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genetically modified component
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  “genetically modified component”译为未确定词的双语例句
     Detection of genetically modified component in familiar edible fungi by multiplex PCR
     常见食用菌中转基因成分的多重PCR检测技术模拟
短句来源
     The results showed the multiplex PCR was quick,effective,simple and exact,and might play an important role in the detection of genetically modified component.
     并以大豆、水稻等农作物样品为检测材料,采用单一PCR和多重PCR技术同时进行检测,结果表明多重PCR方法具有快速、高效、简便、准确等特点,在转基因成分的检测上具有非常重要的实际应用价值和潜力。
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  相似匹配句对
     Genetically modified food
     谁来捅破转基因这层窗户纸
短句来源
     Detection of Genetically Modified Food
     转基因食品的检测
短句来源
     of modified P.R.
     R.
短句来源
     Component Update
     元器件快览
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     NET component, .
     如.NET组件开发技术,.
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The total DNAs of maize and its products were extracted by CTAB method. The positive samples were screened by detecting of several genetically modified components such as Cauliflower mosaic virus(CaMV) 35S promoter, Agrobacterium tumefaciens nopaline synthase(nos) terminator, A.tumefaciens strain CP4 EPSPS gene, Treptomyces hygroscopicus bar gene and Bacillus thuringiensis subsp. kurstaki cryIA(b) gene with single PCR. The multiplex PCR methods detecting synchronously of maize endogenous zein gene and...

The total DNAs of maize and its products were extracted by CTAB method. The positive samples were screened by detecting of several genetically modified components such as Cauliflower mosaic virus(CaMV) 35S promoter, Agrobacterium tumefaciens nopaline synthase(nos) terminator, A.tumefaciens strain CP4 EPSPS gene, Treptomyces hygroscopicus bar gene and Bacillus thuringiensis subsp. kurstaki cryIA(b) gene with single PCR. The multiplex PCR methods detecting synchronously of maize endogenous zein gene and several genetically modified components were developed. The results showed that the multiplex PCR methods developed for detecting synchronously of endogenous gene and genetically modified components were practical and worthy of being popularized, and there is no commercial genetically modified maize in our country, but foreign commercial transgenic maizes were inpoured into fujian province.

采用CTAB法提取玉米及其制品的总DNA,用PCR方法检测其中的转基因成分如花椰菜花叶病毒(Cauliflowermosaicvirus,CaMV)35S启动子、根癌农杆菌(Agrobacteriumtumefaciens)胭脂碱合成酶基因(nos)终止子、根癌农杆菌CP4菌株的EPSPS基因、吸水链霉菌(Treptomyceshygroscopicus)bar基因及苏云金芽孢杆菌库尔斯塔克亚种(Bacillusthuringiensissubsp.kurstaki)cryIA(b)基因,筛选到阳性样品,并建立了几组玉米内源zein基因和转基因成分之间的多重PCR检测方法。结果表明,建立的多重PCR方法用于同时检测玉米内源基因和转基因成分是可行的,值得推广;虽然我国还未有已获准商品化生产的转基因玉米,但国外转基因玉米已流入福建省。

The genetically modified ingredients introduced including promoter,terminator,selectable marker genes and structural genes(encoding the novel protein) etc were detected with the multiplex PCR technology in order to establish a quick,exact and effective technical system of the screening of genetically modified crops.The triplex PCR of soya inner LECTIN gene,Cauliflower mosaic virus(CaMV)35S promoter and Agro-bacterium tum ef aciens nopaline synthase(NOS) terminator,and the triplex PCR of CaMV35S promoter,NPTII...

The genetically modified ingredients introduced including promoter,terminator,selectable marker genes and structural genes(encoding the novel protein) etc were detected with the multiplex PCR technology in order to establish a quick,exact and effective technical system of the screening of genetically modified crops.The triplex PCR of soya inner LECTIN gene,Cauliflower mosaic virus(CaMV)35S promoter and Agro-bacterium tum ef aciens nopaline synthase(NOS) terminator,and the triplex PCR of CaMV35S promoter,NPTII and HPT genes were successfully experimented.The transgenic soya and rice samples were tested with the multiplex and simplex PCR technique,respectively.The results showed the multiplex PCR was quick,effective,simple and exact,and might play an important role in the detection of genetically modified component.

以转基因大豆、水稻等样品为材料,采用多重PCR技术对转基因作物中常见的启动子、终止子、筛选标记基因和转入的目的基因等多个外源转基因元件进行检测,以期建立一套快速、准确、高效的转基因农作物筛查鉴定技术。通过多重扩增实验,建立了关于转基因大豆检测的大豆内源Lectin基因,花椰菜花叶病毒(Cauliflower mosaic virus,CaMV)35S启动子和根癌农杆菌胭脂碱合成酶基因NOS终止子的三重PCR分析体系,及关于转基因水稻检测的CaMV35S、NPTII和HPT基因的三重PCR分析的技术体系。并以大豆、水稻等农作物样品为检测材料,采用单一PCR和多重PCR技术同时进行检测,结果表明多重PCR方法具有快速、高效、简便、准确等特点,在转基因成分的检测上具有非常重要的实际应用价值和潜力。

In order to developing duplex PCR method which could detect synchronously several genetically modified components, the reaction system and condition of the duplex PCR were optimized using transgenic Roundup Ready soybean as experimental material, including the concentration of primers, units of Taq DNA polymase and anneal temperature. The sensitivity and detection content of duplex PCR were compared with those of single PCR. The results showed that sensitivity and detection content of duplex PCR optimized...

In order to developing duplex PCR method which could detect synchronously several genetically modified components, the reaction system and condition of the duplex PCR were optimized using transgenic Roundup Ready soybean as experimental material, including the concentration of primers, units of Taq DNA polymase and anneal temperature. The sensitivity and detection content of duplex PCR were compared with those of single PCR. The results showed that sensitivity and detection content of duplex PCR optimized were as the same as those of single PCR. The positive samples with genetically modified components were screened successfully from soybean, bean curb, soya residue and fried bean curb by the duplex PCR developed. The reagent and time needed for duplex PCR were less than those of sinlge PCR. Therefore,the duplex PCR might be popularized to detect genetically modified products.

为了建立能同时检测多种转基因成分的二重PCR检测方法,以转基因大豆(RoundupReady品种)为实验材料,优化了二重PCR的反应体系和反应条件,包括引物浓度、TaqDNA酶用量和退火温度等;比较了二重PCR和单一PCR的灵敏度和检测含量。结果表明:二重PCR的灵敏度和检测含量与单一PCR的相当;用建立的二重PCR方法从大豆、豆腐、豆粕和油炸豆腐中筛选出含转基因成分的阳性样品;二重PCR与单一PCR相比,具有节约试剂、省时等特点,在转基因产品检测上具有很好的推广价值。

 
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