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two promoters
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  双启动子
     Construction of reporter gene vector containing two promoters and its expressing activity assay in eukaryotic cells
     双启动子报告基因表达载体的构建及其在真核细胞中表达活性测定
短句来源
     Construction of a Recombinant Baculovirus Transfer Vector with Two Promoters Expressing the Anti-human CD28 Chimeric Antibody by Using TP-PCR Method
     TP-PCR法构建抗人CD28嵌合抗体双启动子昆虫杆状病毒重组转移载体
短句来源
     TPO expression level was increased from 572 ng/L to 1340 ng/L in the COS-7 cell (P<0.05),and from 783 ng/L to 1040 ng/L in the HC-11 cell (P<0.05) by the vector which have two promoters.
     利用双启动子载体,使人血小板生成素在COS 7细胞由572ng/L提高到1340ng/L(P<0 05); 在HC 11细胞由783ng/L提高1040ng/L(P<0 05)。
短句来源
     Objective To study the expressing activity of vector containing two promoters in eukaryotic cells.
     目的 研究含双启动子表达载体在真核细胞中的表达活性。
短句来源
  “two promoters”译为未确定词的双语例句
     These two promoters, maize ubiquitin promoter and crylle, crylAc gene were used to construct plant expression plasmid p3301cabIeubiAc, p3301silkIe and p3301ubiAc.
     将此两个启动子、玉米ubiquitin启动子与crylIe,crylAc基因分别构建成表达载体p3301cabIeubiAc,p3301silkIe和p3301ubiAc。
短句来源
     AIM: To compare the effect of two promoters in transformation of Dunaliella salina with the chimera gene encoding SBR-CT △A1.METHODS: Plasmid pROUSB with Ubil promoter was constructed.
     目的:比较CaMV35S启动子和Ubil启动子促进编码嵌合体SBR-CT△A1基因转化盐藻细胞的转化效果。
短句来源
     In this study we constructed two promoters (DGP1 and SIGP1) and analyzed their function in transgenic tobaccos.
     鉴于此,本文成功地构建了两个启动子,即DGP1和SIGP1。
短句来源
     The rice endosperm-specific expressing promoter, prolamin promoter and glutelin promoter were cloned from rice total DNA by PCR, these two promoters fused to the beta-glucuronidase(GUS) repoter gene in binary expression vector pBI121 respectively, named pBBP and pBIG; then, we used ipt gene which was isolated from A.
     本研究首先利用PCR法获得了水稻种子专一性表达启动子(醇溶蛋白启动子与谷蛋白启动子),将两启动子插入到植物表达载体pBI121上35S启动子的位置,构建了醇溶蛋白启动子与谷蛋白启动子驱动下的gus基因植物表达载体:随后用ipt基因取代gus基因,分别构建了醇溶蛋白启动子与谷蛋白启动子以及CaMV35S启动子驱动下的ipt基因的植物表达载体。
短句来源
     A study of recombinant dunaliella salina vaccine against dental caries Ⅱ——Comparison of effect of two promoters in transformation ofDunaliella salina with the chimera gene encoding SBR-CT ~(△A1)
     转基因盐藻防龋疫苗的基础研究Ⅱ——两种启动子促进编码嵌合体SBR-CT~(△A1)基因转化盐藻细胞转化效率的比较
短句来源
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  相似匹配句对
     Two.
     二.
短句来源
     Two.
     二、末日意识
短句来源
     Two tissue-specific promoters locate upstream the second exon.
     该基因有两个组织特异性启动子,上游的为胎盘特异启动子,下游为巨噬细胞特异启动子。
短句来源
     Activity Comparison and Mechanism of Two Promoters of Bovine Foamy Virus
     牛泡沫病毒两类启动子活性的比较和机制探讨
短句来源
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  two promoters
These findings indicate that expression of the FHV-1 ICP4 gene is alternatively regulated by the two promoters.
      
In these recombinant virus (rMVAs), GP5 and M proteins were expressed in MVA in the same virus but under the control of two promoters (rMVA-GP5/M), or as a fusion protein under one promoter (rMVA-GP5-M), or separately (rMVA-GP5 and rMVA-M).
      
The expression of the vaccinia IHD-J hemagglutinin (HA) gene is regulated by two promoters, an early/late and a second distinct late promoter.
      
A KP Element Inserted Between the Two Promoters of the Alcohol Dehydrogenase Gene of Drosophila melanogaster Differentially Affe
      
It is proposed that the transcription of the C2 repressor gene initiates at two promoters,pre andprm, similarly to the transcription of thecI gene of lambda phage.
      
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The promoter-less kanamycin resistance gene (neo) from Tn5 has been used todetect promoters in the wide host range Streptomyces multi-copy plasmid pIJ101. Theresults of the experiments suggest that pIJ101 has at least two promoter-active flag-ments which function in E. coli. Analysis of the growth kinetics reveraled that theapparent promoter activity in E. coli is not due to DNA mutation on plJ101 becauseno transient growth inhabition occurs in growing population when kanamycin is added.Two...

The promoter-less kanamycin resistance gene (neo) from Tn5 has been used todetect promoters in the wide host range Streptomyces multi-copy plasmid pIJ101. Theresults of the experiments suggest that pIJ101 has at least two promoter-active flag-ments which function in E. coli. Analysis of the growth kinetics reveraled that theapparent promoter activity in E. coli is not due to DNA mutation on plJ101 becauseno transient growth inhabition occurs in growing population when kanamycin is added.Two promoter-active fragments were located in two DNA fragments of 0. 54 kb and1.18 kb respectively using E. coli promoter-probe plasmid pKK232-8. The subclonesof pIJ101 would be very useful in the detailed studies of this important Sireptomycesplasmid.

用大肠杆菌转座子Tn5上去除了自身启动子区域的卡那霉素抗性基因(neo) 作为指示标记来探测广寄主、高拷贝的链霉菌质粒pIJ101上DNA的启动子活性所做的基因融合试验揭示,pIJ101上至少有两个可在大肠杆菌中行使启动子功能的DNA片段。基因融合后所产生的杂合质粒能够赋予大肠杆菌ED8767较高的卡那霉素抗性。对含质粒菌株在液体培养基中的生长动力学分析揭示,这种菌株在从无药物向有药物的生长环境中过渡时,生长并不受到暂时的抑制。说明这种启动子活性不是pIJ101DNA上DNA的突变所引起。用大肠杆菌的启动子探针载体pKK232-8所做的体外亚克隆试验更进一步证实了这项观察,并把两个启动子功能区分别缩小到0.54kb和1.18kb的范围内。这项试验成功地组建了质粒pIJ101的亚克隆库,便于对这个重要的链霉菌质粒进行精细的分子生物学研究。

The rare earth complex oxide type CO combustion promoter developed and manufac- tured by Peking University was commercially tested in the FCC unit with two-stage regener- ation in the refinery of Yumen Petroleum Administration. It showed that, with the same feedstock, operation conditions and promoter dosage, the effect of CO combustion promotion of the rare earth promoter was equivalent to that of the conventional platinum promoter, the operation of regenerator was smooth, and the No_x content of flue gas was...

The rare earth complex oxide type CO combustion promoter developed and manufac- tured by Peking University was commercially tested in the FCC unit with two-stage regener- ation in the refinery of Yumen Petroleum Administration. It showed that, with the same feedstock, operation conditions and promoter dosage, the effect of CO combustion promotion of the rare earth promoter was equivalent to that of the conventional platinum promoter, the operation of regenerator was smooth, and the No_x content of flue gas was reduced by 59.1%. By comparison of the two promoters, it is believed that the rare earth promoter can be used to replace the platinum promoter in FCC regeneration process.

北京大学研制成功的稀土复合氧化物一氧化碳助燃剂在玉门石油管理局炼油厂的二段再生催化裂化装置上的试验情况表明:在原料性质、操作条件和加剂量不变的情况下,使用该稀土助燃剂的效果与使用铂助燃剂时相当,再生器操作较为平稳,再生烟气中的NO_x含量下降了59.1%,通过与传统的铂助燃剂对比,认为在流化催化裂化再生工艺中,稀土助燃剂可以替代铂助燃剂。

By application of appropriate vectors together with DNA renaturation kinetic method, repetitive DNA sequences of different Cot value were cloned from rice ( Oryza sativa L. ) genome. Two promoter selection plasmids, pKK175-6 and pKK232-8, were used as cloning vectors, which contained promoters Tet or Cat gene, respectively. The recombinant clones acquired resistance to Tc or Cm, and their resistant levels varied from 20μg/ml to 80μg/ml Tc or 30μg/ml to 260μg/ml Cm. The sequence analysis showed that...

By application of appropriate vectors together with DNA renaturation kinetic method, repetitive DNA sequences of different Cot value were cloned from rice ( Oryza sativa L. ) genome. Two promoter selection plasmids, pKK175-6 and pKK232-8, were used as cloning vectors, which contained promoters Tet or Cat gene, respectively. The recombinant clones acquired resistance to Tc or Cm, and their resistant levels varied from 20μg/ml to 80μg/ml Tc or 30μg/ml to 260μg/ml Cm. The sequence analysis showed that there were several regulator- like sequences sxich as prokaryotic and plant-type promoter existed in the cloning fragments.

以大肠杆菌启动子选择质粒pKK175-6、pKK232-8为载体,将通过复性动力学方法获得的水稻重复DNA顺序片段进行克隆。阳性克隆菌株表现出对四环素或氯霉素不同程度的抗性。DNA序列分析表明,克隆到的某些水稻重复DNA顺序具有丰富的基因表达调节元件的同源序列。

 
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