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glycoprotein d gene     
相关语句
  gd基因
     Expression of the Envelope Glycoprotein D Gene of Pseudorabies Virus Ea Strain
     伪狂犬病病毒鄂A株囊膜蛋白gD基因在杆状病毒载体系统中的表达研究
短句来源
     The molecular cloning and sequence analysis of the glycoprotein D gene of bovine herpesvirus 1 Luojing strain
     牛疱疹病毒1型洛精株gD基因的分子克隆及序列分析
短句来源
     Cloning of Glycoprotein D Gene of Bovine Herpesvirus 1 Bartha Nu/67 Strain and Its Expression in E.coli
     牛疱疹病毒Ⅰ型Bartha Nu/67株gD基因的克隆及在大肠杆菌中的表达
短句来源
     The envelope glycoprotein D gene of pseudorabies virus Ea strain was cloned by PCR technique. Sequence analysis displayed 98% nucleotide sequence homology and 97% deduced amino acid sequence homology between the cloned gD gene and PRV Rice strain gD gene.
     克隆了伪狂犬病病毒鄂A株囊膜蛋白gD基因并进行了序列分析 ,与国际标准毒株Rice株相比 ,两者之间核苷酸序列同源性为 98% ,推导氨基酸序列同源性为 97% .
短句来源
     A pair of primers was designed and synthesized according to glycoprotein D gene sequence of pseudorabies virus Fa strain. The gD gene encoding area of pseudorabies virus Fa strain exclude signal peptide was amplified by PCR from the recombinant plasmid pB13, which harbors the complete gD gene. The PCR product was cloned into an expression vector pThiohisA for E.
     应用PCR方法从包含伪狂犬病病毒(PRV)Fa株糖蛋白gD基因的重组质粒pB13中扩增出糖蛋白gD基因去信号肽片段,将其克隆到大肠埃希氏菌表达载体pThiohisA中。
短句来源
  糖蛋白d基因
     Construction and its immunization effect of the eukaryotic expression vector bearing HSV-1 glycoprotein D gene
     HSV-1 SM44株糖蛋白D基因真核表达载体的构建及其免疫效果观察
短句来源
     CLONING AND EXPRrSSION OF CHINESE HERPES SIMPLEXVIRUS TYPE I STRAIN 168 GLYCOPROTEIN D GENE USINGVACCINIA VIRUS TIAN TAN STRAIN AS A VECTOR
     我国单纯疱疹病毒Ⅰ型168株糖蛋白D基因的克隆及其在痘苗病毒天坛株中的表达
短句来源
     STUDY ON INTRODUCTION OF MAREK’S DISEASE VIRUS GLYCOPROTEIN D GENE TO GENOMIC DNA OF INFECTED CELLS
     马立克病毒糖蛋白D基因转入感染细胞DNA的研究
短句来源
     EXPRESSION OF MAREK'S DISEASE VIRUS GLYCOPROTEIN D GENE TRANSFERRED INTO CHICKEN EMBRYO FIBROBLAST GENOME BY USING RETROVIRAL VECTOR
     由反转录病毒载体转移的马立克病毒糖蛋白D基因在感染细胞中的表达
短句来源
     MOLECULAR CLONING AND SEQUENCE ANALYSIS OF GLYCOPROTEIN D GENE OF HERPES SIMPLEX VIRUS TYPE 1 STRAIN 168 ISOLATED IN CHINA
     我国单纯疱疹病毒Ⅰ型168株糖蛋白D基因的克隆及序列分析
短句来源
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  “glycoprotein d gene”译为未确定词的双语例句
     Clinical Feature and Glycoprotein D Gene Sequence Analysis of a Wild Strain of HSV-2 Isolated from One Relapsed Patient with Genital Herpes
     一例复发性生殖器疱疹的临床及其致病的HSV2野生株的糖蛋白D基因序列分析
短句来源
     he glycoprotein D gene of herpes simplex virus type 2(HSV -2gD )was modified by means of polymerase chain reaction (PCR)to delete about 500bp of its 5'-noncoding region .
     利用PCR方法对单纯疱疹病毒Ⅱ型糖蛋白D(HSV-2gD)基因进行了修饰,在其5'端删去约500bp的非编码区,仅保留ATG上游7个bp。
短句来源
     Objective:To obtain the high expression of Herpes Simplex Virus Type 1(HSV-1)Glycoprotein D gene.
     目的:获得高表达的I型单纯疱疹病毒(HSV)被膜糖蛋白gD(简称gD1)基因的工程菌。
短句来源
     Amplification, Cloning, Sequencing and Expression of Marek′s Disease Virus Glycoprotein D gene
     马立克氏病病毒(MDV)糖蛋白D基因的分离克隆、定序及其在大肠杆菌中的表达
短句来源
     Method The partial glycoprotein D gene sequence of HSV 2 strain Sav and wild strain isolated from recurred genital herpes simplex were amplified and cloned with PCR.
     方法利用聚合酶链反应从HSV-2Sav国际标准毒株及我国1例生殖器疱疹复发型野生株中扩增、克隆出糖蛋白D基因序列。
短句来源
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  glycoprotein d gene
After inserting a copy of the glycoprotein D gene into the thymidine kinase locus of BHV1 it was possible to delete the entire HindIII K-fragment and the contiguous 0.36?kb O-fragment in one step.
      
Our analysis indicates that all the genes present in the K-fragment, except the glycoprotein D gene, are nonessential for replication of BHV1 in tissue culture.
      
Identification of a potential Marek's disease virus serotype 2 glycoprotein D gene with homology to herpes simplex virus glycopr
      
A homologue of the herpes simplex virus (HSV) glycoprotein D gene has been identified in the genome of equine herpesvirus-1 (EHV-1, equine abortion virus).
      


According to the DNA sequence of glycoprotein D gene of HSV-1 strain Patton,two primers were designed and synthesized, a complete gly-coprotein D gene of HSV-1 strain 168 isolated in China was amplified thr-ough PCR reaction. The complete glycoprotein D gene sequence has 1185 nucleotides and encodes a polypeptide of 395 amino acids. Result obtained from the homology comparison among 168,HFEM,SC16,17 and Patton showed that the most consetved region was located in the ectodomain, whereas...

According to the DNA sequence of glycoprotein D gene of HSV-1 strain Patton,two primers were designed and synthesized, a complete gly-coprotein D gene of HSV-1 strain 168 isolated in China was amplified thr-ough PCR reaction. The complete glycoprotein D gene sequence has 1185 nucleotides and encodes a polypeptide of 395 amino acids. Result obtained from the homology comparison among 168,HFEM,SC16,17 and Patton showed that the most consetved region was located in the ectodomain, whereas the most variable re靑ons in the signal peptide region,as well as transmembrane and cytoplasmic domain re靑ons. The present paper pro-vides the basis for further understanding of structure and function of HSV-1 gD gene and for a HSV vaccine development.

依据HSV-1 Patton株糖蛋白D全基因序列,设计、合成一对引物,利用多聚酶链反应(PCR),从我国HSV-1 168株基因组DNA中扩增、克隆出糖蛋白D全基因序列,共1185bp,编码395个氨基酸,并将其推测的氨基酸序列同国外HSV-1 HFEM、SCl6、17和Patton株的糖蛋白D的氨基酸序列进行了详细比较,发现最保守的区域主要是除信号肽以外的胞外编码区。氨基酸的变异主要分布在信号肽区、跨膜区及胞浆内编码区。本工作对研究HSV-1糖蛋白D的结构和功能以及研制我国HSV-1基因工程疫苗都具重要意义。

he glycoprotein D gene of herpes simplex virus type 2(HSV -2gD )was modified by means of polymerase chain reaction (PCR)to delete about 500bp of its 5'-noncoding region .PCR -modified HSV-2 gD gene was inserted into the plasmid pJSA1175 under the control of P7.5 promoter of vaccinia virus flanked by TK gene sequences of the Tian Tan strain of vaccinia virus. The initiation codon ATG of HSV -2gD was immediate downstream from p7.5 promoter. The recombinant plasmid containing HSV-2gD gene...

he glycoprotein D gene of herpes simplex virus type 2(HSV -2gD )was modified by means of polymerase chain reaction (PCR)to delete about 500bp of its 5'-noncoding region .PCR -modified HSV-2 gD gene was inserted into the plasmid pJSA1175 under the control of P7.5 promoter of vaccinia virus flanked by TK gene sequences of the Tian Tan strain of vaccinia virus. The initiation codon ATG of HSV -2gD was immediate downstream from p7.5 promoter. The recombinant plasmid containing HSV-2gD gene was used, to transfect 143TK ̄- cell infected by the wild type TK ̄+ vaccinia virus (Tian Tan strain )Afterseveral cycles of selection and screening using the marker lac Z gene together with the selective pressure of BudR for TK ̄- phenotype ,recombinant vaccinia virus harboring HSV-2gD gene was picked out and sequentially plaque-purified.Southern blot confirmed that the HSV-2gene had been inserted correctly into the TK region of vaccinia virus genome. Therecombinant virus was shown to be able to express HSV-2 gD gene effectively. Indirect immunofluoresent assay showed the expressed HSV-2gD protein distributed predominantlyon cell membrane. Immunization of rabbits with recombinant virus,a neutralizing antibodyresponseagainst HSV-2 was obtained.Seventeen of 18 mice (17/18,94%)vaccinated with the recmbinant virus were protected from the lethal challenge with HSV-2.

利用PCR方法对单纯疱疹病毒Ⅱ型糖蛋白D(HSV-2gD)基因进行了修饰,在其5'端删去约500bp的非编码区,仅保留ATG上游7个bp。将修饰后的HSV-2gD基因插入到带有痘苗病毒天坛株TK基因区段的痘苗表达质粒pJSA1175,置于痘苗病毒P7.5k早/晚期启动子控制下。将此重组质粒用脂质体Lipofectin方法转染已受野型TK ̄+痘苗病毒天坛株感染的TK ̄-143细胞,通过同源重组机制和标志基因LacZ产物的蓝斑显色作用,以及BudR试剂对TK表型的选择压力,筛选出整合有HSV-2gD基因的重组痘苗病毒。Southem杂交表明,HSV-2gD基因已正确地插入痘苗病毒TK基因区内;间接免疫荧光检测显示,HSV-2gD蛋白已得到有效表达,且主要分布于细胞膜。重组病毒免疫家兔可产生明显的抗HSV-2gD中和抗体。用重组病毒免疫小鼠,3周后可使94%(17/18)的小鼠对抗HSV-2的致死量攻击,表明重组病毒具有明显的免疫保护作用。

Objective To study the diffrence of structure and function of HSV 2 gD gene among the wild strain, G and Sav strain. So as to provide theoretical basis for future production of HSV 2 vaccine. Method The partial glycoprotein D gene sequence of HSV 2 strain Sav and wild strain isolated from recurred genital herpes simplex were amplified and cloned with PCR. Results The homology comparision showed that the homology of DNA and amino acid were 99.2% and 99.1% respectively. Conclusions There are...

Objective To study the diffrence of structure and function of HSV 2 gD gene among the wild strain, G and Sav strain. So as to provide theoretical basis for future production of HSV 2 vaccine. Method The partial glycoprotein D gene sequence of HSV 2 strain Sav and wild strain isolated from recurred genital herpes simplex were amplified and cloned with PCR. Results The homology comparision showed that the homology of DNA and amino acid were 99.2% and 99.1% respectively. Conclusions There are high homology of HSV 2 gD gene among the wild strain G and Sav strains,but some variations existed.

探讨我国单纯疱疹病毒Ⅱ型(HSV-2)糖蛋白D(gD)基因结构和功能及与国外的病毒株之间的差异,为研制我国生殖器疱疹疫苗提供理论依据。方法利用聚合酶链反应从HSV-2Sav国际标准毒株及我国1例生殖器疱疹复发型野生株中扩增、克隆出糖蛋白D基因序列。结果将野生株和Sav株DNA序列及氨基酸序列同HSV-2G株比较,发现其DNA同源性为99.2%,氨基酸同源性为99.1%。结论我国HSV-2野生株同国外的Sav株及G株gD基因存在高度的同源性,同时也存在一定变异。

 
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