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mononuclear bone marrow cells
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  骨髓单个核细胞
     Objective:To investigate the effects of transplanting mononuclear bone marrow cells (MBMCs) in treatment of necrotic cardiac muscle in dilated cardiomyopathic rats.
     目的 :研究大鼠骨髓单个核细胞 (m ononuclear bone marrow cells,MBMCs)移植对扩张型心肌病 (dilated cardiomy-opathy,DCM)大鼠心肌修复的作用。
短句来源
     The Animal and Clinical Study on Mononuclear Bone Marrow Cells Transplantation in Treatment of Dilated Cardiomyopathy
     骨髓单个核细胞移植治疗扩张型心肌病的动物与临床研究
短句来源
     Objective:To investigate the therapeutic effect of mononuclear bone marrow cells(MBMCs) transplanting in treatment of myocardial infarction.
     目的 :探讨大鼠骨髓单个核细胞 (m ononuclear bone m arrow cells,MBMCs)移植方法对梗死心肌的治疗效果。
短句来源
     Mononuclear bone marrow cells transplanting in treatment of myocardial infarction
     大鼠骨髓单个核细胞移植治疗心肌梗死
短句来源
     The Changes of Ischemic Myocardium in Rabbits before and after Mononuclear Bone Marrow Cells Implantation
     骨髓单个核细胞移植前后兔缺血心肌的变化
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  “mononuclear bone marrow cells”译为未确定词的双语例句
     Intracoronary injection of mononuclear bone marrow cells in acute myocardial infarction
     冠状动脉内注射单核骨髓细胞治疗急性心肌梗死
短句来源
     Impact of autologous mononuclear bone marrow cells transplantation on early and med-term outcomes on the cardiac function and myocardial metabolic in patients with myocardial infaction
     自体骨髓干细胞移植治疗心肌梗死患者心肌代谢及收缩功能的变化
短句来源
     Impact of autologous mononuclear bone marrow cells transplantation on early and medterm outcomes of the cardiac function and myocardial metabolism in patients with myocardial infaction
     心肌梗死患者自体骨髓干细胞移植后近、中期心肌代谢及收缩功能的变化
短句来源
     As a sort of multipotential former body cells of various cells and tissue , bone marrow stem cells have no explicit morphological character, it is mononuclear bone marrow cells (MBMCs). It has the ability to self-renew and differentiate and can also release some cytokines to enhance angiogenesis.
     骨髓干细胞是多种细胞及组织的多潜能前体细胞,没有明确的形态学特征,为单个核细胞(mononuclear bone marrow cells,MBMCs),它具有自我更新和分化潜能,又能释放某些细胞因子促进血管新生。
短句来源
     Hu-man mononuclear bone marrow cells were infected repeatly with this high titer virus vectorunder stimulation of hematopoietic growth factors IL-3,IL-6 and SCF,Southern blot hy-bridization analysis showed that β-globin gene and its enhancer had been integrated into thegenome of human hematopoietic cells.
     用此高滴度的病毒载体,在加入造血生长因子的情况下,重复感染人骨髓单核细胞,Southern印迹杂交显示人β-珠蛋白基因及其增强子已完整地整合到人骨髓造血细胞基因组上。
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  相似匹配句对
     Chondroblastoma of bone
     骨的软骨母细胞瘤
短句来源
     Bone breakage
     老母鸡的骨折
短句来源
     The bone marrow mononuclear cell population collected at the Percoll interface.
     PBS洗涤后的骨髓细胞悬液Percoll分离工作液后,分为血小板层、白细胞层和红细胞层。
短句来源
     Mononuclear bone marrow cells transplanting in treatment of myocardial infarction
     大鼠骨髓单个核细胞移植治疗心肌梗死
短句来源
     The differentiation of human bone marrow mononuclear cell into hepatocyte in vitro
     人骨髓单个核细胞向肝细胞诱导分化的体外研究
短句来源
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  mononuclear bone marrow cells
Cardiac contractility after transplantation of autologous mononuclear bone marrow cells in patients with myocardial infarction
      
Autologous Mononuclear Bone Marrow Cells during Reparative Regeneratrion after Acute Myocardial Infarction
      
In model experiments with KM3 cells admixed to mononuclear bone marrow cells, the individual antibodies each removed 2.8-3.1 logs and 3.6-4.1 logs of tumor cells with one and two purging cycles, respectively.
      
This level of Cd also caused a fivefold increase in the number of multinucleated osteoclast-like cells formed during invitro incubation of progenitor-enriched mononuclear bone marrow cells.
      
Under optimal conditions one tumor cell among one million mononuclear bone marrow cells can be detected by immunocytochemistry or molecular methods.
      
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Retroviral vectors containing bacterial neomycin-resistant (neoR) gene were transfered into primary human hematopoietic progenitor cells (HHPC). To obtain HHPC rich populations, mononuclear bone marrow cells were collected using Ficoll density gradient centrifugation. These cells were preincubated and cocultivated with irradiated (1500 cGy) viral producer cells for 24 hours, then plated in semisolid medium with increasing G418 concentrations (0 to 1000μg/ml). The results showed 9.7%- 17.8%...

Retroviral vectors containing bacterial neomycin-resistant (neoR) gene were transfered into primary human hematopoietic progenitor cells (HHPC). To obtain HHPC rich populations, mononuclear bone marrow cells were collected using Ficoll density gradient centrifugation. These cells were preincubated and cocultivated with irradiated (1500 cGy) viral producer cells for 24 hours, then plated in semisolid medium with increasing G418 concentrations (0 to 1000μg/ml). The results showed 9.7%- 17.8% of them were G418 resistant CFU-GM colonies. Furthermore,the neoR gene from viral producer cells and long-term marrow cultures was directly detected by polymerase chain reaction (PCR),and the results showed the presence of neoR gene in the transfected cells. These studies demonstrate that the neoR gene can be successfully transfected into normal hematopoietic stem / progenitor cells at high efficiency using the retrovirus vectors. Therefore, it would be an important step towards human gene therapy and the study of human hematopoiesis.

本文应用逆转录病毒载体介导基因转移法将含有新霉素抗性(neo~R)基因的双拷贝逆转录病毒载体pN2A转入正常人造血祖细胞。应用Ficoll分层液(比重1.064)富集人造血干、祖细胞,预培养后与已经照射的生产重组病毒包装细胞株共同培养24小时,经含G418(1000μg/ml)体外半固体培养,可见具有抗性的CFU-GM集落生长。应用PCR方法检测转染细胞中neo~R基因的转移和表达,在生产逆转录病毒的辅助细胞株PA317/N2及体外液体培养的骨髓细胞中均可扩增出neo~R基因的DNA片段,进一步证实了neo~R基因在正常人造血干、祖细胞的有效转移和表达。

Transferring β-globin gene and its enhancer into human hematopoietic cells is the basisfor applying β-thalassemia gene therapy in clinical practice.We isolated ecotropic virus pro-ducing clones and amphotropic virus producing clones by using a replication-defective retro-virus vector containing β-globin gene and its enhancer to transfect ecotropic packaging cellline -2 and amphotropic packaging cell line PA317.Then by ping-ponging supernatant in-fection between ecotropic producer clone and amphotropic producer...

Transferring β-globin gene and its enhancer into human hematopoietic cells is the basisfor applying β-thalassemia gene therapy in clinical practice.We isolated ecotropic virus pro-ducing clones and amphotropic virus producing clones by using a replication-defective retro-virus vector containing β-globin gene and its enhancer to transfect ecotropic packaging cellline -2 and amphotropic packaging cell line PA317.Then by ping-ponging supernatant in-fection between ecotropic producer clone and amphotropic producer clone, we obtained hightiter amphotropic PA317 producer clones with the highest titer up to 5.9×106 CFU/ml.Hu-man mononuclear bone marrow cells were infected repeatly with this high titer virus vectorunder stimulation of hematopoietic growth factors IL-3,IL-6 and SCF,Southern blot hy-bridization analysis showed that β-globin gene and its enhancer had been integrated into thegenome of human hematopoietic cells.

用重组人β-珠蛋白基因及其增强子的逆转录病毒载体转染-2细胞及PA317细胞,分离出产病毒粒子的-2和PA317克隆。用这两种细胞克隆进行乒乓上清感染,得到能产生高滴度逆转录病毒载体的PA317细胞克隆,其滴度达5.9×10~6CFU/ml。用此高滴度的病毒载体,在加入造血生长因子的情况下,重复感染人骨髓单核细胞,Southern印迹杂交显示人β-珠蛋白基因及其增强子已完整地整合到人骨髓造血细胞基因组上。

Objective: Our study was designed to test the therapeutic effectiveness of transplanting mononuclear bone marrow cells into myocardial infarction scar and the periphery, and detect the expression of important angiogenic genes involved in the processes of collateral development. Methods: Myocardial infarction scar was induced by cryoinjury. After 4 weeks, twenty-four hours before cell transplantation, bone marrow was aspirated from the right thigh bone and mononuclear BMCs...

Objective: Our study was designed to test the therapeutic effectiveness of transplanting mononuclear bone marrow cells into myocardial infarction scar and the periphery, and detect the expression of important angiogenic genes involved in the processes of collateral development. Methods: Myocardial infarction scar was induced by cryoinjury. After 4 weeks, twenty-four hours before cell transplantation, bone marrow was aspirated from the right thigh bone and mononuclear BMCs were isolated by Ficoll desity gradient centrifugation. Then the mononuclear BMCs(n=8) or IMDM culture medium(n=8) were transplanted into infarction scar and the periphery. Four weeks after cell transplantation, histological study was performed to assess the characteristics and compare the number of microvessels of the samples of infarction scar and the periphery in both groups, and compare the differences of angiogenic genes expression among several important growth factors by western blot. Results: In the mononuclear BMCs transplantation group, the microvessel densities were (8.56±1.32)/0.2mm 2 and (12.449±1.97)/ 0.2 mm 2 in the infarction scar and the periphery, respectively, which were significantly higher than those in the control group, ie, (4.92±1.61)/0.2mm 2 (P<0.05) and (6.84±2.18)/0.2mm 2 (P<0.05), respectively. Western blot analysis demonstrated that mononuclear BMCs transplantation was capable to increase the levels of VEGF, FGF and Agiopoietin-Ⅰexpression in the infarction scar and the periphery, respectively, compared with those in the control group (P<0.05). Conclusion:The expression of some important angiogenesis-promoting genes regulated in relatively high levels in infarction scar and the periphery after mononuclear BMCs transplantation. Furthermore, in the periphery of infarction scar, the angiogenesis process induced by mononuclear BMCs is more effective. These findings imply that transplantation of total, unfractionated bone marrow is appealing because of its apparent simplicity and therapeutic effectiveness in upregulating the expression of important angiogenesis-promoting genes.

目的 :探讨骨髓单个核细胞植入心肌梗死及其周边区对心肌梗死的治疗作用 ,促进血管新生及相关基因表达情况。方法 :以冷冻损伤的方法建立新西兰种家兔心梗模型 ,4周后 ,将提取分离的骨髓单个核细胞 ,植入心梗及心梗周边区。分析心梗及心梗周边区病理组织学特点 ,比较区域内微血管密度 ,并以Westernblot研究骨髓单个核细胞移植对心梗及心梗周边区血管生成相关基因表达的影响。结果 :(1)骨髓单个核细胞植入心梗及心梗周边区减少移植区域瘢痕化 ,增加微血管密度 ;(2 )骨髓单个核细胞植入心梗及心梗周边区使某些重要血管生成相关基因 ,VEGF ,FGF及Angiopoietin -Ⅰ表达上调 ,于心梗周边区作用更加显著。结论 :骨髓单个核细胞植入心梗及心梗周边区可以减少移植区域瘢痕化 ,增加微血管密度 ,上调心梗及心梗周边区重要血管生成相关基因。

 
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