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capsule stain
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  “capsule stain”译为未确定词的双语例句
     After the animal episoded,we dissected them and the mouse's pyoperitoneum. The thickness of capsule can be observed by Hiss capsule stain,microscopic observation,and be surveyed by microscopic surveying instrument.
     待实验动物发病后解剖动物,分别取家兔肾组织印片及小鼠腹腔液涂片进行Hiss荚膜染色镜检,用显微测微计测量荚膜层的厚度,各菌株随机计数40个酵母细胞的荚膜层的厚度值,取各菌株荚膜层厚度的均值进行比较。
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  相似匹配句对
     . and Ranjianduoji capsule.
     1、本草考证与文献考查
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     Fabric Stain Removal
     织物上油污斑渍的去除
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     Method Immunohistochemical stain.
     方法用免疫组化方法研究。
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     “BLACKOUT” AND DATA CAPSULE
     “黑障"与数据舱回收装置
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     The morphology of the virus was similar to round and hexagonal without capsule, diameter 30 nm by negative stain under electron microscope.
     电镜观察负染标本病毒形态近似圆形和六角形,无胞膜,直径约30nm。
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  capsule stain
This mutant excluded the capsule stain India ink, and this exclusion was lost in the presence of the enzyme hyaluronidase.
      


Objective: To observe the thickness of the capsule of the standard strain(CCCMC1a、ATCC14053)and the clinical isolation strain (C1-1、C1-2、C1-3、C1-4)of candida albicans in different condition.Methods:The strains smeared on sabourand ager medium for 36h are diluted into the bacterial solution of 1×109/ml by physiological saline.1ml of this solution is injected in a rabbit's verge vein of ear,and 0.5ml in a mouse's abdominal cavity.After the animal episoded,we dissected them and the mouse's pyoperitoneum.The thickness...

Objective: To observe the thickness of the capsule of the standard strain(CCCMC1a、ATCC14053)and the clinical isolation strain (C1-1、C1-2、C1-3、C1-4)of candida albicans in different condition.Methods:The strains smeared on sabourand ager medium for 36h are diluted into the bacterial solution of 1×109/ml by physiological saline.1ml of this solution is injected in a rabbit's verge vein of ear,and 0.5ml in a mouse's abdominal cavity.After the animal episoded,we dissected them and the mouse's pyoperitoneum.The thickness of capsule can be observed by Hiss capsule stain,microscopic observation,and be surveyed by microscopic surveying instrument.The thickness of a speciman's average capsule defined by the average thickness of 40 sacccharomycetic cell is compared with the others.Results:(1)The standard strain and the clinical isolation strain can be capsule both in infected mouse and rabbit.The thickness of the capsule is changeable because of different strains and conditions(the average:0.25~0.58μm,P<0.01 or P<0.05).(2)Capsule swelling experiment shows that there are intersect positive reaction between the strain specimen.Conclusion: the thickness of the capsule of candida albicans is changeable because of different strains and conditions.Capsule swelling experiment shows that there are intersect positive reaction between the strain specimen.

目的:观察白假丝酵母菌标准菌株(CCCMC1a、ATCC 14053)与临床分离菌株(C1-1、C1-2、C1-3、C1-4)于不同条件下荚膜层的厚度变化。方法:(1)将实验菌种沙保氏琼脂36小时培养物用生理盐水配成约含1×109个/ml的菌悬液,家兔耳缘静脉注射,1ml/只,同时用BALB/c小鼠腹腔注射,0.5ml/只;待实验动物发病后解剖动物,分别取家兔肾组织印片及小鼠腹腔液涂片进行Hiss荚膜染色镜检,用显微测微计测量荚膜层的厚度,各菌株随机计数40个酵母细胞的荚膜层的厚度值,取各菌株荚膜层厚度的均值进行比较。(2)实验菌株沙保氏液体培养物与同一菌株的抗血清作荚膜肿胀实验及血清交叉实验,Hiss荚膜染色镜检。荚膜层厚度的测量与计算方法同前。结果:(1)标准菌株与临床分离菌株在家兔及小白鼠体内均可形成荚膜,荚膜层的厚度可因不同菌种(株)和不同环境而有差异(均值在0.25~0.58μm不等;P<0.01或<0.05),边界明显。(2)用全菌抗血清作荚膜肿胀实验,可见菌细胞的荚膜明显加宽,且菌株之间可出现交叉阳性。结论:白假丝酵母菌的荚膜厚度可因不同菌种(株)和不同环境而有差异;荚膜肿胀实验显示各菌株除与相...

目的:观察白假丝酵母菌标准菌株(CCCMC1a、ATCC 14053)与临床分离菌株(C1-1、C1-2、C1-3、C1-4)于不同条件下荚膜层的厚度变化。方法:(1)将实验菌种沙保氏琼脂36小时培养物用生理盐水配成约含1×109个/ml的菌悬液,家兔耳缘静脉注射,1ml/只,同时用BALB/c小鼠腹腔注射,0.5ml/只;待实验动物发病后解剖动物,分别取家兔肾组织印片及小鼠腹腔液涂片进行Hiss荚膜染色镜检,用显微测微计测量荚膜层的厚度,各菌株随机计数40个酵母细胞的荚膜层的厚度值,取各菌株荚膜层厚度的均值进行比较。(2)实验菌株沙保氏液体培养物与同一菌株的抗血清作荚膜肿胀实验及血清交叉实验,Hiss荚膜染色镜检。荚膜层厚度的测量与计算方法同前。结果:(1)标准菌株与临床分离菌株在家兔及小白鼠体内均可形成荚膜,荚膜层的厚度可因不同菌种(株)和不同环境而有差异(均值在0.25~0.58μm不等;P<0.01或<0.05),边界明显。(2)用全菌抗血清作荚膜肿胀实验,可见菌细胞的荚膜明显加宽,且菌株之间可出现交叉阳性。结论:白假丝酵母菌的荚膜厚度可因不同菌种(株)和不同环境而有差异;荚膜肿胀实验显示各菌株除与相应抗血清作用出现荚膜肿胀实验阳性外菌株之间还可出现血清交叉阳性。

To observe and confirm the capsule structure of four clinical strains of Candida albican(C1-1、C1-2、 C1-3 、C1-4).(1)Four clinical vagina specimens of female patient with venereal disease were smeared on slides and cultured on sabouraud agar medium by streak-plate method.Then the isolated colony was incubated in slant and liquid sabouraud medium.The yeast like cells were identified by Gram stain and Hiss capsule stain, microscopic observation, and by budding development, chlamydospore formation, sugar fermentation.(2)...

To observe and confirm the capsule structure of four clinical strains of Candida albican(C1-1、C1-2、 C1-3 、C1-4).(1)Four clinical vagina specimens of female patient with venereal disease were smeared on slides and cultured on sabouraud agar medium by streak-plate method.Then the isolated colony was incubated in slant and liquid sabouraud medium.The yeast like cells were identified by Gram stain and Hiss capsule stain, microscopic observation, and by budding development, chlamydospore formation, sugar fermentation.(2) Capsular structure was observed by animal test with rabits and BALB/C mice.In addition, the quellung reaction was tested and the electron microscopy of the organism from infected Rabits and BALB/C mice was studied.(1) The four clinical strains(C1-1、C1-2、 C1-3 、C1-4) are Gram positive and assumes prayer beads shaped.It can produce germtubes, pseudohyphae and chlamydospore, and can ferment glucose and maltose with acid and gas production, can ferment sucrose with acid but no gas.But it cannot ferment lactose.(2) Capsular structure can be seen in the yeast-like cells on the smear from infected mouse and rabbit tissues.Though the formation of the capsule is apparently related to culture environment and culture time(P<0.01/<0.05), capsular structure is very clear and the quellung reaction is positive.The four clinical strains of Candida albicans can be identified with a capsule-like structure.

取临床标本(性病门诊病人阴道分泌物)直接涂片染色镜检及用沙保氏琼脂平板分离培养、作涂片革兰染色镜检、出芽试验、厚膜孢子形成试验、糖发酵试验等鉴定菌种;小白鼠与家兔试验、荚膜肿胀试验、透射电镜等观察荚膜,观察白假丝酵母菌临床分离菌株(C1-1、C1-2、C1-3、C1-4)的荚膜结构并探讨其形成条件。结果显示:(1)C1-1、C1-2、C1-3、C1-4四株临床分离菌株均为革兰阳性、念珠状菌;能形成芽管、假菌丝、厚膜孢子;能发酵葡萄糖和麦芽糖产酸又产气;发酵蔗糖产酸不产气;不发酵乳糖。(2)在感染小白鼠及家兔体内均可形成荚膜,荚膜层的厚度可因不同环境而有显著差异(P<0·01或<0·05),边界明显;荚膜肿胀试验阳性。4株临床分离菌株均可被鉴定为具有荚膜的白假丝酵母菌。

 
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