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限制性酶谱
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  restriction pattern
     Methods:Using the probe of 16S rRNA gene,we investigated the 16S rRNA gene restriction pattern(16S rRNA GRP) of 88 strains of Vibrio cholerae after their chromosomes were digested with BglⅠ. M-PCR was used to analyze six toxigenes(ctxA,rtxA,Ace,TcpA,Cri and Zot) in 140 strains of Vibrio cholerae.
     方法:利用16S rRNA基因探针分析了浙江省不同时期分离的88株霍乱弧菌经BglⅠ消化的16S rRNA基因限制性酶谱,以多重PCR法对140株霍乱弧菌进行6种毒力相关基因(ctxA、rtxA、Ace、TcpA、Cri、Zot)检测分析。
短句来源
  restriction mapping
     By the blue/white screening,a recombinant plasmid with ADH1C gene was selected and identified by restriction mapping and DNA sequencing analyses.
     经过蓝白筛选后,用限制性酶谱和DNA测序以及BLAST对比分析进行鉴定。
短句来源
     ③The identified results of restriction mapping,DNA sequencing and sequence alignment analysis by BLAST proved that recombinant pUC19-ADH1C*1 plasmid was constructed successfully.
     ③用限制性酶谱和DNA测序并作BLAST分析证实pUC19-ADH1C*1重组质粒构建成功。
短句来源
  “限制性酶谱”译为未确定词的双语例句
     The genomic DNA extracted from a new virulence 21C3CTR (p11) and common virulent types: races 21C3, 34 and 34C2 and pathotypes 21C3CKH, 34MKH, 34MKG and 34C2MKR of Puccinina graminis f.
     用3种DNA限制性内切酶(EcoRⅠ,PstⅠ和HindⅢ),分析了中国小麦秆锈菌新致病型21C3CTR(p11),普通致病型的小种21C3,34,34C2和致病型21C3CKH,34MKH,34MKG,34C2MKR的限制性酶谱
短句来源
     Inordertoresearchtherelationshipbetweenmyelodysplasticsyndromes(MDS)cloneevolutionandmicrosateliteinstability(MSI)orlossofheterozygosity(LOH)ofdeletedcolorectalcancer(DCC)gene,themicrosatelite(TA)26N28(AT)8andpolymorphiclocusD18S8ofDCCgenewereassayedbyPCRandrestrictionmapanalysisin17casesofMDS.
     为研究骨髓增生异常综合征(MDS)病情进展中是否发生基因改变,用PCR及限制性酶谱分析17例MDS患者骨髓DNA的DCC抗癌基因中的微卫星(TA)26N28(AT)8序列和多态性D18S8位点,探索基因的微卫星序列的不稳定性和杂合性缺失(LOH)与MDS骨髓异常细胞克隆演化的关系。
短句来源
     The enzyme incision pattern of rDNA-ITS of M.paraplatani was similar to that of M.arenaria by TaqⅠ、RsaⅠ、MspⅠ、ClaⅠ,but different from that of M.arenaria by HaeⅢ.
     TaqⅠ、RsaⅠ、MspⅠ、ClaⅠ酶切拟悬铃木根结线虫的rDNAITS扩增产物所获得限制性酶谱与花生根结线虫的限制性酶谱一致,只有HaeⅢ酶切拟悬铃木根结线虫和花生根结线虫的rDNAITS产物所得酶谱不同。
短句来源
     Results:The coding sequence of bax and pSV-CIP-bax-CAT gene was characterized by restriction analysis.
     结果 :bax基因编码序列及嵌合基因限制性酶谱正确 ;
短句来源
     Methods By the method of restriction enzyme mapping, we investigated the allele frequencies of T235 and M235 and the proportions of the three genotype MM, TM and TT of the polymorphism of angiotensinogen gene in the group of normal controls (n=68). diabetic normotension (n = 104) and diabetic hypertension (n = 87).
     方法用限制性酶谱分析法检查AGT基因M235T多态性M235和T235的频率和TT、TM和MM基因型在正常对照组(68例)、糖尿病非高血压组(104例)和糖尿病高血压组(87例)中的分布,并检查各基因型对实验对象血压水平的影响。
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  相似匹配句对
     Restriction enzyme pattern analysis of Mycobacteria genosome
     分枝杆菌属基因限制性内切酶谱分析
短句来源
     COMPARISON OF RESTRICTION ENDONUCLEASE FINGERPRINTING OF DNA FROM SEVERAL STRAINS OF TUBERCULOSIS BACILLUS
     几株结核杆菌DNA的限制性内切酶谱比较
短句来源
     Limited Proxy Digital Signature Schemes
     限制性代理签名方案
短句来源
     The results also show that the relations of E.
     从POD酶谱上分析,E.
短句来源
     Discussing the Restrictive Character of Social Sciences Function
     略论社会科学作用的限制性
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  restriction pattern
Some phages only slightly differing in DNA restriction pattern from ?KZ may be used to study the origin of ?KZ genes coding for orthologs of proteins of unrelated species (other phages, pathogenic bacteria, eukaryotes).
      
11 of 64 clones from the immunized animal had consistent restriction pattern, while none of the 64 clones from the non-immunized animal had, except that one had the same fragments pattern as that of the 11 clones.
      
Two patients with encephalomyopathy and two with the MERRF syndrome (myoclonus epilepsy with ragged red fibres) had the normal PvuII restriction pattern of muscle mitchondrial DNA.
      
Normal restriction pattern (Hind III) of the myoadenylate deaminase gene in enzyme deficient patients
      
knightiana = NK) in the plants was determined on the basis of the species specific EcoRI restriction pattern of the chloroplast DNA.
      
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  restriction mapping
Their tandem arrangement was revealed by means of the PCR analysis of genomic DNAs of four Microtus species and by restriction mapping of clones selected from a M.
      
pZeoSV(+) ANN II was analyzed by restriction mapping and the Ann- II sequence identified.
      
Like restriction mapping, end-probing reveals the distance between restriction sites on a plasmid.
      
In contrast to restriction mapping, end-probing unambiguously reveals the order of those restriction sites.
      
Genotypes were determined by polymerase chain reaction (PCR) and restriction mapping.
      
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Two new analog genes of the α-human atrial natriuretic polypetide (α-hANP) were constructed by accurate intragenic recombination of analog RH-1 gene with ANP gene in vitro, both of which had been synthesized and reported. One recombined analog gene, RH-2, was designed to contain an equivalent of the a-hANP gene, capping the peptidase inhibitor SQ20881 sequence at its 5'end. The other analog gene, RH-3, was designed to contain an equivalent of the a-hANP gene,wedging in two proline codons CCG & CCG at the 3'...

Two new analog genes of the α-human atrial natriuretic polypetide (α-hANP) were constructed by accurate intragenic recombination of analog RH-1 gene with ANP gene in vitro, both of which had been synthesized and reported. One recombined analog gene, RH-2, was designed to contain an equivalent of the a-hANP gene, capping the peptidase inhibitor SQ20881 sequence at its 5'end. The other analog gene, RH-3, was designed to contain an equivalent of the a-hANP gene,wedging in two proline codons CCG & CCG at the 3' end. The recombinants bearing the genes, bacteriophage RH-2/M13mpl8 & RH-3/ M13mpl8, were identified by DNA domain combination analysis- in situ hybridization and dot blotting with three probec matching three specific DNA domains, and by restriction analysis. The DNA sequences of RH-2 and RH-3 were confirmed by the dideoxynucleotide chain termination method.

以精确设计的基因内重组法,利用已有的心钠素衍生物RH—1基因和心钠素α-hANP基因的重组交换,设计并构建了两种新的心钠素衍生物RH-2和RH-3基因。衍生物RH-2相当于α-hANP的N端嵌合羧肽酶抑制剂SQ20881,衍生物RH-3相当于α—hANP的C端嵌合两个脯氨酸。基因内重组获得的重组体用三种探针以原位杂交和斑点杂交等方法进行DNA区域组合分析,再经限制性酶谱分析筛选出目的克隆。DNA序列测定确认了所构建得的RH-2和RH-3基因。

Since October of 1992,large epidemics of cholera-like

1992年10月在印度,12月开始在孟加拉国发生了一种新型霍乱弧菌——霍乱弧菌O139引起的霍乱样大流行。我国新疆南部柯坪县于1993年5~6月也发生了由该型菌引起的典型霍乱样腹泻暴发。分子生物学研究表明:细菌染色体DNA经核酸限制性内切酶SalⅠ消化后,用核酸脉冲场凝胶电泳(PFGE)分离DNA,所有O139菌株表现为一致的特征性的限制性酶谱。用16SrRNA基因为探针,染色体DNA经BglⅠ酶切PFGE分离后做southernblot杂交分析,结果印度、孟加拉国和中国分离的菌株分别属于不同的核糖体基因型(Ribotype,RT),但与新疆菌株相比,印度和孟加拉国菌株的关系更为密切。结果还显示:霍乱弧菌O139菌株具有基本一致的基因组特征。

his paper reported theresults of molecular biological study on 2 1 strains of vibriostatic agent O/129-resistant vibrio cholerae non-O1 isolated from patients of acute diarrhoea admitted to Beijing TianTan Hospital from May 1992 to Octorber 1994.The results showed that the restriction patterns of chro-mosomal DNA of the strains were diverse after the chromosomal DNAs being digested with Pst 1 andseparated by agarose gel electrophores. By southern blot hybridization analysis with gene probe labelledwith Dig,non...

his paper reported theresults of molecular biological study on 2 1 strains of vibriostatic agent O/129-resistant vibrio cholerae non-O1 isolated from patients of acute diarrhoea admitted to Beijing TianTan Hospital from May 1992 to Octorber 1994.The results showed that the restriction patterns of chro-mosomal DNA of the strains were diverse after the chromosomal DNAs being digested with Pst 1 andseparated by agarose gel electrophores. By southern blot hybridization analysis with gene probe labelledwith Dig,non of the strains possessed the fragments of CT as the control strain of the vibro choleraeO1.The examination for the presence of plasmid DNA showed that plasmid bands were not detected inthe O/129-resistant strains except 6 strains which had the presence of one plasmid and were also foundto be resistant to 3-8 antibiotics commonly used in clinical practices. Thus O/129 resistance is not to beplasmid mediated in the strains tested.The results demonstrated that O/129-resistant vibrio choleraenon-O1 were similar to those non-epidemic strains of vibrio cholerae O1 on genotype and toxin genescharacteristic. It is concluded that O/129-resistant vibrio cholerae non-O1 were not to be the pathogenof vibrio cholerae outbreak.

为了从遗传学基础上,探索抗O/129的非O1群霍乱弧菌的基因结构的特点,即对1992~1994年从肠道门诊急性腹泻患者粪便中分离到的21株抗O/129的非O1群霍乱弧菌的分子生物学特性进行了研究。结果显示:染色体DNA经核酸限制性内切酶PstI消化后,用琼脂糖凝胶电泳分离DNA,全部菌株表现为多样化特征的限制性酶谱;用Dig标记的基因探针做southernblot杂交,无一株菌具有O1群霍乱弧菌流行株所具有的CT毒素基因;质粒图谱分析,仅有6株菌各含有一条质粒带,而且这6株菌同时对3~8种临床常用抗生素耐药,表明该菌株对O/129的抗性是非质粒性的。结果表明,抗O/129的非O1群霍乱弧菌与O1群霍乱弧菌非流行株具相似的遗传学特性和毒力特征,因而,它不是引起霍乱暴发的病原体。

 
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