV)、猪细小病毒(PPV)、牛病毒性腹泻/粘膜病病毒(BVD/MDV)、伪狂犬病病毒(PrV)、兰舌病病毒(BtV)、羊口疮病毒(OV)等六种病毒,多次同时或先后强化免疫猪,制取六价高免血清;用狂犬病病毒(RV)免疫兔,制取RV单价高免血清。分别用异硫氰酸荧光素(FITC)标记制成荧光抗体(FA)。并按二者最佳染色价配制成七价(多价)FA。由于各病毒在其宿主细胞中复制部位和与宿主细胞相互关系各不相同,在荧光显微镜下,多价FA对其相应病毒的细胞培养物,能显示出各不相同的荧光特点,而得以检测、鉴定。用相应的多、单价高免血清作抑制染色试验,结果各病毒荧光可被一一抑制,表明了多价FA具有高度的特异性。对各病毒进行FA细胞培养物半数感染量(FA-TCID50)与常规TCID50或LD50测定比较试验,结果前者均高于后者,表明了这种荧光抗体制剂同时具有高度的敏感性。应用多价FA,对非禽源细胞苗生产中外源病毒的污染进行了中间监测,选用了与强毒荧光无区别的7种感染的细胞作阳性参照,保证了生产单位在使用中的安全,对A、B二厂生产猪瘟苗有干扰的细胞,进行抽样监测80批,结果13批污染BVDV(16.25%),1?

Aim: To select suitable virus strains and celllines for experiment of inactivation of Poliovirus in water.Metheds: Comparing the results of cytopathic effects (CPE), 50% tissue culture infective doses (TCID50 ), plaqueforming units (PFU) and dot immunobinding assays(DIBA) after 5 virus strains were separately propagated for3~5 days in 5 cell lines. Results: Determination of CPEand TCID 50 showed that ① the virulence of 5 strains to thesame cell line ranged from the highest to the lowest asHenan,...

Aim: To select suitable virus strains and celllines for experiment of inactivation of Poliovirus in water.Metheds: Comparing the results of cytopathic effects (CPE), 50% tissue culture infective doses (TCID50 ), plaqueforming units (PFU) and dot immunobinding assays(DIBA) after 5 virus strains were separately propagated for3~5 days in 5 cell lines. Results: Determination of CPEand TCID 50 showed that ① the virulence of 5 strains to thesame cell line ranged from the highest to the lowest asHenan, Hebei >Brunhide, Mahony> Sabin; ② Thesensitivities of 5 infected cell lines to the same strain were thefollowing range: Hep-2>BGM>Hela, Vero>RD.Detection of PFU and DIBA indicated that Henan strain was inspecied more easily than Brunhide strain after both strainswere propagated in Hep-2 cells. Conclusion: PV 1 Henanstrain and Hep-2 cell line are appropriate to the edperimentof inactivation of PV in water.

目的:根据5种细胞系对脊髓灰质炎病毒1型(PV1)5个毒株的敏感性筛选适用于病毒消毒实验的病毒株和细胞系.方法:比较各毒株在不同细胞系中增殖3d~7d后的细胞病变作用(CPE)、半数组织培养感染里(TCⅡ)50)、蚀斑计数(PFU)及免疫印迹(DIBA)试验结果.结果:CPE与TCID)检测表明,同一细胞系Hep2和Hela中病毒感染细胞作用强弱为Henan,Hebei>Brunhide,Mahony>Sabin株;不同细胞系对同一病毒株的敏感性为Hep-2>BGM>Hela,Vero>RD细胞.DBA检测提示,Henan株较Brun-hide更易检出.结论:Henan株及Hep-2细胞可作为PV消毒实验所用的病毒和细胞.

 
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