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regulation region
相关语句
  调控区
    Conclusion: The base sequence 101 bp (from -595 to -494) in up stream 5' flank near regulation region of colorectal cancer related gene ST13 is an enhancer regulating gene transcript.
    结论 :位于 ST13基因上游 5 '近端调控区的碱基序列 10 1bp片段 (- 5 95~ - 494 ) ,是一个基因转录调控增强子
短句来源
    Objective To study the inhibition effects on hepatoma cells growth by the anti-sense RNA targeting C-MYC binding site on regulation region of hTERT promoter.
    目的研究针对人类端粒酶催化亚单位(hTERT)调控区C-MYC结合位点的反义RNA抑制肝癌细胞生长。
短句来源
    Construction of antisense RNA expression vector of the regulation region of hTERT using the adnovirus system by homorecombination in E.coli
    构建细菌内同源重组型hTERT调控区反义RNA腺病毒载体
短句来源
    Two possible retinoic acid responsive elements (RAREs) were located in the regulation region of mitochondrion genome.
    对线粒体基因组的转录调控区D-loop序列进行了初步分析发现,该转录调控区两处存在着与维甲酸受体反应元件核心序列高度一致的外翻重复序列(everted repeated seopnces),其间隔分别为 12bp和 13bp。
短句来源
    Methods The transcription regulation region of hTERT in HeLa and PG cells were amplified by polymerase chain reaction and confirmed by DNA sequencing, then were reconstructed into luciferase reporter vectors and transiently transfected into HeLa, PG, 293 and normal human diploid 2BS cell.
    方法 用PCR方法克隆HeLa和PG癌细胞hTERT基因转录调控区 ,并进行DNA序列测定 ; 将转录调控区重组于荧光酶报告载体 ,瞬时转染人癌细胞HeLa、PG、2 93和正常人二倍体细胞 2BS后 ,测定荧光酶活性以确定调控区转录活性。
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  “regulation region”译为未确定词的双语例句
    Objective:To Study the inhibition effect of antisense phosphorothioate oligodeoxynu-cleotides com ple mentary to the regulation region of hTERT(human telomerase reverse transcriptase)on the growth of hep atoma cells.
    目的:研究人类端粒逆转录酶(hTERT)基因关键位点的反义寡核苷酸对人肝癌的抑制效应。
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  regulation region
A site-directed mutant DNA fragment was synthesized and transfected into clinical Neisseria Gonorrhoeae (NG) stains to construct the transformants that contained the corresponding mutagenesis of regulation region of mtrR gene.
      
In vitro recombination and identification of mutated fragment corresponding to regulation region of mtrR gene of Neisseria Gonor
      
The mutation (C→T) in the 5' regulation region of chicken MC4R gene results in one more NF-E2 and cap transcription factor binding sites in the mutation allele than in the wild allele.
      
Unusual structure in the regulation region of the Bacillus subtilis riboflavin biosynthesis operon.
      
The genes of most of the structural muscle proteins have a MyoD-binding regulation region.
      
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Objective: To explore the mechanism of prevention and therapy by retinoic acid in cancer and isolate ATRA-target gene and explore the mechanism of expression regulation of mitochondrial gene. Methods: AP-PCR, hybridization, sequencing etc., were used. Results: ATRA up-regulated the expression of mitochondrion gene ATPase subunit 6, the change lasted in whole period of ATRA treatment (from first day to seventh day). The up-regulation was confirmed by Northen blot analysis. Two possible retinoic acid responsive...

Objective: To explore the mechanism of prevention and therapy by retinoic acid in cancer and isolate ATRA-target gene and explore the mechanism of expression regulation of mitochondrial gene. Methods: AP-PCR, hybridization, sequencing etc., were used. Results: ATRA up-regulated the expression of mitochondrion gene ATPase subunit 6, the change lasted in whole period of ATRA treatment (from first day to seventh day). The up-regulation was confirmed by Northen blot analysis. Two possible retinoic acid responsive elements (RAREs) were located in the regulation region of mitochondrion genome. Conclusion: The mitochondrion gene expression by ATRA might be regulated through the interaction of retinoic acid receptor (RAR) with RARE in mitochondrion genome

目的:探讨维甲酸在预防和治疗肿瘤中的作用机制,分离受维甲酸调控的靶基因。方法:采用AP-PCR、测序、North-em杂交及序列同源分析等方法。结果:首次发现ATRM可上调线粒体基因ATP酶第六亚单位表达,这种表达上调开始于A-TRA诱导早期并持续整个诱导过程(7d)。 Northem杂交结果证实 ATRA对线粒体基因 ATP酶第六亚单位的上调作用。对线粒体基因组的转录调控区D-loop序列进行了初步分析发现,该转录调控区两处存在着与维甲酸受体反应元件核心序列高度一致的外翻重复序列(everted repeated seopnces),其间隔分别为 12bp和 13bp。结论:维甲酸受体与线粒体基因组维甲酸反应元件相互作用可能是其调控线粒体基因的表达。维甲酸除调控核基因外,亦可对线粒体基因进行直接调控。

Objective To probe the activation mechanism of hTERT gene in cancer formation based on cloning its upstream regulating region and observing the specific transcription activity in verious transiently transfected human cancer cells and human diploid cells. Methods The transcription regulation region of hTERT in HeLa and PG cells were amplified by polymerase chain reaction and confirmed by DNA sequencing, then were reconstructed into luciferase reporter vectors and transiently transfected into HeLa, PG,...

Objective To probe the activation mechanism of hTERT gene in cancer formation based on cloning its upstream regulating region and observing the specific transcription activity in verious transiently transfected human cancer cells and human diploid cells. Methods The transcription regulation region of hTERT in HeLa and PG cells were amplified by polymerase chain reaction and confirmed by DNA sequencing, then were reconstructed into luciferase reporter vectors and transiently transfected into HeLa, PG, 293 and normal human diploid 2BS cell. The transcription activities were determined by measuring the luciferase activities. Results The 636bp (-531~+90) and 950bp(-531~+404) hTERT transcription regulation regions in HeLa and PG cells have the same sequences with the hTERT promoter registered in Genbank. These transcription regulation regions were cloned into luciferase reporter plasmid to generate the recombinants pGL2 636 and pGL2 950. Meanwhile, pGL2 X (-531~-272) and pGL2 S (the first intron region) were reconstructed. After transient transfection, results showed that luciferase activity increased dramatically in cancer cells transfected with pGL2 636 and pGL2 950, whereas no luciferase activity was identified in human diploid 2BS cells. The transcription activities of pGL2 S and pGL2 X decreased significantly in telomerase positive tumor cells, and that of pGL2 S was slightly higher than pGL2 X. Conclusion In human HeLa and PG cells, the transcription regulation regions of hTERT gene have no gene mutation and are highly conservative. The transcriptional activation of hTERT correlated with the expression of telomerase and cancer transformation. The -272bp upstream region of hTERT gene was the core regulatory region, and the first intron had only weak transcriptional activity. The transcription regulation region of hTERT had varying activities in different cancer cells.

目的 通过克隆人癌细胞中端粒酶逆转录酶 (hTERT)基因上游转录调控区并观察其在不同人癌细胞和正常细胞中的转录活性 ,探讨人hTERT基因在细胞癌变中的激活机制。方法 用PCR方法克隆HeLa和PG癌细胞hTERT基因转录调控区 ,并进行DNA序列测定 ;将转录调控区重组于荧光酶报告载体 ,瞬时转染人癌细胞HeLa、PG、2 93和正常人二倍体细胞 2BS后 ,测定荧光酶活性以确定调控区转录活性。结果 于人HeLa和PG癌细胞分别克隆hTERT基因转录起始点附近 6 36bp(- 5 31~ +90 )和 95 0bp(- 5 31~ +40 4 )的转录调节区 ,其序列与GenBank人hTERT启动子序列相同。将两转录调控区分别重组于荧光酶报告载体pGL2 ,得到pGL2 6 36和pGL2 95 0 ,并同时构建了pGL2 X(- 5 31~ - 2 72 )和pGL2 S (第一内含子区 )。瞬时转染细胞后发现 :pGL2 6 36和pGL2 95 0在癌细胞中的转录活性明显增高 ,而在人二倍体 2BS细胞中几无转录活性。重组体pGL2 S和pGL2 X在端粒酶阳性肿瘤细胞中的转录活...

目的 通过克隆人癌细胞中端粒酶逆转录酶 (hTERT)基因上游转录调控区并观察其在不同人癌细胞和正常细胞中的转录活性 ,探讨人hTERT基因在细胞癌变中的激活机制。方法 用PCR方法克隆HeLa和PG癌细胞hTERT基因转录调控区 ,并进行DNA序列测定 ;将转录调控区重组于荧光酶报告载体 ,瞬时转染人癌细胞HeLa、PG、2 93和正常人二倍体细胞 2BS后 ,测定荧光酶活性以确定调控区转录活性。结果 于人HeLa和PG癌细胞分别克隆hTERT基因转录起始点附近 6 36bp(- 5 31~ +90 )和 95 0bp(- 5 31~ +40 4 )的转录调节区 ,其序列与GenBank人hTERT启动子序列相同。将两转录调控区分别重组于荧光酶报告载体pGL2 ,得到pGL2 6 36和pGL2 95 0 ,并同时构建了pGL2 X(- 5 31~ - 2 72 )和pGL2 S (第一内含子区 )。瞬时转染细胞后发现 :pGL2 6 36和pGL2 95 0在癌细胞中的转录活性明显增高 ,而在人二倍体 2BS细胞中几无转录活性。重组体pGL2 S和pGL2 X在端粒酶阳性肿瘤细胞中的转录活性明显降低 ,pGL2 S的转录活性略高于pGL2 X。结论 人HeLa和PG癌细胞中hTERT转录调控区无基因突变并高度保存 ;hTERT调控区具有癌细胞特异性转录调节活性 ,表明hTERT转录水平的激活与端粒酶阳性及细胞癌变密切相关 ;hTERT上游 2 72为其核心

Objective:To construct antisense RNA expression vector targeted to c-myc bounding site of hTERT gene using adnovirus system by homorecombination in E.coli.Methods:After obtained 370bp DNA fragment of c-myc bounding site on the promoter regulation region of hTERT gene through PCR method,this DNA was ligated to the shuttle vector-pShuttle-CMV of adnovirus system.Positive clones were selected on LB medium with 25ug/ml kanamycin.The antisense RNA expression vector-pShuttle-ashmyc was obtained by PCR method.The...

Objective:To construct antisense RNA expression vector targeted to c-myc bounding site of hTERT gene using adnovirus system by homorecombination in E.coli.Methods:After obtained 370bp DNA fragment of c-myc bounding site on the promoter regulation region of hTERT gene through PCR method,this DNA was ligated to the shuttle vector-pShuttle-CMV of adnovirus system.Positive clones were selected on LB medium with 25ug/ml kanamycin.The antisense RNA expression vector-pShuttle-ashmyc was obtained by PCR method.The plasmid(pShuttle-ashmyc)digested with Pmel and pAdEasy-1 plasmids were cotransformed into BJ5183 bacteria.Postive clones were selected after digested with BamHI or PacI.After digested with PacI,plasmids were transfected into packing cells-293 cell line.Then detected the titer of recombinant adnovirus-pAd-ashmyc.Results:The titer of pAd-ashmyc was 1.2×10 9pfu/ml.Conclusion:The construction of antisense RNA expression vector targeted to c-myc bounding site of the promoter regulation region of hTERT gene afforded the foundation for gene therapy of tumors

目的 :构建细菌内同源重组型端粒酶催化亚单位调控区c myc结合位点的反义RNA腺病毒重组体。方法 :PCR扩增hTERT启动子调控区c myc结合位点 370bp的DNA片段 ,与腺病毒穿梭载体pShuttle CMV连接 ,以抗生素平板筛选连接阳性的克隆 ,PCR鉴定方向 ,构建反向插入的hTERT c myc反义RNA腺病毒穿梭载体pShuttle ashmyc ,将PmeⅠ线性化的 pShuttle ashmyc与包装质粒 pAdEasy 1共转染受体菌BJ5 183进行同源重组。用含有卡那霉素的LB平板筛选同源重组阳性的克隆 ,酶切鉴定后 ,以PacⅠ酶切线性化 ,转染包装细胞 2 93,以软琼脂平板上的空斑数量计算重组腺病毒的滴度。结果 :成功构建了端粒酶催化亚单位调控区c myc结合位点的反义RNA重组腺病毒 pAd ashmyc,滴度为1 2× 10 9efu/ml。结论 :hTERT调控区c myc结合位点的反义腺病毒载体的成功构建 ,为肿瘤的基因治疗奠定了良好的基础

 
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