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toxin a
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  毒素a
     Construction and expression of toxoplasma major surface antigen P30 fusion protein with cholera toxin A_2/B in Bacillus coli
     用含霍乱毒素A_2/B的载体在大肠杆菌中表达弓形虫主要表面抗原P30
短句来源
     Methods Obtained P30 gene fragment by PCR and inserted it to plasmid pUAB024 containing cholera toxin A 2/B subunit gene,Expressed fusion protein in JM109(DE3) and detected by running SDS-PAGE and Western blotting.
     方法 应用PCR方法扩增出P30基因片段后 ,克隆入含有霍乱毒素A2 /B亚基基因的表达质粒pUAB0 2 4 ,在大肠杆菌JM10 9(DE3)中表达融合蛋白。
短句来源
     Aim To Constructed prokaryotic expressing plasmid pCT-P30 containing Toxoplasma gondii major surface antigen P30 and cholera toxin A 2B subunit gene and express P30-CTA 2/B fusion protein.
     目的 克隆并表达含有弓形虫P30抗原及霍乱毒素A2 /B亚基基因的原核表达载体 ,为弓形虫疫苗的研究奠定基础。
短句来源
     Gene cloning and high expression of clostridium difficile toxin A C-terminal repeated unit
     艰难梭菌毒素A羧基端基因的克隆与表达
短句来源
     Objective To discuss the long term curative effect of botulinum toxin A(BTXA) for treating spasm of eyelid and facial muscle.
     目的 探讨肉毒杆菌毒素 A(botulinum A toxin,BTXA)治疗眼睑及面部肌肉痉挛的远期疗效。
短句来源
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  a毒素
     Comparative Study on Apoptosis Indu ction of SMMC7721 and Vero Cells by Clostridium difficile Toxin A
     艰难梭菌A毒素诱导SMMC7721细胞和Vero细胞凋亡的比较研究(英文)
短句来源
     Cell cycle distribution was analyzed by flow cytometr y. RESULTS :Toxin A (0.293-4.690mg ·L -1 )inhibited proliferation of SMMC7721and Vero cells in a time-and concentration-dependent manner.
     结果:不同浓度A毒素(0.293~4.690mg·L-1)明显抑制了SMMC7721及Vero细胞的增殖,并且呈时间和浓度依赖性;
短句来源
     difficile in faeces ranged from 10~4 to 10~8 CUFU/g,the average level of toxin in faecal extracts mainteined 10~3cu/g(cytotoxin)or 10~2/g(toxin A,RPHA titers) during 1 to 14 days of O.
     difficile攻击后的1~14天,小鼠粪便中C. difficile菌数在10~4至10~8CFU/g内变化,细胞毒素为10~3CFU/g,A毒素滴度为10~2/g,B.adolescentis也一度下降10~2CFU/g。
短句来源
     This paper described the cell killing activity of toxin A of Clostridium difficile on four cell lines which were Vero(Africa green monkey kidney),TPC-1 (human thyroid papillary carcinoma),NIH 3T3 (mouse fibroblast)and NIH 3T3 ras (NIH 3T3 cell transfected with ras oncogene)cell line.
     本文报道艰难梭菌A毒素对4种培养细胞的细胞致死活性的探讨。 4种培养细胞为Vero(非洲绿猴肾细胞)细胞、TPC─1(人甲状腺肿瘤细胞)细胞、NIH3T3细胞(小鼠成纤维细胞)及将ras癌基因转基因于NIH3T3细胞的NIH3T3ras细胞。
短句来源
     CONCLUSION The antitumor activity of toxin A on TPC 1 cells was much higher than that on vero cell line.
     结论 A毒素对TPC 1细胞的杀伤作用远大于对Vero细胞的作用。
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  “toxin a”译为未确定词的双语例句
     After follow up for 6~36 months, the recurrence rate of botulinum toxin A group and haloperidol group was 63.6%(14/22) and 95.0% (19/20) (χ 2=4.76, P<0.05).
     在 6~ 36个月的随访期间 ,A型肉毒毒素治疗组和氟哌啶醇对照组的复发率分别为 6 3 6 %(14 / 2 2 )与 95 0 % (19/ 2 0 ) (χ2 =4 76 ,P <0 0 5 )。
短句来源
     Express of toxoplasma major surface antigen P30 fusion protein with cholera toxin A2/B in bacillus coli
     融合蛋白弓形虫主要表面抗原P30-CTXA2/B在大肠杆菌中的表达
短句来源
     Botulinum toxin A in the treatment of dystonia:a clinical analysis of 409 cases
     A型肉毒毒素治疗肌张力障碍:409例疗效分析
短句来源
     A clinical study on the treatment of congenital nystagmus by using of botulinum toxin A
     A型肉毒杆菌毒素治疗先天性眼球震颤临床研究
短句来源
     Methods Twenty-five patients failed in the conservative therapy were divided into two groups. In group A(n=13), the patients received the affected piriformis injections with 3 ml of 0.25% bupivacaine containing 5 mg triamcinolone under the fluoroscopy,and in group B(n=12), the affected piriformis injections with 3 ml of 0.25% bupivacaine containing 5 mg triamcinolone and 100 U botulinium toxin A .
     方法25例保守治疗无效的梨状肌综合征患者随机分为两组,A组患者13例,梨状肌内注射0.25%布比卡因3ml加5mg曲安奈德,B组12例,梨状肌内注射0.25%布比卡因3ml加5mg曲安奈德和100U的A型肉毒毒素。
短句来源
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  toxin a
Mean doses per muscle averaged 320 mouse units (mu; range 160-1000 mu botulinum toxin A prepared by CAMR, Porton Down, UK).
      
Ten patients with spastic drop foot were treated by local injections of botulinum toxin A (botulinum toxin A haemagglutinin complex).
      
Three patients suffering from gustatory sweating following trauma to the preuricular region from a bullet wound or parotid gland surgery were treated by intracutaneous injection of botulinum toxin A.
      
Long-term treatment of cervical dystonia with botulinum toxin A: efficacy, safety, and antibody frequency
      
The effect of botulinum toxin A (BTX) was studied on 12 patients with idiopathic craniofacial hyperhidrosis.
      
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The distribution of Bungarus fasciatus venom collected in Guangtong Province,China and its purified toxins(cardiotoxin-like A and B)in mice has been studiedby using the~(125)I-labeled preparation.The~(125) I-labeled venom,toxin A orB at sublethal dosage was injected subcutaneously in mice,which were sacrificedat 1,2,4,8,16 or 24 hours after the injection and the radioactivities(counts/min/mg wet tissue weight)in their various tissues(or organs)were measured.The ex-perimental results obtained from...

The distribution of Bungarus fasciatus venom collected in Guangtong Province,China and its purified toxins(cardiotoxin-like A and B)in mice has been studiedby using the~(125)I-labeled preparation.The~(125) I-labeled venom,toxin A orB at sublethal dosage was injected subcutaneously in mice,which were sacrificedat 1,2,4,8,16 or 24 hours after the injection and the radioactivities(counts/min/mg wet tissue weight)in their various tissues(or organs)were measured.The ex-perimental results obtained from the three groups of animals showed that exceptfor the blood the radioactivities in all tissues reached or approached their maximumat 2 hours after injection.The elimination of toxin B from the animal body mightbe quicker than that of toxin A and the venom.If the radioactivities of the kidneyswere taken as 100 per cent,those of the lung,liver and spleen were about 3-5% andthose of the diaphragm,heart and skeletal muscle,about 1-2%.The lowest levelwas observed in the brain,which was less than 0.5%.These results were similar tothose of ~(125)I-labeled Bungarus multicinctus venom and bungarotoxins.No special accumulation was found in any particular tissue.The autora-diograph of the diaphragm also showed that there was no accumulation of thepurified toxins in the neuromuscular junction region.

将动物分成三组,分别皮下注射小于致死剂量的~(125)I标记金环蛇毒或二个类心脏毒素(毒素 A或 B),在注射后1、2、4、8、16和24小时测定了一些组织(或脏器)中的放射强度(脉冲数/min/mg 湿重组织)。结果表明,除血液放射强度在注射后1小时已达最高值外,其余均在注射后2小时达到或接近最高值。至于三者从动物体内的排除,则毒素 B 似较快,如它在各组织中的放射强度在注射后8小时已降到接近对照水平,而毒素 A 和粗毒一般都在注射后16小时方降到对照水平,甚至还稍高于对照水平。注射后2小时,三种被标记物质在各组织中的放射强度均以肾为最高。如以肾的放射强度为100%计算,则肺、肝和脾的为3.5%,心肌、膈肌和骨胳肌的为1—2%,脑的最低,仅为0.3—0.5%。这些结果和 Lee 等报道的有关银环蛇毒在体内分布的资料相似。未观察到粗毒或二种纯化毒素在某一种组织中有特殊的积聚,用放射自显影法也未能显示毒素 A 或 B 在神经肌肉接头有明显的集中。

A method has boon discussed for the preparation of ochratoxin A under general laboratory condition. The Aspergillus ochraceus strain LY8604, one of 46 isolates from grain, is inoculated on moist whole wheat flour and incubated at 25℃ for three weeks. The toxin is extract-ed from the moldy materials with CIICf 3-MeOH (5 : 1).The extract, after filtration, is 1 iquid - liquid partitioned with 0.5M NaHCO3 solution. The supernatant is aeidified with 1N HCl or 1N H2SO4 and then rc-extracied with fresh chlofotrn....

A method has boon discussed for the preparation of ochratoxin A under general laboratory condition. The Aspergillus ochraceus strain LY8604, one of 46 isolates from grain, is inoculated on moist whole wheat flour and incubated at 25℃ for three weeks. The toxin is extract-ed from the moldy materials with CIICf 3-MeOH (5 : 1).The extract, after filtration, is 1 iquid - liquid partitioned with 0.5M NaHCO3 solution. The supernatant is aeidified with 1N HCl or 1N H2SO4 and then rc-extracied with fresh chlofotrn. Poar this exiraet into a silira gel column clute with C6H6 -CH 3COOH(9 : 1) and collect the grcenish yellow fluo-rsceat eltiate under UV lighi. This cluate is coneentrated to a small volume, and spotied on the preparing TLC silica gel plates, developing with Toluenc-EtOAc-90% Formic acid(5 : 4 : l). Under UV light, remove the greenish yellow fluorescent zones from the plates and elute on a ehroma-tographic column with CHCI3. Tie eluate is evaporated on steam hath under N2 condition to dryaess, and crystallized from henzene. The pro-duct is colorless crystalline with m.p. 89-91℃. It is identified as ochra-toxin A by UV, IR, NMR, Ms spectra and other methods.

本文建立了一般实验室条件下棕曲霉毒素A大的制备方法.以从粮食上分离的棕曲霉菌Ly8604作为生产菌株,接种于灭菌的湿全麦粉上,于25℃培养三周.培养物以氯仿:甲醇(5:1,V/V)提取,用碳酸氢钠水溶液进行液液分配,水相酸化后再用氯仿萃取,经硅胶柱层析净化.用苯:乙酸(9:1)洗脱,紫外光灯下收集黄绿色荧光溜分,浓缩后进行磋胶薄层层析,用甲苯:乙酸乙脂:90甲 酸(5:4:1)展开,紫外光灯下刮取黄绿色荧光谱带,再在色谱柱上用氯仿洗脱后于苯中结晶.产品为无色针状晶体.熔点为89℃~91℃,荧光最大激发波长为340nm ,最大发射波长为470nm.UV、IR、NMR和MS谱图证明产品是棕曲霉毒索A.

The sensitivity of isolated root cap cells of corn to Hm-toxin (a pathoto-xin produced by Helminthosporium maydis) was not only much higher than thatof root growth when treated with cultural filtrates,but closely correlated withthe lesion size induced by H.maydis.There was no correlation between the in-hibiting effect on root growth and the lesion size,on leaves,therefore,it shouldnot be used in bioassay to evaluate the resistance of corn inbreds to southernleaf blight.The results also indicated that the...

The sensitivity of isolated root cap cells of corn to Hm-toxin (a pathoto-xin produced by Helminthosporium maydis) was not only much higher than thatof root growth when treated with cultural filtrates,but closely correlated withthe lesion size induced by H.maydis.There was no correlation between the in-hibiting effect on root growth and the lesion size,on leaves,therefore,it shouldnot be used in bioassay to evaluate the resistance of corn inbreds to southernleaf blight.The results also indicated that the extracts of infected leaves hadthe best effect among the different preparations of Hm-toxin It could representthe actual situation of conidial inoculation on laeves,the coefficient of correla-tion is 0.9315.Both the crude purified Hm-toxin and the filtrates of cultureswere less effective.The experimental results also found ammonium tartrale am-ong components of the modified Fries medium could affect both bioassay andproduction of Hm-toxin,therefore,it was not satisfactory if the cultural filtra-tes were used to assay quantitatively Hm-toxin.

在试验的两种Hm-毒素生物测定法中,根冠细胞测定法的效果比种子根伸长法好,主要是前者对Hm-毒素的敏感性强,与病斑大小有极显著的相关性。抑制种子根伸长法由于不能确切反映玉米对小斑病的反应,因而不宜用作Hm-毒素的生物测定。在不同的毒素制剂中,以病叶提取液处理根冠细胞最能反映小斑菌接种玉米后的实际情况,与病斑大小的相关系数r=0.9315,居供试的三种制剂之首,其次是粗提制剂和培养物滤液。试验还发现酒石酸铵不仅影响Hm-毒素测定的效果,而且还影响毒素产生的量,因而进行Hm-毒素的生物测定,最好使用病叶提取液或用无酒石酸铵的改良Fries培养基制备的毒素粗提制剂或培养物滤液。

 
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