The original generation cells were smaller than the next generations which appeared polygonal, fusiform or irregular with black granule in the cytoplasm.
The results showed that, after being incubated with a fragment of P195 (M6, amino acids from 384 to 595) and put into silver developing solution, the erythrocyte was found to have black granule silver on its surface.
This increased the reflectance of the black granule to 0.16.
Silver stained nucleolar granules were detected in the esophageal cancer cells(ECa 109)in vitro,while they were absent in primary culture of normal esophageal epithelial cells.Immediately after hyperthermic treatment the nucleoli were obscured and the nucleolar granules also disappeared.These granules recovered gradually as the cells were returned to normal culture condition.Analysis of the mitotic stages indicated that the silver-stained structure still persisted at prophase,but hardly seen...
Silver stained nucleolar granules were detected in the esophageal cancer cells(ECa 109)in vitro,while they were absent in primary culture of normal esophageal epithelial cells.Immediately after hyperthermic treatment the nucleoli were obscured and the nucleolar granules also disappeared.These granules recovered gradually as the cells were returned to normal culture condition.Analysis of the mitotic stages indicated that the silver-stained structure still persisted at prophase,but hardly seen at metaphase and anaphase,and during telophase a lot of scattered black granules existed over the whole nuclear region.Cases of nucleoli expelled from nuclei to cytoplasm were also seen in these cells.The occurreuce of nucleolar extrusion in cancer cells varied from O to more than(10%).Zinc sulfate increased the rate of nucleolar extrusion,but the effect of hyperthermia was not significant.The relation of nucleolar extrusion and cellular me- tabolism was discussed.
Eight pigs which are infested separately with Patagonimus wester-mani metacercaria, excysted worms and adult worms of Parag-onimus, are controlled with dogs. Autopsies are performed at different intervals, varying from 16-107 days, and the chief results include. ( 1 )When pigs are infested with metacercaria or excysted worms, the worms migrating from the muscles are slightly bigger than those of metacercaria. Black granules are usually seen in excretory bladder and a single small stylet may be occasionally...
Eight pigs which are infested separately with Patagonimus wester-mani metacercaria, excysted worms and adult worms of Parag-onimus, are controlled with dogs. Autopsies are performed at different intervals, varying from 16-107 days, and the chief results include. ( 1 )When pigs are infested with metacercaria or excysted worms, the worms migrating from the muscles are slightly bigger than those of metacercaria. Black granules are usually seen in excretory bladder and a single small stylet may be occasionally present in some of them. (2) Paragonimus westermari adult worms with which pigs are inoculated separately into the subcutaneous tissues are the peritoneal cavities are atrophied or dead. (3)When the adult worms are inoculated into the pleural cavities, some of them are found settling down in the lungs and forming cysts there 45 days later. Based on this study, the pig may be considered the paratenic host rather than the final host of Paragonimus westermani.
Uroleptoides qingdaoensis is a new hypotrichous ciliate from soil in Qingdao, China. It shows the characteristics of genus Uroleptoides (Hemberger, 1982).Uroleptoides qingdaoensis sp. nov. (Figs. 1-7), body elongated, dorsoventral flattened and measures in vivo 150-200 μm. The cytoplasma is colorless, often with black granules. It feeds on bacterias, flagellates, diatoms and small ciliates. The macronucleus is placed in the left part of the cell and consists of 10 to 16 fragments. The infraciliature of...
Uroleptoides qingdaoensis is a new hypotrichous ciliate from soil in Qingdao, China. It shows the characteristics of genus Uroleptoides (Hemberger, 1982).Uroleptoides qingdaoensis sp. nov. (Figs. 1-7), body elongated, dorsoventral flattened and measures in vivo 150-200 μm. The cytoplasma is colorless, often with black granules. It feeds on bacterias, flagellates, diatoms and small ciliates. The macronucleus is placed in the left part of the cell and consists of 10 to 16 fragments. The infraciliature of Urole-ptoides qingdaoensis differs in the following features from the other species of the genus: the dorsal side bears a fronto-terminal row of 2 to 3 cirri, probably an anterior fragment of the right marginal cirri. Three transvers cirri are arranged in the longitudinal axis of the body and 1-2 postoral cirri are present.The species was cultured by the method of Buitkamp and Wilbert (1974).For revealing the infraciliature the protargol silver staining method according to Wilbert (1975) was used.Type location: Soil of the hill Baguan, Qingdao (35°08'N, 120°43'E), Shandong China.Type-specimens: Slides of holotype specimens have been deposited in Fishery College, Ocean University of Qingdao.
black granule
黑色颗粒
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