|
The test pigs were done a surgery that equipped with chonic catheters of carotid artery,portal vein and ileal vein And the technique was used by infusion of p-aminohipuric acid (PAH) into theileal vein.The values for blood hematocrit,portal vein plasma flow rate (PVPF) or portal vein blood flow rate (PVBF) in pigs fed a couple of diets were measured.The results were showed,as the collection times of blood sampling were changed,whether preprandial or postprandial period of 8... The test pigs were done a surgery that equipped with chonic catheters of carotid artery,portal vein and ileal vein And the technique was used by infusion of p-aminohipuric acid (PAH) into theileal vein.The values for blood hematocrit,portal vein plasma flow rate (PVPF) or portal vein blood flow rate (PVBF) in pigs fed a couple of diets were measured.The results were showed,as the collection times of blood sampling were changed,whether preprandial or postprandial period of 8 hours after intake of different dites,the content of portal or arterial blood hematocrit had no effect in pigs fed a couple of diets(P>005). And the values of portal or arterial blood hematocrit were 3225%(Diet A) and 3260%(Diet B) or 3100%(Diet A) and 3340%(Diet B),respectively,in preprandial period.And in postprandial period of 8 hours the mean values of portal or arterial blood hematocrit were 2869 % (Diet A) and 2900 %(Diet B) or 2897%(Diet A) and 2903% (Diet B),respectively.The rate of PVPF and PVBFin pigs had also no effect as the diets were changed (P>005).In preprandial period,the rate of PVPFor PVBF were 3134 (DietA) and 2874mL/min·kg-1BW (Diet B) or 4799 (Diet A) and4430 mL/min·kg-1BW (Diet B),respectively.And in postprandial period of 8 hours the mean rate of PVPF or PVBF were 2155 (Diet A) and 2922 mL/min·kg-1BW (Diet B) or 3006 (Diet A) and 4162 mL/min·kg-1BW (Diet B),respectively 通过对试验猪实施颈动脉、门静脉和回肠肠系膜静脉血管插管手术,并结合回肠肠系膜静脉灌注对氨基马尿酸(PAH)技术,探讨了两种日粮对猪红细胞压容积含量以及门静脉血浆流率(PVPF)和血液流率(PVBF)的影响。结果表明,随着血样采集时间的变化,不论是采食前还是食后8h内(每隔1h收集一次血样),两种日粮对猪门静脉或颈动脉血液红细胞压容积含量的变化都没有影响(P>0.05)。采食前,猪门静脉血液红细胞压容积含量为32.25%(日粮A)和32.60%(日粮B),而食后8h内其平均含量为28.69%(日粮A)和29.00%(日粮B);猪颈动脉血液红细胞压容积含量在采食前为31.00%(日粮A)和33.40%(日粮B),在食后8h为28.97%(日粮A)和29.03%(日粮B)。猪门静脉血浆流率和血液流率也不受日粮变化的影响(P>0.05)。采食前,猪门静脉血浆流率为31.34(日粮A)和28.74mL/min·kg-1BW(日粮B),血液流率为47.99(日粮A)和44.30mL/min·kg-1BW(日粮B);而食后8h内门静脉血浆平均流率为21.55(日粮A)和29.22mL/min·kg-1BW(日粮B),血液平... 通过对试验猪实施颈动脉、门静脉和回肠肠系膜静脉血管插管手术,并结合回肠肠系膜静脉灌注对氨基马尿酸(PAH)技术,探讨了两种日粮对猪红细胞压容积含量以及门静脉血浆流率(PVPF)和血液流率(PVBF)的影响。结果表明,随着血样采集时间的变化,不论是采食前还是食后8h内(每隔1h收集一次血样),两种日粮对猪门静脉或颈动脉血液红细胞压容积含量的变化都没有影响(P>0.05)。采食前,猪门静脉血液红细胞压容积含量为32.25%(日粮A)和32.60%(日粮B),而食后8h内其平均含量为28.69%(日粮A)和29.00%(日粮B);猪颈动脉血液红细胞压容积含量在采食前为31.00%(日粮A)和33.40%(日粮B),在食后8h为28.97%(日粮A)和29.03%(日粮B)。猪门静脉血浆流率和血液流率也不受日粮变化的影响(P>0.05)。采食前,猪门静脉血浆流率为31.34(日粮A)和28.74mL/min·kg-1BW(日粮B),血液流率为47.99(日粮A)和44.30mL/min·kg-1BW(日粮B);而食后8h内门静脉血浆平均流率为21.55(日粮A)和29.22mL/min·kg-1BW(日粮B),血液平均流率则为30.06(日粮A)和41.62mL/min·kg-1BW(日粮B)。 The study was conducted to determine the effects of galacto-mannan-oligosaccharides(GMOS)on portal vein plasma flow(PVPF)rate,portal vein blood flow(PVBF)rate,net appearance(% of intake)of amino acids and glucose in portal blood,and O2 consumption by the portal vein-drained organs in growing pigs.After 14 days adapting periods in mental metabolite cage,ten pigs(initial body weight of 15 kg)were fitted with chronic catheters in carotid artery,portal vein,and ileal mesenteric vein,simultaneously.Pigs... The study was conducted to determine the effects of galacto-mannan-oligosaccharides(GMOS)on portal vein plasma flow(PVPF)rate,portal vein blood flow(PVBF)rate,net appearance(% of intake)of amino acids and glucose in portal blood,and O2 consumption by the portal vein-drained organs in growing pigs.After 14 days adapting periods in mental metabolite cage,ten pigs(initial body weight of 15 kg)were fitted with chronic catheters in carotid artery,portal vein,and ileal mesenteric vein,simultaneously.Pigs were randomly allocated to two groups and fed corn-soybean meal-based diets supplemented with 0 or 0.20% GMOS(n=5 per group).At the end of 15 days feeding trial,blood and plasma flows were measured with infusion of p-aminohipuric acid(PAH)into the ileal vein.At 8 h after feeding,dietary supplementation with GMOS decreased(P<0.05)PVPF and PVBF in portal vein,increased(P<0.05)net absorption of amino acids and glucose into portal vein,and decreased(P<0.05)O2 consumption by the portal vein-drained organs,when compared with the control pigs.Our results suggest that GMOS reduces the oxidation of amino acids and glucose by the small-intestinal mucosa in growing pigs,thereby enhancing their availabilities for utilization by extraintestinal tissues and cells. 通过对10头15kg体重试验猪实施门静脉血管插管等外科手术,并结合回肠肠系膜静脉灌注对氨基马尿酸(PAH)技术,探讨半乳甘露寡糖对生长猪门静脉血浆流率(PVPF)和血液流率(PVBF)、氨基酸和葡萄糖的净吸收量及耗氧量的影响。10头杜长大阉公猪随机分为2个处理,每个处理5头,单笼饲养于可调节不锈钢代谢笼内,经过14d适应期后,在门静脉、肠系膜静脉、颈动脉安装插管,开始15d正式试验。对照组饲喂玉米-豆粕型基础饲粮,试验组饲喂基础饲粮+0.20%半乳甘露寡糖。结果表明,经过15d饲喂期后,与对照组相比,饲粮添加半乳甘露寡糖可显著降低试猪食后8h内门静脉的平均血浆流率和血液流率,显著提高食后8h内门静脉对氨基酸和葡萄糖的净吸收量,显著降低食后8h内门静脉的耗氧量,也就是说半乳甘露寡糖可通过减少生长猪小肠黏膜对氨基酸和葡萄糖的氧化而增加肠外组织对其吸收。
|