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special primer
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  特异性引物
     Methods:A pairs of primer were designed according to echinococcus granulosus gene fragment clone and sequence analysis special primer as:P1 5'-GGAATGGAGAGAAGTTAC-3',P2 5'-GCAACCTCCGGAACTTGC-3'.
     方法 :根据细粒棘球蚴基因片断克隆与序列分析 ,设计了一对特异性引物 ,P15′—GGAATGGAGAGAAGTTAC— 3′,P2 5′—GCAACCTCCGGAACTTGC— 3′。
短句来源
     3、 The porcine IL-2 and IL-4 genes were amplified from the library by special primer, the PCR product was cloned into the pMD18-T vector and sequenced.
     3、用特异性引物在文库中扩增到猪IL-2和IL-4基因,并成功插入到pMD18-T载体。
短句来源
     Rapid detection of K-ras gene point mutation in pancreatic adenocarcinonia by sequence special primer PCR
     顺序特异性引物法快速检测胰腺癌K-ras基因点突变
短句来源
     METHODS: The genotypes of 90 Chinese Han in Guangdong and 104 Sherpas in Tibet were analyzed by sequence special primer polymerase chain reaction(SSP-PCR) sequencing the surfactant protein A gene.
     方法:应用序列特异性引物-聚合酶链反应(polymerase chain reac-tion-sequence specific primer,SSP-PCR)方法,对90名广东汉族人和104例西藏夏尔巴人SP-A基因进行检测。
短句来源
     Methods The viral genotype was identified by special primer multiple PCR and the HBV DNA level was determined by quantitative PCR on fluorescence assay.
     方法采用型特异性引物多重PCR方法对徐州地区160例CHB患者的HBV进行基因分型,用荧光定量PCR方法检测HBV DNA水平。
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  “special primer”译为未确定词的双语例句
     3. A 1200bp fragment was amplified from a salinity resist rice variety, liaoyan 241 by RT-PCR using osMSRMK2 gene special primer.
     3.利用RT-PCR方法,用水稻促分裂原活化蛋白激酶基因osMSRMK2特异引物从水稻品种辽盐241中扩增到约1200bp的片段。
短句来源
     5. A 1500bp fragment was amplified from a salinity resist rice variety, liaoyan 241 by RT-PCR using osMAPK4 gene special primer.
     5.利用RT-PCR方法,用水稻促分裂原活化蛋白激酶基因osMAPK4特异引物从水稻品种辽盐241中扩增到约1500bp的片段。
短句来源
     4. A 2500bp fragment was amplified from a sality resist rice variety, liaoyan 241 genomic DNA by PCR using osMSRMK2 gene special primer.
     4.利用PCR方法,用水稻促分裂原活化蛋白激酶基因osMSRMK2特异引物从水稻品种辽盐241基因组DNA中扩增到约2500bp的片段。
短句来源
     The results were as follows: 1. The application of SRAP in peanut molecular analysisSRAP (Sequence-related Amplified Polymorphism) is a new marker system based on PCR and preferely amplifies ORFs (open reading frames) by special primer design.
     SRAP(Sequence-related Amplified Polymorphism)是一种新型的基于PCR的标记系统,通过独特的引物设计对ORFs(open reading frames)进行扩增。
短句来源
     The neutralization titer was 1:8.With the special primer,the major antigen coding region sequences of E2 gene about 250 bp was obtained by RT-PCR. The identity of nucleotide sequence and amino acid sequences of the major antigen coding region sequences of E2 gene of the isolated strains was 79.9%~87.9% and 77.7%~86.6%.
     通过 RT -PCR 扩增出猪瘟病毒约250 bp的E2蛋白主要抗原编码区序列,其与几株已发表毒株序列的核苷酸及氨基酸同源性分别为79.9%~87.9%,77.7%~86.6%,与Alfort 株同属于基因二群。
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  相似匹配句对
     Special
     特别报道
短句来源
     Development of Special Low Energy Primer
     低能导爆索专用起爆具的研制
短句来源
     Special Nitriding
     特殊渗氮
短句来源
     SPECIAL PHENOLIC RESIN FOR USE IN PRIMER OF CARBON-FILM RESISTOR
     碳膜电阻器底漆专用酚醛树脂
短句来源
     Research of New Primer
     新型起爆药柱的研制
短句来源
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The glutathione peroxidase cDNA has been acquired by reverse transcriplate-polymerase chain reaction (RT-PCR). mRNA was isolated from the culture liver cancer cells firstly, then trancriplate if into cDNA with AMV reverse transcriptase. By using this cDNA as PCR template and two synthetic special primers, we get about 600 bp DNA fragment after 35 circles. The result is identical with our expectation.

本文应用反转录-聚合酶链反应成功的扩增了人谷胱甘肽过氧化物酶的互补脱氧核糖核酸(cDNA)。该法首先从人肝癌细胞中提取信使核糖核酸,在反转录酶的作用下生成cDNA,以此CDNA作为PCR的模板,利用人工合成的两个特异引物,进行35次循环的扩增。电泳结果表明,扩增片段的大小约为600 bp,与文献结果一致,为该酶基因的表达及生产奠定了基础。

The special primers Of p53 exon 7 as wed as HPV16 E6 and E7 ORFs (Opening Rending Frame) were used with PCR, PCR-SSCP technique, and 35 specimens of cervical carcinoma were examined. The results were as follows: ① HPV16 E6, E7 DNA was found in 25/35 specimens (71. 4%),which proved again HPV16 Infection an important event in cervical carcinogenesis. However only 11/35 (31.42% ) bad E6 and E7 ORFs simultaneously, 3/35 (8. 57%) and 11/35 (31. 42% ) had only E6 or E7 respectively. ② No mutation and LOH (Loss...

The special primers Of p53 exon 7 as wed as HPV16 E6 and E7 ORFs (Opening Rending Frame) were used with PCR, PCR-SSCP technique, and 35 specimens of cervical carcinoma were examined. The results were as follows: ① HPV16 E6, E7 DNA was found in 25/35 specimens (71. 4%),which proved again HPV16 Infection an important event in cervical carcinogenesis. However only 11/35 (31.42% ) bad E6 and E7 ORFs simultaneously, 3/35 (8. 57%) and 11/35 (31. 42% ) had only E6 or E7 respectively. ② No mutation and LOH (Loss of Heterozygote) of p53 exon 7 were found in allof 35 specimens. Additionally in the present study, we developed a non-isotopic PCR-SSCP method.

THEDETECTIONOFP53GENEINHUMANCERVICALCARCINOMAWITHANDWITHOUTHUMANPAPILLOMAVIRUSINFECTIONSunYi(孙毅);SiLusheng(司履生)SunYi;SiLushen...

iang Dezhao(Department of Hematology, Hunan Medical University)A gene of human stem cell factor was amplified from cultured human bone marrow stromacells by reverse transcription-PCR(RT-PCR)with a pair of special primers and recombined withthe vectors pKK233-2 and PUC18.Using analysis of restriction endonuclease mapping,PCR andDNA sequencing,the cloned SCF gene was defined.The over-expression and purification of re-combinant SCF gene and its products are still in progress.

采用逆转录-PCR的方法从骨髓基质细胞中扩增出人干细胞因子基因,并与载体pKK233-2和pUC18重组。经该基因的限制酶酶解图谱分析及DNA序列分析证实。

 
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