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The formation of a violet or pink colored complex of Co~(++) with p-nitro-phenylhydrazone of diacetylmonoxime in the presence of ammonium hydroxidewas utilized in the colorimetric determination of microgram quantities ofcobalt. Ammonium acetate, together with ethyl alcohol, was used to destroythe violet color developed by the interaction between ammonium hydroxide andthe reagent. Pulfrich photometer, with S53 filter and 1 cm cell, was used. Beer's lawwas obeyed over the range 0--5 γ cobalt per ml solution.... The formation of a violet or pink colored complex of Co~(++) with p-nitro-phenylhydrazone of diacetylmonoxime in the presence of ammonium hydroxidewas utilized in the colorimetric determination of microgram quantities ofcobalt. Ammonium acetate, together with ethyl alcohol, was used to destroythe violet color developed by the interaction between ammonium hydroxide andthe reagent. Pulfrich photometer, with S53 filter and 1 cm cell, was used. Beer's lawwas obeyed over the range 0--5 γ cobalt per ml solution. The method devised is simple, rapid, and reproducible. 在氨水、乙醇、醋酸铵存在下,以丁二酮单肟对硝基苯腙作钴的比色,在0—5γ钴/ml之间符合Beer定律。此法操作简便、迅速而再现性高。 Cepheid variables CY Aqr and BE Mon were observed at the Purple Mountain Observatory with a photoelectric photometer during December 1959 and the first two months of 1960.The observations of CY Aqr (Table 1) made on four nights provide sufficient data for plotting five complete light curves (Fig.1) of this variable.The phase of these observations has been computed with Lange and Nekrasova's formula.We noticed that the maxima of the plotted curves do not fall on the zero phase,but show a shift of about... Cepheid variables CY Aqr and BE Mon were observed at the Purple Mountain Observatory with a photoelectric photometer during December 1959 and the first two months of 1960.The observations of CY Aqr (Table 1) made on four nights provide sufficient data for plotting five complete light curves (Fig.1) of this variable.The phase of these observations has been computed with Lange and Nekrasova's formula.We noticed that the maxima of the plotted curves do not fall on the zero phase,but show a shift of about 0.05φin phase toward the left.In other words, the maximum light occurs four or five minutes ahead of the time predicted by this formula. 用紫金山天文台的60厘米反光望远镜和1P21光电倍增管所构成的光电光度计,我们在1959年底和1960年初,对 CY Aqr 及 BE Mon 进行了测光观测.得到 CY Aqr 的五条完整的光变曲线.我们采用了从1934年到现在的29个观测所得的极大历元来和公式计算的相比较.若不承认历元有跃迁的可能,那末任何线性公式,都难以满足这些观测数据的要求.我们按周期随时缩短的假设,得出下列比较满意的公式:Max⊙=I.D.2427658.4079十0~d.061038576E—(0~d.742×10~(-12))E~2.用前后将近两个月观测 BE Mon 所获得的数据,我们得到了这颗造父变星的光变曲线.变星总表引用所定0~d.421的周期是不正确的.根据我们观测所得到的周期是2~d.704.按这周期来整理于1935年所完成的目视观测,我们也得出了一个很象样的光变曲线. Paris' method for the determination of anthraquinone content of plant drug has been studied. Through the analysis of several drugs, including Rheum palmatum L., Cassia tora L., and Polygonum multiflorum Thunb., more favorable analytical conditions were established; the free and combined anthtaqiiinone contents of a plant drug could thus be determined as follows: Free anthraquinones: 0.1—l.0g finely powdered sample is accurately weighed and extracted with chloroform in a Soxhlet apparatus. The chloroform extract... Paris' method for the determination of anthraquinone content of plant drug has been studied. Through the analysis of several drugs, including Rheum palmatum L., Cassia tora L., and Polygonum multiflorum Thunb., more favorable analytical conditions were established; the free and combined anthtaqiiinone contents of a plant drug could thus be determined as follows: Free anthraquinones: 0.1—l.0g finely powdered sample is accurately weighed and extracted with chloroform in a Soxhlet apparatus. The chloroform extract is shaken with successive portions of 5% sodium hydroxide-2% ammonia mixture in a separatory funnel until the alkali solution is colourless. The alkali extracts are combined and diluted to a certain volume. If the solution becomes turbid, it is filtered through a sintered glass filter and the filtrate collected for colorimetric determination in a photometer with a 490 mμ filter. The result is calculated from a calibration curve obtained with 1,8-dihydroxyanthraquinone. The extraction with the mixed alkali solution and the colorimetric measurements must be done in a shaded room to prevent the decomposition of the coloured solution by light. Combined anthraquinones: 0.05—0.1g finely powdered sample is accurately weighed and placed in a 100 ml conical flask, 30 ml of 5 N sulphuric acid are added, and the mixture is refluxed for two hours to hydrolyse the combined anthraquinones. The flask is cooled, then refluxed with 30 ml chloroform for one hour. The latter is removed with a dropper, replaced by a fresh portion of 20 ml chloroform and refluxed for 20 minutes. This extraction process is repeated until anthraquinones are exhausted. The chloroform extracts are combined, washed with small portions of distilled water, extracted with the mixed alkali solution as above, and determined colorimetrically. This gives the total anthraquinone content, from which is subtracted the amount of free anthraquinones in order to get the percentage of combined anthraquinones. 本文在Paris等人所报导方法的基础上,研究了蒽醌显色后的稳定性情况,以及用酸水解结合蒽醌与提取的条件,进而分析了几种植物药中游离蒽醌与结合蒽醌的含量,认为方法尚可应用.游离蒽醌系将生药样品在Soxhlet提取器内用氯仿提取,提取液用5%NaOH-2%NH_40H提取后比色,以1,8-二羟蒽醌为标准;结合蒽醌则先用5N硫酸水解,回流2小时,再加氯仿回流提取数次,至提尽为止,合并氯仿液,同上以碱液提取后比色测定.
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