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regulation region
相关语句
  调控区
    Construction of N-LMP1 Transgenic Mice with the Specific Regulation Region in Nasopharynx
    用鼻咽相对特异性调控区建立N-LMP1转基因小鼠
短句来源
    The Influence of SNP in Regulation Region of α_(2A)-Adrenergic Receptor Gene on Transcription Activity
    α_(2A)-肾上腺素能受体基因调控区单核苷酸多态性对基因转录活性的影响
短句来源
    In vitro recombination and identification of mutated fragment corresponding to regulation region of mtrR gene of Neisseria gonorrhoeae
    淋球菌mtrR调控区突变片段的体外重组和鉴定
短句来源
    Cloning and Functional Characterization of The Regulation Region of Human BRD7 Gene
    BRD7基因调控区的克隆与功能研究
短句来源
    GFAP gene regulation region was unknown, then we must separate its 5' terminal regulation sequence.
    由于当时GFAP基因的调控序列尚属未知,因此需要克隆小鼠GFAP基因的5’端调控区
短句来源
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  “regulation region”译为未确定词的双语例句
    The Study on the 5 Flanking Regulation Region of the Human Vigilin Gene
    人vigilin基因5'端调控区域的研究
短句来源
    Identification of 5-flank upstream regulation region of CD226
    CD226基因5′上游调控序列研究
短句来源
    Methods:Following the report gene containing different regulation region of TNF-α gene transfected into HL-60 cell,its expression level stimulated with/without LPS or blocked with κB 3 antisense was detected respectively;
    方法 :用含不同调节区域的重组报告基因进行HL 6 0细胞的基因转染 ,检测LPS刺激前后及κB3 反义寡核苷酸封闭后的报告基因表达水平 ;
短句来源
    Objective:To identify the function of the 5′-flank upstream regulation region of human CD226 gene.
    目的:研究CD226基因5′上游调控序列对CD226基因表达调控的影响。
短句来源
    Methods:The upstream regulation region of CD226 was cloned by PCR and ligated into pGL3 vector.
    方法:通过基因克隆的方法将CD226基因5′上游调控序列,克隆到荧光素酶报告基因载体中(pGL3basic),用脂质体转染Jurkat细胞,48小时后检测荧光素酶活性。
短句来源
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  regulation region
A site-directed mutant DNA fragment was synthesized and transfected into clinical Neisseria Gonorrhoeae (NG) stains to construct the transformants that contained the corresponding mutagenesis of regulation region of mtrR gene.
      
In vitro recombination and identification of mutated fragment corresponding to regulation region of mtrR gene of Neisseria Gonor
      
The mutation (C→T) in the 5' regulation region of chicken MC4R gene results in one more NF-E2 and cap transcription factor binding sites in the mutation allele than in the wild allele.
      
Unusual structure in the regulation region of the Bacillus subtilis riboflavin biosynthesis operon.
      
The genes of most of the structural muscle proteins have a MyoD-binding regulation region.
      
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The recombinanl human B hepatitis core antigen gene was cloned into-35 position of autographa califomica nuclear polyhydrosis virus transfer vector pAC360, then using site specific mutagenasis, 32 nuclcotidcs were deleted A expression vector was obtained which had a entitle regulation region of pohhydrin gene and the 1 position of regulation region lined directly ATG of human B hepatitis core antigen . This vector had a idcntial gene structure to polyhydrin gene.

作者利用寡核苷酸定位诱导突变技术,将重组的人乙型肝炎核心抗原(HBcAg),插入丫蚊夜蛾核多角体蛋白病毒,表达载体pAc360的-35位BamH 酶切位点,经突变缺失多角体蛋白基因调控区和HBcAg起始码ATG间32个非编码核苷酸序列,获得一个与自然核多角体蛋白基因结构形式相同的表达载体,即核多角体蛋白基因调控区的-1位紧连核心抗原的起始码ATG.

The gene,coding for a new protein called vigilin,was isolated from chicken chondrocytes.Vigilin mRNA exists in two size species(4.4 kb and 6.5 kb).The vigilin gene is expressed in human tissue too.For further exploring the transcription starting site and regulatory element of human genomic vigilin gene.5 flanking EcoR Ⅰ fragment(Vig CG5 7E)was subcloned from a genomic DNA and selected 6 corresponding enzymes to establish the restriction map.Meanwhile,the...

The gene,coding for a new protein called vigilin,was isolated from chicken chondrocytes.Vigilin mRNA exists in two size species(4.4 kb and 6.5 kb).The vigilin gene is expressed in human tissue too.For further exploring the transcription starting site and regulatory element of human genomic vigilin gene.5 flanking EcoR Ⅰ fragment(Vig CG5 7E)was subcloned from a genomic DNA and selected 6 corresponding enzymes to establish the restriction map.Meanwhile,the locality of transcription starting site and sequence analysis of regulation region of the human vigilin gene was described.After the clone Vig CG5 7E being completely digested with the restriction enzyme Apa Ⅰ,they were electrophoretically separated and transfered onto a nitrocellulose filter,and were hybridizated with a 20 bp probe from the cDNA .The length of hybridization area was 0.1kb.It located at of the out Apa Ⅰ end of Vig CG5 7E3AE.It is shown that clone Vig CG5 7E3AE include regulation elements of the vigilin gene by sequencing analysis.Two TATA box,one CAAT box and one GC box and two consensus sequences of Ap 1 and Ap 2 was found in the area.It was predicted that GC box is a promoter element of vigilin gene.

在克隆了人基因组全长vigilin基因的基础上,对其5端转录调控区域再克隆,获得Vig-CG5-7E克隆,再用限制酶ApaⅠ和EcoRⅠ切除3端约4kb部分,得到Vig-CG5-7E3AE克隆,并对该克隆进行双向测序分析.结果表明:克隆Vig-CG5-7E用ApaⅠ酶消化后,用cDNA上游开始的20个碱基组成的寡聚核苷酸做探针进行分子杂交,可见一条小于0.1kb杂交带,根据物理图谱分析,位于Vig-CG5-7E3AE克隆的ApaⅠ端,从而推断该克隆含有转录调控元件,5端序列分析得到二个TATA盒,一个CAAT盒和一个GC盒以及二个可能的Ap-1和Ap-2结合位点.认为GC盒可能为人vigilin基因启动子的组成部分

Objective The expression of invasion plasmid antigen genes (ipaBCD) of Shigella flexneri is positively regulated by genes of virF and invE at the same invasion plasmid. The study here is to defini a factor on the chromosome of Shigella spp, which could be effective to promote ipa expression in cooperation with invE. Methods A 4.3kb DNA fragment (pQ445) cloned from S. flexneri mutant (HW 1002) into the low copy expression vector pACYC184 was selected by using β galactosidase assay and...

Objective The expression of invasion plasmid antigen genes (ipaBCD) of Shigella flexneri is positively regulated by genes of virF and invE at the same invasion plasmid. The study here is to defini a factor on the chromosome of Shigella spp, which could be effective to promote ipa expression in cooperation with invE. Methods A 4.3kb DNA fragment (pQ445) cloned from S. flexneri mutant (HW 1002) into the low copy expression vector pACYC184 was selected by using β galactosidase assay and Western blot with convalescent serum. It showed the enhancement of ipaB expression.The regulation region located at 87.3 min was confirmed by restriction mapping and DNA hybridization with a probe of Pst1 DNA fragment of pQ445. Results An ORF (orfB, criA) within this region was identified as a positive regulator gene and its complete nucleotide sequence was determined. Furthermore, the probe did react with genomic DNA from other Shigella spp. and E.coli strain. Conclusions cirA protein predicted (476AA,52.6kDa) from criA gene (1438bp) contains the leucine zipper motifs of amino acid residues of 268 AA 298 AA and 320AA 341AA which appear to have regulatory activity for InvE protein.

目的为证实调控ipaBCD表达除侵袭质粒上的两个正性调控基因virF和invE外,染色体上也可能存在与invE共同参与促进ipa基因表达的因子。方法应用低拷贝克隆载体pA-CYC184,在大肠杆菌构建福氏志贺氏菌染色体基因文库。经半乳糖苷酶活性测定、分子杂交等筛选,获得一株能增进上皮细胞侵袭性基因ipaB表达的DNA分子克隆pQ445。结果该片段约为4.3kb长,DNA序列分析表明有两个完整的开放阅读框架(ORF),经插入缺失突变发现,其中一个ORF的表达产物能增加ipaB的活性,为ipa正性调节因子,定名为criA。criA基因位于细菌染色体87.3分处,志贺菌属大多菌群都携带该基因。criA约1438bp,编码476个氨基酸残基的酸性蛋白,分子量约52600。结论CriA蛋白可能凭借其268~298位和320~341位两处的氨基酸残基位点亮氨酸拉链区结构,在蛋白相互作用中调节侵袭性基因的表达

 
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