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dna transfer     
相关语句
  dna转移
     Construction of DNA Transfer System of Streptomyces tenebrarius
     黑暗链霉菌DNA转移系统的建立
短句来源
     DNA transfer was studied using pollen tube passage method with wild rice(Zizania) as donor and rice (O. Sativa) as recipient.
     本试验选用菰(Zizania)作供体,栽培水稻品种为受体,采用花粉管通道法进行DNA转移的研究。
短句来源
     The genotypes and the state of a suitable starting material were shown to be important in the competence for T DNA transfer.
     小麦基因型及转化材料的起始生理状态是影响TDNA转移的重要因素。
短句来源
     From 1990s,the experience in in vitro culture has been fused successfully into the techniques of DNA transfer.
     90年代以来 ,离体培养方面的经验被有效地融入DNA转移技术 ;
短句来源
     The role of proteins encoded by related genes determined in the Ti plasmid virulence region ( vir genes), in the bacterial chromosome, as well as in the plant chromosome in the process of T DNA transfer were introduced.
     植物遗传转化技术近年在农作物性状改良、植物生物反应器利用以及基因功能鉴定等方面得到了广泛的应用 . T -DNA转移是植物细胞农杆菌介导遗传转化整合和表达外源基因的基础 .
短句来源
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  dna转染
     Experimental Study on Sperm-mediated Foreign DNA Transfer
     外源DNA转染精子的实验研究
短句来源
  dna转化
     200 citrinin mutants were screened with the inhibition zone method from the transformants library of Monascus ruber M-7 by Agrobacterium-mediate DNA transfer, which contains more than 5,000 transformants. Then 53 mutants, whose citrinin contents ranged from 0.04μg/g to 154.57μg/g in the red fermented rice (RFR), were achieved by high performance liquid chromatography (HPLC). Color values of RFR prepared by these mutants were also detected.
     以农杆菌介导法建立的红曲霉T-DNA转化库为实验材料,采用抑菌圈法从5,000多个转化子中筛选出200株桔霉素突变子的候选菌株,用高效液相色谱法进一步筛选得到53株与出发菌株相比产桔霉素能力发生显著变化的突变子,其红曲中桔霉素含量介于0·04~154·57μg/g之间。
短句来源
  dna转运
     Results Artificial synthetic DNA transfer system and modified viral vectors could efficiently transfect target cells and get high level expression.
     结果 合成的DNA转运系统和经修饰的病毒载体体外转染靶细胞后 ,可获得显著表达。
短句来源

 

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      dna transfer
    The efficiency of DNA transfer into the cells also depends on DNA/poly(L-Lys)Gal molar ratio in the complexes.
          
    A new method of plasmid DNA transfer from the donor strainEscherichia coliS17-1 to the erythromycin-producing strainSaccharopolyspora erythraeaand avermectin-producing strain Streptomyces avermitilis via intergeneric conjugation was proposed.
          
    The possible mechanism of alien DNA transfer was discussed.
          
    Many details of the key molecular events within the bacterial cells involved in T-DNA transfer have been elucidated, and it is notable that some plant factors which were elusive before are purified and characterized.
          
    Cortical reflex myoclonus in patients with the mitochondrial DNA transfer RNALys(8344) (MERRF) mutation
          
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    The Ti T-DNA (Transferred DNA from tumor-inducing plasmid) transformed tissues of sunflower have been obtained by invitro infecting cotyledons and hypocotyls with strains B6S3 or T37 of Agrobacterium tumefaciens. Following subculture on hormone-free medium for nearly one year, the Ti T-DNA transformed tissue lines were utilized to carry out protoplast culture and cell culture. The culture medium suitable for protoplast culture of B6S3-and T37-transformed lines were respectively C81V (Dutitz...

    The Ti T-DNA (Transferred DNA from tumor-inducing plasmid) transformed tissues of sunflower have been obtained by invitro infecting cotyledons and hypocotyls with strains B6S3 or T37 of Agrobacterium tumefaciens. Following subculture on hormone-free medium for nearly one year, the Ti T-DNA transformed tissue lines were utilized to carry out protoplast culture and cell culture. The culture medium suitable for protoplast culture of B6S3-and T37-transformed lines were respectively C81V (Dutitz et al., 1977) and DPD (Durand et al., 1973) medium, in which diffenent hormones and carbohydrates were supplemented. After culture in thin-liquid layer of culture medium for 3-5 days, the cultured protoplastsunderwent sustained divisions, and for 10 days, the protoplast-derived colonies were formed. Interestingly, the B6S3-transformed lines could also give rise to proembryo-similar structure directly from protoplast-regenerated cells. The cell colonies from cell culture of transfomed lines were still retained the traits of both phytohormone autotrophy and opine synthetase activity, which are encoded for by Ti T-DNA.

    根癌农杆菌离体感染向日葵子叶、下胚轴外植体形成的Ti T-DNA转化组织在激素条件下长期继代培养后,用来进行原生质体培养和细胞培养。适于B6S3转化系和T37转化系原生质体培养的培养基分别为附加不同激素和糖类的C81V和DPD培养基。用液体浅层法培养3~5天时,原生质体开始分裂。10天后形成细胞团。B6S3转化系还可直接从原生质体产生原胚状结构。转化系的细胞克隆均保持着激素自主型生长特性和冠瘿碱合成酶合成特性。

    The introduction and expression of TiT-DNA(transferred DNA on Ti plasmid)genes, harbored by strains B6S3 or T37 of Agrobacterium tumefaciens, into sunflower cells not only resulted in transformed cells capable of synthesizing special gene products but also enabled them to utilize D-lactose as a sole carbon source and be resisrant to some metabolic analogtles(e.g. BUdR). Moreover, a number of physiological and biochemical activites in host plan cells could also be affected by integrated TiT-DNA...

    The introduction and expression of TiT-DNA(transferred DNA on Ti plasmid)genes, harbored by strains B6S3 or T37 of Agrobacterium tumefaciens, into sunflower cells not only resulted in transformed cells capable of synthesizing special gene products but also enabled them to utilize D-lactose as a sole carbon source and be resisrant to some metabolic analogtles(e.g. BUdR). Moreover, a number of physiological and biochemical activites in host plan cells could also be affected by integrated TiT-DNA genes. The peroxidase activity, isoperoxidase gene expression, protein composition and proportion, and free amino-acid pool were significantly different between transformed lines(both B6S3 lines and T37 lines)and normal lines of sunflower which growed on either hormone-containing media or hormorte-free media. The precursor aminoacids related to opine biosynthesis had a higher metabolic levels in these transformed lines.

    根癌农杆菌B6S3和T37菌株Ti质粒T-DNA基因(TiT-DNA)在向日葵基因组中的导入与表达,不仅使转化细胞产生特殊的基因产物,并且使之能将D-乳糖作为唯一碳源利用和对某些代谢类似物(BUdR)具有抗性,此外还影响宿主细胞一系列生理生化活动。在无激素和含激素两种培养条件下,向日葵TiT-DNA转化系与相应正常系在过氧化物酶的活性及其同工酶基因的表达、蛋白质的种类及比例、游离氨基酸库等均存在着较大的差异。在转化系内,有关冠瘿碱生物合成的前休氨基酸具有较高的代谢水平。

    The mesophyll protoplasts of Phaseolus vulgaris L.were used as the receptor and NPT-Ⅱ as the exogenous gene in an electroporation study.After electroporation,the protoplasts were cultured on BSB Liquid Medium.About one month later,calli from the treated protoplasts were observed.When transferred onto the selective solid MS medium containing 50μg/lm kanamycin(Km),3mg/12,4-D and 0.2mg/16BA,some calli grew rapidly and showed obvious resistance to kanamycin,indicating that electroporation-mediated DNA transfer...

    The mesophyll protoplasts of Phaseolus vulgaris L.were used as the receptor and NPT-Ⅱ as the exogenous gene in an electroporation study.After electroporation,the protoplasts were cultured on BSB Liquid Medium.About one month later,calli from the treated protoplasts were observed.When transferred onto the selective solid MS medium containing 50μg/lm kanamycin(Km),3mg/12,4-D and 0.2mg/16BA,some calli grew rapidly and showed obvious resistance to kanamycin,indicating that electroporation-mediated DNA transfer of chimeric genes encoding neomycin phospho—transferase resulted in stably transformed calli of P.vulgaris that were resistant to kanamycin.The effects of various voltage,pulse length and a few other factors influencing the viability and gene transfer efficiency of protoplasts were also measurcd.As the voltage of pulse length increased,the viability of protoplasts(but not the transfer)was reduced. Fourteen kanamycin-resistant(km~r)calli were obtained from 826 calli on the selective medium,including the treatments of 160V~100mS,160V~65mS,200V~100mS, 200V~60mS,130V~130mS and 100V~100mS.The efficiency of transfet was 1.5%.

    以菜豆叶肉细胞原生质体为受体研究了以NPT-Ⅱ为外源基因的电脉冲转化方法,并对脉冲电压、宽度等进行了测定。从862块电脉冲处理的原生质体愈伤组织中,筛选出具有Km~γ的转化愈伤组织14块。转化率为1.5%。

     
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