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bone marrow mononuclear cell
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  骨髓单个核细胞
     Methods:Researched cases of acute nonlymphoblastic leukemia(ANLL) were divided into two groups:M1-M2 subtype and M3 subtype. Flow cytometery(FCM) was used to detect the rates of positive expression of CD117 and CD34 on bone marrow mononuclear cell(BMMNC) in 54 patients of M3 and 63 patients of M1-M2 subtype respectively.
     方法:将研究病例分为M1~M2和M3二组,采用流式细胞术(F low cytom etery,FCM)随机检测54例M3和63例M1~M2二组初诊患者骨髓单个核细胞(BMMNC)髓系抗原CD117和干(祖)细胞抗原CD34的表达;
短句来源
     Methods: Expressions of PTTG and c-myc gene mRNA were detected in bone marrow mononuclear cell (BMMNC) from 34 patients with CML(16 chronic phase, 11 accelerated phase and 7 blast crisis) and 10 controls by reverse transcriptase-polymerase chain reation (RT-PCR).
     方法:采用RT-PCR方法检测34例CML患者(慢性期16例,加速期11例,急变期7例)及10例对照者骨髓单个核细胞(BMMNC)中PTTG和c-myc基因的表达。
短句来源
     Expression of PTTG mRNA was detected in bone marrow mononuclear cell (BMMNC) from 32 cases with MDS and 10 normal controls by reverse transcriptase-polymerase chain reaction (RT-PCR).
     [方法]采用RT鄄PCR方法检测32例MDS患者及10例正常骨髓单个核细胞(bonemarrowmononuclearcell,BMMNC)中PTTGmRNA的表达。
短句来源
     Results After treatment with FITC-labeled G3139 at the concentration of 0 60 μmol·L -1 for 4 h, the G3139 uptake into peripheral blood mononuclear cell and bone marrow mononuclear cell in hematological tumor patients was significantly higher than that in normal control.
     结果 经过 0 60 μmol·L-1 荧光素FITC标记的G31 39作用 4h后 ,血液系统肿瘤患者外周血及骨髓单个核细胞对G31 39的摄取能力明显高于正常人 ;
短句来源
     Methods Expression of MPO was detected in bone marrow mononuclear cell(BMMNC) from 36 cases with ANLL and 30 normal controls by capillary electrophoresis (CE).
     方法采用毛细管电泳(CE)电化学检测法检测36例ANLL患者及30例正常骨髓单个核细胞(BMMNC)中MPO表达。
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  骨髓单个核细胞
     Methods:Researched cases of acute nonlymphoblastic leukemia(ANLL) were divided into two groups:M1-M2 subtype and M3 subtype. Flow cytometery(FCM) was used to detect the rates of positive expression of CD117 and CD34 on bone marrow mononuclear cell(BMMNC) in 54 patients of M3 and 63 patients of M1-M2 subtype respectively.
     方法:将研究病例分为M1~M2和M3二组,采用流式细胞术(F low cytom etery,FCM)随机检测54例M3和63例M1~M2二组初诊患者骨髓单个核细胞(BMMNC)髓系抗原CD117和干(祖)细胞抗原CD34的表达;
短句来源
     Methods: Expressions of PTTG and c-myc gene mRNA were detected in bone marrow mononuclear cell (BMMNC) from 34 patients with CML(16 chronic phase, 11 accelerated phase and 7 blast crisis) and 10 controls by reverse transcriptase-polymerase chain reation (RT-PCR).
     方法:采用RT-PCR方法检测34例CML患者(慢性期16例,加速期11例,急变期7例)及10例对照者骨髓单个核细胞(BMMNC)中PTTG和c-myc基因的表达。
短句来源
     Expression of PTTG mRNA was detected in bone marrow mononuclear cell (BMMNC) from 32 cases with MDS and 10 normal controls by reverse transcriptase-polymerase chain reaction (RT-PCR).
     [方法]采用RT鄄PCR方法检测32例MDS患者及10例正常骨髓单个核细胞(bonemarrowmononuclearcell,BMMNC)中PTTGmRNA的表达。
短句来源
     Results After treatment with FITC-labeled G3139 at the concentration of 0 60 μmol·L -1 for 4 h, the G3139 uptake into peripheral blood mononuclear cell and bone marrow mononuclear cell in hematological tumor patients was significantly higher than that in normal control.
     结果 经过 0 60 μmol·L-1 荧光素FITC标记的G31 39作用 4h后 ,血液系统肿瘤患者外周血及骨髓单个核细胞对G31 39的摄取能力明显高于正常人 ;
短句来源
     Methods Expression of MPO was detected in bone marrow mononuclear cell(BMMNC) from 36 cases with ANLL and 30 normal controls by capillary electrophoresis (CE).
     方法采用毛细管电泳(CE)电化学检测法检测36例ANLL患者及30例正常骨髓单个核细胞(BMMNC)中MPO表达。
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  “bone marrow mononuclear cell”译为未确定词的双语例句
     We measured serum levels of soluble Fas(sFas) in 68 patients with hematological malignancy by using sandwich ELISA and detected bone marrow mononuclear cell membrane Fas(mFas) positive cells in 46 patients with acute leukemias by immunohistochemical technic.
     用ELISA法测定68例恶性血液病患者血清可溶性48kD跨膜糖蛋白(sFas)水平,用免疫组织化学SABC法检测其中46例急性白血病患者骨髓单个核细胞膜Fas(mFas)阳性表达细胞数。
短句来源
     Effect of GPI-PLD on Adhesion Function of Bone Marrow Mononuclear Cell from Patients with Myeloid Leukemia and Its Mechanism
     糖基化磷脂酰肌醇特异性磷脂酶D(GPI-PLD)对髓性白血病骨髓细胞黏附功能的影响及机制探讨
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     Sustained effect of autologous bone marrow mononuclear cell injection in patients with refractory angina pectoris and chronic myocardial ischemia: Twelve-month follow-up results
     自体骨髓单核细胞注射对顽固性心绞痛伴慢性心肌缺血患者的持久作用:12个月的随访结果
短句来源
     In order to explore the mechanism of differences in survival ability of CD59 and CD59 bone marrow mononuclear cell (BMMNCs) among the normal controls and PNH groups the expression of Bcl-2 and Bax proteins on both normal (CD59+ ) and abnormal (CD59- ) BMMNCs in patients with PNH was observed before and after liquid culture and was compared with normal controls.
     观察了阵发性睡眠性血红蛋白尿症(PNH)病人骨髓中正常及异常造血细胞培养前、后Bcl-2及Bax的表达,并与正常对照作了比较,以探讨二者生存能力差异的可能机制。
短句来源
     On day 7, 14, 21 and 28 after BMT, the femora were taken and the bone marrow mononuclear cell (BMMNC) suspensions were used for the cultivation of bone marrow stromal cells according to Dexter's culture method. The mRNA and protein expressions of bFGF in BMSC were assayed by RT-PCR and Western blot respectively.
     在BMT后第7、14、21、28天处死小鼠,用RT-PCR和Westernblot方法检测骨髓基质细胞bFGFmRNA及其蛋白表达水平。
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  bone marrow mononuclear cell
Alterations in human circulating and bone marrow mononuclear cell polyamine levels in hematologic malignancies as a consequence
      
Interleukin-1 and interleukin-6 production in peripheral blood and bone marrow mononuclear cell culture in multiple myeloma
      
The methods of therapeutic angiogenesis include endothelial progenitor cell (EPC) mobilization with cytokines [e.g., granulocyte colony-stimulating factor (G-CSF)] and bone marrow mononuclear cell (BMMNC) transplantation.
      
Additive effect of endothelial progenitor cell mobilization and bone marrow mononuclear cell transplantation on angiogenesis in
      
Treatment of HL60 cells with 1 μg/ml HuM195-gelonin resulted in more than 1000 times lower colony formation; normal bone marrow mononuclear cell colonyforming units treated with HuM195-IT were reduced by a factor of 10.
      
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We established a semisolid colony culture assay for acute lymphoblastic leukemic cells in vitro. The basic principle of this technique is identical to that used successfully for normal progenitors and acute myeloblastic leukemic cells except that this technique requires feeder cells and low O_2 tension (5%). Bone marrow mononuclear cells depleted of T cells were cultured with media conditioned by T cells in the presence of phytohemagglutinin and irradiated nonadherent...

We established a semisolid colony culture assay for acute lymphoblastic leukemic cells in vitro. The basic principle of this technique is identical to that used successfully for normal progenitors and acute myeloblastic leukemic cells except that this technique requires feeder cells and low O_2 tension (5%). Bone marrow mononuclear cells depleted of T cells were cultured with media conditioned by T cells in the presence of phytohemagglutinin and irradiated nonadherent mononuclear cells, Cells in the colonies had a characteristic pattern, with same cytochemical staining and abnormal karyotype as the original leukemic marrow. The results suggest that the secondary plating efficiency as tested in recloning studies of the primary blast colonies is predictive of the clinical outcome. The assay appears to help detection of small number residual leukemic cells according to the colony pattern. We consider this technique will be valuable in predicting the sensitivity of chemotherapy and in evaluating the quality of purged marrow for autologous marrow transplantation.

本文采用PHA-TCM及饲养细胞作为刺激条件,并在低氧环境下,建立了急淋L-CFU体外半固体培养方法。22例急性淋巴细胞白血病和恶性淋巴瘤患儿骨髓细胞培养,成功率为91%。集落数与接种细胞数呈线性关系。通过事先去除成熟T淋巴细胞。并根据集落细胞形态、细胞化学染色、染色体及集落细胞传代培养等检查,证明集落细胞属急淋细胞性质。本组试验提示第二代集落得率与临床预后可能有一定关系,同时通过集落培养可协助临床鉴别诊断,并可检测骨髓细胞中白血病细胞残存,为开展自身骨髓移植和体外药敏试验提供一个临床试验的手段。

T cell clones from human PBL of healthy adults treated by recombinant interleukin-2 (rIL-2) showed inhibitory effect on myeloid progenitor cells growth when the cloned T cells were added into a semisolid medium with a shorter pre-incubation (0.5-4 hours) with bone marrow mononuclear cells (BMMNC).The degree of colony inhibition for GM was well related to the time of T-BMMNC interaction as well as to the number of cloned T cells added into cultures.No substantial difference...

T cell clones from human PBL of healthy adults treated by recombinant interleukin-2 (rIL-2) showed inhibitory effect on myeloid progenitor cells growth when the cloned T cells were added into a semisolid medium with a shorter pre-incubation (0.5-4 hours) with bone marrow mononuclear cells (BMMNC).The degree of colony inhibition for GM was well related to the time of T-BMMNC interaction as well as to the number of cloned T cells added into cultures.No substantial difference of the inhibitory effect was found between autologous and allogenic T cell clones under same condition.These cloned T cells,with con- tinued rIL-2-stimulation,also showed either good ability of expansion in vitro or lymphokine- activated killer (LAK) activity against NK-sensitive erythroleukemic cell line K562 and NK- resistant human liver cancer cell line H7402 in a 4 hours ~(51)Cr release assay.Phenotypic analysis by flow cytometry indicated that these T clone cells carried T_3,T_8.Tac and DR antigens.These data suggest that cell-to-cell interaction can be the main way at least in the inhibitory effect of rIL-2-activated T clone cells on growth of myeloid progenitor cells,that may be involving cytotoxicity as well as LAK activity and contact inhibition.

人外周血 T 克隆细胞在 YIL-2持续刺激下具有良好的体外扩增能力和LAK 活性。在半固体琼脂培养体系中,T 克隆细胞抑制骨髓 CFU-GM 形成,抑制效应与 T 细胞数量、T 细胞和骨髓细胞的预孵育时间呈正相关。自体及异体 T 克隆细胞对 CFU-GM 的抑制作用无显著差异。经 FACS 表型分析,这种 IL-2激活的 T 克隆细胞为T_3(+)、T_4(-)、T_8(+)、DR(+)、Tac(+)。

A CFU-GM-inhibiting activity was found in supernatants of aplastic anemia patients' PHA-induced peripheral blood mononuclear cells and bone marrow mononuclear cells.It was strongly correlated with the γ-IFN concentration and could be eliminated by anti-γ- IFN McAb.With presence of cyclosporin A,the γ-IFN produced by peripheral blood mo- nonuclear cells of the patients studied reduced significantly,followed by a marked decrease in CFU-GM-inhibiting activity in the supernatants...

A CFU-GM-inhibiting activity was found in supernatants of aplastic anemia patients' PHA-induced peripheral blood mononuclear cells and bone marrow mononuclear cells.It was strongly correlated with the γ-IFN concentration and could be eliminated by anti-γ- IFN McAb.With presence of cyclosporin A,the γ-IFN produced by peripheral blood mo- nonuclear cells of the patients studied reduced significantly,followed by a marked decrease in CFU-GM-inhibiting activity in the supernatants just as caused by anti-γ-IFN McAb. All above indicate that cyclosporin A can induce the release of hematopoietic progenitor from γ-IFN-mediated suppression,which may be one of the mechanisms of cyclosporin A in tre- atment of aplastic anemia.

植物血凝素(PHA)可诱导再生障碍性贫血(AA)病人外周血及骨髓单个核细胞产生 CFU-GM 抑制活性,此活性与上清液中γ-干扰素(IFN-γ)的含量高度相关,并可被γ-干扰素单克隆抗体所中和。加入环孢菌素 A(CsA)(1μg/ml)能有效地抑制 IFN-γ的产生,从而消除其对 CFU-GM 的抑制,提示上述作用可能为 CsA 治疗 AA 的机理之一。

 
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