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regulation region
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    Transient expression assays in uninfected Sf 21 cells indicated that the helicase gene promoter could be classified as a delayed early gene promoter. Deletion analysis by PCR showed that the regulation region of its basic transcription was mainly within -510 to -410 bp upstream of ATG.
    通过PCR技术在该启动子区产生的一系列缺失分析表明 ,解旋酶基因启动子的基础转录调控区主要位于ATG上游 - 5 1 0~ - 4 1 0bp之间。
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  regulation region
A site-directed mutant DNA fragment was synthesized and transfected into clinical Neisseria Gonorrhoeae (NG) stains to construct the transformants that contained the corresponding mutagenesis of regulation region of mtrR gene.
      
In vitro recombination and identification of mutated fragment corresponding to regulation region of mtrR gene of Neisseria Gonor
      
The mutation (C→T) in the 5' regulation region of chicken MC4R gene results in one more NF-E2 and cap transcription factor binding sites in the mutation allele than in the wild allele.
      
Unusual structure in the regulation region of the Bacillus subtilis riboflavin biosynthesis operon.
      
The genes of most of the structural muscle proteins have a MyoD-binding regulation region.
      
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DNA helicases are essential for replication of baculoviruses. It was found that the helicase gene promoter of Bombyx mori nuclear polyhedrosis virus, including 510 bp upstream of ATG, had both early and late RNA initiation sites and could be recognized by cellular RNA polymerase. Transient expression assays in uninfected Sf 21 cells indicated that the helicase gene promoter could be classified as a delayed early gene promoter. Deletion analysis by PCR showed that the regulation region of its basic...

DNA helicases are essential for replication of baculoviruses. It was found that the helicase gene promoter of Bombyx mori nuclear polyhedrosis virus, including 510 bp upstream of ATG, had both early and late RNA initiation sites and could be recognized by cellular RNA polymerase. Transient expression assays in uninfected Sf 21 cells indicated that the helicase gene promoter could be classified as a delayed early gene promoter. Deletion analysis by PCR showed that the regulation region of its basic transcription was mainly within -510 to -410 bp upstream of ATG. However, the basic activity was still detected with a deletion to -98 bp relative to ATG. In the presence of viral factors, deletion between -510 to -410 bp relative to ATG did not significantly reduce the promoter activity compared to the full length promoter (510 bp). The remarkable reduction in the promoter activity was observed with continuous deletions. It suggests, therefore, that cis acting elements responsive to viral factors are mainly located within the range of -410 to -309 bp upstream of ATG.

杆状病毒DNA解旋酶是病毒复制所必需的。瞬时表达分析显示 ,家蚕核多角体病毒解旋酶基因启动子属于延迟早期基因启动子。通过PCR技术在该启动子区产生的一系列缺失分析表明 ,解旋酶基因启动子的基础转录调控区主要位于ATG上游 - 5 1 0~ - 4 1 0bp之间。当只保留ATG上游 98bp区段时 ,仍可测到该启动子的基础活性。在病毒因子存在下 ,将启动子区域删除到ATG上游 - 4 1 0bp时 ,对启动子活性影响不大 ;若继续删除 ,则其活性显著下降。据此推测对病毒因子响应的启动子区段应主要位于ATG上游 - 4 1 0~ - 30 9bp之间

 
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