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recombinant toxin
相关语句
  重组毒素
     Expression of recombinant toxin IL6_(23)-PE40 and its cancer cell-killing effect
     IL6_(23)-PE40重组毒素的表达和对肿瘤细胞的杀伤效果
短句来源
     The design and prediction of biology characteristics of recombinant toxin CD80-Linker-SEA
     人CD80-Linker-SEA重组毒素的设计及生物学特性预测
短句来源
     Experimental Study on Construction of Recombinant toxin MSH-PE40 and its anti-tumor activity
     MSH-PE40重组毒素的构建和抗头颈部肿瘤的实验性研究
短句来源
     Objective To obtain the target cells expressing HIV-1 gp160 membrane protein used for the determination of killing activities of HTV-1 specific cytotoxic T lymphocytes and anti-HTV-1 recombinant toxin cells.
     目的获得表达INV-1 gp160膜蛋白的靶细胞,用于HIV-1特异性细胞毒性淋巴细胞(CTL)和抗HIV-1重组毒素细胞杀伤活性检测。
短句来源
     Cytotoxity of EGF-PE4O recombinant toxin on Hep-2 cell line of laryngeal squamous cell cancer
     EGF-PE4O重组毒素对喉鳞癌细胞株Hep-2的毒性作用
短句来源
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  “recombinant toxin”译为未确定词的双语例句
     Preliminary Study on Fermentation Conditions of the Recombinant Toxin LHRH-PE40in E.Coli.
     LHRH-PE40工程菌发酵条件的初步研究
短句来源
     Conclu sion:The stable and efficient expression of the recombinant toxin B7-2 -PE40KDEL mainly in soluble form was obtained,which lays a foundation, for the purification of B7-2-PE40KDEL.
     结论:通过优化各种已知影响原核表达系统的相关因素,获得了稳定、高效的几乎全部以可溶性形式表达的重组B7-2-PE40KDEL融合蛋白的工程菌,为进一步有效分离纯化该蛋白奠定了基础。
短句来源
     Construction of recombinant toxin MSH-Ang
     重组表达质粒MSH-Ang的构建
短句来源
     Expression and purification of recombinant toxin MSH-Ang
     重组蛋白MSH-Ang的构建表达和纯化分析
短句来源
     The recombinant plasmid was transformed into E. coli DH5α and recombinant toxin Hc-VT1B-SEA-VT2B-SEB,terming F5,gene was constructed. Uising DNAstar,the characteristics of recombinant toxin,such as antigenicity,were predicted by computer analysis.
     方法:采用一定的linker序列(G-P-G-P-G)将克隆的3种食物中毒菌5个毒素基因片段进行串联,构建重组融合毒素Hc-VT1B-SEA-VT2B-SEB(命名为F5)基因,应用DNAstar,对F5融合蛋白抗原特性等进行预测分析。
短句来源
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  相似匹配句对
     Recombinant E.
     分别以卡拉胶、明胶、海藻酸钠包埋 E.
短句来源
     Construction of recombinant toxin MSH-Ang
     重组表达质粒MSH-Ang的构建
短句来源
     Purification of Recombinant Aer Toxin and Antigenicity Analysis
     嗜水气单胞菌重组Aer毒素的纯化和抗原性分析
短句来源
     2.toxin.
     2疫毒学说;
短句来源
     The recombinant plasmid of E.
     应用细菌质粒转化技术,将大肠杆菌K_(?)
短句来源
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  recombinant toxin
Apoptosis was also induced by the recombinant toxin B subunit alone, suggesting that the ligation of Gb3 is the primary induction mechanism.
      
OLX-209 is a recombinant toxin targeting theerbB-2 oncoprotein.
      
Preclinical testing of an anti-erbB-2 recombinant toxin
      
The levels of recombinant toxin were established and biological activity tests were performed against neonate sugarcane borer (Diatraea saccharalis F.) larvae.
      
Bioassays were carried out to verify the toxicity of this recombinant toxin.
      
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Inorder to find a new kind of biological agent for the treatment of headand neck aquamous cell cancer,cytotoxic activity of recombinant toxin EGF-PE40(epi-dermal growth factor-pseudomonas exotoxins-40) on laryngeal squamous cancer cellswere observed. The biologic activity of EGF-PE40 recombinant toxin was measured byMTT colorimeric assay and Hep-2 cells were observed under light microscope. This as-say determined the concentration of Hep-2 cells to be 2 × 1O4 cells/well, selected lO%SDS-0. 01m0l/L...

Inorder to find a new kind of biological agent for the treatment of headand neck aquamous cell cancer,cytotoxic activity of recombinant toxin EGF-PE40(epi-dermal growth factor-pseudomonas exotoxins-40) on laryngeal squamous cancer cellswere observed. The biologic activity of EGF-PE40 recombinant toxin was measured byMTT colorimeric assay and Hep-2 cells were observed under light microscope. This as-say determined the concentration of Hep-2 cells to be 2 × 1O4 cells/well, selected lO%SDS-0. 01m0l/L HCL as s0lubilizers of MTT formazan. Cytotoxcity reached 50% withEGF-PE40 recombinant toxin at 0. 53ng/ml. Cell damage became more serious with re-combinant toxin increasing in a certain range.

观察EGF-PE40重组毒素对喉鳞癌细胞的毒性作用,为寻找新型的治疗头颈鳞癌的生物制剂奠定基础。应用MTT比色法测定EGF-PE40重组毒素的生物学活性。并在光镜下观察Hep-2细胞形态变化。确定MTT比色法中Hep-2细胞浓度为2×104/well。选择10%SDS-0.01mol/LHCI作为MTT结晶的溶解液,当EGF-PE40重组毒素为0.53ng/ml时,50%的Hep-2细胞死亡,在一定范围内,随重组毒素增加,细胞损伤加重。

Angiogenin cDNA amplified by PCR and PE40 cDNA was inserted into pUC19.Angiogenin PE40 recombinant toxin was constructed which was confirmed by DNA sequencing.The fusion gene was then cloned into the expression vector pRSETB.After being tranformed into E.coli BL21DE3(pLysS) host bacteria,the recombinant protein was expressed in inclusion bodies with the yield of 8% total bacteria proteins.The recombinant toxin's molecular weight was about 58 kD.The inclusion bodies were dissolved in 8...

Angiogenin cDNA amplified by PCR and PE40 cDNA was inserted into pUC19.Angiogenin PE40 recombinant toxin was constructed which was confirmed by DNA sequencing.The fusion gene was then cloned into the expression vector pRSETB.After being tranformed into E.coli BL21DE3(pLysS) host bacteria,the recombinant protein was expressed in inclusion bodies with the yield of 8% total bacteria proteins.The recombinant toxin's molecular weight was about 58 kD.The inclusion bodies were dissolved in 8 mol/L urea and purified by affinity chromatography using Ni 2+ NTA resin.The recombinant toxin showed a single band on SDS PAGE.The activity CAM assay showed that the purified protein could inhibit the formation of novel blood vessel effectively.

利用 PCR扩增出人血管生成素 (h ANG)成熟肽的基因片段 .与绿脓杆菌外毒素缺失突变体PE40的基因连接后 ,克隆入 p UC1 9载体中 .测序后克隆入表达载体 p RSETB,构建成 h ANG-PE40融合基因的表达载体 .IPTG诱导 ,表达出分子量约为 58k D的 His6- ANG- PE40融合蛋白 ,占菌体总蛋白的 8% .Ni2 +- NTA树脂纯化表达蛋白 ,SDS- PAGE结果显示纯化重组蛋白为单一条带 .鸡胚绒毛尿囊膜鉴定表明重组蛋白体外能够有效地抑制血管的形成 .

Objective:To construct the eukaryotic expressed vector which express recombinant toxin CD80 Linker SEA and predict the rationality and feasibility of the linker Methods:Utilize the sequence analysis software to analyze the flexibility、antigenicity、Hoop&Woods hydrophilicity and episode of recombinant toxin CD80 Linker SEA Results:Through the analysis of the software,it could be found that the recombinant toxin has correct domains of CD80 and SEA The linker has low episode、low antigenicity...

Objective:To construct the eukaryotic expressed vector which express recombinant toxin CD80 Linker SEA and predict the rationality and feasibility of the linker Methods:Utilize the sequence analysis software to analyze the flexibility、antigenicity、Hoop&Woods hydrophilicity and episode of recombinant toxin CD80 Linker SEA Results:Through the analysis of the software,it could be found that the recombinant toxin has correct domains of CD80 and SEA The linker has low episode、low antigenicity and high flexibility Conclusion:The results of computer analysis could help us to rationally design the recombinant toxin CD80 Linker SEA and keep it's maximum biological activity

目的 :构建人重组CD80 Linker SEA毒素的真核表达载体并预测Linker的合理性和可行性。方法 :应用核酸和蛋白质序列分析软件GolgKey对融合基因及Linker部位翻译后在二级结构水平上的一些生物学特性 ,诸如柔性、抗原性、亲水性及表位等加以预测。结果 :CD80 Linker SEA融合基因的氨基酸序列经软件分析 ,并分别与CD80和SEA单独的分析结果相比较 ,发现在二级结构的水平未出现新的抗原性 ,同时在Linker部位具有很低的抗原性 ,亲水性没有改变 ,Linker部位呈中性 ,CD80和SEA具有原来各自的表位特征 ,无新的表位出现。结论 :本研究通过计算机软件对CD80 Linker SEA融合蛋白的预测 ,并与CD80、SEA各加以对比分析和比较 ,以利于在重组过程中有一个合理的设计 ,有可能最大程度的保留CD80和SEA各自的生物活性和功能

 
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