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chimeric toxin
相关语句
  嵌合毒素
     Effects of Recombinant Chimeric Toxin Dsg3EC_(1-2)PE40 on T and B Lymphocytes from Pemphigus Vulgaris
     重组嵌合毒素Dsg3EC_(1-2)PE40对寻常型天疱疮患者T、B淋巴细胞的影响
短句来源
     Expression and purification of recombinant Dsg3EC_(1-2)and the chimeric toxin molecule in prokaryotic cells
     重组Dsg3EC_(1-2)及其嵌合毒素蛋白的原核表达及纯化
短句来源
     Cytotoxity of EGF-PE35KDEL Chimeric Toxin on Hela Cell
     EGF-PE35KDEL嵌合毒素对Hela细胞的毒性作用
短句来源
     Conclusion:Chimeric toxin EGF-PE35KDEL had cytotoxicity to Hela cells which over expressed EGFR in vitro.
     结论:EGF-PE35KDEL嵌合毒素对体外表达EGFR的Hela细胞有杀伤作用。
短句来源
     In this paper we describe in detail study on the heredity stability of a recombinant plasmid pET30a/hsBLyS in Ecoli BL21 and construction 、 expression and activity detection of the chimeric toxin hsBLyS-PE40.
     本论文详细介绍:本室构建的重组pET30a/hsBLyS质粒在BL21(DE3)菌株中的稳定性观察,嵌合毒素hsBLyS-PE40的基因构建、表达及活性检测。
短句来源
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  重组毒素
     Preparation of Specific McAb-PE40 Chimeric Toxin and Its Kill Action to Cryptosporidium Parvum
     特异性单抗-PE40重组毒素的制备及其对隐孢子虫杀灭作用研究
短句来源
     Primary determination for bioactive and construction of DAB389-Linker-LHRH chimeric toxin
     DAB389-Linker-LHRH重组毒素的构建及生物活性的初步测定
短句来源
     LHRH-PE40 are a kind of chimeric toxin constructed by fusing PE40 with LHRH.
     1,本研究首先用体外细胞培养技术观察绿脓杆菌外毒素A –黄体生成激素释放激素重组毒素注射剂(LHRH-PE40)对宫颈癌Hela细胞系生长的影响。
短句来源
     Conclusion:In this study,construction of recombinant plasmid encoding chimeric toxin Stx2A LHRH and its expression were completed successfully by molecular biology technique. The finding could open up new vistas in the study on targeted drugs.
     结论 :利用分子生物学技术成功地构建了带有 Stx2 A- LHRH重组毒素基因的质粒 ,使其表达 Stx2 A- LHRH重组毒素成为可能 ,为进一步研究导向药物奠定了基础。
短句来源
  “chimeric toxin”译为未确定词的双语例句
     The purified recombinant chimeric toxin was found to effectively inhibit the prolifieration of glioblastoma multiforme cells bearing high affinity hIL-13 receptors,and resulted in dose-dependent relationship with 50% inhibition concentration (IC_ 50 ) of 5×10 -11 mol/L.
     进一步活性检测发现 ,该融合蛋白对多型性胶质母细胞瘤细胞系U2 5 1的生长有较强的抑制作用 ,且作用存在剂量相关性 ,其半数抑制浓度(IC50 )约为 5× 10 -11mol/L。
短句来源
     Primary determination for activity and expression of Stx 2a’-LHRH chimeric toxin
     重组志贺毒素的表达及活性的初步测定
短句来源
     IL-2-PE40 is a recombinant chimeric toxin designed to kill cells with IL-2 receptors.
     探讨白细胞介素2(IL-2)与绿脓杆菌外毒素组成的融合蛋白──IL-2-PE40对小鼠异基因骨髓移植的影响。
短句来源
     through progress. The Food and Drug Administration just approved the Mabs Rituximab, Tractuzumab and the Chimeric toxin DAB389IL? 2. These exciting results suggest that Targeting therapy may be applied in human malignant therapy.
     20余年来 ,肿瘤的导向治疗研究经历了高潮和低潮的多次反复 ,终于在近几年取得突破性进展 ,以Ritux imab、Tractuzumab和DAB389IL 2为代表的抗肿瘤导向治疗制剂已经通过FDA批准上市 ,展示出肿瘤导向治疗的光明前景
短句来源
     ConclusionProkaryotic expression system can be recruited to produce recombinant chimeric toxin DT389-hIL-13. The results may lay a foundation for preparing specific the agent targets for tumors overexpressing IL-13 receptor.
     结论 成功构建了融合蛋白DT389 hIL 13的表达质粒 ,在细胞水平对表达产物进行了初步的活性检测 ,为进一步研制特异性的抗胶质瘤药物打下了基础。
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  chimeric toxin
To design a more potent PE-based EGF-receptor-targeting toxin, a chimeric toxin, named EGF-PE(Δ34-220), which had most of the Ia domain deleted but retained amino acid residues 1-33 and 221-252 of this domain, was constructed.
      
A target-specific chimeric toxin composed of epidermal growth factor and Pseudomonas exotoxin A with a deletion in its toxin-bin
      
The chimeric toxin was also significantly less cytotoxic against endothelial cells.
      
Purification of the Chimeric Toxin and Testing of the Enzymatic Activity.
      
In this type of chimeric toxin, the variable regions of the heavy and light chain are held together by a linking peptide.
      
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It has recently been shown that chimeric toxin composed of IL2 fused tp PE40, a mutant form of Pseudomonas Exotoxin A devoid of its native cell recognition and binding domain was cytotoxic to IL-2 receptor bearing cells. We here amplified the gene IL-2(60), which cedes for the N-terminal 1-60 amino acids of human IL-2 by PCR. After that, we fused it to PE40 and the new chimeric protein IL-2(60)-PE40 was expressed in E. coli. SDS-PAGE revealed that IL-2(60)-PE40 chimeric protein accounts for...

It has recently been shown that chimeric toxin composed of IL2 fused tp PE40, a mutant form of Pseudomonas Exotoxin A devoid of its native cell recognition and binding domain was cytotoxic to IL-2 receptor bearing cells. We here amplified the gene IL-2(60), which cedes for the N-terminal 1-60 amino acids of human IL-2 by PCR. After that, we fused it to PE40 and the new chimeric protein IL-2(60)-PE40 was expressed in E. coli. SDS-PAGE revealed that IL-2(60)-PE40 chimeric protein accounts for more than 18% of total cell proteins. As the region IL-2 binds with its receptor was defined in the N-terminal residues 8-54 of IL-2, such fusion proteins will have the same activity with IL-2-PE40. Following primany purification, IL-2 (60)-PE40 was shown to be very toxic to IL-2 receptor-positive cells but non measurable effect on the cells lacking IL-2 receptors. Such a structure has not been reported by now. The fusion protein is useful for suppressing the immune response in cases of rejection of allografts and organ transplants and as therapeutic agents for the treatment of IL-2 receptor related diseases scuh as autoimmune disease, ATL(adult T-cell leukemia), et al.

CLONINGANDEXPRESSIONOFTHEGENECODINGFORIL-2(60)-PE40,AMOLECULARTARGETEDPROTEINZhangMeng(张萌)ZhaoXue(赵雪)LiHuanlou(李焕娄)andLuSheng...

IL-2-PE40 is a recombinant chimeric toxin designed to kill cells with IL-2 receptors.The in vitro experimental results showed that IL-2-PE40 was able to selectively inhibit alloresponsive cells in allogeneic mixed lymphocyte culture(MLC),eliminate cytotoxic effector cells generated in MLC,and maintain the ConA inducing mitogenic response of the nonactivated cells.The growth of CFU-GM did not inhibited by IL-2-PE40.Administration of IL-2-PE40 to mice underwent allogeneic bone marrow transplantation could...

IL-2-PE40 is a recombinant chimeric toxin designed to kill cells with IL-2 receptors.The in vitro experimental results showed that IL-2-PE40 was able to selectively inhibit alloresponsive cells in allogeneic mixed lymphocyte culture(MLC),eliminate cytotoxic effector cells generated in MLC,and maintain the ConA inducing mitogenic response of the nonactivated cells.The growth of CFU-GM did not inhibited by IL-2-PE40.Administration of IL-2-PE40 to mice underwent allogeneic bone marrow transplantation could prolong their survival.

探讨白细胞介素2(IL-2)与绿脓杆菌外毒素组成的融合蛋白──IL-2-PE40对小鼠异基因骨髓移植的影响。实验结果表明体外应用IL-2-PE40能够选择性抑制混合淋巴细胞培养(MLC)中的异基因反应细胞,清除MLC诱导产生的细胞毒效应细胞,保留未活化细胞对ConA诱导的丝裂原反应;体内应用IL-2-PE40能延长异基因骨髓移植小鼠的存活期,而对CFU-GM产率无明显影响。

The fragments of truncated diphtheria toxin(containing 389 amino acids of N terminus,DT 389 )and full length human IL 2 gene were amplified by PCR technique respectively The two fragments were linked together and then inserted into prokaryotic expression vector pET3a The fusion gene encoding the recombinant chimeric toxin was transformed into E Coli BL21 strain and the expressed fusion protein was purified to test the cytotoxicity...

The fragments of truncated diphtheria toxin(containing 389 amino acids of N terminus,DT 389 )and full length human IL 2 gene were amplified by PCR technique respectively The two fragments were linked together and then inserted into prokaryotic expression vector pET3a The fusion gene encoding the recombinant chimeric toxin was transformed into E Coli BL21 strain and the expressed fusion protein was purified to test the cytotoxicity to HUT 102 cells line by 3H Leucine incorporation SDS PAGE analysis showed that the molecular weight of the recombinant chimeric toxin is about 58 kD The purified recombinant chimeric toxin was found to inhibitory the protein synthesis of HUT 102 cells which bearing high affinity IL 2 receptors effectively,and resulted in dose responsive relationship with 50% inhibitory concentration(IC 50 )of 3 3×10 -11 mol/L These results lay a foundation for preparing the therapeutic agent toward tumors highly expressing IL 2 receptors and its related diseases

应用PCR技术分别扩增出编码白喉毒素氨基端 389个氨基酸 (DT3 89)的基因片段及人IL 2全基因 ,将两基因串连插入 pET3a载体 ,构建成含有DT3 89 IL 2融合基因的表达载体 ,转化大肠杆菌BL2 1,经表达、纯化后 ,用3 H Leucine掺入法测定其对HUT 10 2细胞的蛋白合成抑制作用。SDS PAGE电泳分析表明 ,表达产物分子质量 (Mr)约为 5 8kD ;重组嵌合毒素能够特异性地抑制高表达IL 2受体的HUT 10 2细胞的蛋白生物合成 ,且有一定的剂量反应关系 ,其细胞半数抑制浓度 (IC50 )约为 3 3× 10 -11mol/L。为进一步研制特异性的抗IL 2受体高表达肿瘤和相关疾病的药物打下了基础。

 
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