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verticillium
相关语句
  黄萎病
     Verticillium dahliae kleb.
     棉花黄萎病菌(Verticillium dahliae. Kleb.)
短句来源
     Study on Pathogenicity Differentiation for Verticillium dahliae
     棉花黄萎病(Verticillium dahliae Kleb.)安阳菌系致病力分化研究
短句来源
     Resistance to Verticillium wilt of 8 parents and their F1s、 F2s in upland cotton were analyzed by the genetic models of ADM, ADAA and AD and MINQUE(1) approaches.
     本研究采用 MINQUE( 1)的统计方法 ,用 ADM模型、ADAA模型、AD模型 [1] ,估算陆地棉8个杂交亲本和 F1、F2 代各 2 8个组合的黄萎病抗性遗传效应。
短句来源
     Eight materials (13.56%) were resistant to Verticillium wilt, 43 (72.88%) were tolerant, 8 (13.56%) were susceptible.
     43份材料耐黄萎病,占72.88%; 8份材料感黄萎病,占13.56%。
短句来源
     The Population Genetic of Verticillium dahliae Kleb
     棉花黄萎病菌(Verticillium dahliae Kleb)的群体遗传
短句来源
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  轮枝孢属
     , Verticillium spp.
     、轮枝孢属(Verticillium spp.)
短句来源
     A Comparative Study on the Pathogen of Verticillium Wilt of Alfalfa and Other Verticillium Species
     苜蓿黄萎病菌与轮枝孢属近似种的比较研究
短句来源
     The result of study indicated: the isolate that was obtained in the test was pathogen causing tomato Verticillium wilt, and it was Verticillium dahiea kleb .
     试验结果表明,本试验分离到的病原菌为番茄黄萎病病原菌,鉴定为轮枝孢属大丽轮枝菌(Vercitillium dahiea Kleb.) 。
短句来源
     We also found some dead larvae parasitized by a kind of fungus which we identified to be Verticillium sp..
     在试验过程中还首次发现了自然寄生沙棘木蠹蛾幼虫的真菌,并进行了分离培养,初步确定其中一种分离培养物为轮枝孢属Verticillium的一个种。
短句来源
     The amount of fungi were 1.35~4.56 × 10 6cfu/g of dry soil. They belong to four orders, seven families, and forty five genuses. The dominant genera are Penicillium, Coniothyrium, Cladosporium, Cephalosporium, Fusarium, Verticillium , and Cylindrocarpon .
     分离得到的真菌经分类鉴定到属 ,有 4个目 ,7个科 ,45个属 ,优势属为青霉属 (Penicillium)和盾壳霉属 (Coniothyrium) ,其次为芽枝霉属 (Cladosporium)、头孢霉属 (Cephalosporium)、镰孢霉属 (Fusarium)、轮枝孢属 (Verticillium)、柱孢属 (Cylindrocarpon)。
短句来源
  “verticillium”译为未确定词的双语例句
     (2)Sixrootstocks (Energy, Spirit, He Man, Beaufort, AX105, AL1612 )were resistant to pathogens, Verticillium dahliae . Fusarium oxysporum f.
     (2) 6种砧木ENERGY、SPIRIT、HE MAN、BEAUFORT、AX105、AL1612对Verticillium dahliae、Fusarium oxysporum f.
短句来源
     Optimum conditions for RAPD with the maximum number of score products were determined as 3.5 mmol/L MgCl2, 0.25 mmol/L dNTP, 15 pmol/L primer and 5 ng DNA for Verticillium dahliae.
     大丽轮枝菌(Verticilliumdahliae )RAPD扩增最优反应组分浓度为:MgCl2 3.5mmol/L,dNTP0.25mmol/L,引物15pmol/L,Taq酶1.5U,DNA5ng。
短句来源
     , Verticillium Nees, Fusarium Lk ex Fr.
     镰刀菌属(Fusarium Lk ex Fr.) ;
短句来源
     The clones were named Vdrg (Response Gene induced by Verticillium dahliae).
     将克隆序号命名为Vdrg(Response Gene induced by Verticillium dahliae)。
短句来源
     Of them, Chuan99-1890, 02-9, 02-140, 00-160, Chuan99-1892, 01-243, 2K-Y2 and SG-39 were resistant to both Fusarium and Verticillium wilts.
     其中,抗枯、抗黄的双抗品种有川99-1890、02-9、02-140、00-160、川99-1892、01-243、2K-Y2和SG-39共8个。
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  verticillium
Identification and characterization of differentially expressed ESTs of Gossypium barbadense infected by Verticillium dahliae wi
      
In this study, we adopted the suppression subtractive hybridization (SSH) technique to isolate differentially expressed ESTs from Gossypium barbadense variety 7124 during the Verticillium wilt defense process.
      
Facultative marine fungi of the generaScytalidium, Verticillium, andOidiodendron and obligate marine fungi of the genusDendryphiella are much less abundant.
      
Investigations into the taxonomy of the mushroom pathogen Verticillium fungicola and its relatives based on sequence analysis of
      
The full sequence of the nitrate reductase gene was obtained from a type isolate of Verticillium fungicola var.
      
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The disease of Verticillium wilt commonly occurs in the cotton growing area of Liaoning province in recent years.Through a series of morphological and physiological studies,the fungus is identified as Verticillium albo—atrum R.et B. The fungus has a better growth on the modified Czapeks' agar medium as well as on the potato—sugar agar medium.After 4 to 5 days,the fungus shows an abundant growth of mycelia,conidia and microsclerotia on the mentioned media. The temperature for the growth of the fungus...

The disease of Verticillium wilt commonly occurs in the cotton growing area of Liaoning province in recent years.Through a series of morphological and physiological studies,the fungus is identified as Verticillium albo—atrum R.et B. The fungus has a better growth on the modified Czapeks' agar medium as well as on the potato—sugar agar medium.After 4 to 5 days,the fungus shows an abundant growth of mycelia,conidia and microsclerotia on the mentioned media. The temperature for the growth of the fungus lies between 17°to 28℃,the optimum being 25℃.The optimum temperature for the germination of coinidia is from 15°to 20℃.The conidia of the fungus have a high germination percentage on the one percent dextrose solution.However,they also germinate well on sterilized water;and hence,it seems to indicate that the requirement of nutrients for the germination of conidia is not strict. From the results of wound inoculation with spore suspension,it is indicated thet the conidia are able to infect the roots and stems of the plants in the seedling, flowering and belling stages.However,a higher percentage of infection can be obtained from the flowering to belling stage in 8 to 16 days.

1.棉黄萎病病原菌,经鉴定后,采用 Verticillium albo-atrum Reinke et Berthold学名。2.最适本病原菌生育的培养基,以改良 Czepek's 组合和马铃薯培养基为最好,在这两种培养基上菌丝生长旺盛,经过4—5天后都能生很多分生孢子,并渐生成小菌核。3.本病原菌的发育温度以17°—28℃较好、其中尤以25℃最好。孢子发芽温度以15°—20℃篇最好、在1%葡萄糖液中发芽最好。但在灭菌水中发芽亦好,对营养的要求并不太高。4.本病原菌自苗期(具四真药)开始直至后期,皆能侵入棉株内部,其中尤以用孢子悬浮液于棉根附近接种的办法,得知在开花期至结铃期皆能显现较高的发病率、根据实验记载,在棉花生育期中,接种后一般8—16天左右即能现出病征,此比前人记载期限较早。

When actinomycetes antagonist is cultured in the cotton seed cake and usedas a fertilizer for cotton,a stimulating effect on growth of the plant and a decr-ease of percentage of Verticillium wilt has been observed.The isolates of Actino-mycetes which have shown the best results are G_4 and 5406.Throughout thegrowing period,three applications of the antagonist carried fertilizer show a decr-ease of 31-50% disease and an increase of 14-40% yield.The three applicationsof the antagonist carried fertilizer...

When actinomycetes antagonist is cultured in the cotton seed cake and usedas a fertilizer for cotton,a stimulating effect on growth of the plant and a decr-ease of percentage of Verticillium wilt has been observed.The isolates of Actino-mycetes which have shown the best results are G_4 and 5406.Throughout thegrowing period,three applications of the antagonist carried fertilizer show a decr-ease of 31-50% disease and an increase of 14-40% yield.The three applicationsof the antagonist carried fertilizer seem to be better than that of two applications.However,the yield increase due to the fourth application can hardly cover the costof labour and material. According to the results of a burried-slide test for inspecting the dynamics ofthe antagonist in certain depths of soil,it is found that the actinomycetous isolatesG_4 and 5406 survive a comparativly longer period at a soil depth of 2.5-10 cm.,buttheir amount per unit volume of soil is gradually reduced with time.The anta-gonists are most abundantly distributed at a depth of 5-7.5 cm.It is suggestedthat three applications of the antagonist carried fertilizer may be practical in con-trolling the Verticillium wilt of cotton.

1.1954—1956年在辽陽和北京两地的田間試驗証明:施用“加用抗生菌的肥料”后,不仅能增加棉花的出苗、刺激棉株的生育,并且对黃萎病有显著的防治作用,增产的效果很明显。2.从不同菌种的效能来看,都有防病增产的作用。其中的G_4和5406号抗生菌(均屬放綫菌)的效果最好。进行三次分施时,可减輕發病率31—50%,增加产量14—40%。402号抗生菌种有引起花蕾脫落的恶果;3号及13号虽有显著的防病作用,但增产效能不如G_4。3.不同的抗生菌种混合施用时,沒有表現出增强的作用。4.抗生菌肥料施用的次数,以愈多愈好。在播种前十天作为基肥一次施入者,防病的作用最差,增产作用也不显著。两次分施的,必須在播种时沟施小部份,而将大部份在显蕾期中施入;三次分施的,一般較二次分施的效果高。基肥时不施,单在定苗、显蕾、开花期分施的,对黃萎病的防除效果較大,而对增产作用較小。分四次施肥,所須人工太多,防病、增产的效果并不比三次分施的显著提高。5.根据“埋片法”檢查施入的抗生菌在棉田中消长情况的結果,証明C_4号及5406号放綫菌施入棉田后,在2.5—10厘米深处能維持很长的时間,但其菌量随时間延长而逐漸减少。单施棉籽餅的,在2....

1.1954—1956年在辽陽和北京两地的田間試驗証明:施用“加用抗生菌的肥料”后,不仅能增加棉花的出苗、刺激棉株的生育,并且对黃萎病有显著的防治作用,增产的效果很明显。2.从不同菌种的效能来看,都有防病增产的作用。其中的G_4和5406号抗生菌(均屬放綫菌)的效果最好。进行三次分施时,可减輕發病率31—50%,增加产量14—40%。402号抗生菌种有引起花蕾脫落的恶果;3号及13号虽有显著的防病作用,但增产效能不如G_4。3.不同的抗生菌种混合施用时,沒有表現出增强的作用。4.抗生菌肥料施用的次数,以愈多愈好。在播种前十天作为基肥一次施入者,防病的作用最差,增产作用也不显著。两次分施的,必須在播种时沟施小部份,而将大部份在显蕾期中施入;三次分施的,一般較二次分施的效果高。基肥时不施,单在定苗、显蕾、开花期分施的,对黃萎病的防除效果較大,而对增产作用較小。分四次施肥,所須人工太多,防病、增产的效果并不比三次分施的显著提高。5.根据“埋片法”檢查施入的抗生菌在棉田中消长情况的結果,証明C_4号及5406号放綫菌施入棉田后,在2.5—10厘米深处能維持很长的时間,但其菌量随时間延长而逐漸减少。单施棉籽餅的,在2.5—5厘米深处,也能产生大量的放綫菌,有时較对照(只施化学肥料的)增加2倍,接近于抗生菌肥料中所含的放綫菌数。

Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different...

Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different culture media such as PDA, acidified PDA, cotton meal agar and rice agar. All these plates failed to show verticillium colonies. Among the fungi obtained in these plates, Fusarium, Alternaria and Colletotrichum were the most frequent. Since both Verticillium albo-atrum and other fungi were harbored within the same seed, the difference of growth rates might be considered as an important factor involved in the difficulty of the isolations: Cultures of V. albo-atrum and Fusarium sp. were transferred simutaneously to PDA plates to test their growth rates. The diameter of their colonies were measured after incubation for 12 days under 22±1℃. Those of V. albo-atrum measured 2.8 cm., while the average of the latter was 9,3 cm. Interference of the fast-growers might render a slow-grower such as V. albo-atrum to be masked in the observations and to be depressed in the isolations. A method was developed to avoid the interference. The fundamental procedures of the method are outlined as follows. 1. Plating and observation. (1) Cotton seeds being delinted with commercial surphuric acid in order to avoid the interference from lint-born contaminants. (2) Delinted seeds being washed in a flask covered with wire gauze for 24 hours under running tap water in order to get rid of bacteria. Absorption of sufficient water during washing activates the hibernating mycelium. (3) Seeds being plated on 1.7% water agar instead of nutrient agar in order to diminish the growth of the fast-growers such as Fusaria and Alternaria. The plates being incubated under 22±1℃. for 15 days. (4) The plates being uncovered and observed directly under low-power microscope in order to observe the fungi undisturbed. 2. Isolation and preservation of the Verticillium cultures. (1) Verticilliate conidiophores being located under low-power microscope. (2) The tip of a flamed needle moistened with sterile agar being introduced within the microscopic field in order to fish the spores under focus. (3) Spore suspension being made by washing the needle in a 5 cc sterile water blank. (4) Dilution plates being made by placing 1 cc of the spore suspension in a petri dish and diluting with 10 cc of cotton stem agar. (5) The plates being observed under low-power microscope after incubation for 5—7 days at 22±1℃. (6) Verticillium colonies being locatad and a bit of the margin of a colony being transferred to PDA slant. (7) The slant cultures being flooded with sterile liquid paraffin after incubation for 7—15 days at 22±1℃. The cultures preserved with liquid paraffin were able to keep alived for 2 years. Seeds collected from the infected plants in 1955, 1956 and 1957 were studied. The percentages of seeds carrying Verticillium observed in these years were 39.8, 5.92 and 9.5% respectively. One of the representative single spore colonies obtained was identified as V. albo—atrum. Inoculation and reisolation tests showed positive results. Inspection of cotton seed samples collected from eight co-op farms in Shensi Province in 1958 showing that the percentages of seeds infected by verticillium ranged 3 to 23% with an average of 12.3%.

(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号...

(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号經初步鉴定系Verticillium albo-atrum Reinkeet Berth。(7) 特8号菌种經过接种試驗,确定其具有一定的致病力,并从再分离中获得該菌。(8) 棉籽是否能够传播黄萎病,似系一个检查方法問題。还希各方面进行测定,通过多次的检查和接种試驗始能充分确定。(9) 在分离的菌种中,除特8号菌种具有較深的菌落色泽外,还有一些菌种的顏色較淡,未須鑑定。棉籽所带的轮枝菌类型不一,还有进一步研究的必要。此外,棉籽內的輪枝菌和其他一些菌类究竟如何进入籽內,也有研究的价值。

 
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