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seed virus
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  “seed virus”译为未确定词的双语例句
     Objective To explore the gene changes of the mumps vaccine S79 strain during the successive 12 passages,and compare the sequence among the three viruses:S79 master seed virus(S79-1-3),S79 plaque-purified strain(S79-1-P7-3③)and ME (Enders) strain, a mumps virus strain cultivated in chicken embryonic allantoic fluid,in the laboratory.
     目的观察流行性腮腺炎(腮腺炎)病毒(MuV)S79株各代病毒的蛋白基因在传代过程中是否发生变化,对比S79株主代病毒(S79-1-3)、蚀斑纯化病毒(S79-1-P7-3③)及ME(Enders株)病毒的基因差异。
短句来源
     These results indicate that the high growth H5/PR8 reassortant has properties that are desirable in a vaccine seed virus and is suitable for use in the event of a H5 pandemic.
     实验结果表明,已获得一株高繁殖力的重组流感病毒H5/PR8,血凝价达到211,生长繁殖特性明显增强。 抗原性分析显示,H5/PR8重组病毒与亲本毒株H5N2抗原性无明显差异;
短句来源
     For studing the structure and characteristic of nucleoprotein (NP) of Seoul virus R22strain, which is the seed virus of HFRS candidate vaccine, the NP gene coding region of the R22S segment was amplificated by RT-PCR and then cloned it into pET-3a expression plasmid.
     为研究肾综合征出血热病毒疫苗侯选株R22核蛋白的结构与特性,应用逆转录PCR扩增了R22编码区基因,并将扩增产物克隆于pET-3a表达质粒,测得R22株S片段编码区序列为1290个核苷酸。
短句来源
     Molecular Characteristics of the Seed Virus of HFRS Candidate Vaccine A16 Strain
     肾综合征出血热疫苗候选毒株A16株的分子特性
短句来源
     It was identified that chicken(Gallus domesticus)embryo lethal virus(CELOV)polluted duck viral hepatitis seed virus of chicken embryonic allantochoroin liquid by PCR.
     通过随机引物 PCR 鉴定了鸡(Gallus domesticus)胚孤儿致死病毒(CELOV)污染鸭肝炎病毒鸡胚尿囊液种毒。
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  相似匹配句对
     SEED TRANSMISSION OF SOYBEAN MOASAIC VIRUS
     大豆花叶病毒种子传染的研究
短句来源
     The master virus seed was adopted for challenge.
     用于效力检验的攻毒毒种采用原始毒种。
短句来源
     Virus counter
     病毒计数器
短句来源
     (e) infection by virus;
     病毒说:细菌感染或多病原说;
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     The seed of C.
     田野菟丝子C .
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  seed virus
When a certain dilution of seed virus was used constantly, a fairly high reproducibility of serum endpoints was demonstrated, which were comparable to those obtained by other routine neutralization methods.
      
A linear relationship between incubation period (time after inoculation of virus before cytopathic effect) and log concentration of seed virus has been demonstrated for some adenoviruses in tissue culture.
      
Live attenuated homologous vaccine against peste des petits ruminants of sheep and goats was produced on a large scale basis in roller culture bottles using seed virus developed at the Indian Veterinary Research Institute, Muktheswar, India.
      
The seed virus was prepared for each strain by growing in C6/36 to high titer exceeding 108 PFU/ml.
      
The recombinant clone is directly used for the preparation of the Master Seed virus.
      
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Dogs were immunized orally with an attenuated rabies vaccine, No.22 strain on mice brain.The liquid vaccine was placed in rice porridge,which is acceptable to and readily ingested by the animals.Serological response was tested with mice serum neutralization test.After immunization seroconversion rates in seronegative dogs were 100% (GMT 42, N=20), 91.7%(GMT76, N=12)and 80% (GMT34,N=5) in groups receiving oral vaccine after 1,6 and 15 months respectively.These results indicate that the attenuated oral rabies...

Dogs were immunized orally with an attenuated rabies vaccine, No.22 strain on mice brain.The liquid vaccine was placed in rice porridge,which is acceptable to and readily ingested by the animals.Serological response was tested with mice serum neutralization test.After immunization seroconversion rates in seronegative dogs were 100% (GMT 42, N=20), 91.7%(GMT76, N=12)and 80% (GMT34,N=5) in groups receiving oral vaccine after 1,6 and 15 months respectively.These results indicate that the attenuated oral rabies vaccine virus was highly immunogenic,genetically stable,and safe for dogs.According to the data mentioned above,it is considered that the No. 22 virus strain could be used as a seed virus for ths production of live attenuated rabies vaccine for dogs.

用我国分离并传代适应的狂犬病病毒固定毒22号毒株作为家犬口服疫苗毒株。22号毒株活毒疫苗用于家犬一次口服(拌入煮熟待凉的粥内,在口腔停留约30秒至2分钟服完)即可获得免疫。免疫力可持续15个月,至少与国外Flury LEP疫苗相同。流行病学观察19,425只家犬证明疫苗安全、有效。犬用口服疫苗在我国属首创,国外仅有用于狐狸的口服疫苗,用于家犬的同类制品未见报道。

Abstract Egg Drop Syndrome 1976 (EDS-76) virus was rapidly detected by plate hemagglutination (HA) test for quantitative analysis and by micro-HA test for quantitative determination. Different shapes occured in plate HA test were corelated with the virus titers obtained from micro-HA test.When virus were able to completely agglutinate an equal volume of 10% chicken erythrocyle suspension and the agglutinated shapes were obvious in plate test at 10~25℃ within 30 seconds, their geometric mean...

Abstract Egg Drop Syndrome 1976 (EDS-76) virus was rapidly detected by plate hemagglutination (HA) test for quantitative analysis and by micro-HA test for quantitative determination. Different shapes occured in plate HA test were corelated with the virus titers obtained from micro-HA test.When virus were able to completely agglutinate an equal volume of 10% chicken erythrocyle suspension and the agglutinated shapes were obvious in plate test at 10~25℃ within 30 seconds, their geometric mean titers (GMT) of micro-HA test were more than 5×212.If the agglutinations of the samples were not apparent or needed more than 2 minutes to occur in plate test, their micro-HA titers were less thans×210. The agglutination of the samples could be specially inhibited by EDS-76 antisera. This method is considered suitable for a rapid initial screening system to get higher titer viruses especially for selection of seed viruses from a large number of original samples.

用平板红细胞凝集(HA)试验对鸡产蛋下降综合症(EDS-76)病毒进行了快速定性检测,并与微量HA试验进行了对比。结果表明,当病毒HA滴度在5×212及其以上时,在10~25℃范围内于半分钟可与10%鸡红细胞(RDC)发生极明显的大片凝集。当病毒HA滴度在5×210以下时,则凝集片明显较小,且反应时间在2分钟以上。这种凝集现象可被EDH-76阳性血清特异性抑制。此法可用于对大批量样品中高效价病毒,特别是种毒的初步选筛。

Inactivated oil emulsion vaccine against ND was studied and prepared successfully,by using NDV Lasota strain as seed virus,formalin as inactivating agent,No 7 white mineral oil as adjuvant, Tween-80 and span-80 as emulsifiers, merthiolate as preservative. This oil-emulsion vaccine, of a double emulsion type (W/O/W), had particles about 1μm in diameter. The vaccine was thin emulsion with the viscosity≤1 second,it was easy to inject. Under 4--8℃,the vaccine can be preserved for over one year. Potency tests...

Inactivated oil emulsion vaccine against ND was studied and prepared successfully,by using NDV Lasota strain as seed virus,formalin as inactivating agent,No 7 white mineral oil as adjuvant, Tween-80 and span-80 as emulsifiers, merthiolate as preservative. This oil-emulsion vaccine, of a double emulsion type (W/O/W), had particles about 1μm in diameter. The vaccine was thin emulsion with the viscosity≤1 second,it was easy to inject. Under 4--8℃,the vaccine can be preserved for over one year. Potency tests indicated the dosage was ≥70PD50 The earliest immunity was produced at 12 days after inoculation (100% prortection ). Practically, the preferred dosage was 0. 5ml per bird for birds within 2 months of age, and 1. 0ml per bird for the birds older than 2 months of age. The vaccine was safe for chickens of any ages, and had no adverse effects. When combined with live ND vaccines, it could induce high HI antibody for chicks of any ages. Immune periods lasted 2. 5 to 3 months for chickens and more than 6 months for adult birds. The viscerotropic velogenic ND and atypical ND can be prevented effectively by using this vaccine.

采用NDLasota株为种毒,福尔马林为灭活剂,7号白油为佐剂,吐温80、司本80为乳化剂,硫柳汞为防腐剂,高压匀浆泵为乳化工具,研制出ND油性剂灭活疫苗。其剂型为双相型(W/O/W),呈稀薄乳状液,粒度为1μm左右,粘度≤1s,通针性好。4~8℃有效保存期1年。效力≥70PD50,最早产生免疫时间为12天(100%保护),生产中常用免疫剂量为2月龄内每只0.5ml,2月龄以上每只1.0ml皮下注射,对各日龄鸡安全无副作用。与ND活毒疫苗同时免疫可使各日龄鸡产生高效价的HI抗体,雏鸡免疫期为2.5~3个月,成年鸡为12个月。此苗可有效地预防强毒力嗜内脏型ND及“非典型”ND。

 
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