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plant lectin
相关语句
  植物凝集素
     This paper summarized the researches on insect-resistance and economic properties of the transgenic tobacco plant transformed by Bt toxin gene, plant lectin GNA,P-lec and AHA gene, protease inhibitor PI-Ⅰ,PI-Ⅱ,MTI and SKTI genes, insect-specific neurotoxin gene, chitnase gene, teratocyte secretory protein gene and other insect-resistant genes. At last, the prospect of the further studies and application of transgenic tobacco was discussed.
     文章从转Bt内毒素基因,植物凝集素GNA,Plec,AHA基因,蛋白酶抑制剂PIⅠ,PIⅡ,MTI,SKTI基因,昆虫特异性神经毒素基因,几丁质酶基因,畸形细胞分泌蛋白基因以及双抗虫基因等方面综述了转基因抗虫烟草的抗虫性、转基因抗虫烟草的经济性状等,展望了转基因抗虫烟草的研究和应用前景,以期对烟草害虫的治理尤其是对其他转基因抗虫作物的培育和研究有借鉴作用。
短句来源
     Study on the Molecular Biology of Plant Lectin
     植物凝集素的分子生物学研究
短句来源
     Plant Lectin and its Application in the Genetic Engineering of Plant Resistance to Insects
     植物凝集素及其在抗虫基因工程中的应用
短句来源
     It were summarized the classification and killing mechanism and the application of the three main insect resistant genes,i e.Bt toxic protein gene,proteinase inhibitor gene and plant lectin on the genetic engineering.
     概述了目前应用于植物抗虫基因工程的苏云金杆菌毒蛋白基因、蛋白酶抑制剂基因和植物凝集素等三类主要基因的分类、杀虫机理及应用现状;
短句来源
     Pinellin is also a plant lectin, as it exhibits a specific hemagglutinating activity to rabbit erythrocytes at a concentration as low as 2ug/ml.
     半夏蛋白也是一种植物凝集素,它与兔红血球有专一的凝集活力,浓度低至每毫升2微克时仍有凝集作用。
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  植物外源凝集素
     Mannose also is the basic component of saliva acid and plant lectin. These two kinds of glycoproteins are parts of plant cell wall components and highly related to disease and insect resistances of plants.
     甘露糖也是聚唾液酸和植物外源凝集素(lectin)的成分,而这两种甘露糖蛋白又是植物的细胞壁成份之一,他们都与植物的抗虫和抗病性有关。
短句来源
  “plant lectin”译为未确定词的双语例句
     The Mechanisms of A New Plant Lectin FRIL from Dolichos Lablab That Preserves Quiescent Hematopoietic Stem Cells in Suspension Culture
     新的豆类凝集素FRIL及其体外维持造血干/祖细胞特性的作用机制
短句来源
     THE PROJECTIONS FROM THE EXTERNAL CUNEATE NUCLEUS TO THE CEREBELLUM OF THE RAT. IMMUNOHISTOCHEMICAL LOCALIZATION OF AXONALLY TRANSPORTED PLANT LECTIN PHA SEOLUS VULGARIS-LEUCOAGGLUTININ(PHA-L)
     大鼠外侧楔核向小脑投射的追踪研究——PHA-L法
短句来源
     The sequence from Wan82-178 show that the cloned fragment is 999bp. Like most plant lectin genes, there is no intron in the amplified sequence.
     根据大豆凝集素基因设计特异性引物,从大豆材料皖82-178(抗)和通山薄皮黄豆甲(感)中获得凝集素基因。
短句来源
     The sequence data showed that the cloned fragment is 781 bp. Like most plant lectin genes,there is no intron in the amplified sequence. The open reading frame of the fragment codes a 23 kD polypeptide which contains 227 amino acid residues and there is an signalsequence with 28 amino acid residues at its NH 2 terminus.
     序列分析表明,克隆到的基因片段大小为781bp,没有内含子,编码1条长227个氨基酸、分子量约23kD的肽链,其中N-端28个氨基酸是信号肽。
短句来源
     Like most plant lectin genes, there is no intron in the amplified sequence. The open reading frame of the fragment codes a 23kD polypeptide which contains 227 amino acid residues and there is a signal sequence with 28 amino acid residues at its NH 2 terminus.
     序列分析表明 ,克隆到的基因片段大小为 781bp,没有内含子 ,编码一条长 2 2 7个氨基酸、分子量约 2 3k D的肽链 ,其中 N-端 2 8个氨基酸是信号肽。
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  plant lectin
We have devised an enrichment strategy in which rat hepatoma cells unable to replace surface membrane receptors of a plant lectin, concanavalin A, are resistant to the cytotoxic effects of this lectin when administered at a nonpermissive temperature.
      
Selective enrichment for temperature-sensitive secretion mutants of mammalian cells using plant lectin, concanavalin A
      
The plant lectin Tetracarbidium conophorum agglutinin II binds to glycoproteins and glycopeptides in a structurally specific manner [Animashaun et al., (1994) Glycoconjugate J.11, 299-303].
      
Previously, we have shown that subagglutinating concentrations of the plant lectin, wheat germ agglutinin (WGA) specifically and irreversibly inhibitedN-formyl-methionyl-leucyl-phenyl-alanine (FMLP)-mediated PMN chemotaxis.
      
How these versatile probes are produced in plants and how they are swiftly and efficiently purified are outlined, and insights into the diversity of plant lectin structures are also given.
      
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A plant protein, designated pinellin, was isolated from the juice of the Chinese traditional medicine pinellia (Pinellia ternata Breit.) and crystallized in a dilute phosphate buffer, pH 6.8, at 28~30℃. It exhibits the activity of terminating early pregnancy. Hypodermic injeotion of 250μg of pinellin per mouse on the 7th day of gestation induced a termination of pregnancy in 50% of the mice tested. Pinellin is also a plant lectin, as it exhibits a specific hemagglutinating activity to rabbit erythrocytes...

A plant protein, designated pinellin, was isolated from the juice of the Chinese traditional medicine pinellia (Pinellia ternata Breit.) and crystallized in a dilute phosphate buffer, pH 6.8, at 28~30℃. It exhibits the activity of terminating early pregnancy. Hypodermic injeotion of 250μg of pinellin per mouse on the 7th day of gestation induced a termination of pregnancy in 50% of the mice tested. Pinellin is also a plant lectin, as it exhibits a specific hemagglutinating activity to rabbit erythrocytes at a concentration as low as 2μg/ml. The molecular weight of pinellin is 44,000 as determined by gel fitration. Pinellin dissociated into subunits in the presence of sodium dodecyl sulfate or guanidine hydrochloride. The amino acid composition and N-terminal amino acid residues of pinellin have been analysed. It is devoid of hexose. There are suggestions that pinellin may represent a family of isoproteins with very similar molecular size and structure.

从中草药半夏(Pinellia ternata Breit.)的鲜汁中分离出一种植物蛋白质组分,定名为半夏蛋白。它在稀磷酸缓冲液,pH 6.8,28~30℃结晶。它具有抑制早期妊娠的活力。给怀孕七天的小白鼠每只皮下注射250微克半夏蛋白,50%注射过的小白鼠引起流产。半夏蛋白也是一种植物凝集素,它与兔红血球有专一的血凝活力,浓度低至每毫升2微克仍有凝集作用。用凝胶过滤法测定,半夏蛋白分子量为44,000。十二烷基硫酸钠或胍盐存在下,半夏蛋白解聚成亚基。测定了半夏蛋白的氨基酸组成和N-末端氨基酸残基。半夏蛋白不含己糖。有迹象表明半夏蛋白可能是其分子大小、结构与性质极为相近的一组蛋白。

A plant protein, designated pinellin, was isolated from the juice of the Chinesetraditional medicine pinellia (Pinellia ternata Breit.) and crystallized in a dilutephosphate buffer, pH 6.8, at 28~30℃. It exhibits the activity of terminating earlypregnancy. Hypodermic injection of 250μg of pinelliu per mouse on the 7th day ofgestation induced a termination of pregnancy in 50% of the mice tested. Pinellinis also a plant lectin, as it exhibits a specific hemagglutinating activity to rabbiterythrocytes at...

A plant protein, designated pinellin, was isolated from the juice of the Chinesetraditional medicine pinellia (Pinellia ternata Breit.) and crystallized in a dilutephosphate buffer, pH 6.8, at 28~30℃. It exhibits the activity of terminating earlypregnancy. Hypodermic injection of 250μg of pinelliu per mouse on the 7th day ofgestation induced a termination of pregnancy in 50% of the mice tested. Pinellinis also a plant lectin, as it exhibits a specific hemagglutinating activity to rabbiterythrocytes at a concentration as low as 2μg/ml. The molecular weight of pinellinis 44,000 as determined by gel filtration. Pinellin dissociated into subunits in thepresence of sodium dodecyl sulfate or guanidine hydrochloride. The amino acidcomposition and N-terminal amino acid residues of pinellin have been analysed. It isdevoid of hexose. There are suggestions that pinellin may represent a family ofisoproteins with very similar molecular size and structure.

从中草药半夏(Pinellia ternata Breit.)的鲜汁中分离出一种植物蛋白质组分,定名为半夏蛋白。它在稀磷酸缓冲液,pH6.8,28~30℃结晶。它具有抑制早期妊娠的活力。给怀孕七天的小白鼠每只皮下注射250微克半夏蛋白,50%注射过的小白鼠引起流产。半夏蛋白也是一种植物凝集素,它与兔红血球有专一的血凝活力,浓度低至每毫升2微克仍有凝集作用。用凝胶过滤法测定,半夏蛋白分子量为44,000。十二烷基硫酸钠或胍盐存在下,半夏蛋白解聚成亚基。测定了半夏蛋白的氨基酸组成和N-末端氨基酸残基。半夏蛋白不含已糖。有迹象表明半夏蛋白可能是其分子大小、结构与性质极为相近的一组蛋白。

A novel plant lectin was isolated and phrified from the tubers of Typhonium gigantum Engl, a traditional Chinese medicine.Purification was carried out by affinity chromatography on chicken ovomucoid-Sepharose and futher by chromatography on Sepharose 6B. The purified Typhonium gigantum Lectin (TGL) is homogeneous proteins exhibiting a single band by PAGE at pH 8.9 and pH 4.3 and by disc-SDS-PAGE. Their isoelectric point lies at pH 4.6. The molecular weight of 22,500 and 24,000 was determined by disc...

A novel plant lectin was isolated and phrified from the tubers of Typhonium gigantum Engl, a traditional Chinese medicine.Purification was carried out by affinity chromatography on chicken ovomucoid-Sepharose and futher by chromatography on Sepharose 6B. The purified Typhonium gigantum Lectin (TGL) is homogeneous proteins exhibiting a single band by PAGE at pH 8.9 and pH 4.3 and by disc-SDS-PAGE. Their isoelectric point lies at pH 4.6. The molecular weight of 22,500 and 24,000 was determined by disc SDS-PAGE and by gel filtration respectively. By phenol-sulfuric acid method, it showed a sugar content of approximately 7.5%. The lectins had a typical ultraviolet absorption spectrum. The amino acid compositions indicated that they contein high amounts of aspartic acid, serin and threoine, but a little of cysteine and methionine. The DNS method of N-terminal aminino acid analysis of TGL showed the presence of alanine.And TGL exhibited a specific hemagglutinating activity to rabbit erythrocytes at a concentration as low as 1 ug/ml.

用鸡卵类粘蛋白—Sepharose作亲和吸附剂,以线性离子梯度洗脱后,活性部分进一步于Sepharose 6B 凝胶过滤,所得凝集素制品经连续PAGE(pH8.9和pH 4.3)以及Disc—SDS—PAGE鉴定,均呈单一蛋白带,用SDS—PAGE和凝胶渗透色谱测其分子量分别为22,500和24,000.凝胶等电聚焦测其pI为4.6,氨基酸组成分析表明该凝集素富含Asp,而含硫氨基酸甚少,DNS—聚酰胺薄膜层析分析其N—未端氨基酸残基为Ala.紫外吸收光谱表明该凝集素在pH7.5、0.1M PBS下,其吸收波峯在275nm,低谷在250nm.酚-硫酸法测其总糖量为7.5%.凝集素浓度低至每毫升1微克时,对免红细胞仍有凝集作用.

 
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