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colony forming cells
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  集落形成细胞
     Transduction efficiency (20.5%) in the presence of bone marrow stromal cells plus cytokines(SCF+FL+IL 3) was higher than that (15.2%) without stromal cells, and the number of drug resistant colony forming cells was more in the former than in the latter.
     骨髓基质细胞 +细胞因子 (SCF +FL +IL 3)支持的基因转导效率 (2 0 .5 % )又高于单纯用该组细胞因子的转导效率 (15 .2 % ) ,并且前者形成的抗性集落形成细胞数多于后者。
短句来源
     Objective:To evaluate the changes of colony forming cells with high proliferative potential (HPP CFC) in human CD34 + cell culture after in vitro amplification.
     目的 :评价CD34 +细胞体外扩增时高增殖潜能集落形成细胞 (HPP CFC)的变化。
短句来源
     Objective:To investigate the colony formation capacity and P16 gene mRNA expression of high proliferative potential colony forming cells (HPP-CFC) derived from bone marrow hematopoietic cells of psoriatic patients,and probe into the relationship between the colony formation and P16 promoter mRNA expression.
     目的:研究银屑病患者骨髓高增殖潜能集落形成细胞(HPP-CFC)的集落形成能力及P16基因mRNA表达,探讨银屑病患者骨髓造血干细胞体外增殖活性及原因。
短句来源
     Aim and Methods: High proliferative potential colony forming cells (HPP CFC) as one of the most primitive hematopoietic progenitors with CD34 + DR - Lin - antigens have been identified and used as effective tools for examination of functional properties of hematopoietic stem cells.
     目的和方法:高增殖潜能集落形成细胞(HPPCFC)是表达CD34+DRLin-的最早期造血祖细胞之一,它在体外的增殖分化能力可反映造血干细胞的某些特征。
短句来源
     It has been shown thatUCB contains abundant hematopoietic stem/progenitor cells, the content ofCD34~CD38 and the numbers of colony forming cells and long-termculture-initialing cells are much greater in UCB than in BM and MPB fromadults.
     这是因为脐血中含有丰富的造血干/祖细胞:CD34~+CD38~-细胞、集落形成细胞(CFC)、长期培养启动细胞(LTC-IC)的含量均高于骨髓和动员外周血。
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  克隆形成细胞
     Results:Circulating CD 34 + Thy 1 + cells,CD 34 + cells and colony forming cells increased by 48.6 ,50 and 53.1 fold, respectively,after mobilization. The peak levels were reached at 12~14 days after chemotherapy.
     结果:动员后循环血中CD34+Thy-1+细胞、CD34+细胞和克隆形成细胞(CFC)含量分别增高48.6倍、50.0倍和53.1倍,高峰时间在化疗后第12~14天(注射G-CSF的第6~8天);
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  “colony forming cells”译为未确定词的双语例句
     Mutation frequency in 106 colony forming cells was increased from 20.4 ± 6.7 at 20 mJ/cm2 to 97.7 ± 7.1 under 60 mJ/cm2 of UVB irradiation. The expression level of ATP mRNA was down-regulated after UV irradiation.
     在每106个突变克隆细胞中突变频率由20mJ/cm2的(20.4±6.7)个增加到60mJ/cm2的(97.7±7.1)个。
短句来源
     The order of the sensitivity of these colony forming cells from high to low was CFU L,CFU GM,CFU Blast and CFU F(P<0.01).
     它们对各种集落形成细胞的杀伤力从大到小依次为CFU-L、CFU-GM、CFU-Blast和CFU-F(P<0.01)。
短句来源
     RESULTS: The mesenchymal stem cells and cytokines co-culture systems significantly enhanced the expansion of the cord blood cells with the increase of the total number of nucleated cells by 114.2±2.4-fold, colony forming cells by 40.5±8.6-fold and CD34 + cells by 11.3±0.4-fold, respectively.
     结果 :MSCS 联合细胞因子组较其他组培养体系对有核细胞总数、CFCs含量及CD34+ 细胞含量均具有明显的扩增作用 ,分别扩增了 114 .2± 2 .4 ,4 0 .5± 8.6 ,11.3± 0 .4倍 .
短句来源
     Study of Fibroblast Colony Forming Cells in 57 Cases of Leukemia and Their Clinical Significance
     57例白血病成纤维细胞集落的形成及其临床意义的探讨
短句来源
     CD 34 - cell population from cord blood MNC contained hematopoietic stem/progenitor cells and the cytokines secreted by CD 34 - cells could induce CD 34 + cells to more mature colony forming cells.
     脐血MNC中大量的CD34- 细胞含有造血干 祖细胞 ,其分泌的细胞因子有促进CD34+ 细胞向较为成熟的集落形成祖细胞分化的作用。
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  colony forming cells
In the present studies all of the 5 different human glioma cell lines tested with DFMO treatment also showed enhanced uptake of exogenous [3H] putrescine, reduced cell counts and enhanced killing of colony forming cells (CSF).
      
Although spirogermanium has no effect on normal bone marrow colony forming cells in mice, dogs, or man, it has revealed cytotoxic activity in vitro against human myeloid leukemia cell line K 562 at clinically achievable concentrations.
      
Curettage was shown to remove about half of the fibroblast colony forming cells (FCFC) from the bone marrow.
      
Effect of lithium on normal and chronic granulocytic leukemia colony forming cells (CFU-GM)
      
The influence of a new antitumor agent on hemopoietic colony forming cells
      
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Effects of five antitumor drugs on the murine normal hemopoietic and P_(388) leukemic colony-forming cells were studied and compared by means of spleen colony assay. Dose-survival curves for the normal spleen colony-forming units (NCFU-S) and the leukemic spleen colony-forming units (LCFU-S) exposed to harringtonine (H), partial synthetic harringtonine (PSH), homoharringtonine (HH), and cyclophosphamide (CP)exhibited exponential forms. The value of efficacy ratios...

Effects of five antitumor drugs on the murine normal hemopoietic and P_(388) leukemic colony-forming cells were studied and compared by means of spleen colony assay. Dose-survival curves for the normal spleen colony-forming units (NCFU-S) and the leukemic spleen colony-forming units (LCFU-S) exposed to harringtonine (H), partial synthetic harringtonine (PSH), homoharringtonine (HH), and cyclophosphamide (CP)exhibited exponential forms. The value of efficacy ratios of the four drugs was 5.15, 6.01, 7.96, and 9.98 in the following order for H, PSH, HH, and CP, respectively. All dosage levels of arabinosylcytosine (Ara-C)used showed no distinct effect on the NCFU-S, but exhibited a strong effect on the LCFU-S with increasing dosage; the dose-survival curve for LCFU-S was decreased to a plateau value of 0.2% at the dosage of 500-1000 mg/kg. No obvious effect of H, HH, and PSH on the dose-survival curves was observed when the doses used were less than 0.3, 0.28 and 0.75 mg/kg, respectively.

用脾集落形成法比较了5种抗癌药对小鼠造血干细胞(NCFU-S)和P_(388)白血病干细胞(LCFU-S)的作用。三尖杉酯碱、半合成三尖杉酯碱、高三尖杉酯碱和环磷酰胺对两类干细胞作用的剂量—反应曲线呈指数形,效能比依次为5.15,6.01,7.96和9.98。阿糖胞苷对NCFU-S无明显作用,但对LCFU-S杀伤作用强,大剂量时剂量—反应曲线趋于水平。当三尖杉酯碱、高三尖杉酯碱和半合成三尖杉酯碱的剂量分别低于0.30,0.28和0.75mg/kg时,对LCFU-S无明显作用,剂量—反应曲线上出现“肩形”,提示LCFU-S在小剂量三尖杉酯类生物碱作用下,有一受亚致死性损伤后修复过程。

By using single spleen colony transplantation technique and sex chromosome typing as natural cytogenetic markers, we have been able to demonstrate that most spleen colony forming cells in the adult bone marrow or in foetal liver of inbred LACA or C57 mice possess the potential of re-establishment of myeloid and lymphoid lines of cells in lethally irradiated mice. However, most of the spleen colony forming cells in the peripheral [blood of normal mice possess weak or no potentiality to proliferate...

By using single spleen colony transplantation technique and sex chromosome typing as natural cytogenetic markers, we have been able to demonstrate that most spleen colony forming cells in the adult bone marrow or in foetal liver of inbred LACA or C57 mice possess the potential of re-establishment of myeloid and lymphoid lines of cells in lethally irradiated mice. However, most of the spleen colony forming cells in the peripheral [blood of normal mice possess weak or no potentiality to proliferate or re-establish hemopoiesis in lethally irradiated mice. Our results support the notion that the CFU-S population in mice is heterogeneous.From the nature of colony forming cells in the peripheral blood of normal mice, we are convinced that the assessment of CFU-S content relying upon the spleen colonies as the unique sign to indicate the self-renewal ability of a spleen colony forming cell is by no means valid inasmuch as some plu-ripotent . hemopoietic progenitors derived from pluripotent hemopoietic stem cells may have the same ability to form colonies in vivo.

应用单个脾结节和性染色体作为天然的细胞遗传学标志,证明存在于纯系LACA或C_(57)胎鼠肝脏和成年小鼠骨髓中的脾结节生成细胞(简称CFU-S)大多具有重建照射小鼠髓系与淋巴系细胞的功能。因而,是一类淋巴-髓系干细胞。然而,存在于正常成年小鼠外周血中的CFU-S,虽然它们也可以在照射小鼠脾脏上生成由各系血细胞组成的脾结节,但其中至少有一部分已经失去了重建照射小鼠造血功能的能力,反映了CFU-S在功能上的不均一性。脾结节测试技术是目前测定造血干细胞的一项重要技术,但是,能够生成脾结节的细胞,不一定都具有造血干细胞的基本特性,一些由造血干细胞分化的多向性造血祖细胞也可能在照射的小鼠脾脏上生成脾结节。

By using spleen colony transplantation technique and sex chromosome as acytogenetic marker we demonstrated that in (C57×LACA) F_1 mice most ofthe spleen colony forming cells in bone marrow possess very weak potential in re-establishment of hemopoietic and immunological functions in lethally irradiatedisogeneic recipients. After scheduled injections of hydroxyurea the percentageof spleen colony forming cells with great potential in reconstitution of both myeloidand lymphoid lines of cells in...

By using spleen colony transplantation technique and sex chromosome as acytogenetic marker we demonstrated that in (C57×LACA) F_1 mice most ofthe spleen colony forming cells in bone marrow possess very weak potential in re-establishment of hemopoietic and immunological functions in lethally irradiatedisogeneic recipients. After scheduled injections of hydroxyurea the percentageof spleen colony forming cells with great potential in reconstitution of both myeloidand lymphoid lines of cells in lethally irradiated mice was greatly increased. Thisfact shows that in normal F_1 mice, at least two subsets of spleen colony formingcells are co-existing, one with high potential in self-replication (P_s-CFU_s), theother with low potential in self-replication (P_g-CFU_s).

应用单个脾结节移植和性染色体追踪方法证明正常F_1小鼠骨髓脾结节生成细胞(CFCs)的造血重建能力是很不均一的,连续注射羟基脲(HU)可使具有造血重建能力的脾结节生成细胞的比例显著提高。由此提出,小鼠造血组织中同时存在着具有多向性造血干细胞特性的脾结节生成细胞(Ps-CFUs)和具有多向性造血祖细胞特性的脾结节生成细胞(Pg-CFUs)。注射HU可使Pg-CFUs被杀灭,Ps-CFUs 相对比例增加;或者由于大量 CFU-S被杀灭,脾结节生成细胞的前体细胞(Pre-CFUs)加速向 Ps-CFUs分化。因此血细胞的生成过程中可能经历了以下细胞分化和成熟的阶段 Pre-CFUs→Ps-CFUs→Pg-GFUs→各系祖细胞→各系血细胞。

 
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