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botulinum neurotoxin type a
相关语句
  a型肉毒毒素
     Expression, purification and binding activity analysis of human ScFv specific against botulinum neurotoxin type A
     抗A型肉毒毒素人源单链抗体的表达、纯化及结合活性分析
短句来源
     Objective To express, purify and analyze the antigenicity of the heavy chain from C-terminal binding domain of botulinum neurotoxin type A(BoNT/AHc-C).
     目的A型肉毒毒素重链C-端结合区(BoNT/A Hc-C)的PCR拼接合成、重组表达与免疫原性分析。
短句来源
     Immunogenicity and overexpression of PCR-synthesizing gene of heavy chain from C-terminal binding domain of botulinum neurotoxin type A
     PCR合成的A型肉毒毒素重链C端结合区基因的高效表达与免疫原性分析
短句来源
     Objective: To express and purify human ScF v specific against botulinum neurotoxin type A(BoNT/A), and analyze its binding a c tivity.
     目的 :实现特异性抗A型肉毒毒素人源单链抗体 (ScFv)的表达与纯化 ,并进行结合活性分析。
短句来源
     Conclusion The PCR system is specific and sensitive for the identification of botulinum neurotoxin type A.
     结论:建立的检测方法特异性强、灵敏度高,可以用于A型肉毒毒素基因的快速筛查。
短句来源
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  肉毒神经毒素a
     Generation and characterization of monoclonal antibodies against botulinum neurotoxin type A (BoNT/A)
     抗肉毒神经毒素A(BoNT/A)单克隆抗体的制备与鉴定(英文)
短句来源
  “botulinum neurotoxin type a”译为未确定词的双语例句
     Detection of the Gene Encoding Botulinum Neurotoxin Type A by PCR
     A型肉毒神经毒素基因的PCR检测
短句来源
     Analysis of genome sequence of Clostridium botulinum neurotoxin type A and its prediction of related B cell epitopes
     A型肉毒神经毒素(BoNT/A)基因序列分析及其B细胞表位预测
短句来源
     AIM: To generate the monoclonal antibodies (mAbs) against botulinum neurotoxin type A (BoNT/A).
     目的 :制备抗肉毒毒素A(BoNT/A)的单克隆抗体 (mAb)。
短句来源
     Preliminary Identification of Neutralizing Epitopes of Botulinum Neurotoxin Type a by Ribosome Display Technology
     应用核糖体展示技术对A型肉毒梭菌毒素保护性抗原表位的初步研究
短句来源
     Aim: In order to learn nucleotide sequences of Clostridium botulinum neurotoxin type A(BoNT/A) and deduce its amino acid sequences, the authors analyzed the gene of the toxin .
     对国内A型肉毒杆菌毒素基因测序,分析了解毒素基因结构并推测其氨基酸序列。
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  botulinum neurotoxin type a
The use of Botulinum neurotoxin type A (BoNT/A) in the lower urinary tract was pioneered as early as 20 years ago with injections into the urethral sphincter reducing bladder voiding pressures, urethral pressures, and post-void residual urine.
      
The use of botulinum neurotoxin type A (BoNTA) in urology
      
The binding of125I-botulinum neurotoxin type A is decreased by previous treatment of synaptosomes by neuraminidase and trypsin, and by a preincubation with bovine brain gangliosides or antiserum raised against Torpedo presynaptic plasma membrane.
      
Torpedo electric organ has been used to study the binding of botulinum neurotoxin type A to pure cholinergic synaptosomes and presynaptic plasma membrane.125I-labeled botulinum neurotoxin type A exhibits specific binding to cholinergic fractions.
      
Botulinum neurotoxin type A, the most toxic substance known to mankind, is produced by Clostridium botulinum type A as a complex with a group of neurotoxin-associated proteins (NAPs) through polycistronic expression of a clustered group of genes.
      
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AIM: To generate the monoclonal antibodies (mAbs) against botulinum neurotoxin type A (BoNT/A). METHODS: BALB/c mice were intraperitoneally immunized with purified BoNT/A-Hc, and the splenocytes of immunized mice were fused with myeloma cells Sp2/0. Hybridoma cells were screened by indirect ELISA and monoclonal hybridoma cells was obtained using limited dilution. RESULTS: Three hybridomas, named 4A8, 2F7 and 4F2, producing the mAbs against BoNT/A, were successfully established, and the titer of ascitic...

AIM: To generate the monoclonal antibodies (mAbs) against botulinum neurotoxin type A (BoNT/A). METHODS: BALB/c mice were intraperitoneally immunized with purified BoNT/A-Hc, and the splenocytes of immunized mice were fused with myeloma cells Sp2/0. Hybridoma cells were screened by indirect ELISA and monoclonal hybridoma cells was obtained using limited dilution. RESULTS: Three hybridomas, named 4A8, 2F7 and 4F2, producing the mAbs against BoNT/A, were successfully established, and the titer of ascitic mAbs ranged from 1×10 -4 to 1×10 -6. Identification of subclass showed that all the produced mAbs belonged to IgG1. 4A8 and 4F2 were stable in secreting anti-BoNT/A mAbs through three-month continuous culture and showed high specificity to recombinant BoNT/A-Hc and native BoNT/A. CONCLUSION: Anti-BoNT/A mAbs we generated have high specificity, which laid the foundation for the immunological detection of BoNT/A and clinical treatment of botulism in the future.

目的 :制备抗肉毒毒素A(BoNT/A)的单克隆抗体 (mAb)。方法 :用纯化的重组BoNT/A Hc片段免疫BALB/c小鼠 ,取其脾细胞与骨髓瘤Sp2 /0融合 ,经间接ELISA筛选和克隆化制备杂交瘤细胞系 ,及Western免疫印迹分析等方法对mAb进行特异性鉴定。结果 :获得 3株杂交瘤细胞株 :命名为4A8、2F7和 4F2 ,IgG亚类鉴定均为IgG1,腹水mAb的效价在 1× 10 -4~ 1× 10 -6之间。其中 ,4A8和 4F2能稳定分泌抗BoNT AmAb,并可特异性地识别重组BoNT/A和天然BoNT/A ,特别是 4A8可保护小鼠抵抗 10LD50 BoNT/A的攻击。结论 :成功地制备 3株特异性抗BoNT/AmAb ,并有 1株属于中和性mAb ,为BoNT/A的检测和肉毒中毒的临床治疗奠定了基础

Objective: To express and purify human ScF v specific against botulinum neurotoxin type A(BoNT/A), and analyze its binding a c tivity. Methods: ScFv gene was cloned into prokaryotic exp ression vector pET22b to construct a recombinant expression plasmid, and ScFv w a s expressed in E.coli BL21(DE3) by IPTG induction. Recombinant ScFv was puri fied by IMAC and identified by ELISA. Results: Human ScFv was expressed stably as inclusion body by vector pET22b in E.coli and its ex pression level was beyond...

Objective: To express and purify human ScF v specific against botulinum neurotoxin type A(BoNT/A), and analyze its binding a c tivity. Methods: ScFv gene was cloned into prokaryotic exp ression vector pET22b to construct a recombinant expression plasmid, and ScFv w a s expressed in E.coli BL21(DE3) by IPTG induction. Recombinant ScFv was puri fied by IMAC and identified by ELISA. Results: Human ScFv was expressed stably as inclusion body by vector pET22b in E.coli and its ex pression level was beyond 25% of total bacteria protein. Purity of recombinant S cFv (rScFv)protein was above 95% after a simple IMAC. rScFv Showed good bindin g activity to BoNT/A to compete with antitoxin serum in ELISA. Conclu sion: Recombinant human ScFv specific against BoNT/A could be overexp ressed in E.coli, and showed good binding activity to BoNT/A.

目的 :实现特异性抗A型肉毒毒素人源单链抗体 (ScFv)的表达与纯化 ,并进行结合活性分析。方法 :克隆单链抗体基因ScFv(VH Linker Vk) ,利用pET2 2b表达载体构建重组表达质粒 ,在大肠杆菌BL2 1(DE3)中进行IPTG化学诱导 ,固相亲和层析纯化蛋白 ,ELISA测定其特异结合活性。结果 :pET2 2b可稳定表达人源单链抗体基因 ,表达产物占全菌蛋白的 2 5 % ,表达蛋白全部以包涵体形式存在于胞内。经IMAC纯化 ,蛋白纯度大于 95 %。竞争性ELISA测定结果表明 ,重组抗毒素在体外具有良好的活性 ,可竞争肉毒马血清与肉毒毒素结合。结论 :采用原核表达系统可实现抗A型肉毒毒素人源单链抗体的高效表达 ,重组人源单链抗体具有抗原特异结合活性。

Aim: In order to learn nucleotide sequences of Clostridium botulinum neurotoxin type A(BoNT/A) and deduce its amino acid sequences, the authors analyzed the gene of the toxin .Methods: Firstly, extracting total DNA from Clostridium botulinum type A and then achieving the target gene by amplifying with a pair of specific primers designed by themselves. Secondly, transporting the target gene to Ecoli JM109. Finally, selecting positive E.Coli JM109 containing target gene and analyzing it. Results:...

Aim: In order to learn nucleotide sequences of Clostridium botulinum neurotoxin type A(BoNT/A) and deduce its amino acid sequences, the authors analyzed the gene of the toxin .Methods: Firstly, extracting total DNA from Clostridium botulinum type A and then achieving the target gene by amplifying with a pair of specific primers designed by themselves. Secondly, transporting the target gene to Ecoli JM109. Finally, selecting positive E.Coli JM109 containing target gene and analyzing it. Results: Nucleotide sequences of the genes in the study showed 96% identity with that of BoNT/A from GenBank, but The deduced amino acid sequence of BoNT/A is completely identical. Conclusions: The authors have successfully detected gene sequence of botulinum neurotoxin type A and this will help researchers to produce the neurotoxin by gene engineering and make it possible to diagnose rapidly.

对国内A型肉毒杆菌毒素基因测序,分析了解毒素基因结构并推测其氨基酸序列。提取肉毒杆菌总DNA,利用自行设计的引物对目的基因进行PCR扩增,把目的基因导入E.ColiJM109,筛选含目的基因的阳性克隆菌进行测序和分析,从而了解毒素基因结构并推测其氨基酸序列。对A型肉毒杆菌毒素基因的核苷酸序列成功测序并与GenBank中的相应序列比较,其序列同源性为96%。推测其氨基酸序列同源性为100%。成功地对A型肉毒杆菌毒素的基因进行了测序,这为肉毒杆菌毒素中毒的快速诊断,以及进一步探求以基因工程方法生产此毒素奠定基础。

 
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