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activity site
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  活性中心
    All results show that additional metal ions entered the activity site replacing Zn(II) in the original active site of metalloenzyme, which influenced the enzyme activityAs for B.S.
    结果表明,外加金属离子都能进入上述四种酶的活性中心位点,取代中心位点的Zn(Ⅱ),影响酶活性。
短句来源
    To further substantiate the hypothesis and study the correlation between interchain cross-linking and enzyme activity of protein disulfide isomerase, two point mutants on PDI’s activity site Cys36/38 1 Ser were generated.
    为进一步证实这一假说并研究蛋白质二硫键异构酶分子间交联与其酶活性的之间的相互关系,本文对蛋白质二硫键异构酶分子进行了两处点突变,分别将位于其两个活性中心内的半胱氨酸(Cys36和Cys381)突变为丝氨酸。
短句来源
    crassa similar to the precursors of phytases from Aspergillus niger Aspergillus ficuum, Aspergillus terrus. The peptide was found to possess the conserved sequence motif RHG×R×P of histine acid phosphates, which was the enzyme activity site of microbial phytases.
    crassa基因组中有一长为1507bp的编码基因与黑曲霉、无花果烟曲霉、土曲霉等微生物植酸酶基因的植酸酶编码基因有一定的同源性,且该基因的结构基因中含有微生物植酸酶常有的活性中心保守序列RHG×R×P。
短句来源
    The results of enzyme assay showed that the protein was in inclusion body, with the activity of the supernate only 0.8 U. mL-1. Structural prediction and comparison revealed JcLIP protein with the right structural core and catalytic activity site of lipase. The random coils and insertions of the JcLIP protein sequence is partially different in structure from that of lipase of Rhizomucor miehei, which may imply a different activity.
    结构预测和比较表明,JcLIP蛋白质具有脂酶的结构核心和催化活性中心,而在非核心区具有较毛霉脂酶更多的插入和随机卷曲,这可能是决定二者之间酶活差异的重要原因。
短句来源
  “activity site”译为未确定词的双语例句
    The protein ACE2A (65KD) but not ACE2B (77KD) could bind to S1 protein of SARS-CoV. This result showed that the receptor activity of ACE2 was not related to the enzyme activity site, and the receptor functional domain was within N terminal 335 amino acids. This was so far the shortest ACE2 fragment expressed which had SARS-CoV receptor function.
    将包涵体变性、复性后,经Western blot证实pGEX-ACE2A表达的蛋白(~65KD)能与SARS-CoV S1蛋白特异结合,而pGEX-ACE2B表达的蛋白(~77KD)不能与S1蛋白结合。
短句来源
    The low affinity between P12 and DEP-KM may be caused by the loss of a basic amino acid in this surface activity site.
    应用GST Pull-down结合实验证明,GST融合的12肽在细胞内与DEP存在直接相互作用,其与DEP-KM的结合力则要比DEP弱很多。
短句来源
    The peptide was found to possess the conserved sequence motif Asp-Xaa-Gly, which was the enzyme activity site of microbial acidic protease. pPIC9K-ap was constructed successfully and subsequently electrotransformed into P. pastoris GS115. Initial fermation experiments were carried out and methanol was used as sole carbon source.
    通过PCR扩增出N. crassa酸性蛋白酶结构基因,将其插入到带有酵母信号肽α因子的表达载体pPIC9K的EcoR Ⅰ位点,正向连接的重组质粒pPIC9K-ap经Nco Ⅰ酶切纯化后,电转化入巴斯德毕赤氏酵母GS115。
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  activity site
RM1P was antigenic and reacted with polyclonal sera and 5 monoclonal antibodies (MAbs) spanning 4 epitopes including the membrane binding site and the transcription inhibition activity site.
      
These studies allow a tentative assignment of the cofactor activity site to the residues 177-322 of the 549 amino acid long chain of C4b-binding protein but indicated that residues in the region 332-395 are important in the binding activity.
      
The two non-responding experiments were conducted on a wall-drained, low-activity site (ARA means ranging 17-68 n moles ethylene core-1 h-1), the responding experiment was conducted on a poorly drained, high-ARA site.
      
MB+ and BNAH were separately accommodated within the β-CD cavity and the cavity walls may protect the activity site of the reactants.
      
Multivariate analysis classified the sampling sites in four groups based on the metal content in vegetal leaves in agreement with traffic and human activity site.
      
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Objective:To predict the secondary structure and B cell epitope of human decay accelerating factor Methods:The accuracy of two secondary structure prediction methods,Chou & Fasman and PHDsec,was compared,and then human DAF secondary structure was analyzed by PHDsec Hydrophilicity scores and solvent accessibility of DAF SCR1 4 were obtained by methods of Hopp & Woods and PHDacc respectively Combined with the secondary structure of DAF,the B cell epitopes in DAF were predicted Results:PHDsec which use...

Objective:To predict the secondary structure and B cell epitope of human decay accelerating factor Methods:The accuracy of two secondary structure prediction methods,Chou & Fasman and PHDsec,was compared,and then human DAF secondary structure was analyzed by PHDsec Hydrophilicity scores and solvent accessibility of DAF SCR1 4 were obtained by methods of Hopp & Woods and PHDacc respectively Combined with the secondary structure of DAF,the B cell epitopes in DAF were predicted Results:PHDsec which use multiple sequence alignment,achieve a better prediction accuracy There is no alpha helix in SCR1 4 of human DAF but only beta sheet and loop segments; The computer predicted most possible epitope in DAF localize within amino acid residues Pro73 Val79?Arg130 Leu139 and Glu156 Cys163 Conclusion:These results will be useful in estimate of the epitope and activity sites localized within human DAF

目的 :分析预测人衰变加速因子的二级结构特征及B细胞表位。方法 :比较Chou&Fasman经典方案和基于多重序列比较的PHDsec二级结构预测方案的预测准确率 ,应用较优方案对DAF的二级结构进行预测分析 ;运用Hopp&Woods的亲水性方案及PHDacc可及性方案预测DAF的亲水性和可及性 ,结合DAF的二级结构特征 ,分析预测DAF的B细胞表位。结果 :PHDsec方案对SCR的预测准确率明显高于Chou&Fasman方案 ,DAF的SCR1 4中无α螺旋 ,仅包含 β折叠及袢 ;推测最可能的抗原表位位于Pro73 Val79、Arg130 Leu139及Glu15 6 Cys16 3;SCR1、SCR2与SCR3、SCR4在亲水性及二级结构分布方面具有较大差异。结论 :研究的分析预测结果将有助于确定DAF的B细胞表位及其生物学活性部位

Objective To study structure and function of IFN-α2a, IFN-α2b and IFN-α1b. Methods 6 mAbs were used in antiproliferative activity, antiviral activity and ELISA assays. Results Results showed that mAbs affecting antiviral activity also affected antiproliferative activity while mAbs that affecting antiproliferative activity did not necessarily affect antiviral activity. ELISA results showed that the site binding cellular receptor was different from which binding mAbs. Conclusions Antiviral activity site...

Objective To study structure and function of IFN-α2a, IFN-α2b and IFN-α1b. Methods 6 mAbs were used in antiproliferative activity, antiviral activity and ELISA assays. Results Results showed that mAbs affecting antiviral activity also affected antiproliferative activity while mAbs that affecting antiproliferative activity did not necessarily affect antiviral activity. ELISA results showed that the site binding cellular receptor was different from which binding mAbs. Conclusions Antiviral activity site of IFN was that of its antiproliferative activity, though some other sites might also be involved in the functioning of antiviral activity. The neutralizing activity of mAbs was not formed through preventing the binding of IFN molecules to cellular receptor.

目的 利用自制的 6株单克隆抗体对α2a 干扰素、α2b 干扰素和α1b 干扰素的结构与功能进行分析。方法 采用细胞ELISA法、抗增殖活性和抗病毒活性等试验。结果 对干扰素抗病毒活性有影响的单克隆抗体对干扰素抗增殖活性也有影响 ,而对干扰素抗增殖活性有影响的 ,不一定对干扰素抗病毒活性有影响。结论 干扰素表面细胞受体结合位点与单克隆抗体结合位点是分开的。干扰素的抗病毒活性位点也是它的抗增殖活性位点 ,而干扰素的抗增殖活性作用的发挥可能还有其它位点的参与 ;单克隆抗体的中和活性不是通过阻断干扰素分子与细胞表面受体结合造成的。

The effect of dimethyl sulfxide(DMSO) on the activity of Haliotis diversicolor alkaline phosphatase(ALP) was studied.The results showed that the enzyme activity rapidly declined with increasing DMSO concentrations.The inactivation of the enzyme in DMSO solutions was reversible reaction.The inhibition of DMSO on the enzyme was found to be mixed type and the presence of substrate had protection on the enzyme inhibited by DMSO.The conformational changes of the enzyme in different concentrations of DMSO solution...

The effect of dimethyl sulfxide(DMSO) on the activity of Haliotis diversicolor alkaline phosphatase(ALP) was studied.The results showed that the enzyme activity rapidly declined with increasing DMSO concentrations.The inactivation of the enzyme in DMSO solutions was reversible reaction.The inhibition of DMSO on the enzyme was found to be mixed type and the presence of substrate had protection on the enzyme inhibited by DMSO.The conformational changes of the enzyme in different concentrations of DMSO solution were measured by fluorescence absorption spectra.The results showed that the fluorescence emission peak in intensity of the enzyme gradually strengthened with increasing DMSO concentrations.Those results were in concordance with the conformational changes of the microenvironments of tyrosine and tryptophan residues of the enzyme.It can be recognized that conformational integrity is important for maintaining the activity of the enzyme and slight changes at the activity site could lead to inactivation of the enzyme.

以二甲亚砜(DMSO)为效应物,研究其对杂色鲍碱性磷酸酶(ALP)活力的影响,结果表明:该酶的剩余活力随着DMSO浓度增大而迅速下降,当DMSO浓度达40%,酶活力几乎完全丧失.说明DMSO对杂色鲍ALP有明显的失活作用.导致酶活力丧失50%的DMSO浓度为17%.在较低DMSO浓度(<20%)的失活是可逆的反应过程.动力学研究表明,该酶的失活过程属于混合型.进一步测定游离酶(E)和酶底物络合物(ES)与DMSO的结合常数(KI和KIS),结果表明KI

 
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