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mice
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  小鼠
    The suppressive effect of anti-idiotypic antibodies induction on transplantation immunoreaction in mice
    抗独特型抗体诱导对小鼠移植免疫反应的抑制作用
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    Study on the Impact of Hot-spot Mutations in HBV/C Gene on the Immune Response of Mice by Gene Transfer Mediated by Recombinant Adenovirus
    用腺病毒载体介导的基因转移研究HBV/C区热点变异对小鼠免疫应答的影响
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    Inducing Immune Response to Apical Membrane Antigen-1 by DNA-based Combined Vaccination in Mice
    以DNA为基础的复合免疫接种诱导小鼠抗AMA1免疫应答
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    Induction of Tolerance in MHC Disparate Allo-geneic Mice Bone Marrow Transplantation by Combination of Tju103 and CTLA4-Ig
    Tju103和CTLA4Ig联合诱导MHC非匹配同种异基因小鼠骨髓移植免疫耐受
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    Establishment and Analysis of Transgenic Mice Carrying the Gene of Human Nuclear Receptor NR5A2(hBlF)
    人核受体nr5a2(hblf)转基因小鼠的建立及其分析
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    The Estimation of Protective Efficacy of the Fusion Gene Vaccine Encoding Tubercle Antigen 85B and MPT64 in Mice Challenged with M.Tuberculosis
    结核分枝杆菌Ag85B-MPT64融合基因疫苗对结核杆菌感染的保护作用
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    The Study of Suppressed Mechanism of IL-12p40 Gene from PEC of Plasmodium Berghei-infected Mice
    疟原虫(Plasmodium bergher)感染PEC细胞IL-12p40基因表达抑制机制的研究
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    Gene Fusion of Molecular Adjuvant C3d3 to hCGβ Enhances Anti-hCGβ Humoral Response in DNA Immunization to Mice
    分子佐剂C3d3与hCGβ基因融合对DNA免疫增强抗hCGβ体液免疫效应
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    1. The Study of NOD.H-2~(h4) Mice T Cell Cloning 2. The Constructs of HPV, HSV Recombinated Prophylactic Vaccine
    1. NOD.H-2~(h4)T细胞克隆的研究 2. HPV,HSV基因工程预防疫苗的构建
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    Histoche mical Localization of Nonspecific Esterase in the Kidneys of Post Embryonic Period,Newborn and Adult Mice
    小胚胎后期、新生及成年肾非特异性酯酶的定位观察
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    The Construction of DNA Vaccine of Herpes Simplex Virus Type Ⅱ Glycoprotein D with Chemokine MIP-1α and Preliminary Immunity in Mice
    单纯疱疹病毒Ⅱ型gD糖蛋白加趋化因子MIP-1α核酸疫苗的构建及初步免疫观察
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    HCV C-FC Engineered Mice Dendritic Cells in Enhancing Immune Response Against HCV
    HCV C-Fc融合基因疫苗修饰的树突状细胞功能研究
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    Experimental Study on the Differentiation of Hepatocyte from Transplanted Human Umbilical Cord Blood Stem Cells in BALB/C Nude Mice
    脐血干细胞移植体内诱导分化为肝细胞的实验研究
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    DNA-liposome Vaccination with Genes Encoding Toxoplasma Gondii Antigens SAG1 and ROP1 Induces Partially Protective Immunity Against Lethal Challenge in Mice
    阳离子脂质体介导的弓形虫SAG1/ROP1复合基因的DNA免疫
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    Study on Coloning the Rhoptry Protein 1 Gene Related to Penetrating Factor from Toxoplasma Gondii and Its DNA Immunity in Mice
    弓形虫侵入相关分子ROP1基因的克隆及其DNA免疫研究
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  mice
Investigations of their biodistribution in mice showed all five pseudo-peptide chelators (MGQ, MGGQ, MAGQ, MVGQ, MFGQ) are rapidly cleared from blood, mainly through renal clearance.
      
The multiple antioxidant activity of the polysaccharides was evidenced by significant reducing power, superoxide scavenging ability, inhibition of mice erythrocyte hemolysis mediated by peroxyl free radicals, and also ferrous ion chelating potency.
      
Compounds Ia-Ih were hydrogenated with Pd-C to give IIa-IIh, and their hypoglycemic activity was evaluated with a glucose oxidase kit and insulin load test on normal mice.
      
The purified product was found to be biologically active and to reduce the food intake and body weight of mice during tests.
      
The effects of hematopoietic stem/progenitor cells (HSPCs) expanded in the two step coculture with human bone marrow mesenchymal stem cells (hMSCs) on the hematopoietic reconstruction of irradiated NOD/SCID mice were studied.
      
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The cells of the apical ectodermal thickening, neighboring ectoderm and underlyingmesenchyme of the limb buds of mouse embryos, 11--13 days vaginal plug age, wereused for this study. Most of the specimens were fixed in 1% osmic acid in veronalacetate buffer, pH 7.6, 4℃, 1 hour and embedded in methylchrylate. Parts of the speci-mens were either fixed in potassium permagnate or stained in lead hydroxide after osmicacid fixation. Observations and photographs were made under SEM Ⅲ electron micros-cope. The...

The cells of the apical ectodermal thickening, neighboring ectoderm and underlyingmesenchyme of the limb buds of mouse embryos, 11--13 days vaginal plug age, wereused for this study. Most of the specimens were fixed in 1% osmic acid in veronalacetate buffer, pH 7.6, 4℃, 1 hour and embedded in methylchrylate. Parts of the speci-mens were either fixed in potassium permagnate or stained in lead hydroxide after osmicacid fixation. Observations and photographs were made under SEM Ⅲ electron micros-cope. The Golgi apparatus appeared to be horse-shoe shaped in the apical ectodermalthickening and S-shaped in the neighboring ectoderm. It consisted of small vesicles andtubules without any large vesicle. The mitochondria in the neighboring ectoderm were usually round or oval in shape,with fewer and irregular cristae and lighter matrix. The mitochondria of the mesenchy-mal cells were even more embryonic in structure with only 2--3 oblique cristae and alarge inner chamber. The mitochondria in the supperficial layers of the apical ectoder-mal thickening were more or less similar to those in the neighboring ectoderm. Rod-shaped and filamentous mitochondria increased in number in the deeper layers of theapical thickening. Such filamentous mitochondria had vertical cristae, denser matrix andoval and light inner chambers. Transitional stages between the very small vesicles withvague cristae and the large mitochondria with distinct cristae were found in our leadstained preparations. Except in the Golgi region, ribosomes grouped into ring or tubular forms weredistributed all over the cytoplasm in the three kinds of the embryonic cells studied.Transitional stages between such ribosome clusters and the granulated vesicles and tubulescould be clearly demonstrated in the lead stained specimens. In the apical ectodermal thickening, granulated endoplasmic reticulum in the formof scattered small vesicles and tubules seemed to be more abundant than in the neighbor-ing ectoderm and underlying mesenchymal cells. But the most striking difference foundin the apical thickening was the expansion of the granulated endoplasmic reticula intolarge saccules, the cisternae of which containing a grayish dense substance. Another interesting phenomenon found in the apical ectodermal thickening was thepresence of dense bodies in the cytoplasm. They were in various sizes and densitieswith dense granules, masses or cords and various forms of vesicles. They might be round,oval or irregular with a complete, partial or entire absence of limiting membrane. Thosewithout membrane were usually irregular in form and could hardly be demarkated from their surrounding cytoplasm whence assembly of ribosome clusters, mitochondria andendoplasmic reticulum from the surrounding cytoplasm to form the dense bodies could befound. Preliminary histochemical studies found them to be positive in alkaline and acid-phosphatases and RNA staining. They were therefore considered to be lysosomes orcytosomes. The significance of the differences in mitochondrial form and structure, abundantand expanded cisternae of the granulated endoplasmic reticulum and the presence of thedense bodies in the apical ectodermal thickening in relation to embryonic differentiationof the limb was discussed.

用小白鼠胚胎阴栓日龄11—13天的前肢芽和后肢芽,锇酸固定,甲基丙烯酸甲酯及甲基丙烯酸丁酯包埋,在SEM Ⅲ型电子显微镜观察了肢芽尖端增厚外胚层、邻近普通外胚层及间充质细胞。部分材料用过锰酸钾固定或锇酸固定后氢氧化铅染色。各种细胞相互比较的结果如下: 1.两种外胚层的高尔基体极相似,均由小泡及小管组成,无大泡,横切时成为成群的小泡。普通外胚层细胞的高尔基体呈S形,增厚外胚层的马蹄形。 2.线粒体在普通外胚层卵圆形或圆形,嵴不整齐。增厚外胚层的线粒体在浅层细胞少,结构与普通外胚层相似,但愈至深层则细长的线粒体愈多。细长线粒体的嵴较密,亦较整齐,与表面垂直,基貭亦较致密。在尖端增厚外层细胞见到由胞质新形成的小泡过渡到小圆的线粒体。 3.无论在普通外胚层下或在尖端增厚外胚层下的间充质细胞,其线粒体的形状和结构相同,都是圆或卵圆,嵴少而靠近表面作半月状,内室大,基貭密度低,属比较原始的胚胎型。 4.嗜碱质在两种外胚层及间充貭细胞均以弥散的核朊粒为主,由5—10余粒组成小群落。这些群落在尖端增厚外胚层较大、较密,粒亦较多。普通外胚层细胞的有粒内貭网或动貭较间充貭为多,二者均系胚胎型,是分散的小泡或小管。尖端增厚外胚层的...

用小白鼠胚胎阴栓日龄11—13天的前肢芽和后肢芽,锇酸固定,甲基丙烯酸甲酯及甲基丙烯酸丁酯包埋,在SEM Ⅲ型电子显微镜观察了肢芽尖端增厚外胚层、邻近普通外胚层及间充质细胞。部分材料用过锰酸钾固定或锇酸固定后氢氧化铅染色。各种细胞相互比较的结果如下: 1.两种外胚层的高尔基体极相似,均由小泡及小管组成,无大泡,横切时成为成群的小泡。普通外胚层细胞的高尔基体呈S形,增厚外胚层的马蹄形。 2.线粒体在普通外胚层卵圆形或圆形,嵴不整齐。增厚外胚层的线粒体在浅层细胞少,结构与普通外胚层相似,但愈至深层则细长的线粒体愈多。细长线粒体的嵴较密,亦较整齐,与表面垂直,基貭亦较致密。在尖端增厚外层细胞见到由胞质新形成的小泡过渡到小圆的线粒体。 3.无论在普通外胚层下或在尖端增厚外胚层下的间充质细胞,其线粒体的形状和结构相同,都是圆或卵圆,嵴少而靠近表面作半月状,内室大,基貭密度低,属比较原始的胚胎型。 4.嗜碱质在两种外胚层及间充貭细胞均以弥散的核朊粒为主,由5—10余粒组成小群落。这些群落在尖端增厚外胚层较大、较密,粒亦较多。普通外胚层细胞的有粒内貭网或动貭较间充貭为多,二者均系胚胎型,是分散的小泡或小管。尖端增厚外胚层的动貭有独特形态,即池的一端扩张成大泡,内含网状致密物,显然含有较多的,要不是特异的蛋白貭。这种扩张动貭对于胚胎分化及诱导的关系曾加讨论。 5.动质的形成有证据表明是先从核朊粒群落出现膜成为动貭小泡或小管,再并合成较大的小泡和较长的小管。 6.增厚外胚层细胞的另一特点是比较普逼的存在着形状、大小及密度不同的致密体。有的完全致密,有的泡状,有的是二者的混合体,有的界限清楚,有的处于分散状态。在形态上类似溶酶体和卵子的多泡体。内含类似核朊粒的致密粒、退化线粒体及动质膜。这些致密体和细胞膜、核膜及高尔基体未显有何关系。基膜及细胞膜完好。未见增厚外胚层细胞有排出、吞食或饮液现象,亦未见致密体排至中胚层,故认为这些致密体起于胞貭。对于分化诱导关系曾提出讨论。 7.细胞核及核膜在三种细胞未见有显著不同。核膜有孔,其外膜可与动貭膜相连,核仁小,紧靠核膜,未见有排出现象。氢氧化铅染色的标本有时显示染色貭有微丝,这在胞貭核朊粒群落亦可出现。 8.细胞膜完整,比较直,膜的内侧有一层致密物貭,无桥粒。

Sixty four albino mice (aged 50 days) and 41 albino rats (aged 124 days) were

用50天小白鼠64只及124天大白鼠41只,各分4组:Ⅰ组均切除左肾;Ⅱ组均在皮下注射ACTH隔日一次,剂量为每百克体重0.33国际单位,但其中大白鼠在注射前10天先切去左肾,小白鼠不切;Ⅲ组均先切除左肾,10天后注射ACTH,其中小白鼠所用剂量与Ⅱ组同,大白鼠则用15倍于Ⅱ组的剂量;Ⅳ组大、小白鼠均作对照。小白鼠自注射25次开始分批取材,最后一批注射达78次。大白鼠在注射24次后即全部杀死。两批材料均取颈膨大处的脊神经节及同节脊髓,用福尔马林固定,制成石蜡切片。在苏丹黑及PAS染色的切片上观察了神经细胞内呈阳性反应的脂褐色素,计算了在不同处理下含色素细胞所占百分率。并根据色素在细胞内含量的不同等级,比较了不同处理下含各级色素的细胞数量。对所得数据进行了统计分析。结果表明各项实验处理都没有使含色素的神经细胞比对照有所增多,也没有提高细胞内的色素含量。以大白鼠为例,上述四组中含色素的神经细胞所占百分率由Ⅰ到Ⅳ分别为3.31、4.00、3.40及3.70%。各实验组与对照的差异均无统计学意义。以上结果与Sulkin在1960年发表的研究结果不同。他认为去肾及去肾后注射ACTH的大白鼠神经细胞比对照有更多的色素沉积...

用50天小白鼠64只及124天大白鼠41只,各分4组:Ⅰ组均切除左肾;Ⅱ组均在皮下注射ACTH隔日一次,剂量为每百克体重0.33国际单位,但其中大白鼠在注射前10天先切去左肾,小白鼠不切;Ⅲ组均先切除左肾,10天后注射ACTH,其中小白鼠所用剂量与Ⅱ组同,大白鼠则用15倍于Ⅱ组的剂量;Ⅳ组大、小白鼠均作对照。小白鼠自注射25次开始分批取材,最后一批注射达78次。大白鼠在注射24次后即全部杀死。两批材料均取颈膨大处的脊神经节及同节脊髓,用福尔马林固定,制成石蜡切片。在苏丹黑及PAS染色的切片上观察了神经细胞内呈阳性反应的脂褐色素,计算了在不同处理下含色素细胞所占百分率。并根据色素在细胞内含量的不同等级,比较了不同处理下含各级色素的细胞数量。对所得数据进行了统计分析。结果表明各项实验处理都没有使含色素的神经细胞比对照有所增多,也没有提高细胞内的色素含量。以大白鼠为例,上述四组中含色素的神经细胞所占百分率由Ⅰ到Ⅳ分别为3.31、4.00、3.40及3.70%。各实验组与对照的差异均无统计学意义。以上结果与Sulkin在1960年发表的研究结果不同。他认为去肾及去肾后注射ACTH的大白鼠神经细胞比对照有更多的色素沉积现象。本文在讨论中对比了双方的工作,就Sulkin室实验中的动物只数、研究方法以及他所提出的“应激”反应进行了评述。最后指出,神经细胞内脂褐素的实验性形成问题还远未解决。因此还没有理由完全否认色素沉积是一种内生的衰老变化的指标。

The dose-response curves of haemopoietic stem cells in irradiated LACA micewere studied with the spleen colony method and the in vitro agar culture technique.The D_0 value of mouse pluripotential stem cells(CFU-S)was 103.4 rads and thatof granuloid committed precursor cells(CFU-C)was 173.7 rads.It is apparentthat the characteristics of CFU-S are different from those of CFU-C.Since a general method for the assay of pluripotential haemopoietic stem cellsin various animals other than the mouse is not...

The dose-response curves of haemopoietic stem cells in irradiated LACA micewere studied with the spleen colony method and the in vitro agar culture technique.The D_0 value of mouse pluripotential stem cells(CFU-S)was 103.4 rads and thatof granuloid committed precursor cells(CFU-C)was 173.7 rads.It is apparentthat the characteristics of CFU-S are different from those of CFU-C.Since a general method for the assay of pluripotential haemopoietic stem cellsin various animals other than the mouse is not available at present,we used the invitro agar curture technique and compared the radiosensitivity of canine and mousebone marrow CFU-C.It was found that Do values calculated from the survivalcurves of radiation of canine and mouse haemopoietic stem cells were 58.6 and190.2 rads respectively.From the results thus obtained it is possible to explainthe different radioresistance between the dog and the mouse on the basis of haemo-poietic stem cells.In view of the fact that AET is capable of protecting haemopoieticstem cells on the one hand and reducing the mortality of irradiated animals onthe other,it is reasonable to assume that the haemopoietic stem cells may play animportant role in the radiation damage and recovery of the animal.

本文研究了在体内外照射条件下小鼠骨髓多向性造血干细胞(CFU-S)和粒系定向千细胞(CFU-C)的辐射敏感性。它们的 D_0值分别为103.4拉德和173.7拉德,反映了CFU-S 和 CFU-C 是两类在性质上不全相同的干细胞群。在目前尚未建立起除啮齿类动物以外的多向性造血干细胞测定技术的情况下,我们应用体外琼脂培养技术,比较了在体外照射条件下狗与小鼠骨髓 CFU-C 的辐射敏感性,它们的 D_0值分别为58.6拉德和190.2拉德,反映了两者在辐射敏感性上存在着明显的差异,为阐明不同种属动物的不同辐射耐受性提供了一个根据。联系到 AET 具有降低造血干细胞辐射敏感性以及提高动物的辐射耐受性的事实,进一步论证了在造血型放射病的发生和发展中,造血干细胞的损伤和修复是起着重要作用的。

 
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