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restriction analysis
相关语句
  酶切分析
     Restriction analysis showed that the characterization of the clone of 1.6 kbp, 2.2 kbp and 2.5 kbp was different.
     限制性内切酶酶切分析表明,2.2kbp,2.5kbp和1.6kbp的克隆具有不同的特征。
短句来源
     Results The recombinant plasmid containing hIL15M—TNFαM fusion gene was obtained as demonstrated by restriction analysis.
     结果 经酶切分析 ,所构建的质粒均插入hIL15M -TNFαM融合基因。
短句来源
     Methods: The GST M1 TV2 cDNA was amplified from human lung total RNAs by RT-PCR and was recombined with eukaryotic expression vector pcDNA3.1.The recombined plasmid pcDNA3.1-GST M1 TV2 was verified with PCR,restriction analysis, and sequencing determination.
     方法 :用RT PCR技术从人肺脏组织总RNA中分离扩增人GSTM1TV2基因的cDNA序列 ,将其插入到真核表达载体pcDNA3.1多克隆位点中 ,构建真核重组表达质粒pcDNA3.1 GSTM1TV2 ,并用PCR扩增、酶切分析及序列测定等方法对重组质粒进行鉴定。
短句来源
     coli-DH5α and screened with colony PCR and restriction analysis.
     coli-DH5α,菌落PCR、酶切分析和DNA序列分析鉴定重组克隆。
短句来源
     Results Restriction analysis of plasmid, drug susceptibility testing and DNA fingerprinting of chromosome DNA of JM83/16SN indicated that the competitive template had been inserted in chromosome of JM83/16SN.
     结果 细菌质粒限制性酶切分析、药敏试验及染色体DNA指纹图谱分析证实竞争模板已插入到JM83/16SN染色体中。
短句来源
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  限制酶切分析
     Restriction analysis indicated that pHT 1Aa, pHT 1Ca and pHT 2Ab had the typical physical map of the homologous cry genes.
     限制酶切分析表明 ,含有cry1Aa、cry1Ca和cry2Ab基因的克隆片段均含有相应基因的保守物理图谱。
短句来源
     Restriction analysis indicated that all eight recombinants had inserts whose sizes ranged from 0. 6 to 1. 7kb.
     限制酶切分析表明它们均含有插入片段,大小为0.6-1.7kb。
短句来源
     Two of them were further characterized by restriction analysis and DNA sequencing.
     其中两个克隆并用限制酶切分析及DNA序列测定做进一步鉴定。
短句来源
     Direct sequencing of PCR-amplified fragments revealed a recurrent missense mutation, R190W (568C>T), in RUNX 2. The mutation was further con firmed by Hae III restriction analysis.
     PCR扩增片段直接测序显示患儿RUNX 2外显子 2内发生R190W (5 6 8C >T)错义突变。 该突变通过PCR产物的HaeIII限制酶切分析得到进一步确认。
短句来源
     The results of restriction analysis and sequencing analysis confirmed that the sequence of the0. 3kb inserted fragment were exactly the same sa K562mRNA junction region.
     限制酶切分析和DNA序列分析结果都证实插入片段序列与K562mRNA接合区完全一致。
短句来源
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  “restriction analysis”译为未确定词的双语例句
     Method Primer-introduced restriction analysis PCR (PIRA-PCR), tetra-primers amplification refractory mutation system (ARMS) and single strand conformational polymorphism (SSCP) were applied to detect 3 single nuclear polymorphysm (SNP) loci in bcl-2 gene (dbSNP: rs1800477, rs1801018, rs1564483), 3 SNP loci (dbSNP: rs6060900, rs6089046, rs5841091) in bcl-x gene and a microsatellite in bax gene.
     方法采用引物引入限制性内切酶分析(PIRA-PCR)、四引物扩增阻滞突变系统以及单链构象多态性(SSCP)技术,分析100名正常人和88例SLE患者bcl-2基因的3个单核苷酸多态性(SNP)位点(dbSNP:rs1800477,rs1801018,rs1564483)、bcl-x基因的3个SNP位点(dbSNP:rs6060900,rs6089046,rs5841091)及bax基因的1个微卫星位点。
短句来源
     Clone the amplified HSP70 and MAGE-4 epitope genes into pGEM-T easy vector and,after identification of the constructed recombinant plasmid by restriction analysis,subclone HSP70 gene into prokaryotic expression vector pET28a,and MAGE-4 gene downstream to HSP70.Transformed the constructed recombinant plasmid pET28a-HSP70-MAGE-4 to E.
     将二者分别克隆入pGEM-Teasy载体,酶切鉴定后,将HSP70基因亚克隆入pET28a原核表达载体,再将MAGE-4基因克隆入HSP70基因的下游,构建重组质粒pET28a-HSP70-MAGE-4。
短句来源
     Results: MIP-2γ/ GM-CSF fusion gene vector was confirmed by restriction analysis and sequencing analysis.
     结果:MIP-2γ/GM—CSF融合基因载体构建正确;
短句来源
     Two S1 genes were cloned into plasmid pBluescript Ⅱ ks+ by ligation of cohesive end. The two recombinant plasmids of ARVS1133S1-pBluescript and ARV1733S1-pBluescript were identefied by PCR and restriction analysis.
     将扩增到的这两个毒株的S1基因通过粘端连接分别克隆到pBluescript ⅡKS+质粒中,用PCR和限制性内切酶分析鉴定,表明获得二种重组质粒ARVS1133S1-pBluescript、ARV1733S1-pBluescript。
     The plasmid pMD-18T-ap33 and pcDNA3.1(+) were digested by BamHⅠand XbaⅠ. The ap33 gene was subcloned into the plasmid pcDNA3.1(+). The recombinant plasmid pcDNA3.1(+)-ap33 was identified by PCR and restriction analysis.
     鉴定出的重组质粒pMD-18T-ap33和pcDNA3.1(+)空质粒经BamHⅠ和XbaⅠ限制性内切酶双酶切,凝胶电泳后回收ap33目的基因和pcDNA3.1(+)空质粒酶切片段,将ap33基因亚克隆入pcDNA3.1(+)载体并进行筛选和鉴定。
短句来源
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  restriction analysis
Restriction analysis of DNA coding for 16S rRNA showed that both strains can be assigned to Bacillus licheniformis.
      
The eleven most active oil-degrading and PAH-degrading strains were genotyped by a polymerase chain reaction with BoxA1R primers and a restriction analysis of ribosomal DNA amplicons.
      
The method is based on multiplex polymerase chain reaction (MPCR) with species-specific primers and does not require nucleotide sequencing or restriction analysis of PCR products.
      
Temperate Bacteriophage ZF40 of Erwinia carotovora: Phage Particle Structure and DNA Restriction Analysis
      
The ZF40 phage genome is 45.8 kb in size, as determined by restriction analysis, and contains DNA cohesive ends.
      
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The 2.17 kb EcoRI/BamHI restriction fragment of the mtDNA from Beijing duck liver was cloned, using plasmid pAT153 as a vector and E. coli SK1592 as recipient cells. The recombinants have been identified by restriction analysis and Southern blot analysis. Moreover, the stability of the chimeric plasmids in recipient cells has been studied and the question of nonsuccessful cloning of another EcoRI/BamHI fragment (2.81 kb) of duck liver mtDNA is also discussed.

本文以质粒pAT153为载体,以E.coli SK1592为受体菌,将北京鸭肝脏mtDNA的2.17kb的EcoRI/BamHI限制片段进行了克隆,并对重组质粒作了限制性内切酶分析、Southern吸印与杂交分析。此外,还对重组体的一些性质作了初步研究,并对鸭肝mtDNA的另一个EeoRI/BamHI片段(2.81kb)不能被克隆的原因进行了讨论。

SalI restriction fragments of the spinach chlorplast DNA were cloned into the Sail site of pBR322 DNA. Altogether 52 recombinant transferments (Ap'Tc") were selected and each of them was screened by restriction analysis. It was proved by Southern blot and probe hybridization that the recombinant plasmid pSS104 contains the 4.1 kb SalI fragment of the spinach chloroplast genes have been mapped, on this fragment. The transcription products of the cloned fragment have not been discovered with E. coli in vivo...

SalI restriction fragments of the spinach chlorplast DNA were cloned into the Sail site of pBR322 DNA. Altogether 52 recombinant transferments (Ap'Tc") were selected and each of them was screened by restriction analysis. It was proved by Southern blot and probe hybridization that the recombinant plasmid pSS104 contains the 4.1 kb SalI fragment of the spinach chloroplast genes have been mapped, on this fragment. The transcription products of the cloned fragment have not been discovered with E. coli in vivo system.

利用pBR 322衍生质粒DNA为载体,将菠菜叶绿体DNA的SalⅠ限制性片段插入质粒的Sal Ⅰ位点,获得52个含重组质粒的菌落(Ap~rTc~5)。并对每个克隆的质粒进行限制性内切酶分析,通过Southern吸印与探针杂交,证明了重组质粒pSS104含有的插入DNA是菠菜叶绿体DNA 4.1kb的SalⅠ片段。迄今在该片段尚未定位任何已知的叶绿体基因,用大肠杆菌的活体系统也未能发现这段DNA的转录产物,本文对此进行了讨论。

In this investigation, 17 mtDNAs of Chinese Han people were used. By restriction analysis, cleavage fragments of Hinc Ⅱ, BamHI and Pvu Ⅱ showed RFLP. In assessment of hereditary similarities among mtDNAs, the mean value of nucleotide diversity obtained was 0.0064. This is reasonably agreeable with 0.0036 (Brown, 1980), 0.004 (Cann, 1982) and 0.00417 (Horai, 1984), but is quite different from 0.02 obtained by Aquado (1983) who analysed the 900 bp of D-loop in the mtDNAs of 7 people. In the study of mutation...

In this investigation, 17 mtDNAs of Chinese Han people were used. By restriction analysis, cleavage fragments of Hinc Ⅱ, BamHI and Pvu Ⅱ showed RFLP. In assessment of hereditary similarities among mtDNAs, the mean value of nucleotide diversity obtained was 0.0064. This is reasonably agreeable with 0.0036 (Brown, 1980), 0.004 (Cann, 1982) and 0.00417 (Horai, 1984), but is quite different from 0.02 obtained by Aquado (1983) who analysed the 900 bp of D-loop in the mtDNAs of 7 people. In the study of mutation mechanism, it was found in 6 cases that there were gains and losses of sites due to nucleo- tide substitution. 3 eases of restriction site gains caused by point mutation were discussed,

实验研究对象为17例中国汉族人的mtDNA。通过限制性分析发现3种限制酶(HincⅡ、BamHI和PvuⅡ)酶解片段有RFLP现象,在估计mtDNA之间遗传相似性时,所得到的核苷酸歧异频率的平均值为0.0064。这个值与Brown的0.0036、Cann的0.004和Hotrai的0.00417的值接近,而与Aquado对7个人mtDNA D-环区900个碱基对分析得到的0.02相差较大。在研究突变机制时,发现有6例由于核苷酸的替换引起了限制性位点的增减。文中对其中因点突变引起限制性位点增加的3例进行了讨论。

 
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