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potency of gene
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  “potency of gene”译为未确定词的双语例句
     A REPORT ON A IMMUNIZING POTENCY OF GENE HEPATITIS B VACCINE.
     基因乙肝疫苗免疫效果随访2年报告
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     The contraction potency was voltage-dependent.
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     Gene Transfer in Higher Plants and Its Potency in Crop Improvement
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  potency of gene
Objective: To study the vaccine potency of gene-modified tumor cells.
      
Therefore, synthetic molecules capable of recruiting such complexes to the promoter could synergistically enhance the potency of gene induction.
      
Several approaches for increasing the potency of gene-based vaccines were presented.
      


In order to study the vaccine potency of gene modified tumor cells, IL 12 express vector was constructed by using retrovirus. The vector was transfected into EL 4 thymic lymphoma cells and the effect of transfectant on anti tumor immunity was investigated. The tumorigenicity of EL 4/IL 12 transfectant in syngeneic C57BL/6 mice was decreased significantly after implanted with EL 4/IL 12 transfectant compared with EL 4/Wt or EL 4/Neo groups (P<0.001). The systemic protective immunity was induced...

In order to study the vaccine potency of gene modified tumor cells, IL 12 express vector was constructed by using retrovirus. The vector was transfected into EL 4 thymic lymphoma cells and the effect of transfectant on anti tumor immunity was investigated. The tumorigenicity of EL 4/IL 12 transfectant in syngeneic C57BL/6 mice was decreased significantly after implanted with EL 4/IL 12 transfectant compared with EL 4/Wt or EL 4/Neo groups (P<0.001). The systemic protective immunity was induced against the challenge with EL 4/Wt (in 8/10 mice) after the rejection of EL 4/IL 12 in vivo experiment, a stronger CTL activity against EL 4/Wt cells and a weak killer activity against syngeneic Lewis lung carcinoma (LLC) cells were obtained in 51 Cr release assay. In vivo depletion analysis suggested that the decreased tumorigenicity mainly depended on CD4 +, CD8 + and NK cells. Therapeutic vaccines with EL 4/IL 12 cells could retard the growth of established EL 4/Wt tumors significantly compared with those of EL 4/Neo (P<0.005). These studies suggested that immunotherapy and gene therapy using IL 12 is effective in hematopoietic malignancy and IL 12 has prospects of application in human cancer treatment in the near future.

应用逆转录病毒构建了IL 12表达载体 ,以研究IL 12基因修饰的肿瘤细胞的肿瘤疫苗作用 ,将其转染EL 4胸腺瘤细胞并研究了该基因导入细胞的抗肿瘤免疫效果。当接种了EL 4 /IL 12转染细胞后 ,在C5 7BL/ 6同系鼠中其基因导入细胞的致瘤性比EL 4 /Wt和EL 4 /Neo组明显减少 (P <0 .0 1) ,在EL 4 /IL 12被排斥后 ,体内试验中实验动物诱发了抗EL 4 /Wt的系统性、保护性免疫 ,51Cr释放测定中 ,获得一个较强的抗EL 4 /Wt和一个较弱的抗同系Lewis肿瘤细胞的CTL活性 ,体内淋巴细胞消除分析的结果提示减少的致瘤性主要依赖于CD4 +,CD8+和NK细胞。这一研究结果提示应用IL 12进行血液肿瘤的治疗是有效的 ,在未来人类癌症的治疗中IL 12亦可有一定的应用前景

Objectire To improve the vaccine potency of gene-modified tumor cells Methods Using recombinant adenoviruses,we expressed the B7.1 gene in murine breast tumor cell line EMF 6 and a subline previously transfected with retrovirus vector XdF containing IL-2-TNFα fusion gene. Rescults Immunization/challenge experiments demonstrated that IL-2-TNFα/B7.1 co-modified tumor cells possessed a lower tumorigenicity in vivo and an improved tumor-specific vaccine potency when compared to single...

Objectire To improve the vaccine potency of gene-modified tumor cells Methods Using recombinant adenoviruses,we expressed the B7.1 gene in murine breast tumor cell line EMF 6 and a subline previously transfected with retrovirus vector XdF containing IL-2-TNFα fusion gene. Rescults Immunization/challenge experiments demonstrated that IL-2-TNFα/B7.1 co-modified tumor cells possessed a lower tumorigenicity in vivo and an improved tumor-specific vaccine potency when compared to single gene transfectant(P<0.05). Three weeks after immunization with a variety of tumor cells,mixed lymphocyte culture assay and 51 Cr-release assay were performed to test the cellular immunity activities. The results indicated that IL-2-TNFα and B7.1 together induced a more potent antitumor immune response compared to either molecule alone (25% and 20% higher than IL-2-TNFα and B7.1 respectively). Conclusion Our results suggest that an improved tumor cell vaccine efficacy can be acquired by the concerted action of IL-2-TNFα and B7.1.

目的 改进基因修饰的肿瘤细胞疫苗的效力。方法 在小鼠乳腺癌细胞系EMF6 和一个已被含有IL - 2和TNFα融合基因的逆转录病毒载体XdF转染的EMF6 亚系 (T2 -EMF6)中表达B7 1基因。结果 免疫 /侵袭实验表明 ,与单基因转导的肿瘤细胞相比 ,IL - 2-TNFα/B7 1共同修饰的肿瘤细胞具有较低的致瘤性和较优的疫苗效力 (P <0 0 5)。在各种肿瘤细胞的免疫接种后三周 ,进行混合淋巴细胞培养实验和51 Cr释放实验来检测动物的细胞免疫活性 ,IL - 2 -TNFα和B7 1共同作用诱导了比其中的任意一个单独作用更强的抗肿瘤应答 (比IL - 2 -TNFα高 2 5% ,比B7 1高 2 0 % )。结论 IL - 2 -TNFα和B7 1协同作用可提高他们所修饰的肿瘤细胞的疫苗效力。

Objective: To study the vaccine potency of gene-modified tumor cells, we have constructed IL-12, H7-1 and GM-CSF express vector using retrovirus. Methods: It was transfected into EL-4 thymic lylmphoma cells respectively and the effect of gene transduction on anti-tumor immunity were investigated. Results: The tumorigenicity of EL-4/IL-12 transfectant in C57BI/6 syn- ergistical mice was decreased significantly after implanted with EL-4/IL-12 transfectant compaired with EL-4/Wt or EL-4/Neo...

Objective: To study the vaccine potency of gene-modified tumor cells, we have constructed IL-12, H7-1 and GM-CSF express vector using retrovirus. Methods: It was transfected into EL-4 thymic lylmphoma cells respectively and the effect of gene transduction on anti-tumor immunity were investigated. Results: The tumorigenicity of EL-4/IL-12 transfectant in C57BI/6 syn- ergistical mice was decreased significantly after implanted with EL-4/IL-12 transfectant compaired with EL-4/Wt or EL-4/Neo groups (P<0. 01). The systemic protective immunity was induced against the challenge with EL-4/Wt (in 10/15 mice) after the rejection of EL-4/IL-12 in the experiment, a stronger CTL activity against EL-4/Wt cells and a weak killer activity against syn- geneic Lewis Lung Carcinoma cells were obtained in 51Cr release assay. In vivo depletion analysis suggested that the decreased tumorigenicity mainly depended on CD4+, CD8+ and NK cells. Therapeutic vaccines with EL-4/IL-12 cells could retard the growth to estabished EL-4/Wt tumors significantly compared with those of EL-4/Neo(P < 0 .005 ), combination of therapeutic vaccines of EL-4/IL-12 and EL-4/B7-1 result in the enhanced the therapeutic effect of each single transficants (P < 0 .005) in this experimental model. Conclution: These studies suggested that immunogene treatment using IL-12 is effective in hematopoi- etic malignancy, and combination of IL-12 with B7-1 have a aplication value in human cancer treatment in the near future.

目的:我们应用逆转录病毒构建了IL-12,R7-1和GM-CSF表达载体,以研究基因修饰的肿瘤细胞的癌疫苗作用。方法:将3种表达载体分别转染EM胸腺瘤细胞并研究了该基因导入细胞的抗肿瘤免疫效果。结果:当接种了EL-4/IL-12细胞后,在C57BL/6同系鼠中其基因导入细胞的肿瘤原性比较EL-4和EL-4/Neo组明显减少(P<0.01)。在EL-4/IL-12被排斥后,体内试验中诱发了实验动物抗 EL-4/Wt的系统性、保护性免疫,51Cr释放测定中,获得一个较强的抗EL-4/Wt和一个较弱的抗同系Lewis肿瘤细胞的CTL活性,体内淋巴细胞消除分析的结果提示减少的肿瘤原性主要依赖于CD4+,CD8+和NK细胞。用EL-4/IL-12细胞进行疫苗治疗比较用EL-4/Neo细胞能有效地延缓已建立的EL-4/Wt肿瘤的生长(P<0.005),EL-4/IL-12和EL-4/B7-1联合比用单一的转基因细胞增强了治疗效果(P<0.005)。结论:提示应用IL-12进行血液肿瘤的治疗是有效的,IL-12和B7-1联合使用在未来人类癌症的治疗中亦可有一定的应用前景。

 
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