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   oral inoculation 在 消化系统疾病 分类中 的翻译结果: 查询用时:0.597秒
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oral inoculation
相关语句
  口服接种
    AIM: To investigate the influence of oral cavity and gastrointestinal tract environment on the biological behavior of recombinant Bacillus Calmette-Guerin (rBCG) that expresses with allergen Der p2 after oral inoculation.
    目的:观察口服接种过敏原基因(Der p2)重组(r)BCG口咽胃肠道环境对Der p2-rBCG行为的影响.
短句来源
  口服免疫
    CONCLUSION: It is suggested that the oral cavity and gastrointestinal tract environment does not interrupt rBCG expressed Der p2 from inducing immune response after oral inoculation.
    结论:rBCG口服免疫后口咽胃肠道环境对rBCG生物学行为所产生的影响并不妨碍诱导理想的免疫应答.
短句来源
  “oral inoculation”译为未确定词的双语例句
    Methods UC was induced in mice with oral inoculation of DSS for seven days.
    方法 用 5%葡聚糖硫酸钠(DSS)口饲法制备小鼠UC模型 ;
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  oral inoculation
These data are important because they indicate that the antigenic load achieved following a single oral inoculation is sufficient to achieve long lasting immunity, the goal of any potential vaccine.
      
Pups born to dams immunized by oral inoculation with live MHV acquired both MHV-specific IgA and IgG in their whey, while pups born to dams immunized with killed virus acquired only IgG.
      
The in vivo dose study (1, 10, 25, 50 μg of OVA) was performed by subcutaneous and oral inoculation in mice by single (0 week) or double (0 and 3 weeks) administration of PLGA 50/50 microspheres containing 0.1% OVA.
      
Oral inoculation of strains lacking the 55 kb plasmid did not cause any mortality.
      
In order to determine if intra-oral inoculation could explain the pathogenesis of paracoccidioidomycosis, 64 BALB/c mice were inoculated intra-orally with 850.000 viable P.
      
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Objective To investigate the effects of Bifido bacterium on the expression of cytokines and activation of nuclear factor κB in murine intestinal mucosa with ulcerative colitis(UC). Methods UC was induced in mice with oral inoculation of DSS for seven days. The expression of TNF-α, IL-1β in the intestinal mucosa of mice were detected by a semi-quantitative assay, RT-PCR. The activation of NF-κB in the intestina l mucosa were evaluated by electrophoretic mobility shift assay (EMSA). The rele vance of oral...

Objective To investigate the effects of Bifido bacterium on the expression of cytokines and activation of nuclear factor κB in murine intestinal mucosa with ulcerative colitis(UC). Methods UC was induced in mice with oral inoculation of DSS for seven days. The expression of TNF-α, IL-1β in the intestinal mucosa of mice were detected by a semi-quantitative assay, RT-PCR. The activation of NF-κB in the intestina l mucosa were evaluated by electrophoretic mobility shift assay (EMSA). The rele vance of oral inoculation of Bifidobacterium and the expression of cytokines and the activation of NF-κB were analyzed. Results Com paring to the mice treated with DSS alone, both symptoms and the lesions of colo nic mucosa were slighter in the animals orally inoculated with Bifidobacterium during the induction of colitis than that of control. Furthermore, the expres sions of TNF-α, IL-1β were significantly decreased(P<0.05). The activ ation of NF-κB was also suppressed. Conclusion Bifido bacterium had both prophylaxis and therapeutic effects on UC, that was probabl y due to the suppression of the activity of NF-κB and the subsequent inhibiton of the expression of proinflammatory cytokines.

目的 建立小鼠溃疡性结肠炎 (UC)动物模型 ;应用双歧杆菌活制剂 ,观察其对UC发生、发展的影响 ,并检测其对TNF α、IL 1β的表达和NF κB活性的影响。方法 用 5%葡聚糖硫酸钠(DSS)口饲法制备小鼠UC模型 ;给小鼠口服双歧杆菌 (Bifidobacterium ,Bf)活菌 ,观察UC的症状和组织学变化 ;采用逆转录 聚合酶链反应 (RT PCR)技术 ,检测UC小鼠肠黏膜内TNF α、IL 1β的表达水平进行半定量测定 ;应用免疫电泳迁移率改变分析 (electrophoreticmobilityshiftassay ,EMSA)法检测UC小鼠肠黏膜细胞NF κB的激活。结果 应用双歧杆菌制剂后 ,UC的症状、组织损害均较模型组明显减轻。同时TNF α、IL 1β的表达较单纯模型组降低 (P <0 .0 5) ,NF κB的核结合活性也降低 ,但较正常对照组仍明显增加。结论 生态制剂Bf对UC的发生有预防作用 ,可能是通过抑制NF κB的核结合活性 ,进而降低致炎细胞因子的表达完成的

AIM: To investigate the influence of oral cavity and gastrointestinal tract environment on the biological behavior of recombinant Bacillus Calmette-Guerin (rBCG) that expresses with allergen Der p2 after oral inoculation. METHODS: Four groups of 6 to 8 week old Balb/c mice were vaccinated orally with 100 μL of 109CFU with natural BCG or three kinds of rBCGs. The BCG CFU was counted in several tissue (oral pharyngeal lymphoid tissues (OLT), gut associated lymphoid tissues (GALT), spleen, lung and...

AIM: To investigate the influence of oral cavity and gastrointestinal tract environment on the biological behavior of recombinant Bacillus Calmette-Guerin (rBCG) that expresses with allergen Der p2 after oral inoculation. METHODS: Four groups of 6 to 8 week old Balb/c mice were vaccinated orally with 100 μL of 109CFU with natural BCG or three kinds of rBCGs. The BCG CFU was counted in several tissue (oral pharyngeal lymphoid tissues (OLT), gut associated lymphoid tissues (GALT), spleen, lung and liver) and feces from the inoculated Balb/ c mice at different time after inoculation. The DNA and mRNA of Der p2 gene in various tissue were identified by PCR and RT-PCR, respectively. RESULTS: All three kinds of rBCGs were observed in mu-cosal-associated lymphoid tissues, indicating that they could penetrate the mucosa of oral cavity and gastrointestinal tract. The Der p2 gene was expressed by rBCG in above tissues. CONCLUSION: It is suggested that the oral cavity and gastrointestinal tract environment does not interrupt rBCG expressed Der p2 from inducing immune response after oral inoculation.

目的:观察口服接种过敏原基因(Der p2)重组(r)BCG口咽胃肠道环境对Der p2-rBCG行为的影响. 方法:以野生BCG为对照,给予Balb/c小鼠口服接种三种方式(分泌、胞壁和胞内)表达Der p2的rBCG1×109CFU,连续5 d,计数不同时间粪便、口咽淋巴组织(OLT)、消化道相关淋巴组织(GALT)以及肝脏、脾脏和肺脏中BCG的细菌数;用PCR和内参照RT—PCR法测定由rBCG携带入各组织中的Der p2存在和表达状况. 结果:三种rBCG均可通过口咽和胃肠道黏膜,进入黏膜相关淋巴组织;各淋巴组织中的rBCG仍能表达其所携带的外源基因,并在各淋巴组织中表达外源蛋白. 结论:rBCG口服免疫后口咽胃肠道环境对rBCG生物学行为所产生的影响并不妨碍诱导理想的免疫应答.

AIM: To detect the expression of Cyclin D1 and proliferating cell nuclear antigen (PCNA) in liver tissues of C57BL/6 mice infected with H pylori by oral inoculation. METHODS: Thirty C57BL/6 strain mice, used as experiment animal, were orally inoculated with H pylori SS1 strain and fed in laminar flow cabinets for 8 mo. H pylori 16S rRNA in liver was examined by nested polymerase chain reaction (PCR), and then mRNA and protein were extracted from the positive liver tissues. The mRNA and protein expression...

AIM: To detect the expression of Cyclin D1 and proliferating cell nuclear antigen (PCNA) in liver tissues of C57BL/6 mice infected with H pylori by oral inoculation. METHODS: Thirty C57BL/6 strain mice, used as experiment animal, were orally inoculated with H pylori SS1 strain and fed in laminar flow cabinets for 8 mo. H pylori 16S rRNA in liver was examined by nested polymerase chain reaction (PCR), and then mRNA and protein were extracted from the positive liver tissues. The mRNA and protein expression of Cyclin Dl and PCNA were detected by reverse transcription PCR (RT-PCR) and Western blot, respectively. RESULTS: Six of fifteen liver tissues were positive for H pylori 16S rRNA after examination of nested PCR. Sequencing results of 16S rRNA PCR products showed the 100% homogeneity with cultured H pylori from gastric mucosa and inoculated H pylori SS1. The mRNA expression of Cyclin D1 and PCNA in liver of C57BL/6 mice infected with H pylori were significantly increased in comparison with those in the controls (0.78±0.13 vs 0.66±0.03, P < 0.05; 0.86 ±0.17 vs 0.56±0.24, P < 0.01), and the protein expression of PCNA was also increased (1.16 ±0.40 vs 0.64±0.11, P < 0.05). Although the expression of Cyclin D1 protein had an increased tendency, it was not significantly different from those in the controls (P > 0.05). CONCLUSION: H pylori inoculated orally can arrive at liver, and induce increased expression of Cyclin Dl and PCNA.

目的:探讨经口接种制作的幽门螺杆菌(H pylori)小鼠感染模型H Pylori对肝组织Cyclin D1和PCNA表达的影响.方法:C57BL/6小鼠30只经口感染接种H pylori悉尼株(SS1),置层流柜中饲养8 mo.用巢式PCR检测肝组织H pylori 16S rRNA,并提取阳性样本的mRNA与蛋白,用RT-PCR、 Western blot等方法检测小鼠肝组织癌基因 Cyclin D1和PCNA mRNA及蛋白表达变化.结果:巢式PCR检测15只小鼠肝组织中H pylori DNA,有6只16S rRNA基因为阳性,测序结果经序列分析后显示与胃黏膜分离培养细菌、接种细菌同源性100%.肝组织内有 H pylori定植的6只感染小鼠肝组织Cyclin D1 和PCNA mRNA表达升高(0.78±0.13 vs 0.66 ±0.03,P<0.05:0.86±0.17 vs 0.56±0.24, P<0.01).Western blot结果显示,Cyclin D1蛋白表达有增高趋势,但与对照组比较无显著性差异;PCNA蛋白表达增高,与对照组比较有显著性差异(1.16±0.40 vs 0.64±...

目的:探讨经口接种制作的幽门螺杆菌(H pylori)小鼠感染模型H Pylori对肝组织Cyclin D1和PCNA表达的影响.方法:C57BL/6小鼠30只经口感染接种H pylori悉尼株(SS1),置层流柜中饲养8 mo.用巢式PCR检测肝组织H pylori 16S rRNA,并提取阳性样本的mRNA与蛋白,用RT-PCR、 Western blot等方法检测小鼠肝组织癌基因 Cyclin D1和PCNA mRNA及蛋白表达变化.结果:巢式PCR检测15只小鼠肝组织中H pylori DNA,有6只16S rRNA基因为阳性,测序结果经序列分析后显示与胃黏膜分离培养细菌、接种细菌同源性100%.肝组织内有 H pylori定植的6只感染小鼠肝组织Cyclin D1 和PCNA mRNA表达升高(0.78±0.13 vs 0.66 ±0.03,P<0.05:0.86±0.17 vs 0.56±0.24, P<0.01).Western blot结果显示,Cyclin D1蛋白表达有增高趋势,但与对照组比较无显著性差异;PCNA蛋白表达增高,与对照组比较有显著性差异(1.16±0.40 vs 0.64±0.11,P<0.05).结论:经口接种H Pylori可达小鼠肝脏,使 Cyclin D1和PCNA表达增强.

 
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