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insecticide metabolism
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  相似匹配句对
     METABOLISM OF PYRETHROID INSECTICIDE CYPERMETHRIN IN GOLDEN ORFE
     拟除虫菊酯类农药氯氰菊酯在鱼(Golden Orfe)体中的代谢
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     Growth and metabolism of D.
     结果表明 :石斛的生长与代谢随温度由低到高 ,表现出弱 -强 -弱的变化规律 ;
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     Conclusion The lung is involved in fenvalerate metabolism which suggested the importance of pulmonal toxicity induced by the insecticide.
     结论 肺脏优势参与了氰戊菊酯的代谢过程 ,应重视此类农药的肺脏毒性。
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     The Synthesis of Insecticide Acetadpdd
     杀虫剂啶虫脒的合成
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  insecticide metabolism
Finally, most insecticide-resistance studies in which elevated GSTs have been detected are subsequently analysed by insecticide metabolism studies.
      


Objective To perform prokaryotic expression,purification and enzymatic activity assay of NYD-Ch,a chymotrypsin associated with insecticide resistance from Culex pipiens pallens.Methods By PCR and gene reconstructed technology,the recombinant plasmid pET-32a(+)/NYD-Ch was constructed and expressed.Then the expressed protein was purified through Ni~(+) column chromatography.Enzymatic activity was assayed by different substrates and pH.Results The recombinant plasmid was transformed in E.coli BL21 and expressed...

Objective To perform prokaryotic expression,purification and enzymatic activity assay of NYD-Ch,a chymotrypsin associated with insecticide resistance from Culex pipiens pallens.Methods By PCR and gene reconstructed technology,the recombinant plasmid pET-32a(+)/NYD-Ch was constructed and expressed.Then the expressed protein was purified through Ni~(+) column chromatography.Enzymatic activity was assayed by different substrates and pH.Results The recombinant plasmid was transformed in E.coli BL21 and expressed NYD-Ch protein.A tape of protein about 48.5 ku could be viewed according to SDS-PAGE,which was coincident with the designed protein. The result was confirmed by Western blot.Enzymatic activity assay demonstrated that the expressed product had the common features of chymotrypsin: NYD-Ch was able to hydrolyze the synthetic substrate S(Ala)_(2)ProPhe-pNA and it was strongly inhibited by PMSF,SBTI and TPCK.The activity of NYD-Ch had strongly alkaline pH optima,range from pH 8.0 to 11.0.Optimal activity was observed at pH 10.0.Conclusion Successgul prokaryotic expression,purification and enzymatic activity assay of NYD-Ch should greatly facilitate attempts to learn its function of insecticide metabolism.

目的表达、纯化淡色库蚊溴氰菊酯抗性相关糜蛋白酶(NYD-Ch)基因并进行酶活性分析。方法应用PCR和基因重组技术,构建原核表达载体,表达目的蛋白;用酶促底物法测定表达蛋白NYD-Ch的酶学活性和最适pH值。结果成功构建重组表达载体pET32a(+)/NYD-Ch,并转化到感受态菌E.coliBL21(DE3)中。SDS-PAGE和Western blot显示,目的蛋白分子质量与理论值相符。NYD-Ch最大活力通过S(Ala)2ProPhe-pNA获得。NYD-Ch活力随pH的增高而增大,在pH 8.01~11.0的范围内较高,pH 10.0时达最高。抑制剂PMSF、SBTI、TPCK对NYD-Ch酶活力均有明显的抑制作用。结论成功表达了NYD-Ch蛋白,并分析了其酶学活性。本结果为深入研究NYD-Ch与杀虫剂抗性关系奠定基础。

 
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