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The livers from 5 week 14~32 human fetuses were studied. The results are summarized as follows:1. Two kinds of liver cells revealed by EM: the "light" and "dark" cells. The light ones are larger in size, more numerous in number, containing larger mitochondria and obvious RER and SER, but less free ribosomes than the dark ones. Both of them may contain hemosiderin granules and the configuration of mitochondria enclosed by circular or semicircular RER cisternae.2. Bile canaliculus: Beside canaliculi between... The livers from 5 week 14~32 human fetuses were studied. The results are summarized as follows:1. Two kinds of liver cells revealed by EM: the "light" and "dark" cells. The light ones are larger in size, more numerous in number, containing larger mitochondria and obvious RER and SER, but less free ribosomes than the dark ones. Both of them may contain hemosiderin granules and the configuration of mitochondria enclosed by circular or semicircular RER cisternae.2. Bile canaliculus: Beside canaliculi between two adjacent liver cells, canaliculi situated between 3 or 4 liver cells are not infrequently revealed. That is due to the fetal liver cells are arranged in groups rather than in cords. The stereoscopic configurations of bile canaliculus and the tight junction surrounding it are demonstrated more clearly in freeze-fracture micrograph than that of the TEM. In addition, one intracellular bile canaliculus is found in the freeze-fracture preperation.3. The blood sinusoid and hemopoietic focus: In the 4-week fetus, the endothelium of the sinusoid can hardly be recognized While its lumen is quite large. Many erythrocytes accompanied by their immature components can be seen among it. As to the 32-week fetal liver, the endothelium and Kupffer cell may be easily recognized but the lumen of the sinusoid dimenishes enormously. The hemopoietic foci are localized extra-sinusoidially and in close contact with liver cells. Their volume exceeds one half of the fetal liver lobule before the age of 28 weeks, then it dimenishes gradually. In the space of Disse a fat storage cell is revealed.4. The portal canal : The mensenchyma and immature erythrocytes in the portal canal dimenishes with the increase of the fetal age. The portal canal is surrounded by the pigmented limiting plate cells of the liver lobules. The interlobular bile duct is differentiated from the limiting plate cells. Its epithelium is composed of both light and dark cells. The former is more numerous and contains many large mitochondria in its apical portion, while the latter shows higher electron density. Microvilli are located on the surfaces of both kinds of cells and finger like processes in the enlarged intercellular spaces are revealed. 本研究采用5例正常人14~32周水囊引产胎儿肝脏,进行了光镜、超薄切片和冷冻复型电镜的对比观察,发现并证实了: 1.肝细胞有明、暗之分, 2.肝细胞内有胆小管, 3.小叶间胆管由肝小叶的界板细胞形成,而构成管壁的上皮细胞也有明、暗之分。最后,根据观察所见并结合已有文献进行了分析讨论。 Liver specimens of 12 newborns are studied. In the newborn, adjucent lobules are not outlined precisely. The liver plates sepanated by hepatic sinusoids arpear as masses of cords in the section Hepatecytes are polyhedral cells that contain thin eosinephil granules,The clear and dark stained cells are obseived in 3 cases. There are scatters d hematopoietic focuses between the liver cord and hepatic sinusoid, or in hepatic sinusoids. There are one or two layers of periportal limiting plate cells around the portal... Liver specimens of 12 newborns are studied. In the newborn, adjucent lobules are not outlined precisely. The liver plates sepanated by hepatic sinusoids arpear as masses of cords in the section Hepatecytes are polyhedral cells that contain thin eosinephil granules,The clear and dark stained cells are obseived in 3 cases. There are scatters d hematopoietic focuses between the liver cord and hepatic sinusoid, or in hepatic sinusoids. There are one or two layers of periportal limiting plate cells around the portal area. The interlobular bile ducts derived from the peripotal limiting plate are observed. 本文收集新生儿肝脏12例,用Bouin氏液固定,HE染色。镜下见肝小叶结构明显,相邻肝小叶分界不清,肝板在切面上呈团块状或条索状,与肝血窦交错排列,肝细胞呈多边形,胞质中有细小的嗜酸性颗粒,有3例围成团块状的肝细胞着色深浅不等。肝小叶内有散在的造血灶,门管周围有1~2层门管周围界板细胞。 Histochemical observasion and comparason have been made of biliary passage and gall bladder of normal dogs and gall stone dogs by HE and Alcian bluc-PAS and HTD-AB method.Results show that epithelial secretory functions of liver cells biliary passage,interlobular bile duet and mucosa of gall stone dogs become greater.The gall stone is formed by neutral and acid sulfoglycoproteion,which have been secreted from liver cells,biliary passage interlobular bile duct musosa and glands of gall stone... Histochemical observasion and comparason have been made of biliary passage and gall bladder of normal dogs and gall stone dogs by HE and Alcian bluc-PAS and HTD-AB method.Results show that epithelial secretory functions of liver cells biliary passage,interlobular bile duet and mucosa of gall stone dogs become greater.The gall stone is formed by neutral and acid sulfoglycoproteion,which have been secreted from liver cells,biliary passage interlobular bile duct musosa and glands of gall stone dogs. 狗肝胆道系统组织化学及狗宝分泌形成初步研究¥实验动物中心(226001)邵义祥,朱顺星,毕书增,高长泽关键词狗宝;分泌与形成;肝胆道系统;狗狗宝是珍贵药材,是狗胆囊、膀胱等内脏器官的结石[1],自然形成的结石极少,在狗体内培植形成狗宝已有报道[2]....
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